Paclitaxel in southern yew tree was quantitatively determined by high performance liquid chromatography (HPLC) with ODS-C18 column. A mixture of CH3OH-H2O-CH3COOH(volume ratio: 55 :44 :1) is used as mobile phase and U...Paclitaxel in southern yew tree was quantitatively determined by high performance liquid chromatography (HPLC) with ODS-C18 column. A mixture of CH3OH-H2O-CH3COOH(volume ratio: 55 :44 :1) is used as mobile phase and UV detection is carried out at 227 nm, and the column temperature is 20℃. The results show that there is a good linear felationship between the area of paclitaxel and the concentration of the sample in the range 50-500 mg/L for paclitaxel. The corresponding regression equation is Y= 13 021. 7 + 1. 01 × 106X, r=0. 9990. The average recovery is 95. 3% and the relative standard deviation is 2. 08%.展开更多
基金Project (00KNY1010 ) supported by the Key Program of Science and Technology Office of Hunan Province
文摘Paclitaxel in southern yew tree was quantitatively determined by high performance liquid chromatography (HPLC) with ODS-C18 column. A mixture of CH3OH-H2O-CH3COOH(volume ratio: 55 :44 :1) is used as mobile phase and UV detection is carried out at 227 nm, and the column temperature is 20℃. The results show that there is a good linear felationship between the area of paclitaxel and the concentration of the sample in the range 50-500 mg/L for paclitaxel. The corresponding regression equation is Y= 13 021. 7 + 1. 01 × 106X, r=0. 9990. The average recovery is 95. 3% and the relative standard deviation is 2. 08%.
