Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns ...Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was 25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure includedα-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca2+binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK)activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA)stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.展开更多
This study focused on elucidating the lignocellulose degradation mechanism of Pholiota adiposa strain YAHS,aiming to provide theoretical basis and microbial resources for straw biorefining.Using the aniline blue-guaia...This study focused on elucidating the lignocellulose degradation mechanism of Pholiota adiposa strain YAHS,aiming to provide theoretical basis and microbial resources for straw biorefining.Using the aniline blue-guaiacol plate screening method,11 fungal strains were isolated from the Loess Plateau of northern Shaanxi.The highly efficient degrading strain P.adiposa YAHS was identified through DNS-based enzyme activity assays for cellulase and ligninase,combined with ITS sequence analysis.Whole-genome sequencing was performed using a hybrid approach integrating Illumina NovaSeq and Nanopore MinION platforms.Transcriptome-wide differential gene expression analysis was conducted via DESeq2,and untargeted metabolomics was carried out using UPLC-QTOF-MS.Multi-omics data were integrated to dissect the degradation pathways.Results showed that the genome of P.adiposa YAHS is 55.2 Mb in size,encoding 719 carbohydrate-active enzymes(CAZymes),with glycoside hydrolases(GHs)accounting for 37.4%.Multi-omics analysis revealed that this strain degrades lignocellulose into carbohydrates such as monosaccharides,oligosaccharides,and sugar alcohols through key enzymatic genes(e.g.,exoglucanase,β-glucosidase,β-xylosidase,β-mannanase,monooxygenase)and metabolic pathways(e.g.,sucrose/starch metabolism,fructose/mannose metabolism,anthranilate degradation).We preliminarily elucidated the lignocellulose degradation mechanism of fungi in the genus Pholiota through integrated multi-omics analysis,revealed the critical roles of key cellulolytic enzymes in this process,and provided important microbial resources and theoretical support for the development of novel biorefining technologies.展开更多
Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'...Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'-bipyridine]were successfully synthesized by the volatilization of the solution at room temperature.The crystal structures of six complexes were determined by single-crystal X-ray diffraction technology.The results showed that the complexes all have a binuclear structure,and the structures contain free ethanol molecules.Moreover,the coordination number of the central metal of each structural unit is eight.Adjacent structural units interact with each other through hydrogen bonds and further expand to form 1D chain-like and 2D planar structures.After conducting a systematic study on the luminescence properties of complexes 1-4,their emission and excitation spectra were obtained.Experimental results indicated that the fluorescence lifetimes of complexes 2 and 3 were 0.807 and 0.845 ms,respectively.The emission spectral data of complexes 1-4 were imported into the CIE chromaticity coordinate system,and their corre sponding luminescent regions cover the yellow light,red light,green light,and orange-red light bands,respectively.Within the temperature range of 299.15-1300 K,the thermal decomposition processes of the six complexes were comprehensively analyzed by using TG-DSC/FTIR/MS technology.The hypothesis of the gradual loss of ligand groups during the decomposition process was verified by detecting the escaped gas,3D infrared spectroscopy,and ion fragment information detected by mass spectrometry.The specific decomposition path is as follows:firstly,free ethanol molecules and neutral ligands are removed,and finally,acidic ligands are released;the final product is the corresponding metal oxide.CCDC:2430420,1;2430422,2;2430419,3;2430424,4;2430421,5;2430423,6.展开更多
The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recogni...The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recognized to be limited at data boundaries and high frequencies,which can significantly reduce the signal-to-noise ratio(SNR).To solve this problem,a novel method synergistically integrating Principal Component Analysis(PCA)with SG filtering is proposed in this paper.This approach avoids the is-sue of excessive smoothing associated with larger window sizes.The proposed PCA-SG filtering algorithm was applied to a CO gas sensing system based on Cavity Ring-Down Spectroscopy(CRDS).The perform-ance of the PCA-SG filtering algorithm is demonstrated through comparison with Moving Average Filtering(MAF),Wavelet Transformation(WT),Kalman Filtering(KF),and the SG filter.The results demonstrate that the proposed algorithm exhibits superior noise reduction capabilities compared to the other algorithms evaluated.The SNR of the ring-down signal was improved from 11.8612 dB to 29.0913 dB,and the stand-ard deviation of the extracted ring-down time constant was reduced from 0.037μs to 0.018μs.These results confirm that the proposed PCA-SG filtering algorithm effectively improves the smoothness of the ring-down curve data,demonstrating its feasibility.展开更多
This study theoretically explored the dynamic response of the liquid-filled cylindrical shell structure experiencing internal explosion shock waves.It analyzed the radial deformation of the liquid-filled cylindrical s...This study theoretically explored the dynamic response of the liquid-filled cylindrical shell structure experiencing internal explosion shock waves.It analyzed the radial deformation of the liquid-filled cylindrical shell structure theoretically.It clarified the protection mechanism of the externally liquid-filled cylindrical shell structure.Based on the improved single-degree-of-freedom system theory,a theoretical model was established via load equivalence and simplification.The radial deformations of unfilled and externally liquid-filled cylindrical shells was investigated under internal explosion shock waves.The influencing factors for structural protection characteristics were explored considering impact load intensity,liquid layer thickness,structural specifications and dimensions,and material properties.The results showed that when the load peak value or the action time was fixed,the maximum radial deformation of the structure increased with the increased load-specific impulse.When the load-specific impulse was fixed,reducing the load peak or extending the loading time decreased the maximum radial deformation of the structure.The protection mechanism of the externally liquid-filled cylindrical shell structure was due to the liquid medium,which acted as an additional mass that con-strained the radial deformation of the structure.The change in liquid layer thickness altered the duration of the liquid's constraint on the radial deformation.The dynamic response of the externally liquid-filled cylindrical shell structure presented three deformation modes,which were determined by the liquid layer thickness,structural specifications,dimensions,and material properties.展开更多
The periodic fluctuations in water levels in reservoir areas subject slope rocks to both wet-dry cycles and cyclic shear stresses.Understanding the deterioration mechanisms of rocks under these conditions is crucial f...The periodic fluctuations in water levels in reservoir areas subject slope rocks to both wet-dry cycles and cyclic shear stresses.Understanding the deterioration mechanisms of rocks under these conditions is crucial for ensuring their long-term stability.In this study,limestone samples were collected from the site,then mechanical tests and acoustic emission(AE)were conducted.The multifractal detrended fluctuation analysis(MF-DFA)was used to analyze the AE time series of the samples.The results indicate that the primary causes of decreased shear strength are the shearing of micro-asperities on the sample surface under cyclic shear,and the erosion of soluble mineral crystals under wet-dry cycles.The MF-DFA spectra revealed that the frequency of frictional damage events exceeded that of micro-asperity shear failures.Wet-dry cycles were found to decrease shear strength primarily by facilitating the shearing of existing micro-asperities,while their capacity to generate new micro-asperities was weak.The MF-DFA characteristics with time of AE can serve as an early warning indicator of sample deformation and failure.Compared to existing methods,this approach exhibits better early warning performance.The findings provide valuable insights for the assessment and early warning of long-term stability of geotechnical bodies in reservoir areas.展开更多
Multichannel signals have the characteristics of information diversity and information consistency.To better explore and utilize the affinity relationship within multichannel signals,a new graph learning technique bas...Multichannel signals have the characteristics of information diversity and information consistency.To better explore and utilize the affinity relationship within multichannel signals,a new graph learning technique based on low rank tensor approximation is proposed for multichannel monitoring signal processing and utilization.Firstly,the affinity relationship of multichannel signals can be acquired based on the clustering results of each channel signal.Wherein an affinity tensor is constructed to integrate the diverse and consistent information of the clustering information among multichannel signals.Secondly,a low-rank tensor optimization model is built and the joint affinity matrix is optimized with the assistance of the strong confidence affinity matrix.Through solving the optimization model,the fused affinity relationship graph of multichannel signals can be obtained.Finally,the multichannel fused clustering results can be acquired though the updated joint affinity relationship graph.The multichannel signal utilization examples in health state assessment with public datasets and microwave detection with actual echoes verify the advantages and effectiveness of the proposed method.展开更多
With the improvement of the informatization and intelligence level of logistics equipment,the interactive and collaborative relationships between equipment entities become complex,and the uncertainty problems emerge i...With the improvement of the informatization and intelligence level of logistics equipment,the interactive and collaborative relationships between equipment entities become complex,and the uncertainty problems emerge in the equipment system-of-systems.Herein,a heterogeneous network model is built to describe logistics equipment system-of-systems,which considers the heterogeneity and complex connections of different logistics equipment nodes.Next,the topological structure properties of this model are analyzed.On this basis,the experiments on the logistics equipment system-of-systems under attack strategies including degree attacks,betweenness centrality attacks and random attacks are taken to assess the changes of structural invulnerability.Results show that the logistics equipment system-of-systems heterogeneous network has similar topological structure characteristics of typical complex networks,namely small-world effect and scale-free characteristics,indicating that the flow,sharing,and synchronization between logistics equipment entities in the network are relatively easy.Meantime,the key logistics equipment nodes with large values such as degree,closeness centrality,and betweenness centrality should be protected in the logistics equipment system-ofsystems heterogeneous network against deliberate attacks.The current work provides a perspective for demonstration and affords the theoretical support for development and decisionmaking of logistics equipment system-of-systems.展开更多
The remarkable mechanical and physical characteristics of functionally graded(FG)graphene origami(GOri)-enabled auxetic metamaterial(GOEAM)structures,including their high strength-to-weight ratio,tunable stiffness and...The remarkable mechanical and physical characteristics of functionally graded(FG)graphene origami(GOri)-enabled auxetic metamaterial(GOEAM)structures,including their high strength-to-weight ratio,tunable stiffness and strength,and negative Poisson's ratio(NPR),have demonstrated significant promise for a range of engineering applications.This paper aims to investigate the nonlinear free vibrations behaviors of FG-GOEAM non-uniform thickness skew-microplate with rectangular and elliptical planform and resting on Kerr-elastic foundation in thermal environment.Within the framework of the higher-order shear deformation theory(HSDT),von Kármán assumption,modified couple stress theory(MCST)and by employing Hamilton's principle the nonlinear governing equations of motion are established.By combining an iterative technique with a displacement control strategy,an isogeometric analysis(IGA)approach used to determine the nonlinearity in free vibration,as measured by the nonlinear frequency ratio associated with the center deflection amplitude.The effects of GOri distribution patterns,weight fraction,length-scale parameter,temperature difference,skew-angle,and micro-plate dimensions on the nonlinear free vibrations behaviors of the FG-GOEAM non-uniform thickness skew-microplate are revealed through a thorough parametric study.This result can be applied in studies on the design of micro-electro-mechanical devices operating in various complex environments and conditions.展开更多
Cascading failures pose a serious threat to the survivability of underwater unmanned swarm networks(UUSNs),significantly limiting their service ability in collaborative missions such as military reconnaissance and env...Cascading failures pose a serious threat to the survivability of underwater unmanned swarm networks(UUSNs),significantly limiting their service ability in collaborative missions such as military reconnaissance and environmental monitoring.Existing failure models primarily focus on power grids and traffic systems,and don't address the unique challenges of weak-communication UUSNs.In UUSNs,cascading failure present a complex and dynamic process driven by the coupling of unstable acoustic channels,passive node drift,adversarial attacks,and network heterogeneity.To address these challenges,a directed weighted graph model of UUSNs is first developed,in which node positions are updated according to ocean-current-driven drift and link weights reflect the probability of successful acoustic transmission.Building on this UUSNs graph model,a cascading failure model is proposed that integrates a normal-failure-recovery state-cycle mechanism,multiple attack strategies,and routingbased load redistribution.Finally,under a five-level connectivity UUSNs scheme,simulations are conducted to analyze how dynamic topology,network load,node recovery delay,and attack modes jointly affect network survivability.The main findings are:(1)moderate node drift can improve survivability by activating weak links;(2)based-energy routing(BER)outperform based-depth routing(BDR)in harsh conditions;(3)node self-recovery time is critical to network survivability;(4)traditional degree-based critical node metrics are inadequate for weak-communication UUSNs.These results provide a theoretical foundation for designing robust survivability mechanisms in weak-communication UUSNs.展开更多
This study aims to establish an integrated sensitivity analysis framework for optimization and design of the dynamic performance of mechanical systems such as tracked vehicles,by combining the direct differentiation m...This study aims to establish an integrated sensitivity analysis framework for optimization and design of the dynamic performance of mechanical systems such as tracked vehicles,by combining the direct differentiation method(DDM)with the linear multibody system transfer matrix method(linear MSTMM).The rigid-flexible coupled multibody system dynamics model of a tracked vehicle is established using the linear MSTMM and validated through the modal test.Building upon the existing DDM-based eigenvalue sensitivity analysis method within the linear MSTMM,the DDM is embedded into it to enable programmable and efficient computation of dynamic response sensitivities for mechanical systems.The proposed approach is used to quantitatively evaluate the sensitivities of both natural vibration characteristics(e.g.,natural frequencies and mode shapes)and transient dynamic responses of the tracked vehicle with respect to system parameters,successfully identifying critical structural parameters.Compared to conventional finite difference methods,the developed methodology eliminates sensitivity to perturbation step sizes.The contributions of this work lie in establishing a unified theoretical foundation and analysis framework for guiding dynamics optimization and design of mechanical systems,and extending the applicability of the linear MSTMM to sensitivity analysis of transient dynamic responses.展开更多
The combustion and detonation processes of energetic materials exhibit remarkable complexity and ultra-fast transient characteristics.While reactive molecular dynamics has been extensively employed to investigate the ...The combustion and detonation processes of energetic materials exhibit remarkable complexity and ultra-fast transient characteristics.While reactive molecular dynamics has been extensively employed to investigate the reaction dynamics of energetic materials,its utility is often constrained to capturing only fundamental reaction events and species information,thereby limiting mechanistic investigations of complex reaction pathways.To elucidate the topological features of energetic material reaction networks and identify critical reaction pathways with high fidelity,this study presents ReacNetwork-an advanced large-scale reaction network analysis methodology that synergistically integrates complex network theory with molecular simulation techniques.Specifically,we have developed a multi-dimensional feature screening protocol based on node centrality metrics and K-shell decomposition algorithms.Takingα-Hexahydro-1,3,5-trinitro-1,3,5-triazine(α-RDX)as the subject,we successfully constructed a comprehensive high-temperature thermal decomposition reaction network consisting of 1,134 distinct chemical species and 3,626 elementary reactions.Through systematic application of community detection algorithms and global topological feature extraction techniques,we achieved effective dimensionality reduction and successfully identified the dominant reaction pathway within theα-RDX thermal decomposition network.The computational results not only validate the well-established initial reaction mechanism dominated by N-NO2 homolytic bond cleavage,but also provide unprecedented visualization ofα-RDX framework ring-opening dynamics and subsequent radical chain propagation networks.展开更多
The multi-target assignment(MTA)problem,a crucial challenge in command control,mission planning,and a fundamental research focus in military operations,has garnered significant attention over the years.Extensively stu...The multi-target assignment(MTA)problem,a crucial challenge in command control,mission planning,and a fundamental research focus in military operations,has garnered significant attention over the years.Extensively studied across various domains such as land,sea,air,space,and electronics,the MTA problem has led to the emergence of numerous models and algorithms.To delve deeper into this field,this paper starts by conducting a bibliometric analysis on 463 Scopus database papers using CiteSpace software.The analysis includes examining keyword clustering,co-occurrence,and burst,with visual representations of the results.Following this,the paper provides an overview of current classification and modeling techniques for addressing the MTA problem,distinguishing between static multi-target assignment(SMTA)and dynamic multi-target assignment(DMTA).Subsequently,existing solution algorithms for the MTA problem are reviewed,generally falling into three categories:exact algorithms,heuristic algorithms,and machine learning algorithms.Finally,a development framework is proposed based on the"HIGH"model(high-speed,integrated,great,harmonious)to guide future research and intelligent weapon system development concerning the MTA problem.This framework emphasizes application scenarios,modeling mechanisms,solution algorithms,and system efficiency to offer a roadmap for future exploration in this area.展开更多
Anti-jamming performance evaluation has recently received significant attention. For Link-16, the anti-jamming performance evaluation and selection of the optimal anti-jamming technologies are urgent problems to be so...Anti-jamming performance evaluation has recently received significant attention. For Link-16, the anti-jamming performance evaluation and selection of the optimal anti-jamming technologies are urgent problems to be solved. A comprehensive evaluation method is proposed, which combines grey relational analysis (GRA) and cloud model, to evaluate the anti-jamming performances of Link-16. Firstly, on the basis of establishing the anti-jamming performance evaluation indicator system of Link-16, the linear combination of analytic hierarchy process(AHP) and entropy weight method (EWM) are used to calculate the combined weight. Secondly, the qualitative and quantitative concept transformation model, i.e., the cloud model, is introduced to evaluate the anti-jamming abilities of Link-16 under each jamming scheme. In addition, GRA calculates the correlation degree between evaluation indicators and the anti-jamming performance of Link-16, and assesses the best anti-jamming technology. Finally, simulation results prove that the proposed evaluation model can achieve the objective of feasible and practical evaluation, which opens up a novel way for the research of anti-jamming performance evaluations of Link-16.展开更多
The intersection is a widely used traffic line structure from the shallow tunnel to the deep roadway,and determining the subsidence hidden danger area of the roof is the key to its stability control.However,applying t...The intersection is a widely used traffic line structure from the shallow tunnel to the deep roadway,and determining the subsidence hidden danger area of the roof is the key to its stability control.However,applying traditional maximum equivalent span beam(MESB)theory to determine deformation range,peak point,and angle influence poses a challenge.Considering the overall structure of the intersection roof,the maximum equivalent triangular plate(METP)theory is proposed,and its geometric parameter calculation formula and deflection calculation formula are obtained.The application of the two theories in 18 models with different intersection angles,roadway types,and surrounding rock lithology is verified by numerical analysis.The results show that:1)The METP structure of the intersection roof established by the simulation results of each model successfully determined the location of the roof’s high displacement zone;2)The area comparison method of the METP theory can be reasonably explained:①The roof subsidence of the intersection decreases with the increase of the intersection angle;②The roof subsidence at the intersection of different roadway types has a rectangular type>arch type>circular type;③The roof subsidence of the intersection with weak surrounding rock is significantly larger than that of the intersection with hard surrounding rock.According to the application results of the two theories,the four advantages of the METP theory are compared and clarified in the basic assumptions,mechanical models,main viewpoints,and mechanism analysis.The large deformation inducement of the intersection roof is then explored.The J 2 peak area of the roof drives the large deformation of the area,the peak point of which is consistent with the center of gravity position of the METP.Furthermore,the change in the range of this peak is consistent with the change law of the METP’s area.Hence,this theory clarifies the large deformation area of the intersection roof,which provides a clear guiding basis for its initial support design,mid-term monitoring,and late local reinforcement.展开更多
Objective Recent studies have highlighted the critical role of NUDT19 in the initiation,progression,and prognosis of specific cancer types.However,its involvement in pan-cancer analysis has not been fully characterize...Objective Recent studies have highlighted the critical role of NUDT19 in the initiation,progression,and prognosis of specific cancer types.However,its involvement in pan-cancer analysis has not been fully characterized.This study aims to systematically explore the expression patterns,clinical significance,and immune-related functions of NUDT19 in various cancer types through multi-omics analysis,further revealing its potential role in cancer,particularly its functional and therapeutic target value in leukemia.Methods To achieve this goal,various bioinformatics approaches were employed to evaluate the expression patterns,clinical significance,and immune-related functions of NUDT19 in tumors and normal tissues.Additionally,we analyzed the mutation characteristics of NUDT19 and its relationship with epigenetic modifications.Using the single-cell analysis tool SingleCellBase,we explored the distribution of NUDT19 across different cell subpopulations in tumors.To validate these findings,qRT-PCR was used to measure NUDT19 expression levels in specific tumor cell lines,and we established acute myeloid leukemia(AML)cell lines(HL-60 and THP-1)to conduct NUDT19 knockdown and overexpression experiments,assessing its effects on leukemia cell proliferation,apoptosis,and invasion.Results Pan-cancer analysis revealed the dysregulated expression of NUDT19 across multiple cancer types,which was closely associated with poor prognosis,clinical staging,and diagnostic markers.Furthermore,NUDT19 was significantly correlated with tumor biomarkers,immune-related genes,and immune cell infiltration in different cancers.Mutation analysis showed that multiple mutations in NUDT19 were significantly associated with epigenetic changes.Single-cell analysis revealed the heterogeneity of NUDT19 expression in cancer cells,suggesting its potentially diverse functional roles in different cell subpopulations.qRT-PCR experiments confirmed the significant upregulation of NUDT19 in various tumor cell lines.In AML cell lines,NUDT19 knockdown led to reduced cell proliferation and invasion,with increased apoptosis,while NUDT19 overexpression significantly enhanced cell proliferation and invasion while reducing apoptosis.Conclusion This study demonstrates the diverse roles of NUDT19 in various cancer types,with a particularly prominent functional role in leukemia.NUDT19 is not only associated with tumor initiation and progression but may also influence cancer progression through the regulation of immune microenvironment and epigenetic mechanisms.Our research highlights the potential of NUDT19 as a therapeutic target,particularly for targeted therapies in malignancies such as leukemia,with significant clinical application prospects.展开更多
The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic an...The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic analysis of a WSC1 knockout(ΔWSC1)strain.The knockout of WSC1 significantly altered the gene expression profile in P.expansum,particularly for genes involved in cell wall integrity,signaling,stress response,and toxin production.The differential expression of these genes might make theΔWSC1 strain more vulnerable to environmental stress,while reducing the toxin production capacity,ultimately leading to a decrease in the pathogenicity.The transcriptomic analysis revealed that the expression of genes related to stress response signals,defense mechanisms and oxidative stress management changed when pear fruits were infected with theΔWSC1 strain.These changes may trigger a cascade of responses in pear fruits.In addition,compared with those infected with the wild-type strain,pear fruits infected with theΔWSC1 strain exhibited up-regulated expression of genes related to defense and oxidative stress.This study clarifies how the WSC1 gene influences P.expansum’s ability to infect pear fruits and how pear fruits respond to the infection.展开更多
High-precision e^(+)e^(-)→cc data(20 final states)from the BESⅢ and Belle at√s=3.75-4.7 GeV are analyzed with a semi three-body unitary coupled-channel model.After fitting the data,we extract J^(PC)=1^(--)charmoniu...High-precision e^(+)e^(-)→cc data(20 final states)from the BESⅢ and Belle at√s=3.75-4.7 GeV are analyzed with a semi three-body unitary coupled-channel model.After fitting the data,we extract J^(PC)=1^(--)charmonium poles from the obtained amplitudes.We find well-known states listed in the PDG(particle data group),and also several states near thresholds of open-charm channels.The compositeness analysis suggests that ψ(4040)could mainly consist of aD*D*--molecule component,rather than a conventionally accepted quark-model ψ(3S)state.Additionally,ψ(4230)and ψ(4360)might be substantial mixtures of D_(1)(2420)D,D_(1)(2420)D*,D*_(s)D*_(s),and cc components.展开更多
Restoration of phase aberrations is crucial for addressing atmospheric turbulence in light propagation.Traditional restoration algorithms based on Zernike polynomials(ZPs)often encounter challenges related to high com...Restoration of phase aberrations is crucial for addressing atmospheric turbulence in light propagation.Traditional restoration algorithms based on Zernike polynomials(ZPs)often encounter challenges related to high computational complexity and insufficient capture of high-frequency phase aberration components,so we proposed a Principal-Component-Analysis-based method for representing phase aberrations.This paper discusses the factors influencing the accuracy of restoration,mainly including the sample space size and the sampling interval of D/r_(0),on the basis of characterizing phase aberrations by Principal Components(PCs).The experimental results show that a larger D/r_(0)sampling interval can ensure the generalization ability and robustness of the principal components in the case of a limited amount of original data,which can help to achieve high-precision deployment of the model in practical applications quickly.In the environment with relatively strong turbulence in the test set of D/r_(0)=24,the use of 34 terms of PCs can improve the corrected Strehl ratio(SR)from 0.007 to 0.1585,while the Strehl ratio of the light spot after restoration using 34 terms of ZPs is only 0.0215,demonstrating almost no correction effect.The results indicate that PCs can serve as a better alternative in representing and restoring the characteristics of atmospheric turbulence induced phase aberrations.These findings pave the way to use PCs of phase aberrations with fewer terms than traditional ZPs to achieve data dimensionality reduction,and offer a reference to accelerate and stabilize the model and deep learning based adaptive optics correction.展开更多
In this paper,we develop a multi-scalar auxiliary variables(MSAV)scheme for the Cahn-Hilliard Magnetohydrodynamics system by introducing two scalar auxiliary variables(SAV).This scheme is linear,fully decoupled and un...In this paper,we develop a multi-scalar auxiliary variables(MSAV)scheme for the Cahn-Hilliard Magnetohydrodynamics system by introducing two scalar auxiliary variables(SAV).This scheme is linear,fully decoupled and unconditionally stable in energy.Subsequently,we provide a detailed implementation procedure for full decoupling.Thus,at each time step,only a series of linear differential equations with constant coefficients need to be solved.To validate the effectiveness of our approach,we conduct an error analysis for this first-order scheme.Finally,some numerical experiments are provided to verify the energy dissipation of the system and the convergence of the proposed approach.展开更多
基金Supported by the National Key R&D Program(2022YFF1300500)the Spring Goose Support Program(CYQN24018)the National Natural Science Foundation of China(32572123)。
文摘Dianthus spiculifolius Schur,as an emerging ornamental plant,has extensive applications and economic values.In this study,the DsCBL4 gene was successfully cloned,and its tissue-specific expression,expression patterns under various abiotic stresses,subcellular localization,and bioinformatics analysis of the encoded amino acid sequence were conducted.The results showed that the coding region of the DsCBL4 gene was 675 bp long,encoding 224 amino acids.It had high homology with the amino acids encoded by Amaranthus tricolor,Chenopodium quinoa and Spinacia oleracea.The predicted relative molecular mass of DsCBL4 was 25.61 ku,with an isoelectric point of 4.58,and it had phosphorylation sites,belonging to an unstable hydrophilic protein.Its secondary structure includedα-helices,irregular coils and extended chains.The tertiary structure prediction revealed that DsCBL4 had four EFhand calcium-binding domains necessary for Ca2+binding in plant calmodulin-like proteins and the FPSF motif for calcineurin B-like protein(CBL)-interacting protein kinase(CIPK)activation.The expression level of the DsCBL4 gene showed tissue specificity,with the highest expression in roots.It was induced by drought,low temperature,combined drought and low temperature,salt stress,nitrogen stress,phosphorus stress,calcium ion stress,high temperature stress,and abscisic acid(ABA)stress.Both transient infection in Nicotiana tabacum L.and stable expression in transgenic Arabidopsis thaliana showed that the DsCBL4 protein was localized to the cell membrane.These results suggested that DsCBL4 might be involved in the abiotic stress response of Dianthus spiculifolius through the calcium signaling pathway,providing a theoretical basis for understanding its molecular mechanism.This study provided an important reference for further exploring the role of the DsCBLs gene family in plant stress resistance.
文摘This study focused on elucidating the lignocellulose degradation mechanism of Pholiota adiposa strain YAHS,aiming to provide theoretical basis and microbial resources for straw biorefining.Using the aniline blue-guaiacol plate screening method,11 fungal strains were isolated from the Loess Plateau of northern Shaanxi.The highly efficient degrading strain P.adiposa YAHS was identified through DNS-based enzyme activity assays for cellulase and ligninase,combined with ITS sequence analysis.Whole-genome sequencing was performed using a hybrid approach integrating Illumina NovaSeq and Nanopore MinION platforms.Transcriptome-wide differential gene expression analysis was conducted via DESeq2,and untargeted metabolomics was carried out using UPLC-QTOF-MS.Multi-omics data were integrated to dissect the degradation pathways.Results showed that the genome of P.adiposa YAHS is 55.2 Mb in size,encoding 719 carbohydrate-active enzymes(CAZymes),with glycoside hydrolases(GHs)accounting for 37.4%.Multi-omics analysis revealed that this strain degrades lignocellulose into carbohydrates such as monosaccharides,oligosaccharides,and sugar alcohols through key enzymatic genes(e.g.,exoglucanase,β-glucosidase,β-xylosidase,β-mannanase,monooxygenase)and metabolic pathways(e.g.,sucrose/starch metabolism,fructose/mannose metabolism,anthranilate degradation).We preliminarily elucidated the lignocellulose degradation mechanism of fungi in the genus Pholiota through integrated multi-omics analysis,revealed the critical roles of key cellulolytic enzymes in this process,and provided important microbial resources and theoretical support for the development of novel biorefining technologies.
文摘Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'-bipyridine]were successfully synthesized by the volatilization of the solution at room temperature.The crystal structures of six complexes were determined by single-crystal X-ray diffraction technology.The results showed that the complexes all have a binuclear structure,and the structures contain free ethanol molecules.Moreover,the coordination number of the central metal of each structural unit is eight.Adjacent structural units interact with each other through hydrogen bonds and further expand to form 1D chain-like and 2D planar structures.After conducting a systematic study on the luminescence properties of complexes 1-4,their emission and excitation spectra were obtained.Experimental results indicated that the fluorescence lifetimes of complexes 2 and 3 were 0.807 and 0.845 ms,respectively.The emission spectral data of complexes 1-4 were imported into the CIE chromaticity coordinate system,and their corre sponding luminescent regions cover the yellow light,red light,green light,and orange-red light bands,respectively.Within the temperature range of 299.15-1300 K,the thermal decomposition processes of the six complexes were comprehensively analyzed by using TG-DSC/FTIR/MS technology.The hypothesis of the gradual loss of ligand groups during the decomposition process was verified by detecting the escaped gas,3D infrared spectroscopy,and ion fragment information detected by mass spectrometry.The specific decomposition path is as follows:firstly,free ethanol molecules and neutral ligands are removed,and finally,acidic ligands are released;the final product is the corresponding metal oxide.CCDC:2430420,1;2430422,2;2430419,3;2430424,4;2430421,5;2430423,6.
文摘The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recognized to be limited at data boundaries and high frequencies,which can significantly reduce the signal-to-noise ratio(SNR).To solve this problem,a novel method synergistically integrating Principal Component Analysis(PCA)with SG filtering is proposed in this paper.This approach avoids the is-sue of excessive smoothing associated with larger window sizes.The proposed PCA-SG filtering algorithm was applied to a CO gas sensing system based on Cavity Ring-Down Spectroscopy(CRDS).The perform-ance of the PCA-SG filtering algorithm is demonstrated through comparison with Moving Average Filtering(MAF),Wavelet Transformation(WT),Kalman Filtering(KF),and the SG filter.The results demonstrate that the proposed algorithm exhibits superior noise reduction capabilities compared to the other algorithms evaluated.The SNR of the ring-down signal was improved from 11.8612 dB to 29.0913 dB,and the stand-ard deviation of the extracted ring-down time constant was reduced from 0.037μs to 0.018μs.These results confirm that the proposed PCA-SG filtering algorithm effectively improves the smoothness of the ring-down curve data,demonstrating its feasibility.
基金support pro-vided by National Natural Science Foundation of China(Grant Nos.52371342 and 52271338).
文摘This study theoretically explored the dynamic response of the liquid-filled cylindrical shell structure experiencing internal explosion shock waves.It analyzed the radial deformation of the liquid-filled cylindrical shell structure theoretically.It clarified the protection mechanism of the externally liquid-filled cylindrical shell structure.Based on the improved single-degree-of-freedom system theory,a theoretical model was established via load equivalence and simplification.The radial deformations of unfilled and externally liquid-filled cylindrical shells was investigated under internal explosion shock waves.The influencing factors for structural protection characteristics were explored considering impact load intensity,liquid layer thickness,structural specifications and dimensions,and material properties.The results showed that when the load peak value or the action time was fixed,the maximum radial deformation of the structure increased with the increased load-specific impulse.When the load-specific impulse was fixed,reducing the load peak or extending the loading time decreased the maximum radial deformation of the structure.The protection mechanism of the externally liquid-filled cylindrical shell structure was due to the liquid medium,which acted as an additional mass that con-strained the radial deformation of the structure.The change in liquid layer thickness altered the duration of the liquid's constraint on the radial deformation.The dynamic response of the externally liquid-filled cylindrical shell structure presented three deformation modes,which were determined by the liquid layer thickness,structural specifications,dimensions,and material properties.
基金Project(CSTB2023NSCQ-MSX0864)supported by the Chongqing Science and Technology Commission,ChinaProjects(52209122,52478328)supported by the National Natural Science Foundation of ChinaProject(CSTB2024TIADGPX0058)supported by the Chongqing Technology Innovation and Application Development Special Project,China。
文摘The periodic fluctuations in water levels in reservoir areas subject slope rocks to both wet-dry cycles and cyclic shear stresses.Understanding the deterioration mechanisms of rocks under these conditions is crucial for ensuring their long-term stability.In this study,limestone samples were collected from the site,then mechanical tests and acoustic emission(AE)were conducted.The multifractal detrended fluctuation analysis(MF-DFA)was used to analyze the AE time series of the samples.The results indicate that the primary causes of decreased shear strength are the shearing of micro-asperities on the sample surface under cyclic shear,and the erosion of soluble mineral crystals under wet-dry cycles.The MF-DFA spectra revealed that the frequency of frictional damage events exceeded that of micro-asperity shear failures.Wet-dry cycles were found to decrease shear strength primarily by facilitating the shearing of existing micro-asperities,while their capacity to generate new micro-asperities was weak.The MF-DFA characteristics with time of AE can serve as an early warning indicator of sample deformation and failure.Compared to existing methods,this approach exhibits better early warning performance.The findings provide valuable insights for the assessment and early warning of long-term stability of geotechnical bodies in reservoir areas.
基金supported by Shanghai Aerospace Science and Technology Innovation Foundation(SAST2023-075)。
文摘Multichannel signals have the characteristics of information diversity and information consistency.To better explore and utilize the affinity relationship within multichannel signals,a new graph learning technique based on low rank tensor approximation is proposed for multichannel monitoring signal processing and utilization.Firstly,the affinity relationship of multichannel signals can be acquired based on the clustering results of each channel signal.Wherein an affinity tensor is constructed to integrate the diverse and consistent information of the clustering information among multichannel signals.Secondly,a low-rank tensor optimization model is built and the joint affinity matrix is optimized with the assistance of the strong confidence affinity matrix.Through solving the optimization model,the fused affinity relationship graph of multichannel signals can be obtained.Finally,the multichannel fused clustering results can be acquired though the updated joint affinity relationship graph.The multichannel signal utilization examples in health state assessment with public datasets and microwave detection with actual echoes verify the advantages and effectiveness of the proposed method.
文摘With the improvement of the informatization and intelligence level of logistics equipment,the interactive and collaborative relationships between equipment entities become complex,and the uncertainty problems emerge in the equipment system-of-systems.Herein,a heterogeneous network model is built to describe logistics equipment system-of-systems,which considers the heterogeneity and complex connections of different logistics equipment nodes.Next,the topological structure properties of this model are analyzed.On this basis,the experiments on the logistics equipment system-of-systems under attack strategies including degree attacks,betweenness centrality attacks and random attacks are taken to assess the changes of structural invulnerability.Results show that the logistics equipment system-of-systems heterogeneous network has similar topological structure characteristics of typical complex networks,namely small-world effect and scale-free characteristics,indicating that the flow,sharing,and synchronization between logistics equipment entities in the network are relatively easy.Meantime,the key logistics equipment nodes with large values such as degree,closeness centrality,and betweenness centrality should be protected in the logistics equipment system-ofsystems heterogeneous network against deliberate attacks.The current work provides a perspective for demonstration and affords the theoretical support for development and decisionmaking of logistics equipment system-of-systems.
文摘The remarkable mechanical and physical characteristics of functionally graded(FG)graphene origami(GOri)-enabled auxetic metamaterial(GOEAM)structures,including their high strength-to-weight ratio,tunable stiffness and strength,and negative Poisson's ratio(NPR),have demonstrated significant promise for a range of engineering applications.This paper aims to investigate the nonlinear free vibrations behaviors of FG-GOEAM non-uniform thickness skew-microplate with rectangular and elliptical planform and resting on Kerr-elastic foundation in thermal environment.Within the framework of the higher-order shear deformation theory(HSDT),von Kármán assumption,modified couple stress theory(MCST)and by employing Hamilton's principle the nonlinear governing equations of motion are established.By combining an iterative technique with a displacement control strategy,an isogeometric analysis(IGA)approach used to determine the nonlinearity in free vibration,as measured by the nonlinear frequency ratio associated with the center deflection amplitude.The effects of GOri distribution patterns,weight fraction,length-scale parameter,temperature difference,skew-angle,and micro-plate dimensions on the nonlinear free vibrations behaviors of the FG-GOEAM non-uniform thickness skew-microplate are revealed through a thorough parametric study.This result can be applied in studies on the design of micro-electro-mechanical devices operating in various complex environments and conditions.
基金supported in part by the National Natural Science Foundation of China(Key Program)under Grant No.62031021。
文摘Cascading failures pose a serious threat to the survivability of underwater unmanned swarm networks(UUSNs),significantly limiting their service ability in collaborative missions such as military reconnaissance and environmental monitoring.Existing failure models primarily focus on power grids and traffic systems,and don't address the unique challenges of weak-communication UUSNs.In UUSNs,cascading failure present a complex and dynamic process driven by the coupling of unstable acoustic channels,passive node drift,adversarial attacks,and network heterogeneity.To address these challenges,a directed weighted graph model of UUSNs is first developed,in which node positions are updated according to ocean-current-driven drift and link weights reflect the probability of successful acoustic transmission.Building on this UUSNs graph model,a cascading failure model is proposed that integrates a normal-failure-recovery state-cycle mechanism,multiple attack strategies,and routingbased load redistribution.Finally,under a five-level connectivity UUSNs scheme,simulations are conducted to analyze how dynamic topology,network load,node recovery delay,and attack modes jointly affect network survivability.The main findings are:(1)moderate node drift can improve survivability by activating weak links;(2)based-energy routing(BER)outperform based-depth routing(BDR)in harsh conditions;(3)node self-recovery time is critical to network survivability;(4)traditional degree-based critical node metrics are inadequate for weak-communication UUSNs.These results provide a theoretical foundation for designing robust survivability mechanisms in weak-communication UUSNs.
基金supported by the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20241443)the Jiangsu Funding Program for Excellent Postdoctoral Talent(Grant No.2024ZB072)the National Natural Science Foundation of China(Grant No.92266201).
文摘This study aims to establish an integrated sensitivity analysis framework for optimization and design of the dynamic performance of mechanical systems such as tracked vehicles,by combining the direct differentiation method(DDM)with the linear multibody system transfer matrix method(linear MSTMM).The rigid-flexible coupled multibody system dynamics model of a tracked vehicle is established using the linear MSTMM and validated through the modal test.Building upon the existing DDM-based eigenvalue sensitivity analysis method within the linear MSTMM,the DDM is embedded into it to enable programmable and efficient computation of dynamic response sensitivities for mechanical systems.The proposed approach is used to quantitatively evaluate the sensitivities of both natural vibration characteristics(e.g.,natural frequencies and mode shapes)and transient dynamic responses of the tracked vehicle with respect to system parameters,successfully identifying critical structural parameters.Compared to conventional finite difference methods,the developed methodology eliminates sensitivity to perturbation step sizes.The contributions of this work lie in establishing a unified theoretical foundation and analysis framework for guiding dynamics optimization and design of mechanical systems,and extending the applicability of the linear MSTMM to sensitivity analysis of transient dynamic responses.
基金support from the National Natural Science Foundation of China(Grant No.22275018).
文摘The combustion and detonation processes of energetic materials exhibit remarkable complexity and ultra-fast transient characteristics.While reactive molecular dynamics has been extensively employed to investigate the reaction dynamics of energetic materials,its utility is often constrained to capturing only fundamental reaction events and species information,thereby limiting mechanistic investigations of complex reaction pathways.To elucidate the topological features of energetic material reaction networks and identify critical reaction pathways with high fidelity,this study presents ReacNetwork-an advanced large-scale reaction network analysis methodology that synergistically integrates complex network theory with molecular simulation techniques.Specifically,we have developed a multi-dimensional feature screening protocol based on node centrality metrics and K-shell decomposition algorithms.Takingα-Hexahydro-1,3,5-trinitro-1,3,5-triazine(α-RDX)as the subject,we successfully constructed a comprehensive high-temperature thermal decomposition reaction network consisting of 1,134 distinct chemical species and 3,626 elementary reactions.Through systematic application of community detection algorithms and global topological feature extraction techniques,we achieved effective dimensionality reduction and successfully identified the dominant reaction pathway within theα-RDX thermal decomposition network.The computational results not only validate the well-established initial reaction mechanism dominated by N-NO2 homolytic bond cleavage,but also provide unprecedented visualization ofα-RDX framework ring-opening dynamics and subsequent radical chain propagation networks.
基金the financial support provided by the National Natural Science Foundation of China(NSFC)(Grant No.62173274)the National Key R&D Program of China(Grant No.2019YFA0405300)+4 种基金the Natural Science Foundation of Hunan Province of China(Grant No.2021JJ10045)the Practice and Innovation Funds for Graduate Students of Northwestern Polytechnical University(Grant No.PF2023046)the Open Research Subject of State Key Laboratory of Intelligent Game(Grant No.ZBKF-24-01)the Postdoctoral Fellowship Program of CPSF(No.GZB20240989)the China Postdoctoral Science Foundation(Grant No.2024M754304)。
文摘The multi-target assignment(MTA)problem,a crucial challenge in command control,mission planning,and a fundamental research focus in military operations,has garnered significant attention over the years.Extensively studied across various domains such as land,sea,air,space,and electronics,the MTA problem has led to the emergence of numerous models and algorithms.To delve deeper into this field,this paper starts by conducting a bibliometric analysis on 463 Scopus database papers using CiteSpace software.The analysis includes examining keyword clustering,co-occurrence,and burst,with visual representations of the results.Following this,the paper provides an overview of current classification and modeling techniques for addressing the MTA problem,distinguishing between static multi-target assignment(SMTA)and dynamic multi-target assignment(DMTA).Subsequently,existing solution algorithms for the MTA problem are reviewed,generally falling into three categories:exact algorithms,heuristic algorithms,and machine learning algorithms.Finally,a development framework is proposed based on the"HIGH"model(high-speed,integrated,great,harmonious)to guide future research and intelligent weapon system development concerning the MTA problem.This framework emphasizes application scenarios,modeling mechanisms,solution algorithms,and system efficiency to offer a roadmap for future exploration in this area.
基金Heilongjiang Provincial Natural Science Foundation of China (LH2021F009)。
文摘Anti-jamming performance evaluation has recently received significant attention. For Link-16, the anti-jamming performance evaluation and selection of the optimal anti-jamming technologies are urgent problems to be solved. A comprehensive evaluation method is proposed, which combines grey relational analysis (GRA) and cloud model, to evaluate the anti-jamming performances of Link-16. Firstly, on the basis of establishing the anti-jamming performance evaluation indicator system of Link-16, the linear combination of analytic hierarchy process(AHP) and entropy weight method (EWM) are used to calculate the combined weight. Secondly, the qualitative and quantitative concept transformation model, i.e., the cloud model, is introduced to evaluate the anti-jamming abilities of Link-16 under each jamming scheme. In addition, GRA calculates the correlation degree between evaluation indicators and the anti-jamming performance of Link-16, and assesses the best anti-jamming technology. Finally, simulation results prove that the proposed evaluation model can achieve the objective of feasible and practical evaluation, which opens up a novel way for the research of anti-jamming performance evaluations of Link-16.
基金Project(52204164)supported by the National Natural Science Foundation of ChinaProject(2021QNRC001)supported by the Young Elite Scientists Sponsorship Program by CAST,China。
文摘The intersection is a widely used traffic line structure from the shallow tunnel to the deep roadway,and determining the subsidence hidden danger area of the roof is the key to its stability control.However,applying traditional maximum equivalent span beam(MESB)theory to determine deformation range,peak point,and angle influence poses a challenge.Considering the overall structure of the intersection roof,the maximum equivalent triangular plate(METP)theory is proposed,and its geometric parameter calculation formula and deflection calculation formula are obtained.The application of the two theories in 18 models with different intersection angles,roadway types,and surrounding rock lithology is verified by numerical analysis.The results show that:1)The METP structure of the intersection roof established by the simulation results of each model successfully determined the location of the roof’s high displacement zone;2)The area comparison method of the METP theory can be reasonably explained:①The roof subsidence of the intersection decreases with the increase of the intersection angle;②The roof subsidence at the intersection of different roadway types has a rectangular type>arch type>circular type;③The roof subsidence of the intersection with weak surrounding rock is significantly larger than that of the intersection with hard surrounding rock.According to the application results of the two theories,the four advantages of the METP theory are compared and clarified in the basic assumptions,mechanical models,main viewpoints,and mechanism analysis.The large deformation inducement of the intersection roof is then explored.The J 2 peak area of the roof drives the large deformation of the area,the peak point of which is consistent with the center of gravity position of the METP.Furthermore,the change in the range of this peak is consistent with the change law of the METP’s area.Hence,this theory clarifies the large deformation area of the intersection roof,which provides a clear guiding basis for its initial support design,mid-term monitoring,and late local reinforcement.
文摘Objective Recent studies have highlighted the critical role of NUDT19 in the initiation,progression,and prognosis of specific cancer types.However,its involvement in pan-cancer analysis has not been fully characterized.This study aims to systematically explore the expression patterns,clinical significance,and immune-related functions of NUDT19 in various cancer types through multi-omics analysis,further revealing its potential role in cancer,particularly its functional and therapeutic target value in leukemia.Methods To achieve this goal,various bioinformatics approaches were employed to evaluate the expression patterns,clinical significance,and immune-related functions of NUDT19 in tumors and normal tissues.Additionally,we analyzed the mutation characteristics of NUDT19 and its relationship with epigenetic modifications.Using the single-cell analysis tool SingleCellBase,we explored the distribution of NUDT19 across different cell subpopulations in tumors.To validate these findings,qRT-PCR was used to measure NUDT19 expression levels in specific tumor cell lines,and we established acute myeloid leukemia(AML)cell lines(HL-60 and THP-1)to conduct NUDT19 knockdown and overexpression experiments,assessing its effects on leukemia cell proliferation,apoptosis,and invasion.Results Pan-cancer analysis revealed the dysregulated expression of NUDT19 across multiple cancer types,which was closely associated with poor prognosis,clinical staging,and diagnostic markers.Furthermore,NUDT19 was significantly correlated with tumor biomarkers,immune-related genes,and immune cell infiltration in different cancers.Mutation analysis showed that multiple mutations in NUDT19 were significantly associated with epigenetic changes.Single-cell analysis revealed the heterogeneity of NUDT19 expression in cancer cells,suggesting its potentially diverse functional roles in different cell subpopulations.qRT-PCR experiments confirmed the significant upregulation of NUDT19 in various tumor cell lines.In AML cell lines,NUDT19 knockdown led to reduced cell proliferation and invasion,with increased apoptosis,while NUDT19 overexpression significantly enhanced cell proliferation and invasion while reducing apoptosis.Conclusion This study demonstrates the diverse roles of NUDT19 in various cancer types,with a particularly prominent functional role in leukemia.NUDT19 is not only associated with tumor initiation and progression but may also influence cancer progression through the regulation of immune microenvironment and epigenetic mechanisms.Our research highlights the potential of NUDT19 as a therapeutic target,particularly for targeted therapies in malignancies such as leukemia,with significant clinical application prospects.
文摘The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic analysis of a WSC1 knockout(ΔWSC1)strain.The knockout of WSC1 significantly altered the gene expression profile in P.expansum,particularly for genes involved in cell wall integrity,signaling,stress response,and toxin production.The differential expression of these genes might make theΔWSC1 strain more vulnerable to environmental stress,while reducing the toxin production capacity,ultimately leading to a decrease in the pathogenicity.The transcriptomic analysis revealed that the expression of genes related to stress response signals,defense mechanisms and oxidative stress management changed when pear fruits were infected with theΔWSC1 strain.These changes may trigger a cascade of responses in pear fruits.In addition,compared with those infected with the wild-type strain,pear fruits infected with theΔWSC1 strain exhibited up-regulated expression of genes related to defense and oxidative stress.This study clarifies how the WSC1 gene influences P.expansum’s ability to infect pear fruits and how pear fruits respond to the infection.
文摘High-precision e^(+)e^(-)→cc data(20 final states)from the BESⅢ and Belle at√s=3.75-4.7 GeV are analyzed with a semi three-body unitary coupled-channel model.After fitting the data,we extract J^(PC)=1^(--)charmonium poles from the obtained amplitudes.We find well-known states listed in the PDG(particle data group),and also several states near thresholds of open-charm channels.The compositeness analysis suggests that ψ(4040)could mainly consist of aD*D*--molecule component,rather than a conventionally accepted quark-model ψ(3S)state.Additionally,ψ(4230)and ψ(4360)might be substantial mixtures of D_(1)(2420)D,D_(1)(2420)D*,D*_(s)D*_(s),and cc components.
文摘Restoration of phase aberrations is crucial for addressing atmospheric turbulence in light propagation.Traditional restoration algorithms based on Zernike polynomials(ZPs)often encounter challenges related to high computational complexity and insufficient capture of high-frequency phase aberration components,so we proposed a Principal-Component-Analysis-based method for representing phase aberrations.This paper discusses the factors influencing the accuracy of restoration,mainly including the sample space size and the sampling interval of D/r_(0),on the basis of characterizing phase aberrations by Principal Components(PCs).The experimental results show that a larger D/r_(0)sampling interval can ensure the generalization ability and robustness of the principal components in the case of a limited amount of original data,which can help to achieve high-precision deployment of the model in practical applications quickly.In the environment with relatively strong turbulence in the test set of D/r_(0)=24,the use of 34 terms of PCs can improve the corrected Strehl ratio(SR)from 0.007 to 0.1585,while the Strehl ratio of the light spot after restoration using 34 terms of ZPs is only 0.0215,demonstrating almost no correction effect.The results indicate that PCs can serve as a better alternative in representing and restoring the characteristics of atmospheric turbulence induced phase aberrations.These findings pave the way to use PCs of phase aberrations with fewer terms than traditional ZPs to achieve data dimensionality reduction,and offer a reference to accelerate and stabilize the model and deep learning based adaptive optics correction.
基金Research Project Supported by Shanxi Scholarship Council of China(2021-029)International Cooperation Base and Platform Project of Shanxi Province(202104041101019)Basic Research Plan of Shanxi Province(202203021211129)。
文摘In this paper,we develop a multi-scalar auxiliary variables(MSAV)scheme for the Cahn-Hilliard Magnetohydrodynamics system by introducing two scalar auxiliary variables(SAV).This scheme is linear,fully decoupled and unconditionally stable in energy.Subsequently,we provide a detailed implementation procedure for full decoupling.Thus,at each time step,only a series of linear differential equations with constant coefficients need to be solved.To validate the effectiveness of our approach,we conduct an error analysis for this first-order scheme.Finally,some numerical experiments are provided to verify the energy dissipation of the system and the convergence of the proposed approach.
