Mitigation effects of coenzyme Q10(CoQ10)on spiromesifen(SPM)-induced acute toxicity to zebrafish at different life stages,as well as abnormal development,especially impaired development of heart and notochord,oxidati...Mitigation effects of coenzyme Q10(CoQ10)on spiromesifen(SPM)-induced acute toxicity to zebrafish at different life stages,as well as abnormal development,especially impaired development of heart and notochord,oxidative stress and apoptosis in embryos were investigated using a semi-static method.Results showed that CoQ10 can remarkably reduce the acute toxicity of SPM to zebrafish embryos,larvae and adults by 3.20-,2.09-and 1.51-fold,respectively.CoQ10 can effectively alleviate the SPM-induced abnormal spontaneous movement,decreased heartbeat,delayed hatching,growth inhibition and various teratogenic effects of zebrafish embryos.At the stage of zebrafish embryo,CoQ10 significantly increases superoxide dismutase(SOD)activity and expression levels of genes related to anti-oxidation(Cat&Cu/Zn-sod),anti-apoptosis(mcl1a&mcl1b),heart development(cmlc1,actc1a&erbb4a)and angiogenesis(vegfaa,vegfd&kdrl),which are inhibited by SPM.Additionally,CoQ10 reduces malondialdehyde(MDA)content and caspase-3 activity of embryos,as well as expression levels of pro-apoptosis(Cas3,Cas9,P53&bax)and notochord(tbxta&col2a)related genes raised by SPM.The results indicate that CoQ10 can mitigate the acute toxicity of SPM to zebrafish,and alleviate the abnormal development of heart and notochord of embryos by reducing oxidative stress and apoptosis,as well as regulating the expression of related genes.展开更多
建立液相色谱质谱联用快速检测菜薹中丙溴磷残留的分析方法,并对丙溴磷在菜薹中的残留消解及对人体的膳食摄入风险进行了研究。结果表明:丙溴磷在菜薹中半衰期为1.3~4.7 d。40%丙溴磷乳油,按照360 g a.i./hm^2和450 g a.i./hm^2用量,喷...建立液相色谱质谱联用快速检测菜薹中丙溴磷残留的分析方法,并对丙溴磷在菜薹中的残留消解及对人体的膳食摄入风险进行了研究。结果表明:丙溴磷在菜薹中半衰期为1.3~4.7 d。40%丙溴磷乳油,按照360 g a.i./hm^2和450 g a.i./hm^2用量,喷雾施药2~3次,施药间隔7 d,距末次施药后间隔5 d采样,菜薹中丙溴磷的残留中值为1.69 mg/kg。膳食风险评估结果表明:丙溴磷在所有登记作物中的总膳食摄入风险商(RQ)为0.30。膳食风险在可接受范围,说明丙溴磷在菜薹中残留不会对国人健康产生影响。展开更多
为明确高效氟吡甲禾灵、三氟羧草醚的联合作用类型及和25%高氟吡·三氟羧草醚乳油对花生田杂草的防除效果以及对花生的安全性,采用茎叶喷雾法测定了2种除草剂室内联合作用类型,并对筛选的25%高氟吡·三氟羧草醚乳油进行了田间...为明确高效氟吡甲禾灵、三氟羧草醚的联合作用类型及和25%高氟吡·三氟羧草醚乳油对花生田杂草的防除效果以及对花生的安全性,采用茎叶喷雾法测定了2种除草剂室内联合作用类型,并对筛选的25%高氟吡·三氟羧草醚乳油进行了田间防效验证。结果表明,高效氟吡甲禾灵、三氟羧草醚以1∶4复配对防除花生田一年生禾本科杂草有增效作用,25%高氟吡·三氟羧草醚乳油的推荐使用剂量为150.0~187.5 g a.i./hm^(2),药后46 d对杂草的总鲜质量防效可达到92.82%~95.72%。高效氟吡甲禾灵、三氟羧草醚复配对防除狗尾草等杂草增效作用明显,25%高氟吡·三氟羧草醚乳油能够有效防除花生田一年生杂草,并对花生安全。展开更多
基金Supported by National Key Research and Development Program of China(No.2017YFD0200504)。
文摘Mitigation effects of coenzyme Q10(CoQ10)on spiromesifen(SPM)-induced acute toxicity to zebrafish at different life stages,as well as abnormal development,especially impaired development of heart and notochord,oxidative stress and apoptosis in embryos were investigated using a semi-static method.Results showed that CoQ10 can remarkably reduce the acute toxicity of SPM to zebrafish embryos,larvae and adults by 3.20-,2.09-and 1.51-fold,respectively.CoQ10 can effectively alleviate the SPM-induced abnormal spontaneous movement,decreased heartbeat,delayed hatching,growth inhibition and various teratogenic effects of zebrafish embryos.At the stage of zebrafish embryo,CoQ10 significantly increases superoxide dismutase(SOD)activity and expression levels of genes related to anti-oxidation(Cat&Cu/Zn-sod),anti-apoptosis(mcl1a&mcl1b),heart development(cmlc1,actc1a&erbb4a)and angiogenesis(vegfaa,vegfd&kdrl),which are inhibited by SPM.Additionally,CoQ10 reduces malondialdehyde(MDA)content and caspase-3 activity of embryos,as well as expression levels of pro-apoptosis(Cas3,Cas9,P53&bax)and notochord(tbxta&col2a)related genes raised by SPM.The results indicate that CoQ10 can mitigate the acute toxicity of SPM to zebrafish,and alleviate the abnormal development of heart and notochord of embryos by reducing oxidative stress and apoptosis,as well as regulating the expression of related genes.
文摘建立液相色谱质谱联用快速检测菜薹中丙溴磷残留的分析方法,并对丙溴磷在菜薹中的残留消解及对人体的膳食摄入风险进行了研究。结果表明:丙溴磷在菜薹中半衰期为1.3~4.7 d。40%丙溴磷乳油,按照360 g a.i./hm^2和450 g a.i./hm^2用量,喷雾施药2~3次,施药间隔7 d,距末次施药后间隔5 d采样,菜薹中丙溴磷的残留中值为1.69 mg/kg。膳食风险评估结果表明:丙溴磷在所有登记作物中的总膳食摄入风险商(RQ)为0.30。膳食风险在可接受范围,说明丙溴磷在菜薹中残留不会对国人健康产生影响。
文摘为明确高效氟吡甲禾灵、三氟羧草醚的联合作用类型及和25%高氟吡·三氟羧草醚乳油对花生田杂草的防除效果以及对花生的安全性,采用茎叶喷雾法测定了2种除草剂室内联合作用类型,并对筛选的25%高氟吡·三氟羧草醚乳油进行了田间防效验证。结果表明,高效氟吡甲禾灵、三氟羧草醚以1∶4复配对防除花生田一年生禾本科杂草有增效作用,25%高氟吡·三氟羧草醚乳油的推荐使用剂量为150.0~187.5 g a.i./hm^(2),药后46 d对杂草的总鲜质量防效可达到92.82%~95.72%。高效氟吡甲禾灵、三氟羧草醚复配对防除狗尾草等杂草增效作用明显,25%高氟吡·三氟羧草醚乳油能够有效防除花生田一年生杂草,并对花生安全。
