Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation techni...Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation technique. The biological analysis results showed that population doubling time (PDT) of cells was approximately 24 h, diploid account for 90%～96% in chromosome. Confirmed by isoenzyme analysis,this cell line had no cross-contamination.The test results of the bacterium, fungi, virus, mycoplasma were negative. The transfection rate was high, and the extro-gene can be effectively expressed. Every indexes of the cell bank met all the standard quality controls of ATCC. It had not only preserved the germplasm resources of this important Small Tail Han Sheep on the cell level but also provided valuable material for the research of genome, postgenome and somacloning. Meanwhile, the setting-up of this technological platform will offer technology and theory for storing the cell genetic resources of other animals and poultry.展开更多
以视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因为候选基因,采用PCR-SSCP技术分析了RBP4基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、萨福克羊)中的单核苷酸多态性,同时研究这个基因对小尾寒羊高...以视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因为候选基因,采用PCR-SSCP技术分析了RBP4基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、萨福克羊)中的单核苷酸多态性,同时研究这个基因对小尾寒羊高繁殖力的影响。结果表明:RBP4基因扩增片段在4个绵羊品种中存在PCR-SSCP多态性。BB基因型只出现在高繁殖力绵羊品种中,而低繁殖力绵羊品种则没有BB基因型;AB基因型频率随着绵羊繁殖力的降低而升高;AA基因型只出现在小尾寒羊、多赛特羊中。BB基因型小尾寒羊产羔数分别比AA和AB基因型多0.52只(P<0.05)和0.67只(P<0.05),AA和AB基因型小尾寒羊产羔数没有显著差异(P>0.05)。本研究检测的绵羊RBP4基因座位与小尾寒羊高繁殖力相关。展开更多
文摘Taking Small Tail Han Sheeps ear margin as the study materials, we succeeded in establishing a fibroblast cell bank which content of samples was 52 by the method of explant culture directly and cryopreservation technique. The biological analysis results showed that population doubling time (PDT) of cells was approximately 24 h, diploid account for 90%～96% in chromosome. Confirmed by isoenzyme analysis,this cell line had no cross-contamination.The test results of the bacterium, fungi, virus, mycoplasma were negative. The transfection rate was high, and the extro-gene can be effectively expressed. Every indexes of the cell bank met all the standard quality controls of ATCC. It had not only preserved the germplasm resources of this important Small Tail Han Sheep on the cell level but also provided valuable material for the research of genome, postgenome and somacloning. Meanwhile, the setting-up of this technological platform will offer technology and theory for storing the cell genetic resources of other animals and poultry.
文摘以视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因为候选基因,采用PCR-SSCP技术分析了RBP4基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、萨福克羊)中的单核苷酸多态性,同时研究这个基因对小尾寒羊高繁殖力的影响。结果表明:RBP4基因扩增片段在4个绵羊品种中存在PCR-SSCP多态性。BB基因型只出现在高繁殖力绵羊品种中,而低繁殖力绵羊品种则没有BB基因型;AB基因型频率随着绵羊繁殖力的降低而升高;AA基因型只出现在小尾寒羊、多赛特羊中。BB基因型小尾寒羊产羔数分别比AA和AB基因型多0.52只(P<0.05)和0.67只(P<0.05),AA和AB基因型小尾寒羊产羔数没有显著差异(P>0.05)。本研究检测的绵羊RBP4基因座位与小尾寒羊高繁殖力相关。
