Peroxiredoxin Ⅰ(PrxⅠ) is involved in many important cellular progresses such as cell proliferation and differentiation. To further elucidate its roles in ionizing radiation, eukaryotic expression vector containing s...Peroxiredoxin Ⅰ(PrxⅠ) is involved in many important cellular progresses such as cell proliferation and differentiation. To further elucidate its roles in ionizing radiation, eukaryotic expression vector containing siRNA targeting mRNA of peroxiredoxin 1 gene was constructed and transected into NIH3T3 cell line following sequencing.Western blot analysis showed that this siRNA significantly inhibited PrxⅠ expression by 65% at protein level; cell proliferation experiment demonstrated that the double proliferation time of NIH 3T3PrxⅠ cells was postponed for 1.8 hours as compared with control group. MTT results showed that 12 and 48 hours after irradiation the survival rate of NIH 3T3PrxⅠ was dramatically decreased when comparing with sham-irradiated group.These results suggested that peroxiredoxin 1 has radiation-cytoprotection role.展开更多
文摘Peroxiredoxin Ⅰ(PrxⅠ) is involved in many important cellular progresses such as cell proliferation and differentiation. To further elucidate its roles in ionizing radiation, eukaryotic expression vector containing siRNA targeting mRNA of peroxiredoxin 1 gene was constructed and transected into NIH3T3 cell line following sequencing.Western blot analysis showed that this siRNA significantly inhibited PrxⅠ expression by 65% at protein level; cell proliferation experiment demonstrated that the double proliferation time of NIH 3T3PrxⅠ cells was postponed for 1.8 hours as compared with control group. MTT results showed that 12 and 48 hours after irradiation the survival rate of NIH 3T3PrxⅠ was dramatically decreased when comparing with sham-irradiated group.These results suggested that peroxiredoxin 1 has radiation-cytoprotection role.
