The injection of bone cement is a promising surgical intervention for the treatment of osteoporosis.The aim of this study was to formulate a novel injectable bioactive bone cement to adress such medical problems.The b...The injection of bone cement is a promising surgical intervention for the treatment of osteoporosis.The aim of this study was to formulate a novel injectable bioactive bone cement to adress such medical problems.The bone cement primarily consists of tricalcium phosphate(β-TCP),konjac glucomannan(KGM),and hydroxyapatite whisker(HAw).An orthogonal experiment was designed to generate multiple sets of new composite calcium phosphate cement(NCPC)samples,and their setting times were measured.The in vitro compatibility of the new bone cement was assessed through relative cell proliferation rate(RGR)and in vitro cell growth experiments.Mechanical strength and porosity tests were conducted for each group of bone cement,and cross-sectional morphology was observed.The results demonstrate that the bone cement exhibits favorable properties such as self-curing,mechanical robustness,and resistance to collapse.The optimum formulation involves a doping ratio of 5/15(wt%)HAw and HA,an additional amount of 1.2wt%KGM,and a liquid citric acid concentration of 2wt%.Porosity tests confirmed that the material has high compressive strength and a favorable porosity of 27%,creating conducive conditions for cell growth,proliferation,and material degradation.Moreover,in vitro cell culture experiments revealed excellent biocompatibility of the material.Consequently,the developed NCPC emerges as a potential candidate material for applications of bone implantation.展开更多
目的:采用两种临床常用粘接剂,对个性化机加工底板托槽、新型网底托槽、双向倒钩底板托槽和传统网底托槽的抗剪切强度(shear bond strength,SBS)进行比较研究,为临床个性化机加工托槽的粘接提供参考。方法:收集48颗新鲜人类前磨牙,随机...目的:采用两种临床常用粘接剂,对个性化机加工底板托槽、新型网底托槽、双向倒钩底板托槽和传统网底托槽的抗剪切强度(shear bond strength,SBS)进行比较研究,为临床个性化机加工托槽的粘接提供参考。方法:收集48颗新鲜人类前磨牙,随机分为8组。A、B组选用个性化机加工底板托槽,C、D组选用新型网底托槽,E、F组选用双向倒钩底板托槽,G、H组选用传统网底托槽;A、C、E、G组使用化学固化粘接剂,B、D、F、H组使用光固化粘接剂。在粘接实验前,在所有托槽中,每种托槽随机选1颗,对其底板在放大40倍、100倍的扫描电镜下进行观察并拍照。使用万能材料实验机,对每组托槽进行SBS的测定,并进行粘接剂残留量(adhesive remnant index,ARI)计分。结果:使用化学固化粘接剂时,其他3种托槽相比,双向倒钩底板托槽粘接强度较低,差异有统计学意义(P<0.05)。使用光固化粘接剂时,双向倒钩底板托槽粘接强度最低,新型网底托槽较低,传统网底托槽和个性化机加工底板托槽粘接强度最高,差异有统计学意义(P<0.05),传统网底托槽和个性化机加工底板托槽之间粘接强度无明显差异(P>0.05)。各组ARI计分差异有统计学意义(P<0.05),进一步比较可得:H组ARI计分最小,且H组与A组和E组的差异有统计学意义(P<0.05)。结论:个性化机加工底板托槽使用光固化粘接剂或使用化学固化粘接剂对其粘接强度无明显影响,粘接强度均能满足正畸临床粘接的要求。H组脱粘接断裂部位相对更接近牙釉质,其余各组牙釉质在脱粘接过程中损伤的风险较小。展开更多
文摘The injection of bone cement is a promising surgical intervention for the treatment of osteoporosis.The aim of this study was to formulate a novel injectable bioactive bone cement to adress such medical problems.The bone cement primarily consists of tricalcium phosphate(β-TCP),konjac glucomannan(KGM),and hydroxyapatite whisker(HAw).An orthogonal experiment was designed to generate multiple sets of new composite calcium phosphate cement(NCPC)samples,and their setting times were measured.The in vitro compatibility of the new bone cement was assessed through relative cell proliferation rate(RGR)and in vitro cell growth experiments.Mechanical strength and porosity tests were conducted for each group of bone cement,and cross-sectional morphology was observed.The results demonstrate that the bone cement exhibits favorable properties such as self-curing,mechanical robustness,and resistance to collapse.The optimum formulation involves a doping ratio of 5/15(wt%)HAw and HA,an additional amount of 1.2wt%KGM,and a liquid citric acid concentration of 2wt%.Porosity tests confirmed that the material has high compressive strength and a favorable porosity of 27%,creating conducive conditions for cell growth,proliferation,and material degradation.Moreover,in vitro cell culture experiments revealed excellent biocompatibility of the material.Consequently,the developed NCPC emerges as a potential candidate material for applications of bone implantation.
文摘目的:采用两种临床常用粘接剂,对个性化机加工底板托槽、新型网底托槽、双向倒钩底板托槽和传统网底托槽的抗剪切强度(shear bond strength,SBS)进行比较研究,为临床个性化机加工托槽的粘接提供参考。方法:收集48颗新鲜人类前磨牙,随机分为8组。A、B组选用个性化机加工底板托槽,C、D组选用新型网底托槽,E、F组选用双向倒钩底板托槽,G、H组选用传统网底托槽;A、C、E、G组使用化学固化粘接剂,B、D、F、H组使用光固化粘接剂。在粘接实验前,在所有托槽中,每种托槽随机选1颗,对其底板在放大40倍、100倍的扫描电镜下进行观察并拍照。使用万能材料实验机,对每组托槽进行SBS的测定,并进行粘接剂残留量(adhesive remnant index,ARI)计分。结果:使用化学固化粘接剂时,其他3种托槽相比,双向倒钩底板托槽粘接强度较低,差异有统计学意义(P<0.05)。使用光固化粘接剂时,双向倒钩底板托槽粘接强度最低,新型网底托槽较低,传统网底托槽和个性化机加工底板托槽粘接强度最高,差异有统计学意义(P<0.05),传统网底托槽和个性化机加工底板托槽之间粘接强度无明显差异(P>0.05)。各组ARI计分差异有统计学意义(P<0.05),进一步比较可得:H组ARI计分最小,且H组与A组和E组的差异有统计学意义(P<0.05)。结论:个性化机加工底板托槽使用光固化粘接剂或使用化学固化粘接剂对其粘接强度无明显影响,粘接强度均能满足正畸临床粘接的要求。H组脱粘接断裂部位相对更接近牙釉质,其余各组牙釉质在脱粘接过程中损伤的风险较小。
