[目的]为雪莲果的开发利用提供理论依据。[方法]称取10.0 g雪莲果,洗净、切碎,加入40%的乙醇180 m l,回流提取其中的绿原酸,自然冷却后过滤。波谱定性检验选择适宜的测定波长,以95%乙醇为参比溶液,用配制好的一系列不同浓度的绿原酸标...[目的]为雪莲果的开发利用提供理论依据。[方法]称取10.0 g雪莲果,洗净、切碎,加入40%的乙醇180 m l,回流提取其中的绿原酸,自然冷却后过滤。波谱定性检验选择适宜的测定波长,以95%乙醇为参比溶液,用配制好的一系列不同浓度的绿原酸标准溶液绘制标准曲线,测定提取液的吸光度,计算滤液中绿原酸的含量。[结果]绿原酸标准品和雪莲果样品提取液在330 nm处有特征吸收峰,故选定330 nm为最大吸收波长,测定雪莲果样品液的吸光度。雪莲果中绿原酸的平均吸光度值为1.105,含量为0.052%。采用分光光度法测得雪莲果中绿原酸的RSD值为3.07%,平均回收率为105.85%。[结论]雪莲果中绿原酸的含量为0.052%。展开更多
AIM: Smallanthus sonchifolius (Yacon) has been used as a food and folk medicine in the Andes region. It was screened the pharmacological effects of a 50% ethanol extract of Yacon on spermatogenesis in rats. This study...AIM: Smallanthus sonchifolius (Yacon) has been used as a food and folk medicine in the Andes region. It was screened the pharmacological effects of a 50% ethanol extract of Yacon on spermatogenesis in rats. This study is carried out to evaluate the possibility of using Yacon tubers extracts as a fertility agent to treat infertility caused by spermatogenic disorders. METHODS: The dried, pulverized Yacon tubers were extracted with 50% ethanol at 50 ℃ for 5 h under sonication. The Yacon extracts were analyzed by using HPLC system on a Kromasil C18 column (250 mm×4.6 mm). The Yacon tubers extracts, chlorogenic acid and ferulic acid were administrated to rats once daily by oral gavage. The number of sperm heads in the testis was assessed by counting the number of sperm heads using slight modification of the procedure reported by Toth et al (1989). RESULTS: After administration of chlorogenic acid and Yacon tuber extracts to rat for 5 weeks, the number of sperm in epididymis were increased by 20.33% and 34.48%, respectively. The ferulic acid which was reported as a metabolite of chlorogenic acid and constituent of Yacon to estimate the spermatogenic activity was also administered. After administration of ferulic acid and Yacon tubers extracts, the sperm number were increased by 37.87% and 43.33%, respectively. Spermatogenic effects of chlorogenic acid and ferulic acid were 0.59 times and 0.87 times of Yacon tubers extracts, respectively. CONCLUSION: The spermatogenic effect of Yacon tubers extracts may result from the combined effect of chlorogenic acid or its analogues and their metabolites.展开更多
文摘AIM: Smallanthus sonchifolius (Yacon) has been used as a food and folk medicine in the Andes region. It was screened the pharmacological effects of a 50% ethanol extract of Yacon on spermatogenesis in rats. This study is carried out to evaluate the possibility of using Yacon tubers extracts as a fertility agent to treat infertility caused by spermatogenic disorders. METHODS: The dried, pulverized Yacon tubers were extracted with 50% ethanol at 50 ℃ for 5 h under sonication. The Yacon extracts were analyzed by using HPLC system on a Kromasil C18 column (250 mm×4.6 mm). The Yacon tubers extracts, chlorogenic acid and ferulic acid were administrated to rats once daily by oral gavage. The number of sperm heads in the testis was assessed by counting the number of sperm heads using slight modification of the procedure reported by Toth et al (1989). RESULTS: After administration of chlorogenic acid and Yacon tuber extracts to rat for 5 weeks, the number of sperm in epididymis were increased by 20.33% and 34.48%, respectively. The ferulic acid which was reported as a metabolite of chlorogenic acid and constituent of Yacon to estimate the spermatogenic activity was also administered. After administration of ferulic acid and Yacon tubers extracts, the sperm number were increased by 37.87% and 43.33%, respectively. Spermatogenic effects of chlorogenic acid and ferulic acid were 0.59 times and 0.87 times of Yacon tubers extracts, respectively. CONCLUSION: The spermatogenic effect of Yacon tubers extracts may result from the combined effect of chlorogenic acid or its analogues and their metabolites.
