目的 探讨雌激素通过雌激素受体 (estrogen receptor ER )调节细胞凋亡的可能机制。方法 利用荧光免疫组织化学的方法,研究了 10例阿尔茨海默病 Alzheim er's disease AD 患者和 10例对照受试者海马中 Bcl-2的分布及 Bcl-2和 ER α...目的 探讨雌激素通过雌激素受体 (estrogen receptor ER )调节细胞凋亡的可能机制。方法 利用荧光免疫组织化学的方法,研究了 10例阿尔茨海默病 Alzheim er's disease AD 患者和 10例对照受试者海马中 Bcl-2的分布及 Bcl-2和 ER α的共存现象。结果 Bcl-2主要在 AD 患者和对照受试者海马 CA 3和 CA 4亚区的锥体层神经元中广泛表达,且多分布于胞质和突起,胞核较少。星型胶质细胞中也可检测到 Bcl-2免疫活性,AD 组中大量表达,而对照组中很少表达。免疫组织化学荧光双标显示,大部分被 Bcl-2免疫标记的神经元也表达 ER α。结论 在 AD 患者海马的神经元和星型胶质细胞中,雌激素可能作为细胞凋亡的一个调节子,通过 ER α来调节 Bcl-2表达,行使其神经保护作用。展开更多
A new copolymer(C) was designed and synthesized.Isothermal Titration Calorimetry(ITC) expe-riments showed the strong binding of C-Zn and the predetermined peptide sequences(DFLAE,appear in many peptides sequence which...A new copolymer(C) was designed and synthesized.Isothermal Titration Calorimetry(ITC) expe-riments showed the strong binding of C-Zn and the predetermined peptide sequences(DFLAE,appear in many peptides sequence which cumulate in serum of uremic patients).The further ITC experiments demonstrate that the binding is the result of the cooperative effect of coordination and hydrophobic interaction,which may provide further understanding on the mechanism of polymer-metal-peptide interaction and a structural basis for designing the adsorbents for the target peptide.展开更多
文摘目的 探讨雌激素通过雌激素受体 (estrogen receptor ER )调节细胞凋亡的可能机制。方法 利用荧光免疫组织化学的方法,研究了 10例阿尔茨海默病 Alzheim er's disease AD 患者和 10例对照受试者海马中 Bcl-2的分布及 Bcl-2和 ER α的共存现象。结果 Bcl-2主要在 AD 患者和对照受试者海马 CA 3和 CA 4亚区的锥体层神经元中广泛表达,且多分布于胞质和突起,胞核较少。星型胶质细胞中也可检测到 Bcl-2免疫活性,AD 组中大量表达,而对照组中很少表达。免疫组织化学荧光双标显示,大部分被 Bcl-2免疫标记的神经元也表达 ER α。结论 在 AD 患者海马的神经元和星型胶质细胞中,雌激素可能作为细胞凋亡的一个调节子,通过 ER α来调节 Bcl-2表达,行使其神经保护作用。
文摘A new copolymer(C) was designed and synthesized.Isothermal Titration Calorimetry(ITC) expe-riments showed the strong binding of C-Zn and the predetermined peptide sequences(DFLAE,appear in many peptides sequence which cumulate in serum of uremic patients).The further ITC experiments demonstrate that the binding is the result of the cooperative effect of coordination and hydrophobic interaction,which may provide further understanding on the mechanism of polymer-metal-peptide interaction and a structural basis for designing the adsorbents for the target peptide.
