Suid is one of the most common taxa among the Pleistocene mammalian fauna in China.However,its phylogenetic taxonomy and evolution are far from clear.The newly recovered suid fossil materials from the Early Pleistocen...Suid is one of the most common taxa among the Pleistocene mammalian fauna in China.However,its phylogenetic taxonomy and evolution are far from clear.The newly recovered suid fossil materials from the Early Pleistocene Shanshenmiaozui site in Nihewan Basin in North China provide new insight into the evolution of its kind in North China.The new materials include partial skulls and mandibles of males,and most of their teeth were preserved in situ.The specimens can be referred to the species Sus lydekkeri,which is the only species of Sus found in North China from the Early Pleistocene.The fossils of the once reported Pleistocene species“Potamochoerus chinhsienensis”should also be provisionally included in the species S.lydekkeri according to its form and size,which at least now excludes it from Potamochoerus.The species S.lydekkeri is very close to the extant species S.scrofa,and even was combined into the latter as a chronological subspecies by some authors,while its larger size,prominent preorbital fossa,backwardly positioned infraorbital foramen,frequently appearing verrucosic type(at least the intermediate type of the male lower canine),and simple crown structures support its validness as an independent species.The Early Pleistocene forms are characterized by a larger size,lower L/W ratio of cheekteeth,both upper and lower M3s having no more than three lobes,and the male lower canine is exclusively of a verrucosic type.Suid fossils are a very good indicator of paleoenvironments and paleoclimate.Both the northern and the southern faunas in China contain suid fossils throughout the Pleistocene Epoch,while the southern fauna has prominently more diversified taxa and more abundant fossil collections;whether that is caused by different origins or by environmental modifications is still not clear.展开更多
The micromammal fossils collected from the Shangzhuang Formation of the Linxia Basin,Gansu,are described here.The assemblage consists of 16 species of 13 genera belonging to 7 families,including one new species and on...The micromammal fossils collected from the Shangzhuang Formation of the Linxia Basin,Gansu,are described here.The assemblage consists of 16 species of 13 genera belonging to 7 families,including one new species and one taxon previously unknown from China.Among them 5 genera are Oligocene holdovers and 11 genera/species are known to make their first appearances in Early Miocene,indicating an Early Miocene age for the Gucheng Fauna.The absence of Oligocene survivors such as Tataromys,Yindirtemys,Eucricetodon and Tachyoryctoides,which usually occur in the Xiejian age of Early Miocene,and the presence of newcomers Protalactaga,Megacricetodon and Gobicricetodon,that made their first appearance only in the Shanwangian age of late Early Miocene,suggest that the Gucheng Fauna is of a later age of Early Miocene.The new species,Sinolagomys guchengensis,showing more derived morphology than in S.ulunguensis,seems to support an assessment of the fauna in Shanwangian age.Thus,the Gucheng Fauna may belong to late Early Miocene Shanwangian,partially equivalent to MN 3/4 of the European land mammal zonation.Ecological analysis of the fauna indicates that the palaeoecological condition of the Gucheng area might still be a predominantly arid shrub-steppe in late Early Miocene.展开更多
Parkinson’s disease(PD)is a common neurodegenerative disorder with profound impact on patients’quality of life and long-term health,and early detection and intervention are particularly critical.In recent years,the ...Parkinson’s disease(PD)is a common neurodegenerative disorder with profound impact on patients’quality of life and long-term health,and early detection and intervention are particularly critical.In recent years,the search for precise and reliable biomarkers has become one of the key strategies to effectively address the clinical challenges of PD.In this paper,we systematically evaluated potential biomarkers,including proteins,metabolites,epigenetic markers,and exosomes,in the peripheral blood of PD patients.Protein markers are one of the main directions of biomarker research in PD.In particular,α‑synuclein and its phosphorylated form play a key role in the pathological process of PD.It has been shown that aggregation ofα-synuclein may be associated with pathologic protein deposition in PD and may be a potential marker for early diagnosis of PD.In terms of metabolites,uric acid,as a metabolite,plays an important role in oxidative stress and neuroprotection in PD.It has been found that changes in uric acid levels may be associated with the onset and progression of PD,showing its potential as an early diagnostic marker.Epigenetic markers,such as DNA methylation modifications and miRNAs,have also attracted much attention in Parkinson’s disease research.Changes in these markers may affect the expression of PD-related genes and have an important impact on the onset and progression of the disease,providing new research perspectives for the early diagnosis of PD.In addition,exosomes,as a potential biomarker carrier for PD,are able to carry a variety of biomolecules involved in intercellular communication and pathological regulation.Studies have shown that exosomes may play an important role in the pathogenesis of PD,and their detection in blood may provide a new breakthrough for early diagnosis.It has been shown that exosomes may play an important role in the pathogenesis of PD,and their detection in blood may provide new breakthroughs in early diagnosis.In summary,through in-depth evaluation of biomarkers in the peripheral blood of PD patients,this paper demonstrates the important potential of these markers in the early diagnosis of PD and in the study of pathological mechanisms.Future studies will continue to explore the clinical application value of these biomarkers to promote the early detection of PD and individualized treatment strategies.展开更多
Objective:Neuropathic pain(NP)is one of the most common forms of chronic pain,yet current treatment options are limited in effectiveness.Peripheral nerve injury activates spinal microglia,altering their inflammatory r...Objective:Neuropathic pain(NP)is one of the most common forms of chronic pain,yet current treatment options are limited in effectiveness.Peripheral nerve injury activates spinal microglia,altering their inflammatory response and phagocytic functions,which contributes to the progression of NP.Most current research on NP focuses on microglial inflammation,with relatively little attention to their phagocytic function.Early growth response factor 2(EGR2)has been shown to regulate microglial phagocytosis,but its specific role in NP remains unclear.This study aims to investigate how EGR2 modulates microglial phagocytosis and its involvement in NP,with the goal of identifying potential therapeutic targets.Methods:Adult male Sprague-Dawley(SD)rats were used to establish a chronic constriction injury(CCI)model of the sciatic nerve.Pain behaviors were assessed on days 1,3,7,10,and 14 post-surgery to confirm successful model induction.The temporal and spatial expression of EGR2 in the spinal cord was examined using real-time quantitative PCR(RT-qPCR),Western blotting,and immunofluorescence staining.Adeno-associated virus(AAV)was used to overexpress EGR2 in the spinal cord,and behavioral assessments were performed to evaluate the effects of EGR2 modulation of NP.CCI and lipopolysaccharide(LPS)models were established in animals and microglial cell lines,respectively,and changes in phagocytic activity were measured using RT-qPCR and fluorescent latex bead uptake assays.After confirming the involvement of microglial phagocytosis in NP,AAV was used to overexpress EGR2 in both in vivo and in vitro models,and phagocytic activity was further evaluated.Finally,eukaryotic transcriptome sequencing was conducted to screen differentially expressed mRNAs,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses to identify potential downstream effectors of EGR2.Results:The CCI model successfully induced NP.Following CCI,EGR2 expression in the spinal cord was upregulated in parallel with NP development.Overexpression of EGR2 via spinal AAV injection enhanced microglial phagocytic activity and increased pain hypersensitivity in rats.Both animal and cellular models showed that CCI or LPS stimulation enhanced microglial phagocytosis,which was further amplified by EGR2 overexpression.Transcriptomic analysis of spinal cord tissues from CCI rats overexpressing EGR2 revealed upregulation of numerous genes associated with microglial phagocytosis and pain regulation.Among them,Lag3 emerged as a potential downstream target of EGR2.Conclusion:EGR2 contributes to the maintenance of NP by enhancing microglial phagocytosis in the spinal dorsal horn.展开更多
With the continuous expansion of deep underground engineering and the growing demand for safety monitoring,microseismic monitoring has become a core method for early warning of rock mass fracture and engineering stabi...With the continuous expansion of deep underground engineering and the growing demand for safety monitoring,microseismic monitoring has become a core method for early warning of rock mass fracture and engineering stability assessment.To address problems in existing methods,such as low data processing efficiency and poor phase recognition accuracy under low signal-to-noise ratio(SNR)conditions in complex geological environments,this study proposes an intelligent phase picking model based on ResUNet.The model integrates the residual learning mechanism of ResNet with the multi-scale feature extraction capability of UNet,effectively mitigating the vanishing gradient problem in deep networks.It also achieves cross-layer fusion of shallow detail features and deep semantic features through skip connections in the encoder-decoder structure.Compared with traditional short-time average/long-time average(STA/LTA)algorithms and advanced neural network models such as PhaseNet and EQTransformer,ResUNet shows superior performance in picking P-and S-wave phases.The model was trained on 400000 labeled microseismic signals from the Stanford earthquake dataset(STEAD)and was successfully applied to the Shizhuyuan polymetallic mine in Hunan Province,China.The results demonstrate that ResUNet achieves high picking accuracy and robustness in complex geological conditions,offering reliable technical support for early warning of disasters such as rockburst in deep underground engineering.展开更多
A single nucleotide polymorphism is the simplest form of genetic variation among individuals and can induce minor changes in phenotypic,physiological and biochemical characteristics.This polymorphism induces various m...A single nucleotide polymorphism is the simplest form of genetic variation among individuals and can induce minor changes in phenotypic,physiological and biochemical characteristics.This polymorphism induces various mutations that alter the sequence of a gene which can lead to observed changes in amino acids.Several assays have been developed for identification and validation of these markers.Each method has its own advantages and disadvantages but genotyping by sequencing is the most common and most widely used assay.These markers are also associated with several desirable traits like yield,fibre quality,boll size and genes respond to biotic and abiotic stresses in cotton.Changes in yield related traits are of interest to plant breeders.Numerous quantitative trait loci with novel functions have been identified in cotton by using these markers.This information can be used for crop improvement through molecular breeding approaches.In this review,we discuss the identification of these markers and their effects on gene function of economically important traits in cotton.展开更多
文摘Suid is one of the most common taxa among the Pleistocene mammalian fauna in China.However,its phylogenetic taxonomy and evolution are far from clear.The newly recovered suid fossil materials from the Early Pleistocene Shanshenmiaozui site in Nihewan Basin in North China provide new insight into the evolution of its kind in North China.The new materials include partial skulls and mandibles of males,and most of their teeth were preserved in situ.The specimens can be referred to the species Sus lydekkeri,which is the only species of Sus found in North China from the Early Pleistocene.The fossils of the once reported Pleistocene species“Potamochoerus chinhsienensis”should also be provisionally included in the species S.lydekkeri according to its form and size,which at least now excludes it from Potamochoerus.The species S.lydekkeri is very close to the extant species S.scrofa,and even was combined into the latter as a chronological subspecies by some authors,while its larger size,prominent preorbital fossa,backwardly positioned infraorbital foramen,frequently appearing verrucosic type(at least the intermediate type of the male lower canine),and simple crown structures support its validness as an independent species.The Early Pleistocene forms are characterized by a larger size,lower L/W ratio of cheekteeth,both upper and lower M3s having no more than three lobes,and the male lower canine is exclusively of a verrucosic type.Suid fossils are a very good indicator of paleoenvironments and paleoclimate.Both the northern and the southern faunas in China contain suid fossils throughout the Pleistocene Epoch,while the southern fauna has prominently more diversified taxa and more abundant fossil collections;whether that is caused by different origins or by environmental modifications is still not clear.
文摘The micromammal fossils collected from the Shangzhuang Formation of the Linxia Basin,Gansu,are described here.The assemblage consists of 16 species of 13 genera belonging to 7 families,including one new species and one taxon previously unknown from China.Among them 5 genera are Oligocene holdovers and 11 genera/species are known to make their first appearances in Early Miocene,indicating an Early Miocene age for the Gucheng Fauna.The absence of Oligocene survivors such as Tataromys,Yindirtemys,Eucricetodon and Tachyoryctoides,which usually occur in the Xiejian age of Early Miocene,and the presence of newcomers Protalactaga,Megacricetodon and Gobicricetodon,that made their first appearance only in the Shanwangian age of late Early Miocene,suggest that the Gucheng Fauna is of a later age of Early Miocene.The new species,Sinolagomys guchengensis,showing more derived morphology than in S.ulunguensis,seems to support an assessment of the fauna in Shanwangian age.Thus,the Gucheng Fauna may belong to late Early Miocene Shanwangian,partially equivalent to MN 3/4 of the European land mammal zonation.Ecological analysis of the fauna indicates that the palaeoecological condition of the Gucheng area might still be a predominantly arid shrub-steppe in late Early Miocene.
文摘Parkinson’s disease(PD)is a common neurodegenerative disorder with profound impact on patients’quality of life and long-term health,and early detection and intervention are particularly critical.In recent years,the search for precise and reliable biomarkers has become one of the key strategies to effectively address the clinical challenges of PD.In this paper,we systematically evaluated potential biomarkers,including proteins,metabolites,epigenetic markers,and exosomes,in the peripheral blood of PD patients.Protein markers are one of the main directions of biomarker research in PD.In particular,α‑synuclein and its phosphorylated form play a key role in the pathological process of PD.It has been shown that aggregation ofα-synuclein may be associated with pathologic protein deposition in PD and may be a potential marker for early diagnosis of PD.In terms of metabolites,uric acid,as a metabolite,plays an important role in oxidative stress and neuroprotection in PD.It has been found that changes in uric acid levels may be associated with the onset and progression of PD,showing its potential as an early diagnostic marker.Epigenetic markers,such as DNA methylation modifications and miRNAs,have also attracted much attention in Parkinson’s disease research.Changes in these markers may affect the expression of PD-related genes and have an important impact on the onset and progression of the disease,providing new research perspectives for the early diagnosis of PD.In addition,exosomes,as a potential biomarker carrier for PD,are able to carry a variety of biomolecules involved in intercellular communication and pathological regulation.Studies have shown that exosomes may play an important role in the pathogenesis of PD,and their detection in blood may provide a new breakthrough for early diagnosis.It has been shown that exosomes may play an important role in the pathogenesis of PD,and their detection in blood may provide new breakthroughs in early diagnosis.In summary,through in-depth evaluation of biomarkers in the peripheral blood of PD patients,this paper demonstrates the important potential of these markers in the early diagnosis of PD and in the study of pathological mechanisms.Future studies will continue to explore the clinical application value of these biomarkers to promote the early detection of PD and individualized treatment strategies.
基金supported by the National Natural Science Foundation of China(82071249 and 81771207).
文摘Objective:Neuropathic pain(NP)is one of the most common forms of chronic pain,yet current treatment options are limited in effectiveness.Peripheral nerve injury activates spinal microglia,altering their inflammatory response and phagocytic functions,which contributes to the progression of NP.Most current research on NP focuses on microglial inflammation,with relatively little attention to their phagocytic function.Early growth response factor 2(EGR2)has been shown to regulate microglial phagocytosis,but its specific role in NP remains unclear.This study aims to investigate how EGR2 modulates microglial phagocytosis and its involvement in NP,with the goal of identifying potential therapeutic targets.Methods:Adult male Sprague-Dawley(SD)rats were used to establish a chronic constriction injury(CCI)model of the sciatic nerve.Pain behaviors were assessed on days 1,3,7,10,and 14 post-surgery to confirm successful model induction.The temporal and spatial expression of EGR2 in the spinal cord was examined using real-time quantitative PCR(RT-qPCR),Western blotting,and immunofluorescence staining.Adeno-associated virus(AAV)was used to overexpress EGR2 in the spinal cord,and behavioral assessments were performed to evaluate the effects of EGR2 modulation of NP.CCI and lipopolysaccharide(LPS)models were established in animals and microglial cell lines,respectively,and changes in phagocytic activity were measured using RT-qPCR and fluorescent latex bead uptake assays.After confirming the involvement of microglial phagocytosis in NP,AAV was used to overexpress EGR2 in both in vivo and in vitro models,and phagocytic activity was further evaluated.Finally,eukaryotic transcriptome sequencing was conducted to screen differentially expressed mRNAs,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses to identify potential downstream effectors of EGR2.Results:The CCI model successfully induced NP.Following CCI,EGR2 expression in the spinal cord was upregulated in parallel with NP development.Overexpression of EGR2 via spinal AAV injection enhanced microglial phagocytic activity and increased pain hypersensitivity in rats.Both animal and cellular models showed that CCI or LPS stimulation enhanced microglial phagocytosis,which was further amplified by EGR2 overexpression.Transcriptomic analysis of spinal cord tissues from CCI rats overexpressing EGR2 revealed upregulation of numerous genes associated with microglial phagocytosis and pain regulation.Among them,Lag3 emerged as a potential downstream target of EGR2.Conclusion:EGR2 contributes to the maintenance of NP by enhancing microglial phagocytosis in the spinal dorsal horn.
基金Project(2022YFC2905100)supported by the National Key Research and Development Program of ChinaProject(52174098)supported by the National Natural Science Foundation of China。
文摘With the continuous expansion of deep underground engineering and the growing demand for safety monitoring,microseismic monitoring has become a core method for early warning of rock mass fracture and engineering stability assessment.To address problems in existing methods,such as low data processing efficiency and poor phase recognition accuracy under low signal-to-noise ratio(SNR)conditions in complex geological environments,this study proposes an intelligent phase picking model based on ResUNet.The model integrates the residual learning mechanism of ResNet with the multi-scale feature extraction capability of UNet,effectively mitigating the vanishing gradient problem in deep networks.It also achieves cross-layer fusion of shallow detail features and deep semantic features through skip connections in the encoder-decoder structure.Compared with traditional short-time average/long-time average(STA/LTA)algorithms and advanced neural network models such as PhaseNet and EQTransformer,ResUNet shows superior performance in picking P-and S-wave phases.The model was trained on 400000 labeled microseismic signals from the Stanford earthquake dataset(STEAD)and was successfully applied to the Shizhuyuan polymetallic mine in Hunan Province,China.The results demonstrate that ResUNet achieves high picking accuracy and robustness in complex geological conditions,offering reliable technical support for early warning of disasters such as rockburst in deep underground engineering.
文摘A single nucleotide polymorphism is the simplest form of genetic variation among individuals and can induce minor changes in phenotypic,physiological and biochemical characteristics.This polymorphism induces various mutations that alter the sequence of a gene which can lead to observed changes in amino acids.Several assays have been developed for identification and validation of these markers.Each method has its own advantages and disadvantages but genotyping by sequencing is the most common and most widely used assay.These markers are also associated with several desirable traits like yield,fibre quality,boll size and genes respond to biotic and abiotic stresses in cotton.Changes in yield related traits are of interest to plant breeders.Numerous quantitative trait loci with novel functions have been identified in cotton by using these markers.This information can be used for crop improvement through molecular breeding approaches.In this review,we discuss the identification of these markers and their effects on gene function of economically important traits in cotton.
