摘要
建立了同时测定亚贡(Smallanthus sonchifolius)叶中亚贡二萜酸A和C含量的方法.采用高效液相色谱-质谱法,多反应监测(MRM)模式,大黄素为内标,色谱柱:UltimateTM XB-C18(150mm×4.6mm,5μm);流动相:V(甲醇)∶V(0.5%醋酸水溶液)=90∶10;流速:1.0mL/min;柱温:25℃.亚贡二萜酸A和C的线性范围均为0.2～10.0μg/mL(r≥0.999),平均加样回收率(n=6)分别为96.6%和92.4%,相对标准偏差(RSD)分别为1.9%和3.2%.实验数据表明,该方法操作简单、准确,且重复性好,可用于亚贡叶的质量控制.
A new fast and sensitive high-performance liquid chromatography-electrospray ionization tandem mass Spectrometry （HPLC-ESI-MS/MS） method was developed and validated for smaditerpenic acids A and C in leaves of Smallanthus sonchijolius using emodin as internal standard （IS）. The calibration curves of smaditerpenic acids A and C were investigated with a good linear relationship within the rangs of 0.2-10.0 μg/mL （r≥0. 999） ,and the recoveries （n=6） were 96.6% and 92.4% with RSD of 1.9% and 3.2%, respectively. The analytes were detected in negative ion mode using multiple reaction monitoring （MRM）. The method was validated and the specificity, linearity, lower limit of quantitation （LLOQ）, precision, accuracy, recoveries and stability were deter mined.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第1期72-76,共5页
Journal of Xiamen University：Natural Science
基金
福建省自然科学基金项目(2009J01192)
厦门市重大科技计划项目(3502Z20100006)
作者简介
通信作者：wuzhen@xmu．edu．cn
