摘要
目的：检测脑源性神经营养因子(BDNF)干预后大鼠视网膜TrkB的表达变化。为受损后视网膜节细胞的保护及外源性BDNF的应用提供一定的理论基础。方法：大鼠左眼分为急性眼高压及BDNF预处理组。使左眼眼压升高至闪光视网膜电图b波消失的临界眼压并维持60 min,分别存活1～14 d后处死,冰冻切片行尼氏染色及TrkB的免疫组织化学。结果：急性高眼压组各时间点节细胞层细胞数目均显著少于BDNF预处理组；1、3 d时TrkB的表达明显增加,7、14 d时则明显减少；BDNF预处理组在存活1、3 d时TrkB的表达明显增加,7 d时则下降至正常对照组水平,14 d时再次明显增加。结论：急性高眼压后大鼠视网膜内TrkB的表达存在时空变化,TrkB表达的上调提示视网膜对BDNF的需求增加。
Objective： To investigate the expression of TrkB in rat retina following acute high intraocular pressure （HIOP） induced by brain-derived neurotrophie factor （BDNF）. Methods： Forty-eight adult rats were randomly divided into acute HIOP group and BDNF pro-treated group. The left eyes of rats in BDNF pre-treated group were injected with BDNF 2 days before HIOP. The intraoeular pressure of all left eyes was increased until b wave of flash eleetroretinogragh （fERG） disappeared and such pressure maintained for 60 minutes. The right eyes served as a normal control group. The rats were sacrificed after 1, 3, 7 and 14 days. Nissl staining and immuno histoehemistry for detecting the expression of TrkB were used. Results： The arrangement of the retinal neurons was clearer and more regular in BDNF pre-treated group than that in HIOP group. Compared to the normal control group, the expression of TrkB in HIOP group was significantly increased at day 1 and 3, then decreased gradually. The expression of TrkB in BDNF pro〉treated group was similar to that in HIOP group at day 1 and 3, but restored to the level of the normal control group at day 7, and again significantly elevated at day 14. Conclusion： Temporal and spatial changes in the expression of TrkB occur in rat retina following acute HIOP. The up regulation of TrkB expression suggests that more BDNFs are required in such a situation.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2006年第6期734-737,740,共5页
Chinese Journal of Anatomy
基金
国家自然科学基金(30100098)
关键词
急性眼高压
脑源性神经营养因子
TRKB
视网膜
acute high intraoeular pressure
brain-derived neurotrophie factor
TrkB
retina
作者简介
Email：huangjufang@mail．cus．edu．cn
