期刊文献+
共找到3,360篇文章
< 1 2 168 >
每页显示 20 50 100
Special secret sharing scheme with the function of assignment 被引量:3
1
作者 叶振军 孟繁桢 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2005年第3期651-653,共3页
A concept of secret sharing scheme with the function of assignment is proposed. It provides great capabilities for many practical applications. In this scheme, the dealer can randomly assign one or more than one parti... A concept of secret sharing scheme with the function of assignment is proposed. It provides great capabilities for many practical applications. In this scheme, the dealer can randomly assign one or more than one participant to get the secret at any time, but these participants can get nothing about the secret before that moment. At the same time, the other participants cannot get anything about the secret by stealing the secret value when it is transferred. However, if the dealer is lost, a certain number or more partidtmnts of them can reoonstruct the secret by ccoperating. In order to clear this concept, an illustrating scheme with geometry method and a practical scheme with algebra method is given. 展开更多
关键词 secret sharing THRESHOLD assignment scheme dynamtic assignment scheme.
在线阅读 下载PDF
Optimistic fair exchange E-commerce protocol based on secret sharing 被引量:3
2
作者 Ma Changshe Lei Feiyu Chen Kefei 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2006年第4期858-863,共6页
A key problem of electronic commerce (for short e-commerce) is fair exchange which guarantees that at the end of the transaction, either both parties involved in the trausaction receive each other's items or none d... A key problem of electronic commerce (for short e-commerce) is fair exchange which guarantees that at the end of the transaction, either both parties involved in the trausaction receive each other's items or none do. A non-interactive optimistic fair exchange e-commerce protocol model based on the publicly verifiable secret sharing is presented. The main idea of our exchange protocol is to interchange verifiable and recoverable keys of the symmetric encryption for participants' items. So it is especially suitable for exchange of large-size items. Furthermore, our protocol is efficient and simple as it does not need interactive proof system which has been adopted by a large quantity of previously proposed fair exchange protocols. Based on a modified (2,2) secret sharing scheme, a concrete non-interactive fair exchange e-commerce protocol is designed. 展开更多
关键词 E-COMMERCE optimistic fair-exchange publicly verfiable secret sharing.
在线阅读 下载PDF
Anonymous multipath routing protocol based on secret sharing in mobile ad hoc networks 被引量:2
3
作者 Siguang Chen Meng Wu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2011年第3期519-527,共9页
Because the intrinsic characteristics of mobile ad hoc networks(MANETs) cause several vulnerabilities,anonymous routing protocols attract much more attention in secure mobile ad hoc networks for the purposes of secu... Because the intrinsic characteristics of mobile ad hoc networks(MANETs) cause several vulnerabilities,anonymous routing protocols attract much more attention in secure mobile ad hoc networks for the purposes of security and privacy concerns.Until recently,lots of anonymous routing protocols have been proposed.However,most of them are single path or use one path at a time,and the multipath schemes can not thwart both the passive attacks and active attacks simultaneously.Thus an anonymous multipath routing protocol based on secret sharing is proposed.The protocol provides identity anonymity,location anonymity,data and traffic anonymity by employing cryptograph technology and secret sharing in MANET communication process.Meanwhile,a hash function is introduced to detect active attacks in the data transmission process.The protocol can effectively thwart various passive attacks and reduce the successful probability of active attacks(such as interception and physical destroy attacks).Simulation results show that the proposed scheme provides a reasonably good level of network security and performance. 展开更多
关键词 multipath routing secret sharing CRYPTOGRAPHY ANONYMITY SECURITY mobile ad hoc network(MANET).
在线阅读 下载PDF
Secret sharing scheme with inherited characteristic 被引量:1
4
作者 Ye Zhenjun Meng Fanzhen 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2006年第4期916-918,共3页
To assure the shareholders can look for their "legal" attorneys to renew the secret, once the secret sharing scheme is initialized, a secret sharing scheme with inherited characteristic is constructed. In this schem... To assure the shareholders can look for their "legal" attorneys to renew the secret, once the secret sharing scheme is initialized, a secret sharing scheme with inherited characteristic is constructed. In this scheme, each shareholder can produce a new share by his algorithm, which is equivalent to the primary one. Together with other shares, the primary secret can be renewed. Since this scheme is constructed not by replacing the primary share with a new share produced by the dealer in his primitive secret sharing scheme, so no matter how much shares the shareholder produces, these shares can not be gathered together to renew the secret in this scheme. Compared with the existing secret sharing schemes, this scheme provides more agility for the shareholders by investing each of them a function but not affect its security. 展开更多
关键词 CRYPTOGRAPHY secret sharing scheme INHERITED equivalent class.
在线阅读 下载PDF
Cheater identifiable visual secret sharing scheme 被引量:1
5
作者 GanZhi ChenKefei 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2005年第1期233-236,共4页
The visual secret sharing scheme proposed by Naor and Shamir provides a way to encrypt a secret black-white image into shares. A qualified group of participants can recover the secret message without using any cryptog... The visual secret sharing scheme proposed by Naor and Shamir provides a way to encrypt a secret black-white image into shares. A qualified group of participants can recover the secret message without using any cryptographic computation. But the original scheme can easily be corrupted by malicious participant. We propose an extension of VSS(visual secret sharing) to identify cheaters before the secret is recovered. Without the need for any additional information and cryptographic computation, every participant can verify the validity of shares of other participants, thus the security of VSS is enhanced. 展开更多
关键词 visual cryptography secret sharing VSS.
在线阅读 下载PDF
Empathetic Personification of William Thornhill in The Secret River
6
作者 Ma Lu Peng Qinglong 《学术界》 CSSCI 北大核心 2018年第10期225-236,共12页
Through a detailed text examination,this paper contends that albeit Kate Grenville'sThe Secret River is dedicated to interrogate white actions in the colonial past and expects to contribute to the process of recon... Through a detailed text examination,this paper contends that albeit Kate Grenville'sThe Secret River is dedicated to interrogate white actions in the colonial past and expects to contribute to the process of reconciliation in Australia, it engages sympathy of readers through the empathetic personification of the protagonist William Thornhill,who is subtly positioned as a victim forced into morally dubious actions by extraordinary circumstances. The wrongdoing of the white settlers is normalized in a western conception of possessive logic,the plight of the Aborigines authentically diluted and minimized. This paper thus concludes that The Secret River is another white attempt to legitimize dispossession of the Indigenous and a failure of engagement in the national reconciliation process. This paper further points out that repressing the true history will never set Australia free; acknowledging collective guilt is the only way forward. 展开更多
关键词 Empathetic PERSONIFICATION The secret RIVER SYMPATHY Kate GRENVILLE colonization DISPOSSESSION
在线阅读 下载PDF
On Grillet's Short Story “The Secret Room”——in a Phenomenological Reading Approach
7
作者 He Jian (Lanzhou Municipal Party School, Lanzhou, 730000, China) 《兰州大学学报(社会科学版)》 CSSCI 北大核心 2000年第S1期144-147,共4页
In this paper, the authortries to make a tentative analysis on the short story “The Secret Room” by Alain Robbe Grillet, a French novelist and a contemporary world famous experimentalist of “new novel” form, throu... In this paper, the authortries to make a tentative analysis on the short story “The Secret Room” by Alain Robbe Grillet, a French novelist and a contemporary world famous experimentalist of “new novel” form, through reading in phenomenological approach so as to appreciate his novel style, realize the readers esthetic imagination, meanwhile, to deepen the understanding of the process of phenomenological reading. 展开更多
关键词 The secret Room point-of-view PHENOMENOLOGICAL READING ESTHETIC IMAGINATION
在线阅读 下载PDF
Effects of Dietary Energy Level and Source on Blood Metabolites,Hormone Secretion and Follicular Fluid Composition in Gilts
8
作者 ZHOU Dong-sheng ZHUO Yong +3 位作者 WU De WANG Yan-zhong TAN Xian-yi ZHOU Ping 《畜牧兽医学报》 CAS CSCD 北大核心 2010年第S1期43-50,共8页
The objective of the study was to investigate the effects of dietary energy levels and sources on the blood metabolites,hormone secretion and the composition of follicular fluid in gilts.Fifty-four gilts with initial ... The objective of the study was to investigate the effects of dietary energy levels and sources on the blood metabolites,hormone secretion and the composition of follicular fluid in gilts.Fifty-four gilts with initial body weight of(59±4.2) kg were randomly allotted to six treatments.Treatments were low, normal,and high energy feeding levels,which were 87.5%,100%and 112.5%of recommendatory energy requirements by NRC(1998),respectively,and dietary energy sources(starch or fat).Blood samples and follicular fluids were collected on D18 and D19 of the second estrous cycle.The results showed that plasma concentrations of triglycerides and total cholesterol were higher in the fat group than that in the starch group(P【0.05),but those of glucose were similar between the two energy sources(P】0.05);dietary energy level exerted no effect on blood metabolites concentration(P】0.05).Gilts fed the high energy diet had a higher area under curve of plasma insulin(Insulin AUC),insulin-like growth factor-Ⅰ(IGF-Ⅰ) and leptin than did gilts fed the lower energy diet(P【0.05),but there was no significant difference between fat versus starch(P】0.05).Luteinizing hormone(LH) pulses were higher in gilts fed high energy rather than that in low energy diets(P】0.05),plasma concentration of estradiol(E<sub>2</sub>) was higher in the fat group than that in the starch group(P【0.05).The number of large follicles(diameter≥4 mm) and concentrations of IGF-Ⅰand E<sub>2</sub> in follicular fluid were increasing significant as the level of energy increased(P【0.05),but the numbers of large follicles and follicular fluid composition were not affected by the source of dietary energy(P】0.05).The results indicate that gilts fed high energy diets had elevated plasma concentrations of metabolic hormones,IGF-Ⅰand LH secretion,and increased follicular fluid concentrations of IGF-Ⅰ,E<sub>2</sub> and numbers of large follicles;gilts fed the dietary fat had a higher plasma concentration of cholesterol and E<sub>2</sub>. 展开更多
关键词 dietary energy levels and sources GILTS METABOLITES hormone secretion follicle fluid composition
在线阅读 下载PDF
Effect of gastric acid secretion for L-pantoprazole sodium in rats
9
《中国药理学通报》 CAS CSCD 北大核心 2015年第B11期247-248,共2页
Aim To observe the effect of L-pantoprazole sodium on gastric acid secretion in rats. Methods The rats single injection to the drug on tail after fasting but help water 48h, then threading the pyloric ligation at the ... Aim To observe the effect of L-pantoprazole sodium on gastric acid secretion in rats. Methods The rats single injection to the drug on tail after fasting but help water 48h, then threading the pyloric ligation at the junction of the pylorus and duodenum, and the animals were fasted and ban water 5h. We remove the total gastrec- tomy, gastric juice was collected and the amount of the volume, and the determination of total acidity、total acidity secretion^pH value. Results L-pantoprazole sodium and pan pantoprazole sodium can significantly reduce the to- tal acidity secretion volume (P 〈 0.05), increased gastric pH (P 〈 0.01 ). D-pantoprazole sodium on gastric acid secretion of gastric juice and total PH had no significant effect ( P 〉 0. 05 ). Conclusion L-pantoprazole sodiumhas significant inhibition of gastric acid secretion, the mechanism of the treatment of peptic ulcer may be through inhibition of proton pump discharge acid effect, to play a role in the protection of gastric mucosa. 展开更多
关键词 TOTAL GASTRIC ACID secretION PH L-pantoprazole SODIUM
在线阅读 下载PDF
Relationship Between Effect of Electro-Acupuncture on Prolactin Secretion and Several Central Neurotransmitters
10
作者 盛培林 谢启文 《中国医科大学学报》 CAS CSCD 1989年第S2期8-13,共6页
We have reported that the central mechanism of acupuncture-induced PRL secretion in non-lactating rats are related to antagonizing hypothalamic dopamine activity; noradrenaline system played little significant role in... We have reported that the central mechanism of acupuncture-induced PRL secretion in non-lactating rats are related to antagonizing hypothalamic dopamine activity; noradrenaline system played little significant role in the acupuncture effect; Υ-aminobutyric-acid system perhaps participated in this effect.This paper further provided evidence that central serotonin and EOP play a stimulatory role in the acupuncture induced secretion of prolactin; acupuncture may antagonize inhibitory effect of H<sub>2</sub> histamine receptor activation on prolactin secretion; the possible role of H<sub>1</sub>-receptor needs further investigation. 展开更多
关键词 ACUPUNCTURE ACUPUNCTURE secretion dopamine PROLACTIN PROLACTIN SEROTONIN HYPOTHALAMIC NALOXONE homeostasis
在线阅读 下载PDF
Mining the secretome of Delia antiqua(Diptera:Anthomyiidae)based on the transcriptomic and gene expression data and analysis of its potential roles in diapause development
11
作者 HAO You-Jin XU Yan-Ling CHEN Bin 《昆虫学报》 CAS CSCD 北大核心 2017年第10期1168-1177,共10页
【Aim】Insect diapause is a critical eco-physiological adaptation for seasonal survival,but little is known about the molecular mechanisms of summer and winter diapause of Delia antiqua.Here,our main goal is to identi... 【Aim】Insect diapause is a critical eco-physiological adaptation for seasonal survival,but little is known about the molecular mechanisms of summer and winter diapause of Delia antiqua.Here,our main goal is to identify candidate genes linked to pupal diapause of D.antiqua.【Methods】A straightforward in silico approach was performed to predict the secreted diapause-related proteins based on the RNA-seq and digital gene expression datasets.Amino acid sequence similarity and basic physiochemical characteristics analysis were conducted.Gene expression levels of 12 selected genes from RNA-seq data were further validated in non-diapause pupae,and different developmental stages of summer and winter diapause pupae by qRT-PCR.【Results】A total of 38 putative secreted proteins were differentially regulated between the summer and winter diapause pupae of D.antiqua.Gene Ontology analysis revealed differentially expressed genes to be associated with odorant binding,chitin and lipid metabolic processes,innate immunity and development,etc.Among them,regulation of lipid mobilization and chitin-related metabolism was found to be important during diapause development.qRT-PCR analyses showed that the expression profiles of the 12 selected genes were in good agreement with results from RNA-seq(R=0.862,P=0.001).【Conclusion】This study establishes a robust pipeline for the discovery of diapause-related genes by secretome mining based on the high-throughput sequencing. 展开更多
关键词 Delia antiqua DIAPAUSE secreted protein TRANSCRIPTOME digital gene expression RNA-seq
在线阅读 下载PDF
Proteomic mapping of secreted proteins of Trichoderma spp.
12
作者 Li S Bramley P M +1 位作者 Smith J Cannon P F 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2004年第4期455-455,共1页
A series of highly taxonomically diverse Trichoderma strains were investigated using proteomic approaches, to investigate the utility of protein profiles as taxonomic markers and to identify proteins of potential econ... A series of highly taxonomically diverse Trichoderma strains were investigated using proteomic approaches, to investigate the utility of protein profiles as taxonomic markers and to identify proteins of potential economic importance. Initial studies have focused on a comparison of single strains of T. aureoviride, T. saturnisporum, T. polysporum, T. longbrachiatum and T. spirale, along with two strains of T. harzianum. All seven strains were grown in synthetic medium supplemented with 2%(w/v) glycerol, to maximize the diversity of extracellular protein production. Samples of secreted protein were separated by 2D gel electrophoresis and will be characterized by MALDI-TOF peptide fingerprinting. 展开更多
关键词 蛋白质组学 木霉属 真菌 多肽 指纹识别
在线阅读 下载PDF
Design and realization of threshold secret sharing scheme with random weights
13
作者 Ye Zhenjun Fang Zhenming +1 位作者 Wang Chunfeng Meng Fanzhen 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2009年第5期1091-1095,共5页
A new threshold secret sharing scheme is constructed by introducing the concept of share vector, in which the number of shareholders can be adjusted by randomly changing the weights of them. This kind of scheme overco... A new threshold secret sharing scheme is constructed by introducing the concept of share vector, in which the number of shareholders can be adjusted by randomly changing the weights of them. This kind of scheme overcomes the limitation of the static weighted secret sharing schemes that cannot change the weights in the process of carrying out and the deficiency of low efficiency of the ordinary dynamic weighted sharing schemes for its resending process. Thus, this scheme is more suitable to the case that the number of shareholders needs to be changed randomly during the scheme is carrying out. 展开更多
关键词 random weight secret sharing share vector.
在线阅读 下载PDF
Whole Genome RNAi Screen in C. elegans Identifies CAB-1 as a Novel Regulator of DCV Secretion
14
作者 Xia Zhi-Ping Chen Yan +3 位作者 Sheng Yi Yi Ya-Lan Song E-Li Xu Tao 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2014年第8期787-795,共9页
关键词 摘要 编辑部 编辑工作 读者
在线阅读 下载PDF
难溶性磷对紫花苜蓿生长、根系分泌有机酸及根解剖结构的影响研究
15
作者 李亚娟 魏洁 +5 位作者 李路杰 李海燕 曹文侠 李莉娜 安彤 向文立 《中国草地学报》 北大核心 2026年第2期99-110,共12页
磷素是紫花苜蓿生长发育的重要元素,为探究紫花苜蓿利用难溶性磷的能力及其机理,以难溶性磷源[Ca_(3)(PO_(4))_(2),IP]为磷肥处理,以水溶性磷源(KH_(2)PO_(4),SP)为对照,对6个紫花苜蓿品种:‘陇东苜蓿’(M1)、‘金皇后’(M2)、‘阿尔冈... 磷素是紫花苜蓿生长发育的重要元素,为探究紫花苜蓿利用难溶性磷的能力及其机理,以难溶性磷源[Ca_(3)(PO_(4))_(2),IP]为磷肥处理,以水溶性磷源(KH_(2)PO_(4),SP)为对照,对6个紫花苜蓿品种:‘陇东苜蓿’(M1)、‘金皇后’(M2)、‘阿尔冈金’(M3)、‘中苜一号’(M4)、‘甘农9号’(M5)、‘巨能Ⅱ’(M6)进行水培试验,对其生长、植株磷吸收、根系分泌有机酸以及根系解剖结构进行研究。结果表明,难溶性磷形态下,紫花苜蓿地上部干重均低于水溶性形态,‘中苜一号’的地上部干重最高,为1.90 g,而‘甘农9号’的地上部干重最小,仅为1.00 g,‘中苜一号’在难溶性磷处理下地上部磷含量显著高于其他5个品种,为0.39%,‘陇东苜蓿’地上部磷含量最低,为0.25%。难溶性磷形态下,6种紫花苜蓿根系有机酸分泌量均高于水溶性磷形态,柠檬酸是主要的有机酸种类,‘中苜一号’根系分泌有机酸总量及柠檬酸量均显著高于其他品种。难溶性磷形态改变了紫花苜蓿根系解剖结构特征,并表现出品种差异性,‘甘农9号’具有最多的导管数目和最小的导管直径,‘陇东苜蓿’的主根直径和中柱直径最大。皮尔逊相关性分析表明紫花苜蓿地上部干重与根系苹果酸分泌量呈极显著正相关(P<0.01),与导管直径和皮层厚度呈显著正相关(P<0.05);紫花苜蓿地上部磷含量与根系分泌酒石酸量呈显著正相关(P<0.05)。灰色关联度综合分析表明‘中苜一号’‘陇东苜蓿’和‘巨能Ⅱ’在难溶性磷形态下表现良好,可作为难溶性磷资源利用以及低有效磷土壤条件下栽培紫花苜蓿的品种选择。 展开更多
关键词 紫花苜蓿 难溶性磷 生物量 磷含量 根系分泌物 根解剖结构
在线阅读 下载PDF
兼顾通信轮数与计算开销的门限多方隐私集合交集协议
16
作者 张恩 黄昱晨 +1 位作者 郑东 禹勇 《软件学报》 北大核心 2026年第4期1819-1837,共19页
(t,N)门限多方隐私集合交集协议(threshold multi-party private set intersection,TMP-PSI)允许当指定参与方的集合元素x在其余不少于t-1(t<N)个参与方的私有集合中出现时,数据元素x作为交集结果输出,在提案投票、金融交易威胁识别... (t,N)门限多方隐私集合交集协议(threshold multi-party private set intersection,TMP-PSI)允许当指定参与方的集合元素x在其余不少于t-1(t<N)个参与方的私有集合中出现时,数据元素x作为交集结果输出,在提案投票、金融交易威胁识别、安全评估等场景具有广泛应用.现有的门限多方隐私集合交集协议运行效率低、通信轮数多且只能由某一个指定参与方获取交集.针对这些问题,设计一种基于弹性秘密共享的参与方门限测试方法,结合不经意键值对存储(oblivious key-value store,OKVS)提出一种TMP-PSI方案,能够有效减少计算开销和通信轮数.为了满足多参与方获取私有集合中交集信息的需求,提出第2种拓展门限多方隐私集合交集(extended threshold multi-party private set intersection,ETMP-PSI)协议对份额分发方式进行改变,与第1种方案相比,秘密分发者和秘密重构方没有额外增加通信轮数和计算复杂度,实现了多参与方获取私有集合中的交集元素.所设计的协议在数据集合大小为n=216的三方场景下运行时间为6.4 s(TMP-PSI)和8.7 s(ETMP-PSI),与现有的门限多方隐私集合交集协议相比,重构方和分发方的通信复杂度由O(nNtlognλ)降为O(bNλ). 展开更多
关键词 门限多方隐私集合交集协议 通信轮数 计算开销 弹性秘密共享 不经意键值对存储
在线阅读 下载PDF
面向物联低压互动用户的轻量级双向认证协议
17
作者 蒋雯倩 林晓明 +4 位作者 张焜 周密 陈珏羽 钱斌 唐建林 《计算机应用与软件》 北大核心 2026年第3期91-97,124,共8页
低压用户通过智能手机与智能设备进行信息交互,满足电网侧的互动响应需求。但传统的SSL/TLS等协议对于计算能力受限的智能设备来说过于复杂,难以实现智能手机与节点设备的双向身份认证与密钥协商,导致数据泄露或篡改。基于以上问题,提... 低压用户通过智能手机与智能设备进行信息交互,满足电网侧的互动响应需求。但传统的SSL/TLS等协议对于计算能力受限的智能设备来说过于复杂,难以实现智能手机与节点设备的双向身份认证与密钥协商,导致数据泄露或篡改。基于以上问题,提出一种面向低压互动用户的轻量级双向认证通信协议,协议中引入认证中心、轻量级证书,帮助减轻智能设备的存储与计算负担,通过共享随机数实现智能手机与智能设备之间的密钥协商和双向身份认证,保证了通信安全。最后,通过BAN逻辑证明了协议的正确性。 展开更多
关键词 算力受限 双向认证 认证中心 密钥协商 BAN逻辑
在线阅读 下载PDF
复发性流产患者阴道分泌物的代谢组学分析
18
作者 庄丽怡 龚护民 +2 位作者 张培 李诗佳 黄涛 《海南医科大学学报》 北大核心 2026年第3期207-216,共10页
目的:探讨与复发性流产(recurrent spontaneous abortion,RSA)患者阴道分泌物相关的差异代谢物和代谢途径。方法:利用超高效液相色谱‑四级杆‑静电场轨道阱高分辨质谱联用(UPLC‑Q‑Exactive‑MS)技术,对63例RSA患者和47例健康女性的阴道分... 目的:探讨与复发性流产(recurrent spontaneous abortion,RSA)患者阴道分泌物相关的差异代谢物和代谢途径。方法:利用超高效液相色谱‑四级杆‑静电场轨道阱高分辨质谱联用(UPLC‑Q‑Exactive‑MS)技术,对63例RSA患者和47例健康女性的阴道分泌物进行非靶向代谢组学分析,将多元和单元统计分析相结合,筛选两者的差异代谢物,并对差异代谢物进行代谢通路分析,进而寻找代谢物与RSA的相对关系。同时通过受试者工作特征(ROC)曲线分析差异代谢物对RSA的鉴别能力。结果:在健康非孕组和健康妊娠组中筛选出L‑脯氨酸、L‑亮氨酸等8种差异代谢物和精氨酸脯氨酸代谢等4条异常代谢通路。在RSA非孕组和健康非孕组中筛选出2,3‑Dinor‑8‑表‑前列腺素F2α、3‑(2‑萘基)‑L‑丙氨酸等13种差异代谢物和嘧啶代谢等2条异常代谢通路。在RSA妊娠组和健康妊娠组中筛选出甘氨熊脱氧胆酸、D‑(‑)‑核糖等7种差异代谢物和磷酸戊糖途径等2条异常代谢通路。通过ROC曲线分析确定了曲线下面积(AUC)>0.7的差异性代谢物3种,包括2,3‑Dinor‑8‑表‑前列腺素F2α、3‑(2‑萘基)‑L‑丙氨酸、甘氨熊脱氧胆酸。结论:RSA患者与健康妇女的阴道分泌物代谢谱存在差异,2,3‑Dinor‑8‑表‑前列腺素F2α、3‑(2‑萘基)‑L‑丙氨酸、甘氨熊脱氧胆酸这3种代谢物对RSA表现了出色的判别效能,有望作为潜在的生物标志物。 展开更多
关键词 复发性流产 代谢组学 阴道分泌物 液相色谱‑质谱联用
在线阅读 下载PDF
犬瘟热病毒YT1404D株血凝素蛋白在昆虫细胞中的表达及鉴定
19
作者 李少祖 张涛 +6 位作者 李梦丽 李璐 周雨涵 杨子茹月 何金娇 李鹏 孙国鹏 《动物医学进展》 北大核心 2026年第2期14-20,共7页
为获取具有良好抗原性且接近天然构象的犬瘟热病毒(Canine distemper virus,CDV)全长血凝素蛋白(Hemagglutinin,H),对YT1404D毒株H基因进行人工修饰,设计带有编码gp67分泌信号肽和纯化标签的GP67-H-His_(tag)融合基因,经优化密码子后合... 为获取具有良好抗原性且接近天然构象的犬瘟热病毒(Canine distemper virus,CDV)全长血凝素蛋白(Hemagglutinin,H),对YT1404D毒株H基因进行人工修饰,设计带有编码gp67分泌信号肽和纯化标签的GP67-H-His_(tag)融合基因,经优化密码子后合成用于构建pFastBac-GP67-H-His_(tag)重组质粒,将其转化至DH10 Bac感受态细胞获得重组杆粒rBacmid-GP67-H-His_(tag)。重组杆粒转染Sf9细胞制备重组杆状病毒rBAC-rH,并纯化制备重组H融合蛋白,用Western blot对该重组蛋白进行反应原性鉴定。结果显示,H融合蛋白分泌表达于细胞培养上清中,分子质量约70 ku,纯化后产率可达2.6 mg/100 mL。Western blot鉴定该融合蛋白分别与CDV阳性血清和疫苗株免疫鼠的阳性血清发生特异性反应,表明该融合蛋白具有良好的反应原性。为深入研究H蛋白N-糖基化差异对其免疫和反应原性的影响,开发地域流行特点的CDV亚单位疫苗奠定了基础。 展开更多
关键词 犬瘟热病毒 血凝素蛋白 杆状病毒-昆虫细胞表达系统 分泌表达
在线阅读 下载PDF
低能量激光联合维生素治疗灼口综合征的效果及对唾液AMS、MMP-9、SIgA水平的影响
20
作者 尹俊 李静 +2 位作者 刘琳 李岳 黄涛 《实用医学杂志》 北大核心 2026年第4期595-602,共8页
目的 分析灼口综合征接受低能量激光联合维生素治疗的效果及对唾液淀粉酶(AMS)、基质金属蛋白酶-9(MMP-9)、分泌型免疫球蛋白A(SIgA)水平的影响。方法 通过随机数字表法将2023年11月至2025年4月衡水市人民医院收治的灼口综合征患者174... 目的 分析灼口综合征接受低能量激光联合维生素治疗的效果及对唾液淀粉酶(AMS)、基质金属蛋白酶-9(MMP-9)、分泌型免疫球蛋白A(SIgA)水平的影响。方法 通过随机数字表法将2023年11月至2025年4月衡水市人民医院收治的灼口综合征患者174例分为维生素组(n=58)、低能量激光组(n=58)、联合组(n=58)。维生素组口服维生素E 100 mg+谷维素10 mg+维生素B_(2) 10 mg治疗,低能量激光组在接受低能量激光治疗、联合组接受口服维生素结合低能量激光治疗。10 d为1个疗程,3组患者共治疗2个疗程。比较3组治疗2个疗程后疗效,治疗前、治疗2个疗程后唾液分泌、疼痛、唾液AMS、MMP-9、SIgA水平、血清脑源性神经营养因子(BDNF)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平、焦虑、抑郁及睡眠评分,治疗期间安全性。结果 联合组总有效率为94.83%,高于维生素组的75.86%、低能量激光组的79.31%(P<0.05)。相较治疗前,治疗2个疗程后3组静息状态下唾液流量、刺激状态下唾液流量、唾液AMS、SIgA水平、血清BDNF水平升高,且联合组更高(P<0.05)。相较治疗前,治疗2个疗程后3组视觉模拟评分(VAS)评分、唾液MMP-9水平、血清IL-6、TNF-α水平,广泛性焦虑量表(GAD-7)、健康问卷抑郁量表(PHQ-9)、匹兹堡睡眠质量指数量表(PSQI)评分降低,且联合组更低(P<0.05)。3组治疗期间均无严重不良反应报告,均完成全程治疗。结论 在口服维生素的基础上,应用低能量激光可促进灼口综合征患者唾液分泌,降低疼痛,改善焦虑、抑郁情况及睡眠质量,调节唾液AMS、MMP-9、SIgA及血清BDNF、IL-6、TNF-α水平,进而改善口腔功能并降低神经炎症,安全性良好。 展开更多
关键词 灼口综合征 低能量激光 维生素 唾液分泌 淀粉酶 基质金属蛋白酶-9 分泌型免疫球蛋白A 疼痛
在线阅读 下载PDF
上一页 1 2 168 下一页 到第
使用帮助 返回顶部