以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上...以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上升。在汁胞粒化发生过程中,汁胞维持较低的细胞壁降解酶[果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、纤维素酶(Cx)]活性,保持较高的细胞壁物质(原果胶、纤维素、半纤维素)含量;尤其在汁胞粒化的起动阶段和加快阶段,纤维素、半纤维素含量极显著增加。相反,适龄树果实的汁胞粒化指数在果实成熟过程中变化不大,汁胞中细胞壁降解酶活性较高,促进原果胶、纤维素、半纤维素等细胞壁物质的降解,保持较低的细胞壁物质含量,使汁胞发育正常、柔软多汁。这说明PE、PG、Cx活性和原果胶、纤维素、半纤维素含量与琯溪蜜柚汁胞粒化密切相关。展开更多
To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum ...To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.展开更多
文摘以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上升。在汁胞粒化发生过程中,汁胞维持较低的细胞壁降解酶[果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、纤维素酶(Cx)]活性,保持较高的细胞壁物质(原果胶、纤维素、半纤维素)含量;尤其在汁胞粒化的起动阶段和加快阶段,纤维素、半纤维素含量极显著增加。相反,适龄树果实的汁胞粒化指数在果实成熟过程中变化不大,汁胞中细胞壁降解酶活性较高,促进原果胶、纤维素、半纤维素等细胞壁物质的降解,保持较低的细胞壁物质含量,使汁胞发育正常、柔软多汁。这说明PE、PG、Cx活性和原果胶、纤维素、半纤维素含量与琯溪蜜柚汁胞粒化密切相关。
文摘To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.
