摘要
试验通过对小尾寒羊与巴美肉羊背最长肌进行转录组分析,旨在挖掘影响两种绵羊肉质性状的关键基因。分别选取小尾寒羊和巴美肉羊各3只,利用转录组高通量测序(RNA-Seq)技术对其背最长肌转录组文库进行测序,利用生物信息学对测序结果进行分析,筛选影响两者肉质相关的候选基因。结果发现,6个样品测序数据经质量控制后共得到120Gb的有效数据,共筛选出333个差异基因,其中上调基因有82个,下调基因有251个。GO功能富集与KEGG Pathway显著性富集分析结果共筛选了6个与肉质性状相关的候选基因:PDK4、MYF6、PPARGC1A、SLCO4A1、FABP4、LEP。试验通过对比小尾寒羊与巴美肉羊背最长肌转录组数据,筛选影响肉质性状的候选基因,丰富了绵羊基因组信息,为进一步阐述不同品种绵羊肉质性状分子机理奠定了基础。
In order to obtain the genes related to meat quality traits,the transcriptome of longissimus dorsi of 3 Small-tail Han sheep and 3 Bamei mutton sheep were analyzed using RNA-Seq technology.The sequencing data was analyzed by bioinformatics methods then obtained the interrelated candidate genes which affect the meat quality traits.After quality control,120 Gb clean data were obtained from 6 libraries.The differential expression gene were 333,which the expression of 82 genes was higher and 251 genes was lower.The results showed that 6 candidate genes（PDK4,MYF6,PPARGC1 A,SLCO4 A1,FABP4 and LEP）related to meat quality traits were screened by GO functional enrichment and KEGG Pathway analysis.Through comparison of the longissimus dorsi transcriptome data of Small-tail Han sheep and Bamei mutton sheep,we obtained the genes of meat quality traits,and enriched the information of sheep genome.This results laid a foundation for further elaboration of the molecular mechanism of meat quality traits in different sheep breeds.
出处
《中国畜牧兽医》
CAS
北大核心
2018年第1期122-130,共9页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31360542)
内蒙古自治区农牧业科学院青年创新基金(2015QNJJM04)
国家肉羊产业技术体系建设项目(CARS-39)
关键词
RNA-SEQ
肉质性状
转录组
小尾寒羊
巴美肉羊
背最长肌
RNA-Seq
meat quality trait
transcriptome
Small-tail Han sheep
13amei muttonsheep
longissimus dorsi
作者简介
王位（1988-），男，内蒙古包头人，硕士生，研究方向：动物遗传育种与繁殖暨绵羊肉质特色基因挖掘，E-mail：ww282459824@126．com;通信作者：刘永斌（1977-），男，内蒙古呼和浩特人，博士，研究员，研究方向：动物遗传育种与繁殖，E—mail：ybliu117@126．com;张文广（1973-），男，内蒙古呼和浩特人，教授，博士生导师，研究方向：数量基因组学暨性状遗传机理，E-mail：atcgnmbi@aliyun．com
