目的检索鼻咽癌患者口咽部症状护理相关指南,并进行质量评价和内容分析,为临床提供参考。方法系统检索指南网、头颈部肿瘤相关学会、数据库等,检索日期为建库至2022年3月1日。采用临床指南研究和评价系统(the appraisal of guidelines f...目的检索鼻咽癌患者口咽部症状护理相关指南,并进行质量评价和内容分析,为临床提供参考。方法系统检索指南网、头颈部肿瘤相关学会、数据库等,检索日期为建库至2022年3月1日。采用临床指南研究和评价系统(the appraisal of guidelines for research evaluation instrument,AGREE II)对指南进行质量评价。结果共纳入4篇指南、3篇专家共识。指南总体质量评价为1篇A级、3篇B级,专家共识真实性较好。提取6个方面内容:放射性口腔干燥症的应对、放射性口腔黏膜炎及感染的管理、放射性龋齿的护理、张口受限的训练、吞咽障碍的干预、随访管理,共23条推荐意见。结论纳入的鼻咽癌口咽部症状护理相关的指南总体质量中等,其指南开发的参与人员、严谨性、应用性和独立性领域均有待提高。目前我国尚缺乏高质量的鼻咽癌患者口咽部症状护理相关实践指南,整合的推荐意见具有较好的实践意义。建议在借鉴国外高质量指南的基础上制定本土化循证指南,以指导临床实践。展开更多
目的通过总结诺尔康人工耳蜗在特殊病例中的应用情况,客观评价诺尔康人工耳蜗在特殊类型感音神经性耳聋患者中的有效性及安全性,为相关患者人工耳蜗植入提供参考。方法对大前庭导水管综合征、脑白质营养不良、耳硬化症、慢性化脓性中耳...目的通过总结诺尔康人工耳蜗在特殊病例中的应用情况,客观评价诺尔康人工耳蜗在特殊类型感音神经性耳聋患者中的有效性及安全性,为相关患者人工耳蜗植入提供参考。方法对大前庭导水管综合征、脑白质营养不良、耳硬化症、慢性化脓性中耳炎、双侧植入、再次植入等特殊病例患者,比较分析接受诺尔康人工耳蜗植入过程与类似病例在其他人工耳蜗植入过程中的异同点,术后定期调试及听觉言语测试评价其安全性及听觉言语康复效果,总结诺尔康人工耳蜗在特殊病例人工耳蜗植入中的特点并进行疗效分析。结果 13例14耳特殊病例患者均成功植入诺尔康人工耳蜗且无并发症。诺尔康人工耳蜗特殊病例组患者术后声场测听平均听阈为25~35 dB HL,均达到日常生活实用听力水平。术后开机3个月声母、韵母、单音节词言语识别率分别为52.33±10.95%、62.32±11.01%及40.11±9.43%,与诺尔康普通病例组及某国外品牌对照组术后言语识别率无统计学差异(P>0.05)。结论诺尔康人工耳蜗在产品安全性及有效性方面和国外产品并无显著差别,可常规应用于特殊病例的人工耳蜗植入手术。展开更多
Objective Our previous study has revealed that iASPP is elevated in human head and neck squamous cell carcinoma(HNSCC)and iASPP overexpression signifcantly correlates with tumor malignant progression and poor survival...Objective Our previous study has revealed that iASPP is elevated in human head and neck squamous cell carcinoma(HNSCC)and iASPP overexpression signifcantly correlates with tumor malignant progression and poor survival of HNSCC.This study investigated the function of iASPP playing in proliferation and invasion of HNSCC in vitro.Methods HNSCC cell line Tu686 transfected with Lentiviral vector-mediated iASPP-specific shRNA and control shRNA were named the shRNA-iASPP group and shRNA-NC group,respectively.The non-infected Tu686 cells were named the CON group.CCK-8 assay,flow cytometry,transwell invasion assay were performed to detect the effects of iASPP inhibition in vitro.Results Our results demonstrated that the proliferation of shRNA-iASPP cells at the time of 72 h(F=32.459,P=0.000),96 h(F=51.407,P=0.000),120 h(F=35.125,P=0.000)post-transfection,was significantly lower than that of shRNANC cells and CON cells.The apoptosis ratio of shRNA-iASPP cells was 9.42%±0.39%(F=299.490,P=0.000),which was significantly higher than that of CON cells(2.80%±0.42%)and shRNA-NC cells(3.18%±0.28%).The percentage of shRNA-iASPP cells in G0/G1 phase was 74.65%±1.09%(F=388.901,P=0.000),which was strikingly increased,compared with that of CON cells(55.19%±1.02%)and shRNA-NC cells(54.62%±0.88%).The number of invading cells was 56±4 in the shRNA-iASPP group(F=84.965,P=0.000),which decreased significantly,compared with the CON group(111±3)and the shRNA-NC group(105±8).The survival rate of shRNA-iASPP cells administrated with paclitaxel was highly decreased,compared with CON cells and shRNA-NC cells(F=634.841,P=0.000).Conclusion These results suggest iASPP may play an important role in progression and aggressive behavior of HNSCC and may be an efficient chemotherapeutic target for the treatment of HNSCC.展开更多
文摘目的检索鼻咽癌患者口咽部症状护理相关指南,并进行质量评价和内容分析,为临床提供参考。方法系统检索指南网、头颈部肿瘤相关学会、数据库等,检索日期为建库至2022年3月1日。采用临床指南研究和评价系统(the appraisal of guidelines for research evaluation instrument,AGREE II)对指南进行质量评价。结果共纳入4篇指南、3篇专家共识。指南总体质量评价为1篇A级、3篇B级,专家共识真实性较好。提取6个方面内容:放射性口腔干燥症的应对、放射性口腔黏膜炎及感染的管理、放射性龋齿的护理、张口受限的训练、吞咽障碍的干预、随访管理,共23条推荐意见。结论纳入的鼻咽癌口咽部症状护理相关的指南总体质量中等,其指南开发的参与人员、严谨性、应用性和独立性领域均有待提高。目前我国尚缺乏高质量的鼻咽癌患者口咽部症状护理相关实践指南,整合的推荐意见具有较好的实践意义。建议在借鉴国外高质量指南的基础上制定本土化循证指南,以指导临床实践。
文摘目的通过总结诺尔康人工耳蜗在特殊病例中的应用情况,客观评价诺尔康人工耳蜗在特殊类型感音神经性耳聋患者中的有效性及安全性,为相关患者人工耳蜗植入提供参考。方法对大前庭导水管综合征、脑白质营养不良、耳硬化症、慢性化脓性中耳炎、双侧植入、再次植入等特殊病例患者,比较分析接受诺尔康人工耳蜗植入过程与类似病例在其他人工耳蜗植入过程中的异同点,术后定期调试及听觉言语测试评价其安全性及听觉言语康复效果,总结诺尔康人工耳蜗在特殊病例人工耳蜗植入中的特点并进行疗效分析。结果 13例14耳特殊病例患者均成功植入诺尔康人工耳蜗且无并发症。诺尔康人工耳蜗特殊病例组患者术后声场测听平均听阈为25~35 dB HL,均达到日常生活实用听力水平。术后开机3个月声母、韵母、单音节词言语识别率分别为52.33±10.95%、62.32±11.01%及40.11±9.43%,与诺尔康普通病例组及某国外品牌对照组术后言语识别率无统计学差异(P>0.05)。结论诺尔康人工耳蜗在产品安全性及有效性方面和国外产品并无显著差别,可常规应用于特殊病例的人工耳蜗植入手术。
基金Supported by Beijing Medical Health Public Welfare Foundation(grant no.YWJKJJHKYJJ-B17468 and running period:2017.09.01-2020.09.01)
文摘Objective Our previous study has revealed that iASPP is elevated in human head and neck squamous cell carcinoma(HNSCC)and iASPP overexpression signifcantly correlates with tumor malignant progression and poor survival of HNSCC.This study investigated the function of iASPP playing in proliferation and invasion of HNSCC in vitro.Methods HNSCC cell line Tu686 transfected with Lentiviral vector-mediated iASPP-specific shRNA and control shRNA were named the shRNA-iASPP group and shRNA-NC group,respectively.The non-infected Tu686 cells were named the CON group.CCK-8 assay,flow cytometry,transwell invasion assay were performed to detect the effects of iASPP inhibition in vitro.Results Our results demonstrated that the proliferation of shRNA-iASPP cells at the time of 72 h(F=32.459,P=0.000),96 h(F=51.407,P=0.000),120 h(F=35.125,P=0.000)post-transfection,was significantly lower than that of shRNANC cells and CON cells.The apoptosis ratio of shRNA-iASPP cells was 9.42%±0.39%(F=299.490,P=0.000),which was significantly higher than that of CON cells(2.80%±0.42%)and shRNA-NC cells(3.18%±0.28%).The percentage of shRNA-iASPP cells in G0/G1 phase was 74.65%±1.09%(F=388.901,P=0.000),which was strikingly increased,compared with that of CON cells(55.19%±1.02%)and shRNA-NC cells(54.62%±0.88%).The number of invading cells was 56±4 in the shRNA-iASPP group(F=84.965,P=0.000),which decreased significantly,compared with the CON group(111±3)and the shRNA-NC group(105±8).The survival rate of shRNA-iASPP cells administrated with paclitaxel was highly decreased,compared with CON cells and shRNA-NC cells(F=634.841,P=0.000).Conclusion These results suggest iASPP may play an important role in progression and aggressive behavior of HNSCC and may be an efficient chemotherapeutic target for the treatment of HNSCC.
