Signal filtering and differential acquisition are classic yet challenging issues in control engineering.The discrete-time optimal control(DTOC)based on classic tracking differentiator(TD)can effectively extract differ...Signal filtering and differential acquisition are classic yet challenging issues in control engineering.The discrete-time optimal control(DTOC)based on classic tracking differentiator(TD)can effectively extract differentiation signals and filter signals,while eliminating the chattering problem that arises during the discretization of the continuous solution.However,under external disturbance,the convergence mode may change,leading to overshoot and noise amplification.In this paper,a dual-switching strategy is proposed,which can alternate between the base double-integral system and its dual system according to the quadrant of the system’s state.And a novel linearized control law is also introduced,deriving a novel dual-switch tracking differentiator.Further analysis of system convergence and time optimality is provided.Simulation results show that the application of this dual-switching strategy notably reduces overshoot in both tracking and differential signals while enhancing noise filtering performance.Moreover,experiments conducted on a permanent magnet synchronous motor(PMSM)platform,where the proposed TD acts as a filter in the speed feedback loop,demonstrate that the standard deviation between the reference speed and the target speed(at a constant speed of 378 r/min)decreased from 5.63 r/min to 4.93 r/min,compared to the moving average algorithm.展开更多
When the maneuverability of a pursuer is not significantly higher than that of an evader,it will be difficult to intercept the evader with only one pursuer.Therefore,this article adopts a two-to-one differential game ...When the maneuverability of a pursuer is not significantly higher than that of an evader,it will be difficult to intercept the evader with only one pursuer.Therefore,this article adopts a two-to-one differential game strategy,the game of kind is generally considered to be angle-optimized,which allows unlimited turns,but these practices do not take into account the effect of acceleration,which does not correspond to the actual situation,thus,based on the angle-optimized,the acceleration optimization and the acceleration upper bound constraint are added into the game for consideration.A two-to-one differential game problem is proposed in the three-dimensional space,and an improved multi-objective grey wolf optimization(IMOGWO)algorithm is proposed to solve the optimal game point of this problem.With the equations that describe the relative motions between the pursuers and the evader in the three-dimensional space,a multi-objective function with constraints is given as the performance index to design an optimal strategy for the differential game.Then the optimal game point is solved by using the IMOGWO algorithm.It is proved based on Markov chains that with the IMOGWO,the Pareto solution set is the solution of the differential game.Finally,it is verified through simulations that the pursuers can capture the escapee,and via comparative experiments,it is shown that the IMOGWO algorithm performs well in terms of running time and memory usage.展开更多
Objective Cerebral palsy(CP)is a prevalent neurodevelopmental disorder acquired during the perinatal period,with periventricular white matter injury(PWMI)serving as its primary pathological hallmark.PWMI is characteri...Objective Cerebral palsy(CP)is a prevalent neurodevelopmental disorder acquired during the perinatal period,with periventricular white matter injury(PWMI)serving as its primary pathological hallmark.PWMI is characterized by the loss of oligodendrocytes(OLs)and the disintegration of myelin sheaths,leading to impaired neural connectivity and motor dysfunction.Neural stem cells(NSCs)represent a promising regenerative source for replenishing lost OLs;however,conventional twodimensional(2D)in vitro culture systems lack the three-dimensional(3D)physiological microenvironment.Microfluidic chip technology has emerged as a powerful tool to overcome this limitation by enabling precise spatial and temporal control over 3D microenvironmental conditions,including the establishment of stable concentration gradients of bioactive molecules.Catalpol,an iridoid glycoside derived from traditional medicinal plants,exhibits dual antioxidant and anti-apoptotic properties.Despite its therapeutic potential,the capacity of catalpol to drive NSC differentiation toward OLs under biomimetic 3D conditions,as well as the underlying molecular mechanisms,remains poorly understood.This study aims to develop a microfluidic-based 3D biomimetic platform to systematically investigate the concentration-dependent effects of catalpol on promoting NSCs-to-OLs differentiation and to elucidate the role of the caveolin-1(Cav-1)signaling pathway in this process.Methods We developed a novel multiplexed microfluidic device featuring parallel microchannels with integrated gradient generators capable of establishing and maintaining precise linear concentration gradients(0-3 g/L catalpol)across 3D NSCs cultures.This platform facilitated the continuous perfusion culture of NSC-derived 3D spheroids,mimicking the dynamic in vivo microenvironment.Real-time cell viability was assessed using Calcein-AM/propidium iodide(PI)dual staining,with fluorescence imaging quantifying live/dead cell ratios.Oligodendrocyte differentiation was evaluated through quantitative reverse transcription polymerase chain reaction(qRT-PCR)for MBP and SOX10 gene expression,complemented by immunofluorescence staining to visualize corresponding protein changes.To dissect the molecular mechanism,the Cav-1-specific pharmacological inhibitor methyl‑β‑cyclodextrin(MCD)was employed to perturb the pathway,and its effects on differentiation markers were analyzed.Results Catalpol demonstrated excellent biocompatibility,with cell viability exceeding 96%across the entire tested concentration range(0-3 g/L),confirming its non-cytotoxic nature.At the optimal concentration of 0-3 g/L,catalpol significantly upregulated both MBP and SOX10 expression(P<0.05,P<0.01),indicating robust promotion of oligodendroglial differentiation.Intriguingly,Cav-1 mRNA expression was progressively downregulated during NSC differentiation into OLs.Further inhibition of Cav-1 with MCD further enhanced this effect,leading to a statistically significant increase in OL-specific gene expression(P<0.05,P<0.01),suggesting Cav-1 acts as a negative regulator of OLs differentiation.Conclusion This study established an integrated microfluidic gradient chip-3D NSC spheroid culture system,which combines the advantages of precise chemical gradient control with physiologically relevant 3D cell culture.The findings demonstrate that 3 g/L catalpol effectively suppresses Cav-1 signaling to drive NSC differentiation into functional OLs.This work not only provides novel insights into the Cav-1-dependent mechanisms of myelination but also delivers a scalable technological platform for future research on remyelination therapies,with potential applications in cerebral palsy and other white matter disorders.The platform’s modular design permits adaptation for screening other neurogenic compounds or investigating additional signaling pathways involved in OLs maturation.展开更多
The boundness and compactness of products of multiplication,composition and differentiation on weighted Bergman spaces in the unit ball are studied.We define the differentiation operator on the space of holomorphic fu...The boundness and compactness of products of multiplication,composition and differentiation on weighted Bergman spaces in the unit ball are studied.We define the differentiation operator on the space of holomorphic functions in the unit ball by radial derivative.Then we extend the Sharma's results.展开更多
This paper studies the Smoluchowski–Kramers approximation for a discrete-time dynamical system modeled as the motion of a particle in a force field.We show that the approximation holds for the drift-implicit Euler–M...This paper studies the Smoluchowski–Kramers approximation for a discrete-time dynamical system modeled as the motion of a particle in a force field.We show that the approximation holds for the drift-implicit Euler–Maruyama discretization and derive its convergence rate.In particular,the solution of the discretized system converges to the solution of the first-order limit equation in the mean-square sense,and this convergence is independent of the order in which the mass parameterμand the step size h tend to zero.展开更多
Objective:To evaluate the predictive value of the neutrophil⁃to⁃lymphocyte ratio(NLR)and the systemic immune⁃inflammation index(SII)in predicting patients with anti⁃melanoma differentiation⁃associated gene 5⁃positive(...Objective:To evaluate the predictive value of the neutrophil⁃to⁃lymphocyte ratio(NLR)and the systemic immune⁃inflammation index(SII)in predicting patients with anti⁃melanoma differentiation⁃associated gene 5⁃positive(anti⁃MDA5+)dermatomyositis(DM)develop into the rapidly progressive interstitial lung disease(RPILD).Methods:We retrospectively analyzed the clinical and laboratory data of 124 anti⁃MDA5+DM patients from the First Affiliated Hospital of Nanjing Medical University between March 2019 and September 2023.We identified independent risk factors associated with the development and mortality of RPILD with the Cox regression analysis,and determined the optimal cut⁃off values for predicting adverse outcomes with the receiver operating characteristic(ROC)curve analysis.Results:Among the 124 patients,36 patients(29.03%)developed RPILD,and 39 patients(31.45%)died during the follow⁃up period.The results of multivariate Cox regression analysis showed that the elevated NLR was an independent risk factor for RPILD development,while the elevated SII expression was independently associated with the increased mortality of RPILD.Based on the ROC curve analysis,NLR>6.12 was a predictor for RPILD,and SII>875.79 was associated with increased mortality risk of RPILD.Conclusion:Both NLR and SII are accessible,cost⁃effective,and reliable prognostic indicators for the prognosis of patients with anti⁃MDA5^(+)DM,providing a valuable guidance for clinical management and risk stratification of the disease.展开更多
Dynamic soaring,inspired by the wind-riding flight of birds such as albatrosses,is a biomimetic technique which leverages wind fields to enhance the endurance of unmanned aerial vehicles(UAVs).Achieving a precise soar...Dynamic soaring,inspired by the wind-riding flight of birds such as albatrosses,is a biomimetic technique which leverages wind fields to enhance the endurance of unmanned aerial vehicles(UAVs).Achieving a precise soaring trajectory is crucial for maximizing energy efficiency during flight.Existing nonlinear programming methods are heavily dependent on the choice of initial values which is hard to determine.Therefore,this paper introduces a deep reinforcement learning method based on a differentially flat model for dynamic soaring trajectory planning and optimization.Initially,the gliding trajectory is parameterized using Fourier basis functions,achieving a flexible trajectory representation with a minimal number of hyperparameters.Subsequently,the trajectory optimization problem is formulated as a dynamic interactive process of Markov decision-making.The hyperparameters of the trajectory are optimized using the Proximal Policy Optimization(PPO2)algorithm from deep reinforcement learning(DRL),reducing the strong reliance on initial value settings in the optimization process.Finally,a comparison between the proposed method and the nonlinear programming method reveals that the trajectory generated by the proposed approach is smoother while meeting the same performance requirements.Specifically,the proposed method achieves a 34%reduction in maximum thrust,a 39.4%decrease in maximum thrust difference,and a 33%reduction in maximum airspeed difference.展开更多
This paper presents a mode-switching collaborative defense strategy for spacecraft pursuit-evasiondefense scenarios.In these scenarios,the pursuer tries to avoid the defender while capturing the evader,while the evade...This paper presents a mode-switching collaborative defense strategy for spacecraft pursuit-evasiondefense scenarios.In these scenarios,the pursuer tries to avoid the defender while capturing the evader,while the evader and defender form an alliance to prevent the pursuer from achieving its goal.First,the behavioral modes of the pursuer,including attack and avoidance modes,were established using differential game theory.These modes are then recognized by an interactive multiple model-matching algorithm(IMM),that uses several smooth variable structure filters to match the modes of the pursuer and update their probabilities in real time.Based on the linear-quadratic optimization theory,combined with the results of strategy identification,a two-way cooperative optimal strategy for the defender and evader is proposed,where the evader aids the defender to intercept the pursuer by performing luring maneuvers.Simulation results show that the interactive multi-model algorithm based on several smooth variable structure filters perform well in the strategy identification of the pursuer,and the cooperative defense strategy based on strategy identification has good interception performance when facing pursuers,who are able to flexibly adjust their game objectives.展开更多
This paper proposes a distributed event-triggered control(ETC)framework to address cooperative target fencing challenges in UAV swarm.The proposed architecture eliminates the reliance on preset formation parameters wh...This paper proposes a distributed event-triggered control(ETC)framework to address cooperative target fencing challenges in UAV swarm.The proposed architecture eliminates the reliance on preset formation parameters while achieving multi-objective cooperative control for target fencing,network connectivity preservation,collision avoidance,and communication efficiency optimization.Firstly,a differential state observer is constructed to obtain the target's unmeasurable states.Secondly,leveraging swarm selforganization principles,a geometric-constraint-free distributed fencing controller is designed by integrating potential field methods with consensus theory.The controller dynamically adjusts inter-UAV distances via single potential function,enabling coordinated optimization of persistent network connectivity and collision-free motion during target fencing.Thirdly,a dual-threshold ETC mechanism based on velocity consensus deviation and fencing error is proposed,which can be triggered based on task features to dynamically adjust the communication frequency,significantly reduce the communication burden and exclude Zeno behavior.Theoretical analysis demonstrates the stability of closed-loop systems.Multi-scenario simulations show that the proposed method can achieve robust fencing under target maneuverability,partial UAV failures,and communication disturbances.展开更多
Objective To explore the value of contrast-enhanced ultrasound(CEUS)for predicting differentiation degree of hepatocellular carcinoma(HCC).Methods Totally 86 HCC patients confirmed by postoperative pathology were retr...Objective To explore the value of contrast-enhanced ultrasound(CEUS)for predicting differentiation degree of hepatocellular carcinoma(HCC).Methods Totally 86 HCC patients confirmed by postoperative pathology were retrospectively enrolled and divided into poorly differentiated,moderately differentiated and highly differentiated groups according to postoperative Edmondson-Steiner grading.Preoperative CEUS parameters were compared among groups,and binary logistic regression was used to analyze CEUS-related independent predictors of HCC with different differentiation.The receiver operating characteristic curves of parameters being significant different among groups were drawn,the areas under the curve(AUC)were calculated,and the efficacy for predicting HCC with different differentiation degree was evaluated.Results There were 29 cases in poorly differentiated group,37 in moderately differentiated group and 20 cases in highly differentiated group.The arrival time of contrast agent in poorly differentiated group was earlier than that in moderately and high differentiated groups(both P<0.05),while in moderately differentiated group was not significantly different with that in highly differentiated group(P>0.05).The washout grade were significantly different between each 2 groups(all P<0.05).The arrival time of contrast agent and washout grade were independent predictors of highly or poorly differentiated,moderately or poorly differentiated,moderate-highly or poorly differentiated HCC,and washout grade also was independent predictor of highly or moderately differentiated HCC(all P<0.05).The AUC of the arrival time of contrast agent for predicting highly or moderately differentiated,highly or poorly differentiated,moderately or poorly differentiated,moderate-highly or poorly differentiated HCC was 0.615,0.787,0.690 and 0.724,respectively,while of washout grade was 0.801,0.927,0.795 and 0.841,respectively.Conclusion CEUS could be used to effectively predict differentiation degree of HCC.展开更多
An innovative complex lidar system deployed on an airborne rotorcraft platform for remote sensing of atmospheric pollution is proposed and demonstrated.The system incorporates integrated-path differential absorption l...An innovative complex lidar system deployed on an airborne rotorcraft platform for remote sensing of atmospheric pollution is proposed and demonstrated.The system incorporates integrated-path differential absorption lidar(DIAL) and coherent-doppler lidar(CDL) techniques using a dual tunable TEA CO_(2)laser in the 9—11 μm band and a 1.55 μm fiber laser.By combining the principles of differential absorption detection and pulsed coherent detection,the system enables agile and remote sensing of atmospheric pollution.Extensive static tests validate the system’s real-time detection capabilities,including the measurement of concentration-path-length product(CL),front distance,and path wind speed of air pollution plumes over long distances exceeding 4 km.Flight experiments is conducted with the helicopter.Scanning of the pollutant concentration and the wind field is carried out in an approximately 1 km slant range over scanning angle ranges from 45°to 65°,with a radial resolution of 30 m and10 s.The test results demonstrate the system’s ability to spatially map atmospheric pollution plumes and predict their motion and dispersion patterns,thereby ensuring the protection of public safety.展开更多
Missile interception problem can be regarded as a two-person zero-sum differential games problem,which depends on the solution of Hamilton-Jacobi-Isaacs(HJI)equa-tion.It has been proved impossible to obtain a closed-f...Missile interception problem can be regarded as a two-person zero-sum differential games problem,which depends on the solution of Hamilton-Jacobi-Isaacs(HJI)equa-tion.It has been proved impossible to obtain a closed-form solu-tion due to the nonlinearity of HJI equation,and many iterative algorithms are proposed to solve the HJI equation.Simultane-ous policy updating algorithm(SPUA)is an effective algorithm for solving HJI equation,but it is an on-policy integral reinforce-ment learning(IRL).For online implementation of SPUA,the dis-turbance signals need to be adjustable,which is unrealistic.In this paper,an off-policy IRL algorithm based on SPUA is pro-posed without making use of any knowledge of the systems dynamics.Then,a neural-network based online adaptive critic implementation scheme of the off-policy IRL algorithm is pre-sented.Based on the online off-policy IRL method,a computa-tional intelligence interception guidance(CIIG)law is developed for intercepting high-maneuvering target.As a model-free method,intercepting targets can be achieved through measur-ing system data online.The effectiveness of the CIIG is verified through two missile and target engagement scenarios.展开更多
Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature ...Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature only demonstrated the optimality of group testing strategy while estimating prevalence under some strong assumptions.This article weakens the assumption of misclassification rate in the previous literature,considers the misclassification rate of the infected samples as a differentiable function of the pool size,and explores some optimal properties of group testing for estimating prevalence in the presence of differential misclassification conforming to this assumption.This article theoretically demonstrates that the group testing strategy performs better than the sample by sample procedure in estimating disease prevalence when the total number of sample pools is given or the size of the test population is determined.Numerical simulation experiments were conducted to evaluate the performance of group tests in estimating prevalence in the presence of dilution effect.展开更多
To solve the problem of increased computation and communication costs caused by using homomorphic encryption(HE) to protect all gradients in traditional cryptographic aggregation(cryptoaggregation) schemes,a fast cryp...To solve the problem of increased computation and communication costs caused by using homomorphic encryption(HE) to protect all gradients in traditional cryptographic aggregation(cryptoaggregation) schemes,a fast crypto-aggregation scheme called RandomCrypt was proposed.RandomCrypt performed clipping and quantization to fix the range of gradient values and then added two types of noise on the gradient for encryption and differential privacy(DP) protection.It conducted HE on noise keys to revise the precision loss caused by DP protection.RandomCrypt was implemented based on a FATE framework,and a hacking simulation experiment was conducted.The results show that the proposed scheme can effectively hinder inference attacks while ensuring training accuracy.It only requires 45%~51% communication cost and 5%~23% computation cost compared with traditional schemes.展开更多
A differential game guidance scheme with obstacle avoidance,based on the formulation of a combined linear quadratic and norm-bounded differential game,is designed for a three-player engagement scenario,which includes ...A differential game guidance scheme with obstacle avoidance,based on the formulation of a combined linear quadratic and norm-bounded differential game,is designed for a three-player engagement scenario,which includes a pursuer,an interceptor,and an evader.The confrontation between the players is divided into four phases(P1-P4)by introducing the switching time,and proposing different guidance strategies according to the phase where the static obstacle is located:the linear quadratic game method is employed to devise the guidance scheme for the energy optimization when the obstacle is located in the P1 and P3 stages;the norm-bounded differential game guidance strategy is presented to satisfy the acceleration constraint under the circumstance that the obstacle is located in the P2 and P4 phases.Furthermore,the radii of the static obstacle and the interceptor are taken as the design parameters to derive the combined guidance strategy through the dead-zone function,which guarantees that the pursuer avoids the static obstacle,and the interceptor,and attacks the evader.Finally,the nonlinear numerical simulations verify the performance of the game guidance strategy.展开更多
Angles-only relative orbit determination for space non-cooperative targets based on passive sensor is subject to weakly observable problem of the relative state between two spacecraft. Previously, the evidence for ang...Angles-only relative orbit determination for space non-cooperative targets based on passive sensor is subject to weakly observable problem of the relative state between two spacecraft. Previously, the evidence for angles-only observability was found by using cylindrical dynamics, however, the solution of orbit determination is still not provided. This study develops a relative orbit determination algorithm with the cylindrical dynamics based on differential evolution. Firstly, the relative motion dynamics and line-of-sight measurement model for nearcircular orbit are established in cylindrical coordinate system.Secondly, the observability is qualitatively analyzed by using the dynamics and measurement model where the unobservable geometry is found. Then, the angles-only relative orbit determination problem is modeled into an optimal searching frame and an improved differential evolution algorithm is introduced to solve the problem. Finally, the proposed algorithm is verified and tested by a set of numerical simulations in the context of highEarth and low-Earth cases. The results show that initial relative orbit determination(IROD) solution with an appropriate accuracy in a relative short span is achieved, which can be used to initialize the navigation filter.展开更多
In the realm of aerial warfare,the protection of Unmanned Aerial Vehicles(UAVs) against adversarial threats is crucial.In order to balance the impact of response delays and the demand for onboard applications,this pap...In the realm of aerial warfare,the protection of Unmanned Aerial Vehicles(UAVs) against adversarial threats is crucial.In order to balance the impact of response delays and the demand for onboard applications,this paper derives three analytical game strategies for the active defense of UAVs from differential game theory,accommodating the first-order dynamic delays.The targeted UAV executes evasive maneuvers and launches a defending missile to intercept the attacking missile,which constitutes a UAVMissile-Defender(UMD) three-body game problem.We explore two distinct operational paradigms:the first involves the UAV and the defender working collaboratively to intercept the incoming threat,while the second prioritizes UAV self-preservation,with independent maneuvering away from potentially sacrificial engagements.Starting with model linearization and order reduction,the Collaborative Interception Strategy(CIS) is first derived via a linear quadratic differential game formulation.Building upon CIS,we further explore two distinct strategies:the Informed Defender Interception Strategy(IDIS),which utilizes UAV maneuvering information,and the Unassisted Defender Interception Strategy(UDIS),which does not rely on UAV maneuvering information.Additionally,we investigate the conditions for the existence of saddle point solutions and their relationship with vehicle maneuverability and response agility.The simulations demonstrate the effectiveness and advantages of the proposed strategies.展开更多
基金Project(QZKFKT2023-012)supported by the State Key Laboratory of Heavy-duty and Express High-power Electric Locomotive,China。
文摘Signal filtering and differential acquisition are classic yet challenging issues in control engineering.The discrete-time optimal control(DTOC)based on classic tracking differentiator(TD)can effectively extract differentiation signals and filter signals,while eliminating the chattering problem that arises during the discretization of the continuous solution.However,under external disturbance,the convergence mode may change,leading to overshoot and noise amplification.In this paper,a dual-switching strategy is proposed,which can alternate between the base double-integral system and its dual system according to the quadrant of the system’s state.And a novel linearized control law is also introduced,deriving a novel dual-switch tracking differentiator.Further analysis of system convergence and time optimality is provided.Simulation results show that the application of this dual-switching strategy notably reduces overshoot in both tracking and differential signals while enhancing noise filtering performance.Moreover,experiments conducted on a permanent magnet synchronous motor(PMSM)platform,where the proposed TD acts as a filter in the speed feedback loop,demonstrate that the standard deviation between the reference speed and the target speed(at a constant speed of 378 r/min)decreased from 5.63 r/min to 4.93 r/min,compared to the moving average algorithm.
基金National Natural Science Foundation of China(NSFC61773142,NSFC62303136)。
文摘When the maneuverability of a pursuer is not significantly higher than that of an evader,it will be difficult to intercept the evader with only one pursuer.Therefore,this article adopts a two-to-one differential game strategy,the game of kind is generally considered to be angle-optimized,which allows unlimited turns,but these practices do not take into account the effect of acceleration,which does not correspond to the actual situation,thus,based on the angle-optimized,the acceleration optimization and the acceleration upper bound constraint are added into the game for consideration.A two-to-one differential game problem is proposed in the three-dimensional space,and an improved multi-objective grey wolf optimization(IMOGWO)algorithm is proposed to solve the optimal game point of this problem.With the equations that describe the relative motions between the pursuers and the evader in the three-dimensional space,a multi-objective function with constraints is given as the performance index to design an optimal strategy for the differential game.Then the optimal game point is solved by using the IMOGWO algorithm.It is proved based on Markov chains that with the IMOGWO,the Pareto solution set is the solution of the differential game.Finally,it is verified through simulations that the pursuers can capture the escapee,and via comparative experiments,it is shown that the IMOGWO algorithm performs well in terms of running time and memory usage.
基金supported by grants from the Liaoning Province Excellent Talent Program Project(XLYC1902031)Dalian Science and Technology Talent Innovation Plan Grant(2022RG18)Basic Research Project of the Department of Education of Liaoning Province(LJKQZ20222395)。
文摘Objective Cerebral palsy(CP)is a prevalent neurodevelopmental disorder acquired during the perinatal period,with periventricular white matter injury(PWMI)serving as its primary pathological hallmark.PWMI is characterized by the loss of oligodendrocytes(OLs)and the disintegration of myelin sheaths,leading to impaired neural connectivity and motor dysfunction.Neural stem cells(NSCs)represent a promising regenerative source for replenishing lost OLs;however,conventional twodimensional(2D)in vitro culture systems lack the three-dimensional(3D)physiological microenvironment.Microfluidic chip technology has emerged as a powerful tool to overcome this limitation by enabling precise spatial and temporal control over 3D microenvironmental conditions,including the establishment of stable concentration gradients of bioactive molecules.Catalpol,an iridoid glycoside derived from traditional medicinal plants,exhibits dual antioxidant and anti-apoptotic properties.Despite its therapeutic potential,the capacity of catalpol to drive NSC differentiation toward OLs under biomimetic 3D conditions,as well as the underlying molecular mechanisms,remains poorly understood.This study aims to develop a microfluidic-based 3D biomimetic platform to systematically investigate the concentration-dependent effects of catalpol on promoting NSCs-to-OLs differentiation and to elucidate the role of the caveolin-1(Cav-1)signaling pathway in this process.Methods We developed a novel multiplexed microfluidic device featuring parallel microchannels with integrated gradient generators capable of establishing and maintaining precise linear concentration gradients(0-3 g/L catalpol)across 3D NSCs cultures.This platform facilitated the continuous perfusion culture of NSC-derived 3D spheroids,mimicking the dynamic in vivo microenvironment.Real-time cell viability was assessed using Calcein-AM/propidium iodide(PI)dual staining,with fluorescence imaging quantifying live/dead cell ratios.Oligodendrocyte differentiation was evaluated through quantitative reverse transcription polymerase chain reaction(qRT-PCR)for MBP and SOX10 gene expression,complemented by immunofluorescence staining to visualize corresponding protein changes.To dissect the molecular mechanism,the Cav-1-specific pharmacological inhibitor methyl‑β‑cyclodextrin(MCD)was employed to perturb the pathway,and its effects on differentiation markers were analyzed.Results Catalpol demonstrated excellent biocompatibility,with cell viability exceeding 96%across the entire tested concentration range(0-3 g/L),confirming its non-cytotoxic nature.At the optimal concentration of 0-3 g/L,catalpol significantly upregulated both MBP and SOX10 expression(P<0.05,P<0.01),indicating robust promotion of oligodendroglial differentiation.Intriguingly,Cav-1 mRNA expression was progressively downregulated during NSC differentiation into OLs.Further inhibition of Cav-1 with MCD further enhanced this effect,leading to a statistically significant increase in OL-specific gene expression(P<0.05,P<0.01),suggesting Cav-1 acts as a negative regulator of OLs differentiation.Conclusion This study established an integrated microfluidic gradient chip-3D NSC spheroid culture system,which combines the advantages of precise chemical gradient control with physiologically relevant 3D cell culture.The findings demonstrate that 3 g/L catalpol effectively suppresses Cav-1 signaling to drive NSC differentiation into functional OLs.This work not only provides novel insights into the Cav-1-dependent mechanisms of myelination but also delivers a scalable technological platform for future research on remyelination therapies,with potential applications in cerebral palsy and other white matter disorders.The platform’s modular design permits adaptation for screening other neurogenic compounds or investigating additional signaling pathways involved in OLs maturation.
基金Supported by Natural Science Foundation of Guangdong Province in China(2018KTSCX161)。
文摘The boundness and compactness of products of multiplication,composition and differentiation on weighted Bergman spaces in the unit ball are studied.We define the differentiation operator on the space of holomorphic functions in the unit ball by radial derivative.Then we extend the Sharma's results.
基金supported by the PhD Research Startup Foundation of Hubei University of Economics(Grand No.XJ23BS42).
文摘This paper studies the Smoluchowski–Kramers approximation for a discrete-time dynamical system modeled as the motion of a particle in a force field.We show that the approximation holds for the drift-implicit Euler–Maruyama discretization and derive its convergence rate.In particular,the solution of the discretized system converges to the solution of the first-order limit equation in the mean-square sense,and this convergence is independent of the order in which the mass parameterμand the step size h tend to zero.
文摘Objective:To evaluate the predictive value of the neutrophil⁃to⁃lymphocyte ratio(NLR)and the systemic immune⁃inflammation index(SII)in predicting patients with anti⁃melanoma differentiation⁃associated gene 5⁃positive(anti⁃MDA5+)dermatomyositis(DM)develop into the rapidly progressive interstitial lung disease(RPILD).Methods:We retrospectively analyzed the clinical and laboratory data of 124 anti⁃MDA5+DM patients from the First Affiliated Hospital of Nanjing Medical University between March 2019 and September 2023.We identified independent risk factors associated with the development and mortality of RPILD with the Cox regression analysis,and determined the optimal cut⁃off values for predicting adverse outcomes with the receiver operating characteristic(ROC)curve analysis.Results:Among the 124 patients,36 patients(29.03%)developed RPILD,and 39 patients(31.45%)died during the follow⁃up period.The results of multivariate Cox regression analysis showed that the elevated NLR was an independent risk factor for RPILD development,while the elevated SII expression was independently associated with the increased mortality of RPILD.Based on the ROC curve analysis,NLR>6.12 was a predictor for RPILD,and SII>875.79 was associated with increased mortality risk of RPILD.Conclusion:Both NLR and SII are accessible,cost⁃effective,and reliable prognostic indicators for the prognosis of patients with anti⁃MDA5^(+)DM,providing a valuable guidance for clinical management and risk stratification of the disease.
基金support received by the National Natural Science Foundation of China(Grant Nos.52372398&62003272).
文摘Dynamic soaring,inspired by the wind-riding flight of birds such as albatrosses,is a biomimetic technique which leverages wind fields to enhance the endurance of unmanned aerial vehicles(UAVs).Achieving a precise soaring trajectory is crucial for maximizing energy efficiency during flight.Existing nonlinear programming methods are heavily dependent on the choice of initial values which is hard to determine.Therefore,this paper introduces a deep reinforcement learning method based on a differentially flat model for dynamic soaring trajectory planning and optimization.Initially,the gliding trajectory is parameterized using Fourier basis functions,achieving a flexible trajectory representation with a minimal number of hyperparameters.Subsequently,the trajectory optimization problem is formulated as a dynamic interactive process of Markov decision-making.The hyperparameters of the trajectory are optimized using the Proximal Policy Optimization(PPO2)algorithm from deep reinforcement learning(DRL),reducing the strong reliance on initial value settings in the optimization process.Finally,a comparison between the proposed method and the nonlinear programming method reveals that the trajectory generated by the proposed approach is smoother while meeting the same performance requirements.Specifically,the proposed method achieves a 34%reduction in maximum thrust,a 39.4%decrease in maximum thrust difference,and a 33%reduction in maximum airspeed difference.
基金the Science and Technology Department,Heilongjiang Province under Grant Agreement No JJ2022LH0315。
文摘This paper presents a mode-switching collaborative defense strategy for spacecraft pursuit-evasiondefense scenarios.In these scenarios,the pursuer tries to avoid the defender while capturing the evader,while the evader and defender form an alliance to prevent the pursuer from achieving its goal.First,the behavioral modes of the pursuer,including attack and avoidance modes,were established using differential game theory.These modes are then recognized by an interactive multiple model-matching algorithm(IMM),that uses several smooth variable structure filters to match the modes of the pursuer and update their probabilities in real time.Based on the linear-quadratic optimization theory,combined with the results of strategy identification,a two-way cooperative optimal strategy for the defender and evader is proposed,where the evader aids the defender to intercept the pursuer by performing luring maneuvers.Simulation results show that the interactive multi-model algorithm based on several smooth variable structure filters perform well in the strategy identification of the pursuer,and the cooperative defense strategy based on strategy identification has good interception performance when facing pursuers,who are able to flexibly adjust their game objectives.
文摘This paper proposes a distributed event-triggered control(ETC)framework to address cooperative target fencing challenges in UAV swarm.The proposed architecture eliminates the reliance on preset formation parameters while achieving multi-objective cooperative control for target fencing,network connectivity preservation,collision avoidance,and communication efficiency optimization.Firstly,a differential state observer is constructed to obtain the target's unmeasurable states.Secondly,leveraging swarm selforganization principles,a geometric-constraint-free distributed fencing controller is designed by integrating potential field methods with consensus theory.The controller dynamically adjusts inter-UAV distances via single potential function,enabling coordinated optimization of persistent network connectivity and collision-free motion during target fencing.Thirdly,a dual-threshold ETC mechanism based on velocity consensus deviation and fencing error is proposed,which can be triggered based on task features to dynamically adjust the communication frequency,significantly reduce the communication burden and exclude Zeno behavior.Theoretical analysis demonstrates the stability of closed-loop systems.Multi-scenario simulations show that the proposed method can achieve robust fencing under target maneuverability,partial UAV failures,and communication disturbances.
文摘Objective To explore the value of contrast-enhanced ultrasound(CEUS)for predicting differentiation degree of hepatocellular carcinoma(HCC).Methods Totally 86 HCC patients confirmed by postoperative pathology were retrospectively enrolled and divided into poorly differentiated,moderately differentiated and highly differentiated groups according to postoperative Edmondson-Steiner grading.Preoperative CEUS parameters were compared among groups,and binary logistic regression was used to analyze CEUS-related independent predictors of HCC with different differentiation.The receiver operating characteristic curves of parameters being significant different among groups were drawn,the areas under the curve(AUC)were calculated,and the efficacy for predicting HCC with different differentiation degree was evaluated.Results There were 29 cases in poorly differentiated group,37 in moderately differentiated group and 20 cases in highly differentiated group.The arrival time of contrast agent in poorly differentiated group was earlier than that in moderately and high differentiated groups(both P<0.05),while in moderately differentiated group was not significantly different with that in highly differentiated group(P>0.05).The washout grade were significantly different between each 2 groups(all P<0.05).The arrival time of contrast agent and washout grade were independent predictors of highly or poorly differentiated,moderately or poorly differentiated,moderate-highly or poorly differentiated HCC,and washout grade also was independent predictor of highly or moderately differentiated HCC(all P<0.05).The AUC of the arrival time of contrast agent for predicting highly or moderately differentiated,highly or poorly differentiated,moderately or poorly differentiated,moderate-highly or poorly differentiated HCC was 0.615,0.787,0.690 and 0.724,respectively,while of washout grade was 0.801,0.927,0.795 and 0.841,respectively.Conclusion CEUS could be used to effectively predict differentiation degree of HCC.
文摘An innovative complex lidar system deployed on an airborne rotorcraft platform for remote sensing of atmospheric pollution is proposed and demonstrated.The system incorporates integrated-path differential absorption lidar(DIAL) and coherent-doppler lidar(CDL) techniques using a dual tunable TEA CO_(2)laser in the 9—11 μm band and a 1.55 μm fiber laser.By combining the principles of differential absorption detection and pulsed coherent detection,the system enables agile and remote sensing of atmospheric pollution.Extensive static tests validate the system’s real-time detection capabilities,including the measurement of concentration-path-length product(CL),front distance,and path wind speed of air pollution plumes over long distances exceeding 4 km.Flight experiments is conducted with the helicopter.Scanning of the pollutant concentration and the wind field is carried out in an approximately 1 km slant range over scanning angle ranges from 45°to 65°,with a radial resolution of 30 m and10 s.The test results demonstrate the system’s ability to spatially map atmospheric pollution plumes and predict their motion and dispersion patterns,thereby ensuring the protection of public safety.
文摘Missile interception problem can be regarded as a two-person zero-sum differential games problem,which depends on the solution of Hamilton-Jacobi-Isaacs(HJI)equa-tion.It has been proved impossible to obtain a closed-form solu-tion due to the nonlinearity of HJI equation,and many iterative algorithms are proposed to solve the HJI equation.Simultane-ous policy updating algorithm(SPUA)is an effective algorithm for solving HJI equation,but it is an on-policy integral reinforce-ment learning(IRL).For online implementation of SPUA,the dis-turbance signals need to be adjustable,which is unrealistic.In this paper,an off-policy IRL algorithm based on SPUA is pro-posed without making use of any knowledge of the systems dynamics.Then,a neural-network based online adaptive critic implementation scheme of the off-policy IRL algorithm is pre-sented.Based on the online off-policy IRL method,a computa-tional intelligence interception guidance(CIIG)law is developed for intercepting high-maneuvering target.As a model-free method,intercepting targets can be achieved through measur-ing system data online.The effectiveness of the CIIG is verified through two missile and target engagement scenarios.
基金supported by the National Natural Science Foundation of China(Grant No.72091212).
文摘Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature only demonstrated the optimality of group testing strategy while estimating prevalence under some strong assumptions.This article weakens the assumption of misclassification rate in the previous literature,considers the misclassification rate of the infected samples as a differentiable function of the pool size,and explores some optimal properties of group testing for estimating prevalence in the presence of differential misclassification conforming to this assumption.This article theoretically demonstrates that the group testing strategy performs better than the sample by sample procedure in estimating disease prevalence when the total number of sample pools is given or the size of the test population is determined.Numerical simulation experiments were conducted to evaluate the performance of group tests in estimating prevalence in the presence of dilution effect.
基金Beijing Natural Science Foundation (L233005)National Key Research and Development Program of China (2023YFB3308200)。
文摘To solve the problem of increased computation and communication costs caused by using homomorphic encryption(HE) to protect all gradients in traditional cryptographic aggregation(cryptoaggregation) schemes,a fast crypto-aggregation scheme called RandomCrypt was proposed.RandomCrypt performed clipping and quantization to fix the range of gradient values and then added two types of noise on the gradient for encryption and differential privacy(DP) protection.It conducted HE on noise keys to revise the precision loss caused by DP protection.RandomCrypt was implemented based on a FATE framework,and a hacking simulation experiment was conducted.The results show that the proposed scheme can effectively hinder inference attacks while ensuring training accuracy.It only requires 45%~51% communication cost and 5%~23% computation cost compared with traditional schemes.
基金supported by National Natural Science Foundation(NNSF)of China under(Grant No.62273119)。
文摘A differential game guidance scheme with obstacle avoidance,based on the formulation of a combined linear quadratic and norm-bounded differential game,is designed for a three-player engagement scenario,which includes a pursuer,an interceptor,and an evader.The confrontation between the players is divided into four phases(P1-P4)by introducing the switching time,and proposing different guidance strategies according to the phase where the static obstacle is located:the linear quadratic game method is employed to devise the guidance scheme for the energy optimization when the obstacle is located in the P1 and P3 stages;the norm-bounded differential game guidance strategy is presented to satisfy the acceleration constraint under the circumstance that the obstacle is located in the P2 and P4 phases.Furthermore,the radii of the static obstacle and the interceptor are taken as the design parameters to derive the combined guidance strategy through the dead-zone function,which guarantees that the pursuer avoids the static obstacle,and the interceptor,and attacks the evader.Finally,the nonlinear numerical simulations verify the performance of the game guidance strategy.
基金supported by the National Natural Science Foundation of China (12272168)the Foundation of Science and Technology on Space Intelligent Control Laboratory (HTKJ2023KL502015)。
文摘Angles-only relative orbit determination for space non-cooperative targets based on passive sensor is subject to weakly observable problem of the relative state between two spacecraft. Previously, the evidence for angles-only observability was found by using cylindrical dynamics, however, the solution of orbit determination is still not provided. This study develops a relative orbit determination algorithm with the cylindrical dynamics based on differential evolution. Firstly, the relative motion dynamics and line-of-sight measurement model for nearcircular orbit are established in cylindrical coordinate system.Secondly, the observability is qualitatively analyzed by using the dynamics and measurement model where the unobservable geometry is found. Then, the angles-only relative orbit determination problem is modeled into an optimal searching frame and an improved differential evolution algorithm is introduced to solve the problem. Finally, the proposed algorithm is verified and tested by a set of numerical simulations in the context of highEarth and low-Earth cases. The results show that initial relative orbit determination(IROD) solution with an appropriate accuracy in a relative short span is achieved, which can be used to initialize the navigation filter.
基金supported by the China Postdoctoral Science Foundation (Grant No.2021M700321)the Fundamental Research Funds for the Central Universities (Grant No.YWF-23-Q1041)。
文摘In the realm of aerial warfare,the protection of Unmanned Aerial Vehicles(UAVs) against adversarial threats is crucial.In order to balance the impact of response delays and the demand for onboard applications,this paper derives three analytical game strategies for the active defense of UAVs from differential game theory,accommodating the first-order dynamic delays.The targeted UAV executes evasive maneuvers and launches a defending missile to intercept the attacking missile,which constitutes a UAVMissile-Defender(UMD) three-body game problem.We explore two distinct operational paradigms:the first involves the UAV and the defender working collaboratively to intercept the incoming threat,while the second prioritizes UAV self-preservation,with independent maneuvering away from potentially sacrificial engagements.Starting with model linearization and order reduction,the Collaborative Interception Strategy(CIS) is first derived via a linear quadratic differential game formulation.Building upon CIS,we further explore two distinct strategies:the Informed Defender Interception Strategy(IDIS),which utilizes UAV maneuvering information,and the Unassisted Defender Interception Strategy(UDIS),which does not rely on UAV maneuvering information.Additionally,we investigate the conditions for the existence of saddle point solutions and their relationship with vehicle maneuverability and response agility.The simulations demonstrate the effectiveness and advantages of the proposed strategies.
