目的探讨辅助生殖技术(assisted reproductive technology,ART)助孕女性经济毒性的现状及其影响因素,为制订针对性干预措施提供依据。方法2024年9-12月,采用便利抽样法选取湖南、广西两省3所三级甲等医院生殖中心就诊的314例ART助孕女...目的探讨辅助生殖技术(assisted reproductive technology,ART)助孕女性经济毒性的现状及其影响因素,为制订针对性干预措施提供依据。方法2024年9-12月,采用便利抽样法选取湖南、广西两省3所三级甲等医院生殖中心就诊的314例ART助孕女性为研究对象,采用患者报告结局经济毒性综合评分量表(comprehensive scores for financial toxicity based on the patient-reported outcome measure,COST-PROM)、中文版不孕症生育压力简式量表(Chinese version of the COMPI fertility problem stress scale,COMPI-FPSS)、感知伴侣回应性量表(perceived partner responsiveness scale,PPRS)、心理弹性量表(Connor-Davidson resilience scale,CD-RISC)对其进行调查。结果ART助孕女性COST-PROM得分为(15.72±7.55)分,其中92.04%(289例)存在经济毒性。多元线性回归分析显示,门诊报销比例、家庭储蓄、助孕次数、异地医疗、ART相关并发症、生育压力、心理弹性是经济毒性的影响因素(均P<0.01),解释总变异的41.74%。结论ART助孕女性普遍存在经济毒性,医护人员应早期识别高危人群,并根据影响因素制订针对性生育护理策略,以降低该人群的经济毒性水平。展开更多
Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established ...Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established by injecting subcutaneously with dehydroepiandrosterone into female Sprague-Dawley rats,followed by receiving intraperitoneal injection of TSG.The granular cells(GCs)KGN were transfected with small interfering RNAs(si-NC and si-CYP19A1).The cells were preincubated with lipopolysaccharide(LPS)and then treated with or without TSG.The estrous cycle was monitored using vaginal exfoliated cells.The morphology of ovarian follicles was analyzed by H&E staining.ELISA was used to analyze estradiol(E2),testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),IL-6,TNF-α,AGEs,CRP and Omentin-1 levels in serum.Immunohistochemistry was performed to analyze PCNA and CYP19A1 expressions in the GCs of ovaries.Tunel staining was executed to detect the apoptosis of GCs.Quantitative polymerase chain reaction(qPCR)and Western blot were implemented to measure the expression of CYP19A1 in the ovaries and transfected cells.qPCR was used to analyze the expression of IL-6 and TNF-αin the transfected cells treated with LPS and TSG.Results The estrous cycles were restored in TSG group.Compared with model group,the sinus follicles were reduced and corpus luteums were increased in TSG group.TSG group showed increased E2,and decreased T and LH,compared with model group.Pro-inflammatory factors(IL-6,TNF-α,CRP and AGEs)were decreased,and anti-inflammatory factor(Omentin-1)was increased in TSG group compared with those in model group.TSG could partially inhibit decrease of PNCA-positive GCs and increase of Tunel-positive GCs caused by PCOS.The CYP19A1 expression of GCs in TSG group was upregulated compared with model group.The expressions of IL-6 and TNFαin si-CYP19A1 cells were increased compared with si-NC cells.Compared with cells(si-NC and si-CYP19A1)treated without LPS,the expressions of IL-6 and TNF-αcells were increased,and the expression of CYP19A1 was downregulated in LPS-preincubated cells.Compared with cells treated with LPS,the expression of IL-6 and TNF-αwere decreased,and the expression of CYP19A1 was increased in cells treated with LPS and TSG.Compared with si-NC cells treated with LPS and TSG,the expressions of IL-6 and TNF-αcells were increased in the si-CYP19A1 cells treated with LPS and TSG.Conclusion TSG could alleviate PCOS-like characteristics by increasing the expression of CYP19A1 in GCs to inhibit inflammatory response.展开更多
文摘目的探讨辅助生殖技术(assisted reproductive technology,ART)助孕女性经济毒性的现状及其影响因素,为制订针对性干预措施提供依据。方法2024年9-12月,采用便利抽样法选取湖南、广西两省3所三级甲等医院生殖中心就诊的314例ART助孕女性为研究对象,采用患者报告结局经济毒性综合评分量表(comprehensive scores for financial toxicity based on the patient-reported outcome measure,COST-PROM)、中文版不孕症生育压力简式量表(Chinese version of the COMPI fertility problem stress scale,COMPI-FPSS)、感知伴侣回应性量表(perceived partner responsiveness scale,PPRS)、心理弹性量表(Connor-Davidson resilience scale,CD-RISC)对其进行调查。结果ART助孕女性COST-PROM得分为(15.72±7.55)分,其中92.04%(289例)存在经济毒性。多元线性回归分析显示,门诊报销比例、家庭储蓄、助孕次数、异地医疗、ART相关并发症、生育压力、心理弹性是经济毒性的影响因素(均P<0.01),解释总变异的41.74%。结论ART助孕女性普遍存在经济毒性,医护人员应早期识别高危人群,并根据影响因素制订针对性生育护理策略,以降低该人群的经济毒性水平。
文摘Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established by injecting subcutaneously with dehydroepiandrosterone into female Sprague-Dawley rats,followed by receiving intraperitoneal injection of TSG.The granular cells(GCs)KGN were transfected with small interfering RNAs(si-NC and si-CYP19A1).The cells were preincubated with lipopolysaccharide(LPS)and then treated with or without TSG.The estrous cycle was monitored using vaginal exfoliated cells.The morphology of ovarian follicles was analyzed by H&E staining.ELISA was used to analyze estradiol(E2),testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),IL-6,TNF-α,AGEs,CRP and Omentin-1 levels in serum.Immunohistochemistry was performed to analyze PCNA and CYP19A1 expressions in the GCs of ovaries.Tunel staining was executed to detect the apoptosis of GCs.Quantitative polymerase chain reaction(qPCR)and Western blot were implemented to measure the expression of CYP19A1 in the ovaries and transfected cells.qPCR was used to analyze the expression of IL-6 and TNF-αin the transfected cells treated with LPS and TSG.Results The estrous cycles were restored in TSG group.Compared with model group,the sinus follicles were reduced and corpus luteums were increased in TSG group.TSG group showed increased E2,and decreased T and LH,compared with model group.Pro-inflammatory factors(IL-6,TNF-α,CRP and AGEs)were decreased,and anti-inflammatory factor(Omentin-1)was increased in TSG group compared with those in model group.TSG could partially inhibit decrease of PNCA-positive GCs and increase of Tunel-positive GCs caused by PCOS.The CYP19A1 expression of GCs in TSG group was upregulated compared with model group.The expressions of IL-6 and TNFαin si-CYP19A1 cells were increased compared with si-NC cells.Compared with cells(si-NC and si-CYP19A1)treated without LPS,the expressions of IL-6 and TNF-αcells were increased,and the expression of CYP19A1 was downregulated in LPS-preincubated cells.Compared with cells treated with LPS,the expression of IL-6 and TNF-αwere decreased,and the expression of CYP19A1 was increased in cells treated with LPS and TSG.Compared with si-NC cells treated with LPS and TSG,the expressions of IL-6 and TNF-αcells were increased in the si-CYP19A1 cells treated with LPS and TSG.Conclusion TSG could alleviate PCOS-like characteristics by increasing the expression of CYP19A1 in GCs to inhibit inflammatory response.
