A culture technique had been developed by which the normal mor-phological integrity of the porcine thyroid tissue was well preserved. Few ultra-strvctural changes following TSE stimulation could be detected compared w...A culture technique had been developed by which the normal mor-phological integrity of the porcine thyroid tissue was well preserved. Few ultra-strvctural changes following TSE stimulation could be detected compared withthe control group. Parafollicular cells were detected after being cultured for 96h in the presence of TSH. For the first time, we used scanning electron micro-scope to study the ultrastructural changes of organ cultured thyroid gland.展开更多
40 thyroid specimens from Tru-cut needle biopsy and surgerywere analyzed by APAAP immunohistochemical technique.It was shown thatthe expression of HLA-DR antigen on thyroid follicular cells (TFC) wasinvolved in almost...40 thyroid specimens from Tru-cut needle biopsy and surgerywere analyzed by APAAP immunohistochemical technique.It was shown thatthe expression of HLA-DR antigen on thyroid follicular cells (TFC) wasinvolved in almost every patient.The degree of DR expression was signifi-cantly higher in autoimmune thyroiditis (AIT) than in Graves’ disease (GD)and in nontoxic goiter (NTG),The level of DR expression on TFC not onlycorrelated markedly with degree of MNC infiltration,but more so with degreeof DR expression on intrathyroidal infiltrates,suggesting that aberrant DR ex-pression in vivo may be related to the activation of intrathyroidal T cells.展开更多
The functional activity and morphological integrity of the porcine thyroid tissue could be maintained up to 96 hrs with organ culture technique. Tg,T<sub>4</sub> and T<sub>3</sub> showed a almo...The functional activity and morphological integrity of the porcine thyroid tissue could be maintained up to 96 hrs with organ culture technique. Tg,T<sub>4</sub> and T<sub>3</sub> showed a almost similar localization and immunohistochemical staining pattern: diffuse staining of follicular lumen （L）, granular staining of follicular border（G）, and foamy staining of follicular border （F）. The significant differences between the thyrotropinstimulated and non-stimulated groups were observed. The peripheral vacuoles of colloid were increased （P【0.05） in the former. Dominant staining patterns were G and F in the former. This simple organ culture system is a useful model for studying the functional aspects such as hormone biosynthesis in thyroid gland.展开更多
In order to interpret pathologic mechanism of free radicals and thyroid hormone metabolism in cattle iodine and selenium deficiency, 20 heads of yellow cattle were selected from NiuJia town, Wu Chang City, Heilongjian...In order to interpret pathologic mechanism of free radicals and thyroid hormone metabolism in cattle iodine and selenium deficiency, 20 heads of yellow cattle were selected from NiuJia town, Wu Chang City, Heilongjiang Province, China, and were randomly devided into 4 groups with 5 for each. ① supplemented with 0.7 mg·kg -1 iodine(potassium iodine), ② supplemented with 0.2 mg·kg -1 selenium (sodium selenite), ③ supplemented with 0.7 mg·kg -1 iodine(potassium Iodine) plus 0.2 mg·kg -1 selenium (sodium selenite) per day for 30 days, respectively. ④control group. The whole blood glutathione peroxidase (GSH-px) and catalase (CAT) activities, free radicals (FR) concentration, erythrocyte superoxide dismutase (SOD) activity and molonaldehyde (MDA) concentration, the serum triiodothyronine (T 3)、thyroxine (T 4) and thyrotropin (TSH) were determined on the day of supplementation day-0 and day-30, respectively. It was showed that average iodine concentration in drinking water and diet were 3.82 μg·L -1 and 0.285mg·kg -1 , respectively, Diet selenium was 0.0498mg·kg -1 , Serum protein bound iodine(PBI) was 7.02 μg·100 mL, Blood selenium was 0.14 mg·L -1 , the schoolchildren′s goiter was 21.8%. It indicated that iodine and selenium were deficient in the investigated area. Whole blood GSH-px and CAT activities and serum T 3 concentration were significantly higher (P< 0.01 ), FR concentration and serum TSH were significantly lower(P<0.01) in the first three groups than that of the control, T 4 content in the first group was higher(P<0.05), T 4 was also higher (P>0.05) in the second group. and lower in the third group. The SOD and MDA in erythrocyte were not changed during the experimental period, The results also showed that GSH-px and CAT activities were increased, and FR decreased oberviously in the third group more than the other two groups, In addition, Thyroid hormone metabolism was more coincided with the physiologic status in the third group. the iodine and the selenium played an important role in the pathologic process of free radical metabolic disorder. selenium not only had the function of antioxidation by derectly scavenging free radicals, but also affected through GSH-px and CAT activities. iodine deficiency results in the Goiter, selenium deficiency aggravated iodine deficiency, Iodine and the selenium were dependent and restrained each other in the course of free radicals and thyroid hormone metabolism with a synergistic state.展开更多
Introduction:Antithyroid drugs are widely used in the treatment of Graves' disease(GD), but the relapse rate is very high after therapy withdrawal.We evaluated the reduction effects of intrathyroid injection of de...Introduction:Antithyroid drugs are widely used in the treatment of Graves' disease(GD), but the relapse rate is very high after therapy withdrawal.We evaluated the reduction effects of intrathyroid injection of dexamethasone(IID) on the relapse rate of hyperthyroidism in patients with newly diagnosed GD.Patients and Methods:A total of 191 patients with GD completed the study.After 6 months of treatment with methimazole(MMI), the patients were randomly assigned to receive either MMI(96 patients) alone or MMI combined with IID(MMI + IID;95 patients) treatment for 3 months, followed by continuing a dose of MMI that would maintain euthyroidism for the next 9 months in all of the patients.After withdrawal of the medical therapy, patients were followed for 24 months, and the relapse rate of hyperthyroidism was evaluated.Results:No statistical difference was observed in the levels of serum FT4, TSH, or TSH receptor antibodies(TR-Ab), the thyroid volume, or the TR-Ab positive rate between the two groups at month 6.After the next 3 months of treatment with MMI + IID or MMI alone, the levels of TSH increased significantly, and the levels of serum TR-Ab, the TR-Ab positive rate, and thyroid volume decreased significantly in the MMI + IID group compared with the MMI group.Seven patients(7.4%) experienced a relapse of overt hyperthyroidism in the MMI + IID group and 49 patients(51%) in MMI group during the 2-yr follow-up period(P < 0.001).Conclusions:MMI + IID treatment is helpful to prevent relapse of hyperthyroidism in GD after medical therapy withdrawal.展开更多
文摘A culture technique had been developed by which the normal mor-phological integrity of the porcine thyroid tissue was well preserved. Few ultra-strvctural changes following TSE stimulation could be detected compared withthe control group. Parafollicular cells were detected after being cultured for 96h in the presence of TSH. For the first time, we used scanning electron micro-scope to study the ultrastructural changes of organ cultured thyroid gland.
文摘40 thyroid specimens from Tru-cut needle biopsy and surgerywere analyzed by APAAP immunohistochemical technique.It was shown thatthe expression of HLA-DR antigen on thyroid follicular cells (TFC) wasinvolved in almost every patient.The degree of DR expression was signifi-cantly higher in autoimmune thyroiditis (AIT) than in Graves’ disease (GD)and in nontoxic goiter (NTG),The level of DR expression on TFC not onlycorrelated markedly with degree of MNC infiltration,but more so with degreeof DR expression on intrathyroidal infiltrates,suggesting that aberrant DR ex-pression in vivo may be related to the activation of intrathyroidal T cells.
文摘The functional activity and morphological integrity of the porcine thyroid tissue could be maintained up to 96 hrs with organ culture technique. Tg,T<sub>4</sub> and T<sub>3</sub> showed a almost similar localization and immunohistochemical staining pattern: diffuse staining of follicular lumen （L）, granular staining of follicular border（G）, and foamy staining of follicular border （F）. The significant differences between the thyrotropinstimulated and non-stimulated groups were observed. The peripheral vacuoles of colloid were increased （P【0.05） in the former. Dominant staining patterns were G and F in the former. This simple organ culture system is a useful model for studying the functional aspects such as hormone biosynthesis in thyroid gland.
文摘In order to interpret pathologic mechanism of free radicals and thyroid hormone metabolism in cattle iodine and selenium deficiency, 20 heads of yellow cattle were selected from NiuJia town, Wu Chang City, Heilongjiang Province, China, and were randomly devided into 4 groups with 5 for each. ① supplemented with 0.7 mg·kg -1 iodine(potassium iodine), ② supplemented with 0.2 mg·kg -1 selenium (sodium selenite), ③ supplemented with 0.7 mg·kg -1 iodine(potassium Iodine) plus 0.2 mg·kg -1 selenium (sodium selenite) per day for 30 days, respectively. ④control group. The whole blood glutathione peroxidase (GSH-px) and catalase (CAT) activities, free radicals (FR) concentration, erythrocyte superoxide dismutase (SOD) activity and molonaldehyde (MDA) concentration, the serum triiodothyronine (T 3)、thyroxine (T 4) and thyrotropin (TSH) were determined on the day of supplementation day-0 and day-30, respectively. It was showed that average iodine concentration in drinking water and diet were 3.82 μg·L -1 and 0.285mg·kg -1 , respectively, Diet selenium was 0.0498mg·kg -1 , Serum protein bound iodine(PBI) was 7.02 μg·100 mL, Blood selenium was 0.14 mg·L -1 , the schoolchildren′s goiter was 21.8%. It indicated that iodine and selenium were deficient in the investigated area. Whole blood GSH-px and CAT activities and serum T 3 concentration were significantly higher (P< 0.01 ), FR concentration and serum TSH were significantly lower(P<0.01) in the first three groups than that of the control, T 4 content in the first group was higher(P<0.05), T 4 was also higher (P>0.05) in the second group. and lower in the third group. The SOD and MDA in erythrocyte were not changed during the experimental period, The results also showed that GSH-px and CAT activities were increased, and FR decreased oberviously in the third group more than the other two groups, In addition, Thyroid hormone metabolism was more coincided with the physiologic status in the third group. the iodine and the selenium played an important role in the pathologic process of free radical metabolic disorder. selenium not only had the function of antioxidation by derectly scavenging free radicals, but also affected through GSH-px and CAT activities. iodine deficiency results in the Goiter, selenium deficiency aggravated iodine deficiency, Iodine and the selenium were dependent and restrained each other in the course of free radicals and thyroid hormone metabolism with a synergistic state.
文摘Introduction:Antithyroid drugs are widely used in the treatment of Graves' disease(GD), but the relapse rate is very high after therapy withdrawal.We evaluated the reduction effects of intrathyroid injection of dexamethasone(IID) on the relapse rate of hyperthyroidism in patients with newly diagnosed GD.Patients and Methods:A total of 191 patients with GD completed the study.After 6 months of treatment with methimazole(MMI), the patients were randomly assigned to receive either MMI(96 patients) alone or MMI combined with IID(MMI + IID;95 patients) treatment for 3 months, followed by continuing a dose of MMI that would maintain euthyroidism for the next 9 months in all of the patients.After withdrawal of the medical therapy, patients were followed for 24 months, and the relapse rate of hyperthyroidism was evaluated.Results:No statistical difference was observed in the levels of serum FT4, TSH, or TSH receptor antibodies(TR-Ab), the thyroid volume, or the TR-Ab positive rate between the two groups at month 6.After the next 3 months of treatment with MMI + IID or MMI alone, the levels of TSH increased significantly, and the levels of serum TR-Ab, the TR-Ab positive rate, and thyroid volume decreased significantly in the MMI + IID group compared with the MMI group.Seven patients(7.4%) experienced a relapse of overt hyperthyroidism in the MMI + IID group and 49 patients(51%) in MMI group during the 2-yr follow-up period(P < 0.001).Conclusions:MMI + IID treatment is helpful to prevent relapse of hyperthyroidism in GD after medical therapy withdrawal.
