Gossypium hirsutum L., one of the twocultivated tetraploid species in cotton,ischaracterized by its high yield and wideadaptation,while G.barbadense L.,anothercultivated one,by its super fiber properties.Substitution ...Gossypium hirsutum L., one of the twocultivated tetraploid species in cotton,ischaracterized by its high yield and wideadaptation,while G.barbadense L.,anothercultivated one,by its super fiber properties.Substitution line in which one pair of展开更多
Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cyt...Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.展开更多
While Upland cotton(Gossypium hirsutum L.) represents 95% of the world production,its genetic improvement is hindered by the shortage of effective genomic tools and resources.The
Restriction enzyme digestion of total genomic DNA of two chro-mosome type Paragonimus westermani showed the presence of homologus highlyrepeated DNA in diploid and triploid forms. Southern blot analysis providedfurthe...Restriction enzyme digestion of total genomic DNA of two chro-mosome type Paragonimus westermani showed the presence of homologus highlyrepeated DNA in diploid and triploid forms. Southern blot analysis providedfurther evidence that the distribution of restriction enzyme sites (with 3enzymes) on repeated DNA of both was similar. But with Pst Ⅰ、Dde Ⅰ、Hae Ⅲand Hpa Ⅱ. They revealed polymorphism which also occured individually ineach form tested separatedly with hybridization technique. Mutation of recog-nize sites by enzymes, which is resulted from the alternation of gene sequance,has occured during evolution. Additionally, it is probably possible, based on PstⅠand Dde Ⅰdigest pattern to distinguish between diploid and triploid Parag-onimus westermani and group the Paragonimus simply and rapidly.展开更多
The genetic base that cotton breeders commonly use to improve Upland cultivars is very narrow.The AD-genome species Gossypium barbadense,G.tomentosum,and G.mustelinum are part of
Engineered minichromosomes have been constructed as novel artificial chromosome platforms for future genetic engineering in maize.We demonstrated that minichromosomes could be created by telomere-mediated chromosomal ...Engineered minichromosomes have been constructed as novel artificial chromosome platforms for future genetic engineering in maize.We demonstrated that minichromosomes could be created by telomere-mediated chromosomal truncation of both normal A chromosomes and the supernumerary展开更多
The technology of cloning and transferring oflarge DNA fragments in plants is important forhigh-efficient identification of new genes andstudy of gene functions.Tranformation-competent artificial chromosome(TAC)vector...The technology of cloning and transferring oflarge DNA fragments in plants is important forhigh-efficient identification of new genes andstudy of gene functions.Tranformation-competent artificial chromosome(TAC)vectorsystem has been shown to be very useful forefficient gene isolation in Arobidopsis thaliana.In order to develop an efficient platform for展开更多
Multiple BAC-FISH is a powerful tool for modern cytogenetic researching in both animals and plants.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.
CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used ...CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used to screen polymorphism among two parents,TM-1 and CSB14Sh,展开更多
Ribes plants,like most of other fruit trees,are characterized by their large number but small size of somatic chromosomes.In the light of these special characteristics,we have examined several factorial combinations o...Ribes plants,like most of other fruit trees,are characterized by their large number but small size of somatic chromosomes.In the light of these special characteristics,we have examined several factorial combinations of material, pretreatment,slide preparing method and stain to identify the most promising techique for studying the chromosomes and further analyzing the karyotypes and chromosome banding of Ribes plants. The resuts indicated that the combination of root tip with pretreatment of 2 m molL-1 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3～5 hours at 14℃,pre-hypotonic trcarment of 0.07mol L-1 KCl for 30 min, fixation in Carnoy's fluid,hydrolysis in 5% cellulase and 5% pectinase mixture for 4～5 hours at 25℃, post-hypotonic treatment in distilled water for half an hour and staining in Giemsa could make the chromosome prepa ration superior to other treatment combinations.展开更多
文摘Gossypium hirsutum L., one of the twocultivated tetraploid species in cotton,ischaracterized by its high yield and wideadaptation,while G.barbadense L.,anothercultivated one,by its super fiber properties.Substitution line in which one pair of
基金National Natural Science Foundation of China(31530053)
文摘Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.
文摘While Upland cotton(Gossypium hirsutum L.) represents 95% of the world production,its genetic improvement is hindered by the shortage of effective genomic tools and resources.The
文摘Restriction enzyme digestion of total genomic DNA of two chro-mosome type Paragonimus westermani showed the presence of homologus highlyrepeated DNA in diploid and triploid forms. Southern blot analysis providedfurther evidence that the distribution of restriction enzyme sites (with 3enzymes) on repeated DNA of both was similar. But with Pst Ⅰ、Dde Ⅰ、Hae Ⅲand Hpa Ⅱ. They revealed polymorphism which also occured individually ineach form tested separatedly with hybridization technique. Mutation of recog-nize sites by enzymes, which is resulted from the alternation of gene sequance,has occured during evolution. Additionally, it is probably possible, based on PstⅠand Dde Ⅰdigest pattern to distinguish between diploid and triploid Parag-onimus westermani and group the Paragonimus simply and rapidly.
文摘The genetic base that cotton breeders commonly use to improve Upland cultivars is very narrow.The AD-genome species Gossypium barbadense,G.tomentosum,and G.mustelinum are part of
文摘Engineered minichromosomes have been constructed as novel artificial chromosome platforms for future genetic engineering in maize.We demonstrated that minichromosomes could be created by telomere-mediated chromosomal truncation of both normal A chromosomes and the supernumerary
文摘The technology of cloning and transferring oflarge DNA fragments in plants is important forhigh-efficient identification of new genes andstudy of gene functions.Tranformation-competent artificial chromosome(TAC)vectorsystem has been shown to be very useful forefficient gene isolation in Arobidopsis thaliana.In order to develop an efficient platform for
文摘Multiple BAC-FISH is a powerful tool for modern cytogenetic researching in both animals and plants.But in cotton,this technique is unavailable due to the high percentage of repetitive sequences.
文摘CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used to screen polymorphism among two parents,TM-1 and CSB14Sh,
文摘Ribes plants,like most of other fruit trees,are characterized by their large number but small size of somatic chromosomes.In the light of these special characteristics,we have examined several factorial combinations of material, pretreatment,slide preparing method and stain to identify the most promising techique for studying the chromosomes and further analyzing the karyotypes and chromosome banding of Ribes plants. The resuts indicated that the combination of root tip with pretreatment of 2 m molL-1 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3～5 hours at 14℃,pre-hypotonic trcarment of 0.07mol L-1 KCl for 30 min, fixation in Carnoy's fluid,hydrolysis in 5% cellulase and 5% pectinase mixture for 4～5 hours at 25℃, post-hypotonic treatment in distilled water for half an hour and staining in Giemsa could make the chromosome prepa ration superior to other treatment combinations.
