Affinity propagation(AP)is a classic clustering algorithm.To improve the classical AP algorithms,we propose a clustering algorithm namely,adaptive spectral affinity propagation(AdaSAP).In particular,we discuss why AP ...Affinity propagation(AP)is a classic clustering algorithm.To improve the classical AP algorithms,we propose a clustering algorithm namely,adaptive spectral affinity propagation(AdaSAP).In particular,we discuss why AP is not suitable for non-spherical clusters and present a unifying view of nine famous arbitrary-shaped clustering algorithms.We propose a strategy of extending AP in non-spherical clustering by constructing category similarity of objects.Leveraging the monotonicity that the clusters’number increases with the self-similarity in AP,we propose a model selection procedure that can determine the number of clusters adaptively.For the parameters introduced by extending AP in non-spherical clustering,we provide a grid-evolving strategy to optimize them automatically.The effectiveness of AdaSAP is evaluated by experiments on both synthetic datasets and real-world clustering tasks.Experimental results validate that the superiority of AdaSAP over benchmark algorithms like the classical AP and spectral clustering algorithms.展开更多
Leaves of a Metasequoia glyptostroboides tree in Paomu Village,Longshan County,Hunan Province (Paomu Tree) possess"Even Type"cuticles and frill-margined stomata similar to those of fossil Metasequoia by far ...Leaves of a Metasequoia glyptostroboides tree in Paomu Village,Longshan County,Hunan Province (Paomu Tree) possess"Even Type"cuticles and frill-margined stomata similar to those of fossil Metasequoia by far known but different from all other living Metasequoia trees(common trees).Paomu Tree is thus hypothesized as the real"living fossil",which has been supported by preliminary DNA analyses. Techniques of clearing leaf,epidermis stripping,展开更多
A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates,nucleic acid components,glycoproteins,and other small biomolecules.The mechanisms of...A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates,nucleic acid components,glycoproteins,and other small biomolecules.The mechanisms of interactions between boronate ligands and analytes are described.Various boronate ligands and supports are discussed.Examples of the use of boronate affinity chromatography for separation of each class of analytes are presented.展开更多
In this paper a negative electron affinity （NEA） multialkali photocathode of （Na<sub>2</sub>KSb-Cs）-O-Cs structure is fabricated by new technology. It is found that its emission stability is much bette...In this paper a negative electron affinity （NEA） multialkali photocathode of （Na<sub>2</sub>KSb-Cs）-O-Cs structure is fabricated by new technology. It is found that its emission stability is much better than that of the NEA GaAs photocathode, but is inferior to that of the conventional Na<sub>2</sub>KSb（Cs）. After 70 hour performance in a pumping vacuum system, the emission sensitivity of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs photocathode drops only by 2.5%. The emission stability is closely related to the states of the activation cesium and oxygen during activation, best results being obtained with cesium ions and excited oxygen. Furthermore, better photoemission sensitivity and emission stability may be obtained if the cathode is illuminated by intense white light during the activation process. The performance of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs cathode which has not been illuminated by intense white light during activation may be improved by the illumination even during operation intermission.展开更多
Integrins are heterodimers that mediate cell adhesion and transduce signals bidirectionally across the cell membrane.Integrins often exist in low affinity(or inactive) states for
芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛...芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛选法(磁珠-SELEX)开展10轮筛选,经由高通量测序获得6条候补序列(S1~S6),并进行家族性、同源性分析及二级结构预测。结果表明,6条候选核酸适体的重复率可达46.38%,其自由能在-9.02到-2.47 kcal·moL^(-1)之间,根据自由能能量稳定原则,S1和S5吉布斯自由能最低最稳定,分别为-6.70和-9.02 kcal·moL^(-1)。利用ELISA试验进行亲和力测试,结果表明核酸适体S1和S2的亲和能力较强,S1:KD=67.02 nmol·L^(-1),R2=0.925 8,S2:KD=97.65 nmol·L^(-1),R2=0.795 1。核酸适体S1与过敏原Ses i 2的结合力和其他过敏原蛋白相比有显著差异,可视为具有特异性。本研究最终获得一条兼具良好亲和力和特异性的核酸适体S1,为芝麻过敏原快速检测提供了技术支撑。展开更多
基金This work was supported by the National Natural Science Foundation of China(71771034,71901011,71971039)the Scientific and Technological Innovation Foundation of Dalian(2018J11CY009).
文摘Affinity propagation(AP)is a classic clustering algorithm.To improve the classical AP algorithms,we propose a clustering algorithm namely,adaptive spectral affinity propagation(AdaSAP).In particular,we discuss why AP is not suitable for non-spherical clusters and present a unifying view of nine famous arbitrary-shaped clustering algorithms.We propose a strategy of extending AP in non-spherical clustering by constructing category similarity of objects.Leveraging the monotonicity that the clusters’number increases with the self-similarity in AP,we propose a model selection procedure that can determine the number of clusters adaptively.For the parameters introduced by extending AP in non-spherical clustering,we provide a grid-evolving strategy to optimize them automatically.The effectiveness of AdaSAP is evaluated by experiments on both synthetic datasets and real-world clustering tasks.Experimental results validate that the superiority of AdaSAP over benchmark algorithms like the classical AP and spectral clustering algorithms.
文摘Leaves of a Metasequoia glyptostroboides tree in Paomu Village,Longshan County,Hunan Province (Paomu Tree) possess"Even Type"cuticles and frill-margined stomata similar to those of fossil Metasequoia by far known but different from all other living Metasequoia trees(common trees).Paomu Tree is thus hypothesized as the real"living fossil",which has been supported by preliminary DNA analyses. Techniques of clearing leaf,epidermis stripping,
文摘A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates,nucleic acid components,glycoproteins,and other small biomolecules.The mechanisms of interactions between boronate ligands and analytes are described.Various boronate ligands and supports are discussed.Examples of the use of boronate affinity chromatography for separation of each class of analytes are presented.
基金This work is supported by the Natural Science Foundation of Fujian Province, China.
文摘In this paper a negative electron affinity （NEA） multialkali photocathode of （Na<sub>2</sub>KSb-Cs）-O-Cs structure is fabricated by new technology. It is found that its emission stability is much better than that of the NEA GaAs photocathode, but is inferior to that of the conventional Na<sub>2</sub>KSb（Cs）. After 70 hour performance in a pumping vacuum system, the emission sensitivity of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs photocathode drops only by 2.5%. The emission stability is closely related to the states of the activation cesium and oxygen during activation, best results being obtained with cesium ions and excited oxygen. Furthermore, better photoemission sensitivity and emission stability may be obtained if the cathode is illuminated by intense white light during the activation process. The performance of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs cathode which has not been illuminated by intense white light during activation may be improved by the illumination even during operation intermission.
文摘Integrins are heterodimers that mediate cell adhesion and transduce signals bidirectionally across the cell membrane.Integrins often exist in low affinity(or inactive) states for
文摘芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛选法(磁珠-SELEX)开展10轮筛选,经由高通量测序获得6条候补序列(S1~S6),并进行家族性、同源性分析及二级结构预测。结果表明,6条候选核酸适体的重复率可达46.38%,其自由能在-9.02到-2.47 kcal·moL^(-1)之间,根据自由能能量稳定原则,S1和S5吉布斯自由能最低最稳定,分别为-6.70和-9.02 kcal·moL^(-1)。利用ELISA试验进行亲和力测试,结果表明核酸适体S1和S2的亲和能力较强,S1:KD=67.02 nmol·L^(-1),R2=0.925 8,S2:KD=97.65 nmol·L^(-1),R2=0.795 1。核酸适体S1与过敏原Ses i 2的结合力和其他过敏原蛋白相比有显著差异,可视为具有特异性。本研究最终获得一条兼具良好亲和力和特异性的核酸适体S1,为芝麻过敏原快速检测提供了技术支撑。
