Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as bioch...Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as biochemical sensors for medicine,food safety and environmental monitoring.However,there is little research on aptamer-target binding mechanisms,which limits their application and development.Computational simulation has gained much attention for revealing aptamer-target binding mechanisms at the atomic level.This work summarizes the main simulation methods used in the mechanistic analysis of aptamer-target complexes,the characteristics of binding between aptamers and different targets(metal ions,small organic molecules,biomacromolecules,cells,bacteria and viruses),the types of aptamer-target interactions and the factors influencing their strength.It provides a reference for further use of simulations in understanding aptamer-target binding mechanisms.展开更多
Potassium ion(K^(+))is widely involved in several physiopathological processes,and its abnormal changes are closely related to the occurrence of brain diseases of cerebral ischemia.In vivo acquirement of K^(+)variatio...Potassium ion(K^(+))is widely involved in several physiopathological processes,and its abnormal changes are closely related to the occurrence of brain diseases of cerebral ischemia.In vivo acquirement of K^(+)variation is significant to understand the roles of K^(+)playing in brain functions.A microelectrode based on single-stranded DNA aptamers was developed for highly selective detection of K^(+)in brain,in which the aptamer probes were designed to contain an aptamer part for specific recognition of K^(+),an alkynyl group used for stable confinement of aptamer probe on the gold surface,and an electrochemical redox active ferrocene group to generate current response signal.The response range of the microelectrodes could be rationally tuned by varying the chain length of the aptamer probe.The optimized electrode,LAC,displayed high selectivity for in vivo detection of K^(+),and suitable linear range from 10 mmol·L-1e10 mmol·L-1,which could fulfill the requirement of K^(+)detection in brain.Eventually,the microelectrodes were successfully applied for the detection of K^(+)in the living mouse brains followed by hypoxic.展开更多
文摘Aptamers are a type of single-chain oligonucleotide that can combine with a specific target.Due to their simple preparation,easy modification,stable structure and reusability,aptamers have been widely applied as biochemical sensors for medicine,food safety and environmental monitoring.However,there is little research on aptamer-target binding mechanisms,which limits their application and development.Computational simulation has gained much attention for revealing aptamer-target binding mechanisms at the atomic level.This work summarizes the main simulation methods used in the mechanistic analysis of aptamer-target complexes,the characteristics of binding between aptamers and different targets(metal ions,small organic molecules,biomacromolecules,cells,bacteria and viruses),the types of aptamer-target interactions and the factors influencing their strength.It provides a reference for further use of simulations in understanding aptamer-target binding mechanisms.
基金supported by the National Natural Science Foundation of China(22022402,21974051 for L.Zhang and 22004037,21635003,21827814,and 21811540027 for Y.Tian)the Innovation Program of Shanghai Municipal Education Commission(201701070005E00020)for Y.Tian.
文摘Potassium ion(K^(+))is widely involved in several physiopathological processes,and its abnormal changes are closely related to the occurrence of brain diseases of cerebral ischemia.In vivo acquirement of K^(+)variation is significant to understand the roles of K^(+)playing in brain functions.A microelectrode based on single-stranded DNA aptamers was developed for highly selective detection of K^(+)in brain,in which the aptamer probes were designed to contain an aptamer part for specific recognition of K^(+),an alkynyl group used for stable confinement of aptamer probe on the gold surface,and an electrochemical redox active ferrocene group to generate current response signal.The response range of the microelectrodes could be rationally tuned by varying the chain length of the aptamer probe.The optimized electrode,LAC,displayed high selectivity for in vivo detection of K^(+),and suitable linear range from 10 mmol·L-1e10 mmol·L-1,which could fulfill the requirement of K^(+)detection in brain.Eventually,the microelectrodes were successfully applied for the detection of K^(+)in the living mouse brains followed by hypoxic.
