目的:初步探讨proBDNF(precursor of brain-derived neurotrophic factor)对海马神经元的可能作用及其机制。方法:采用体外培养胚鼠海马神经元,并给予proBDNF,preBDNF(propeptide of proBDNF)和抗proBDNF血清处理;30min,1h和48h后行尼...目的:初步探讨proBDNF(precursor of brain-derived neurotrophic factor)对海马神经元的可能作用及其机制。方法:采用体外培养胚鼠海马神经元,并给予proBDNF,preBDNF(propeptide of proBDNF)和抗proBDNF血清处理;30min,1h和48h后行尼氏染色,ELK-p(Ets domain protein),ErK2(extracellularsignal regulated kinase)和c-fos免疫细胞化学染色。结果:给予proBDNF处理后,可见培养细胞内ELK-p,ErK2和c-fos表达明显上调,部分细胞核深染;而用抗血清中和内源性proBDNF后,ELK-p、ErK2和c-fos均下调,且细胞出现肿胀、空泡样变化。preBDNF处理组细胞与正常对照组类似。结论:proBDNF可维持海马神经元的存活,其机制可能与激活ErK和ELK信号通路有关。展开更多
