A novel non-enzymatic method for the determination of oxidases and their sub-strates was proposed. The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA (NH4)2Fe (SO4), as modifi...A novel non-enzymatic method for the determination of oxidases and their sub-strates was proposed. The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA (NH4)2Fe (SO4), as modified Fenton reagent, H2O2, the prod-uct of GOD catalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GOD was thus measured according to the reaction rates. The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-展开更多
文摘A novel non-enzymatic method for the determination of oxidases and their sub-strates was proposed. The principle was examined by fluorometric determination of glucose oxidase(GOD). Using EGTA (NH4)2Fe (SO4), as modified Fenton reagent, H2O2, the prod-uct of GOD catalytic reaction was converted into hydroxyl radical, the later oxidized tereph-thalic acid, which was chosen from four fluorogenic indicator of hydroxyl radical, into fluo-rescent product GOD was thus measured according to the reaction rates. The rnethod is sim-ple, stable, cheap and at least as sensitive as its counterpart enzymatic method-
