In the criminal cases of driving under the influence(DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis.The evidence can be acquired...In the criminal cases of driving under the influence(DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis.The evidence can be acquired when the skin cells are observed on the surface of the airbag in a traffic accident. However, the low quantity or quality of the evidence collected from a crime scene prevents further identification analysis in many cases. In the current study, we reported a case of identifying touch DNA extraction from the shed skin cells from the deployed airbag of a motor vehicle. We managed to collect DNA evidence from the shed skin cells in an airbag using a proper approach of collection and extraction. The 5.87 ng of extracted DNA was sufficient for genotyping and forensic identification, which helped to identify the driver of the car in collision with a pier in the street. In DUI cases and other traffic accidents, therefore, the amount of touch DNA extracted from the deployed airbag can be sufficient for DNA marker genotyping and further analysis.展开更多
为探究种植菌草的丘陵地区土壤间、土壤与移栽触土部件间相互作用的规律并获取其仿真参数,运用Hertz—Mindlin with JKR接触模型对特定的12%±1%和20%±1%的含水率土壤—移栽触土部件进行离散元参数标定。开展土壤堆积角物理试...为探究种植菌草的丘陵地区土壤间、土壤与移栽触土部件间相互作用的规律并获取其仿真参数,运用Hertz—Mindlin with JKR接触模型对特定的12%±1%和20%±1%的含水率土壤—移栽触土部件进行离散元参数标定。开展土壤堆积角物理试验、土球斜面滚动物理试验。以土壤颗粒间、土壤与移栽触土部件间的表面能、恢复系数、动摩擦系数、静摩擦系数为标定对象,设计旋转中心组合试验并以仿真的土壤堆积角、土球在65Mn板上滚动距离为响应值,采用Box—Behnken对试验数据回归分析,以实测的土壤堆积角、土球滚动距离为优化目标,采用两种典型含水率下土壤与触土材料(65Mn)的静摩擦系数为约束条件,得到两种典型含水率的土壤间、土壤与移栽触土部件的离散元参数:含水率分别为12%±1%、20%±1%时,土壤颗粒间表面能、恢复系数、动摩擦系数、静摩擦系数为11.042 J/m^(2)和11.851 J/m^(2)、0.412和0.574、0.093和0.129、0.994和1.009;土壤与触土部件表面能、恢复系数、动摩擦系数为5.046 J/m^(2)、8.026 J/m^(2),0.362、0.388和0.066、0.07。为验证优化后离散元参数的准确性,开展验证试验得:两种典型含水率土壤仿真、物理堆积角相对误差为0.96%、0.95%,仿真、物理滚动试验相对误差为0.52%、1%。结果表明,优化标定后的土壤模型参数能够仿真该地区真实的菌草土壤模型,可为菌草移栽械关键部件的设计与优化提供重要理论依据。展开更多
基金This research was supported by the National Natural Science Foundation of China
文摘In the criminal cases of driving under the influence(DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis.The evidence can be acquired when the skin cells are observed on the surface of the airbag in a traffic accident. However, the low quantity or quality of the evidence collected from a crime scene prevents further identification analysis in many cases. In the current study, we reported a case of identifying touch DNA extraction from the shed skin cells from the deployed airbag of a motor vehicle. We managed to collect DNA evidence from the shed skin cells in an airbag using a proper approach of collection and extraction. The 5.87 ng of extracted DNA was sufficient for genotyping and forensic identification, which helped to identify the driver of the car in collision with a pier in the street. In DUI cases and other traffic accidents, therefore, the amount of touch DNA extracted from the deployed airbag can be sufficient for DNA marker genotyping and further analysis.
