Many existing studies have considered the factors influencing review helpfulness,mainly focusing on reviewer impact,review informativeness,and managerial response,based on signaling theory.However,previous studies hav...Many existing studies have considered the factors influencing review helpfulness,mainly focusing on reviewer impact,review informativeness,and managerial response,based on signaling theory.However,previous studies have simply regarded these factors as independent signals,thus ignoring their in-depth transmission and reception processes.The conclusions about the impact of reviewers on review helpfulness are also inconsistent due to the inaccurate measure-ment of variables.To fill the above gaps,we followed the signaling timeline theoretical framework used in signaling the-ory and employed a bootstrapping analysis to examine how reviewer impact,review informativeness,and hotel manageri-al responses interact to influence review helpfulness.In this study,we used a unique dataset that included official labels from one leading online travel agency.The results show that reviewer impact may affect review helpfulness sequentially through review informativeness and hotel managerial response.Furthermore,by using official labels,both reviewer expert-ise and reviewer experience significantly affect review helpfulness.Finally,we discussed the theoretical and practical im-plications of these findings.展开更多
人Elp3(human elongator protein 3,hElp3)具有组蛋白乙酰转移酶活性,是与延伸中的RNA聚合酶Ⅱ结合的elongator复合物的催化亚基,可参与组蛋白的乙酰化修饰与基因的转录延伸.Elp3及其复合物功能异常与人类多种疾病相关.为运用染色质免...人Elp3(human elongator protein 3,hElp3)具有组蛋白乙酰转移酶活性,是与延伸中的RNA聚合酶Ⅱ结合的elongator复合物的催化亚基,可参与组蛋白的乙酰化修饰与基因的转录延伸.Elp3及其复合物功能异常与人类多种疾病相关.为运用染色质免疫沉淀等手段深入研究Elp3功能,PCR法克隆pYES2-hElp3质粒中编码hElp3的N端亲水区段(1～69氨基酸残基),构建原核表达载体pMXB10-hElp3-210,经IPTG诱导和几丁质柱纯化后,免疫兔制备多克隆抗体.ELISA检测显示,该抗体有较高的效价(不低于1∶2 500).免疫印迹实验结果表明,该抗体可与纯化的及HeLa细胞中的hElp3蛋白特异性结合.运用该抗体对转入elp3Δ菌株的人Elp3的染色质免疫沉淀实验结果表明,人Elp3可参与酵母SSA3基因的转录调控,这可能是人Elp3能够部分补偿酵母SSA3基因延迟表达缺陷的原因.展开更多
TESCO作为英国最大零售商、世界连锁超市经营第三大零售商,其竞争优势主要表现在:自有品牌"Every little helps"物有所值商品优势、互联网服务优势和数据库分析优势。2004年TESCO低调进入中国市场。经过10年的发展,TESCO"...TESCO作为英国最大零售商、世界连锁超市经营第三大零售商,其竞争优势主要表现在:自有品牌"Every little helps"物有所值商品优势、互联网服务优势和数据库分析优势。2004年TESCO低调进入中国市场。经过10年的发展,TESCO"乐购"超市门店数量从最初的25家发展到135家。2013年,由于TESCO公司利润下降,迫使TESCO公司转让在华的135个大卖场给华润万家。导致TESCO乐购淡出中国连锁超市零售业的主要原因有三个:一是TESCO没有形成规模经济;二是运作模式单一;三是本土化缺失。值得一提的是,TESCO淡出中国超市零售业不存在"败走麦城"的现象,华润万家与TESCO乐购的合作是"融合"不是并购,双方以80:20的比例成立合资公司,这也是TESCO海外拓展的主要方式之一。展开更多
基金supported by the Philosophy and Social Science Planning Program of Shanghai(2021BGL018).
文摘Many existing studies have considered the factors influencing review helpfulness,mainly focusing on reviewer impact,review informativeness,and managerial response,based on signaling theory.However,previous studies have simply regarded these factors as independent signals,thus ignoring their in-depth transmission and reception processes.The conclusions about the impact of reviewers on review helpfulness are also inconsistent due to the inaccurate measure-ment of variables.To fill the above gaps,we followed the signaling timeline theoretical framework used in signaling the-ory and employed a bootstrapping analysis to examine how reviewer impact,review informativeness,and hotel manageri-al responses interact to influence review helpfulness.In this study,we used a unique dataset that included official labels from one leading online travel agency.The results show that reviewer impact may affect review helpfulness sequentially through review informativeness and hotel managerial response.Furthermore,by using official labels,both reviewer expert-ise and reviewer experience significantly affect review helpfulness.Finally,we discussed the theoretical and practical im-plications of these findings.
文摘人Elp3(human elongator protein 3,hElp3)具有组蛋白乙酰转移酶活性,是与延伸中的RNA聚合酶Ⅱ结合的elongator复合物的催化亚基,可参与组蛋白的乙酰化修饰与基因的转录延伸.Elp3及其复合物功能异常与人类多种疾病相关.为运用染色质免疫沉淀等手段深入研究Elp3功能,PCR法克隆pYES2-hElp3质粒中编码hElp3的N端亲水区段(1～69氨基酸残基),构建原核表达载体pMXB10-hElp3-210,经IPTG诱导和几丁质柱纯化后,免疫兔制备多克隆抗体.ELISA检测显示,该抗体有较高的效价(不低于1∶2 500).免疫印迹实验结果表明,该抗体可与纯化的及HeLa细胞中的hElp3蛋白特异性结合.运用该抗体对转入elp3Δ菌株的人Elp3的染色质免疫沉淀实验结果表明,人Elp3可参与酵母SSA3基因的转录调控,这可能是人Elp3能够部分补偿酵母SSA3基因延迟表达缺陷的原因.
文摘TESCO作为英国最大零售商、世界连锁超市经营第三大零售商,其竞争优势主要表现在:自有品牌"Every little helps"物有所值商品优势、互联网服务优势和数据库分析优势。2004年TESCO低调进入中国市场。经过10年的发展,TESCO"乐购"超市门店数量从最初的25家发展到135家。2013年,由于TESCO公司利润下降,迫使TESCO公司转让在华的135个大卖场给华润万家。导致TESCO乐购淡出中国连锁超市零售业的主要原因有三个:一是TESCO没有形成规模经济;二是运作模式单一;三是本土化缺失。值得一提的是,TESCO淡出中国超市零售业不存在"败走麦城"的现象,华润万家与TESCO乐购的合作是"融合"不是并购,双方以80:20的比例成立合资公司,这也是TESCO海外拓展的主要方式之一。
