RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The n...RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The neonate larvae of SPB are endophagous,initially feeding on the inner layer of the pod and then on the immature soybean seeds,a behavior that makes them difficult to rear.Therefore,a suitable artificial diet is desirable to facilitate RNAi research.Seven artificial diets and soybean pods were used for rearing SPB neonate larvae.The survival and mean weight of the larvae reared on artificial diet 6(A6,included 2 g soybean flour and 10 g R3 immature soybean pod powder)were found to be significantly higher than those for larvae reared on all other diets.Subsequently,A6 diet was prepared in DEPC-treated water to obtain a RNase-free artificial diet(RF).The survival and mean weight of the larvae were not significantly different between A6 and RF diets.Feeding neonate larvae with RF-SpbP0 dsRNA(ribosomal protein P0)silenced SpbP0 gene,resulting in arrested development and increased mortality.The results of this study suggested that the newly developed A6 or RF diets could rear SPB neonate larvae and help towards developing an effective method for oral delivery of dsRNA to SPB larvae.展开更多
1.大豆果实发育过程中种子和荚皮过氧化物酶(Peroxidase-POD,下同)和超氧化物岐化酶(superoxide Dismutase—SOD,下同)活性均有变化。结瘤大豆的两种酶活性高于非结瘤大豆,这种差异在 POD 活性上表现尤为显著。2.SOD 同工酶在种子发育...1.大豆果实发育过程中种子和荚皮过氧化物酶(Peroxidase-POD,下同)和超氧化物岐化酶(superoxide Dismutase—SOD,下同)活性均有变化。结瘤大豆的两种酶活性高于非结瘤大豆,这种差异在 POD 活性上表现尤为显著。2.SOD 同工酶在种子发育早期就基本合成齐全,而 POD 同工酶随发育逐渐增多。3.种子 SOD 和 POD 均高于荚皮中的活性,但同工酶带数却以荚皮的为多,而且非结瘤种子和荚皮的两种同工酶条带均多于结瘤株的。这表明同工酶条带数与活性间不一定呈相关关系。展开更多
为探究野大豆(Glycine soja Sieb)荚果形成及种子萌发特性,以湖南省长沙市浏阳市野生状态下的野大豆群落为试验材料,对野大豆不同生殖生长期荚果生长发育变化、种子变化、千粒重等指标进行观测。结果表明,野大豆生殖生长时期为42 d,植...为探究野大豆(Glycine soja Sieb)荚果形成及种子萌发特性,以湖南省长沙市浏阳市野生状态下的野大豆群落为试验材料,对野大豆不同生殖生长期荚果生长发育变化、种子变化、千粒重等指标进行观测。结果表明,野大豆生殖生长时期为42 d,植株上层种子先于植株下层种子成熟,为无限结荚习性,种子千粒重7.0712~7.1666g。采用双因素试验设计,探讨不同贮藏时间和是否划破种皮对种子萌发特性的影响,结果表明,划破种皮种子发芽参数显著高于未划破种皮种子处理组(P<0.05);贮藏时间为120 d时划破种皮种子与未划破种皮种子的发芽率、发芽势和发芽指数显著高于其他处理组。该结果有助于指导野大豆种质资源的收集和保存。展开更多
The resaerch examined the effect of the two oceanic materials as coating materials on the soybean growth.The results showed chitosan and sodium alginate seed coating can enhance the growth of seedling root,increase th...The resaerch examined the effect of the two oceanic materials as coating materials on the soybean growth.The results showed chitosan and sodium alginate seed coating can enhance the growth of seedling root,increase the nodule mumber,root activity and the growth of underground.The suggested coating ratios were 0.5～1.0g/kgseed,the same as chitosan.The two materials could increase the contents of CAT and NR in soybean leaves,decrease the contents of POD in soybean leaves.展开更多
基金Supported by the Harbin Science and Technology Innovation Program(2017RAQXJ122)China National Novel Transgenic Organisms Breeding Project(2016ZX08004-004-006)+2 种基金the Heilongjiang Natural Science Foundation(C2018012)the Chinese National Natural Science Foundation(31201229)Soybean Research&Development Center(CARS-04-PS04)。
文摘RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The neonate larvae of SPB are endophagous,initially feeding on the inner layer of the pod and then on the immature soybean seeds,a behavior that makes them difficult to rear.Therefore,a suitable artificial diet is desirable to facilitate RNAi research.Seven artificial diets and soybean pods were used for rearing SPB neonate larvae.The survival and mean weight of the larvae reared on artificial diet 6(A6,included 2 g soybean flour and 10 g R3 immature soybean pod powder)were found to be significantly higher than those for larvae reared on all other diets.Subsequently,A6 diet was prepared in DEPC-treated water to obtain a RNase-free artificial diet(RF).The survival and mean weight of the larvae were not significantly different between A6 and RF diets.Feeding neonate larvae with RF-SpbP0 dsRNA(ribosomal protein P0)silenced SpbP0 gene,resulting in arrested development and increased mortality.The results of this study suggested that the newly developed A6 or RF diets could rear SPB neonate larvae and help towards developing an effective method for oral delivery of dsRNA to SPB larvae.
文摘1.大豆果实发育过程中种子和荚皮过氧化物酶(Peroxidase-POD,下同)和超氧化物岐化酶(superoxide Dismutase—SOD,下同)活性均有变化。结瘤大豆的两种酶活性高于非结瘤大豆,这种差异在 POD 活性上表现尤为显著。2.SOD 同工酶在种子发育早期就基本合成齐全,而 POD 同工酶随发育逐渐增多。3.种子 SOD 和 POD 均高于荚皮中的活性,但同工酶带数却以荚皮的为多,而且非结瘤种子和荚皮的两种同工酶条带均多于结瘤株的。这表明同工酶条带数与活性间不一定呈相关关系。
文摘为探究野大豆(Glycine soja Sieb)荚果形成及种子萌发特性,以湖南省长沙市浏阳市野生状态下的野大豆群落为试验材料,对野大豆不同生殖生长期荚果生长发育变化、种子变化、千粒重等指标进行观测。结果表明,野大豆生殖生长时期为42 d,植株上层种子先于植株下层种子成熟,为无限结荚习性,种子千粒重7.0712~7.1666g。采用双因素试验设计,探讨不同贮藏时间和是否划破种皮对种子萌发特性的影响,结果表明,划破种皮种子发芽参数显著高于未划破种皮种子处理组(P<0.05);贮藏时间为120 d时划破种皮种子与未划破种皮种子的发芽率、发芽势和发芽指数显著高于其他处理组。该结果有助于指导野大豆种质资源的收集和保存。
文摘The resaerch examined the effect of the two oceanic materials as coating materials on the soybean growth.The results showed chitosan and sodium alginate seed coating can enhance the growth of seedling root,increase the nodule mumber,root activity and the growth of underground.The suggested coating ratios were 0.5～1.0g/kgseed,the same as chitosan.The two materials could increase the contents of CAT and NR in soybean leaves,decrease the contents of POD in soybean leaves.
