Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity ...Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.展开更多
采用文献资料等方法对"Exercise is Medicine"领域现状进行了研究及探讨其对我国健康事业发展的启示。研究结果显示,EIM项目已在美国逐步兴起、发展并得到社会大众的认可,形成了比较完整的知识应用体系。目前,EIM项目主要应...采用文献资料等方法对"Exercise is Medicine"领域现状进行了研究及探讨其对我国健康事业发展的启示。研究结果显示,EIM项目已在美国逐步兴起、发展并得到社会大众的认可,形成了比较完整的知识应用体系。目前,EIM项目主要应用于心肺耐力、心血管疾病、骨质疏松、内分泌功能紊乱、消化系统等与静坐不运动密切关联身体疾病。对我国主要的启示:引进EIM项目并推广;构建符合我国国情的EIM体系;构建我国EIM从业资格和职业认证体系;引进社会资源,推动EIM快速发展;政府给予绿色通道,政策给予保障。展开更多
To make rat do active exercise to counteract bone loss in the rat tail-suspension model, a pulse electrical stimulator based on single-electrode with a low-current and a high-voltage was designed. The stimulator was c...To make rat do active exercise to counteract bone loss in the rat tail-suspension model, a pulse electrical stimulator based on single-electrode with a low-current and a high-voltage was designed. The stimulator was controlled by SCM (single chip micyoco) that could accurately control the stimulation duration and the interval between stimulations, and cease the operation after the recorded number of stimulation had reached the value set by the program. With the help of posture estimation part, the device would operate intelligently by determining whether to stimulate or not, depending on the posture of rat's limb. Software was developed to make operator control the stimulator using computer, save the experiment data and print the report. In practical experiment, the voltaic arc is generated by the stimulator, and impacted on the rat's thenar. This induced pain to the rat and the rat would actively contract its hindlimb to evade the pain, so active exercise was carried out. The tail-suspension rats were trained twice every day for 14 d. At the 0 and 14th day, bone mineral density of rat femurs was determined by dual energy X-ray absorptiometry (DXA). The results show that the active exercise stimulated by the pulse electrical arc stimulator can attenuate weightlessness-induced bone loss, and this device is a convenient steady performance electrical stimulator that can surely induce rat's hindlimb to do active exercise.展开更多
Objective Cardiopulmonary exercise testing(CPET)is helpful to identify right ventriclar(RV)dysfunction in patients with rapair of Tetralogy of Fallot(rTOF),but its predictive value on early outcomes of pulmonary valve...Objective Cardiopulmonary exercise testing(CPET)is helpful to identify right ventriclar(RV)dysfunction in patients with rapair of Tetralogy of Fallot(rTOF),but its predictive value on early outcomes of pulmonary valve replacement(PVR)of these patients is unclear when similar preoperative ventricular size and function in cardiovascular magnetic resonance(CMR)exist.The aim of this study is to evaluate whether CPET is useful to predict the early outcomes of rTOF patients after PVR.展开更多
成年人中60%—80%有下背痛（1ow back pain,LBP）患病史,美国统计资料显示,其发病率仅次于上呼吸道感染,列第2位[1]。50%以上的下背痛初次发病4—8周内可自愈,但复发率达85%[2]。LBP中约85%原因不明,称为非特异性下背痛（nonspecific lo...成年人中60%—80%有下背痛（1ow back pain,LBP）患病史,美国统计资料显示,其发病率仅次于上呼吸道感染,列第2位[1]。50%以上的下背痛初次发病4—8周内可自愈,但复发率达85%[2]。LBP中约85%原因不明,称为非特异性下背痛（nonspecific low back pain,NLBP）,其病因复杂,治疗方法虽多,但并无特效方法且治愈后复发率高,故如何提高NLBP患者疗效及降低复发率具有重要临床意义。展开更多
Aim To simulate the condition when people travel to the plateau. To make a primary investigation about the mice models, which are respectively provoked by exhaustive exercise, cold and fatigue, and epinephrine. Method...Aim To simulate the condition when people travel to the plateau. To make a primary investigation about the mice models, which are respectively provoked by exhaustive exercise, cold and fatigue, and epinephrine. Methods Divide the Kunming mice into 8 groups in random, with 10 in a single group, control group in plain; exhaustive exercise group in plain; fatigue ＆ cold group in plain; epinephrine stimulating group in plain; control group in plateau; exhaustive exercise group in plateau; fatigue ＆ cold group in plateau; epinephrine stimulating group in plateau. To simulate the condition when people travel to Lhasa whose altitude reaches 3700m, those mice were carried there during a five-day period. Day 1： Weight the mice after fasting for 12hrs, then treated different with corresponding methods. Day 2： Collect the data of mortality in each group and sacrifice the alive. Weight the cardiac, pulmonary and cerebral tissues and calculate for the Viscera Index. Results After the journey to the plat- eau, the weight of mice decreases significantly, in exhaustive exercise group and fatigue ＆ cold group also with the increase of pulmonary and cerebral index and decrease of cardiac index compared with groups in plain. As for those who are stimulated with epinephrine, the ones in plateau suffer more from pneumonedema but have a longer life span. The sensitivity to epinephrine can decrease in female mice in plateau, which can be reversed in plain. Con- clusions After the journey to plateau, acute plateau pneumonedema and cerebral edema can be provoked by ex- haustive exercise, fatigue and cold, and starvation; The severity of pneumonedema caused by epinephrine are relat- ed to the environment, strength and gender.展开更多
940184 鲤鱼稳态运动和水酸化之间存在协同作用吗?=Is there a synergistic effect be-tween steady-state exercise and water acidifica-tion in carp?[刊,英]/Dijk P V,Thillart G VD,Bonga S W//J.Fish Biol..—1993,42(5).
文摘Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.
文摘采用文献资料等方法对"Exercise is Medicine"领域现状进行了研究及探讨其对我国健康事业发展的启示。研究结果显示,EIM项目已在美国逐步兴起、发展并得到社会大众的认可,形成了比较完整的知识应用体系。目前,EIM项目主要应用于心肺耐力、心血管疾病、骨质疏松、内分泌功能紊乱、消化系统等与静坐不运动密切关联身体疾病。对我国主要的启示:引进EIM项目并推广;构建符合我国国情的EIM体系;构建我国EIM从业资格和职业认证体系;引进社会资源,推动EIM快速发展;政府给予绿色通道,政策给予保障。
基金Project(10872024) supported by the National Natural Science Foundation of China
文摘To make rat do active exercise to counteract bone loss in the rat tail-suspension model, a pulse electrical stimulator based on single-electrode with a low-current and a high-voltage was designed. The stimulator was controlled by SCM (single chip micyoco) that could accurately control the stimulation duration and the interval between stimulations, and cease the operation after the recorded number of stimulation had reached the value set by the program. With the help of posture estimation part, the device would operate intelligently by determining whether to stimulate or not, depending on the posture of rat's limb. Software was developed to make operator control the stimulator using computer, save the experiment data and print the report. In practical experiment, the voltaic arc is generated by the stimulator, and impacted on the rat's thenar. This induced pain to the rat and the rat would actively contract its hindlimb to evade the pain, so active exercise was carried out. The tail-suspension rats were trained twice every day for 14 d. At the 0 and 14th day, bone mineral density of rat femurs was determined by dual energy X-ray absorptiometry (DXA). The results show that the active exercise stimulated by the pulse electrical arc stimulator can attenuate weightlessness-induced bone loss, and this device is a convenient steady performance electrical stimulator that can surely induce rat's hindlimb to do active exercise.
文摘Objective Cardiopulmonary exercise testing(CPET)is helpful to identify right ventriclar(RV)dysfunction in patients with rapair of Tetralogy of Fallot(rTOF),but its predictive value on early outcomes of pulmonary valve replacement(PVR)of these patients is unclear when similar preoperative ventricular size and function in cardiovascular magnetic resonance(CMR)exist.The aim of this study is to evaluate whether CPET is useful to predict the early outcomes of rTOF patients after PVR.
文摘成年人中60%—80%有下背痛（1ow back pain,LBP）患病史,美国统计资料显示,其发病率仅次于上呼吸道感染,列第2位[1]。50%以上的下背痛初次发病4—8周内可自愈,但复发率达85%[2]。LBP中约85%原因不明,称为非特异性下背痛（nonspecific low back pain,NLBP）,其病因复杂,治疗方法虽多,但并无特效方法且治愈后复发率高,故如何提高NLBP患者疗效及降低复发率具有重要临床意义。
文摘Aim To simulate the condition when people travel to the plateau. To make a primary investigation about the mice models, which are respectively provoked by exhaustive exercise, cold and fatigue, and epinephrine. Methods Divide the Kunming mice into 8 groups in random, with 10 in a single group, control group in plain; exhaustive exercise group in plain; fatigue ＆ cold group in plain; epinephrine stimulating group in plain; control group in plateau; exhaustive exercise group in plateau; fatigue ＆ cold group in plateau; epinephrine stimulating group in plateau. To simulate the condition when people travel to Lhasa whose altitude reaches 3700m, those mice were carried there during a five-day period. Day 1： Weight the mice after fasting for 12hrs, then treated different with corresponding methods. Day 2： Collect the data of mortality in each group and sacrifice the alive. Weight the cardiac, pulmonary and cerebral tissues and calculate for the Viscera Index. Results After the journey to the plat- eau, the weight of mice decreases significantly, in exhaustive exercise group and fatigue ＆ cold group also with the increase of pulmonary and cerebral index and decrease of cardiac index compared with groups in plain. As for those who are stimulated with epinephrine, the ones in plateau suffer more from pneumonedema but have a longer life span. The sensitivity to epinephrine can decrease in female mice in plateau, which can be reversed in plain. Con- clusions After the journey to plateau, acute plateau pneumonedema and cerebral edema can be provoked by ex- haustive exercise, fatigue and cold, and starvation; The severity of pneumonedema caused by epinephrine are relat- ed to the environment, strength and gender.
文摘940184 鲤鱼稳态运动和水酸化之间存在协同作用吗?=Is there a synergistic effect be-tween steady-state exercise and water acidifica-tion in carp?[刊,英]/Dijk P V,Thillart G VD,Bonga S W//J.Fish Biol..—1993,42(5).
