In this paper, a new partial transmit sequence（PTS）scheme with low computational complexity is proposed for the problems of high computational complexity in the conventional PTS method. By analyzing the relationship...In this paper, a new partial transmit sequence（PTS）scheme with low computational complexity is proposed for the problems of high computational complexity in the conventional PTS method. By analyzing the relationship of candidate sequences in the PTS method under the interleaved partition method, it has been discovered that some candidate sequences generated by phase factor sequences have the same peak average power ratio（PAPR）. Hence, phase factor sequences can be optimized to reduce their searching times. Then, the computational process of generating candidate sequences can be simplified by improving the utilization of data and minimizing the calculations of complex multiplication. The performance analysis shows that, compared with the conventional PTS scheme, the proposed approach significantly decreases the computational complexity and has no loss of PAPR performance.展开更多
We first design a discrete hyperchaotic system via piece-wise linear state feedback. The states of the closed loop system are locally expanding in two directions but absolutely bounded on the whole, which implies hype...We first design a discrete hyperchaotic system via piece-wise linear state feedback. The states of the closed loop system are locally expanding in two directions but absolutely bounded on the whole, which implies hyperchaos. Then, we use three suchlike hyperchaotic systems with different feedback gain matrices to design a pseudo-random sequence generator (PRSG). Through a threshold function, three sub-sequences generated from the output of piecewise linear functions are changed into 0-1 sequences. Then, followed by XOR operation, an unpredictable pseudo-random sequence (PRS) is ultimately obtained. The analysis and simulation results indicate that the PRS, generated with hyperchaotic systems, has desirable statistical features.展开更多
The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an enviro...The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an environment shift from circum\|shelf margin carbonate platform to foreslope, then to deep continental slope [1] . Correspondingly, the sediments change in vertical succession from quartz sandstone, via carbonate grainstone to dark shale intercalated with lithic sandstone of gravity flow. From the Tithonian to Valanginian, eight 3rd\|order sequences have been recognized [1] with clearly delineated sequence boundaries and reasonable biostratigraphic controls of ammonites. From the angle of sequence stratigraphy and sedimentary cycles, the important change happened at ca. 138Ma [2] of the Lower Tithonian (about 120m above Aulacosphinctoides hybonotum zone), where a waved subaerial erosion surface occurs in massive bioclastic grainstone. The boundary at ca. 134 5Ma (latest Tithonian, about 60m below the FAD of \%Berriasella cf. jacobi [3] ) comes to the next. The third one is at ca 132Ma (some 170m above the FAD of Berriasella cf. jacobi [3] , but about 60m below the FAD of Berriasella cf. grandis [3] ). In terms of sea\|level change, the sequence boundary at 134 5Ma is probably the most distinctive one, above which some 60m gravel\|bearing massive medium\|grained quartz sandstone was laid down and obviously truncates the underlying strata. In the dark shale below the sandstone, abundant in ammonites, such as Virgatosphinctes, Aulacosphinctes, Haplophylloceras and Gymnosphinctes are recorded [3] . The quartz sandstone itself does not bear identifiable fossils. Above the quartz sandstone, the strata mainly consists of dark silty shale up to 180m thick, with an prominent marine transgression at the base. Just above the first flooding surface (FFS), quite a number of ammonites such as \%Berriasella\% cf. \%jacobi, Blanofordiceras, Hymalayaites\% and \%Pseudosubplanites \%are documented [3] . The third sequence, which is delineated by submarine truncation of slope fan complexes at bottom, is also characterized by dark shale and silts, yielding ammonites \%Berriasella \%cf. \%grandis\% and B. cf. \%berthei.\%>From the viewpoint of sequence stratigraphy, we herein suggest to take the FFS in the second sequence as the J/K boundary. The sequence boundary below it can be used as a nice auxiliary marker, which represents a large sea\|level fall, and can be correlated widely in the world. From the angle of paleontology, the FFS is also ideal for the boundary, where the most important faunal turnover happened. Theoretically, taking FFS as a chronostratigraphic boundary has more advantages than using sequence boundary.展开更多
The optimal design of training sequences for channel estimation in multiple-input multiple-output （MIMO） systems under spatially correlated fading is considered. The channel is assumed to be a block-fading model wit...The optimal design of training sequences for channel estimation in multiple-input multiple-output （MIMO） systems under spatially correlated fading is considered. The channel is assumed to be a block-fading model with spatial correlation known at both the transmitter and the receiver. To minimize the channel estimation error, optimal training sequences are designed to exploit full information of the spatial correlation under the criterion of minimum mean square error （MMSE）. It is investigated that the spatial correlation is helpful to decrease the estimation error and the proposed training sequences have good performance via simulations.展开更多
In this paper, the temporal different characteristics between the target and background pixels are used to detect dim moving targets in the slow-evolving complex background. A local and global variance filter on tempo...In this paper, the temporal different characteristics between the target and background pixels are used to detect dim moving targets in the slow-evolving complex background. A local and global variance filter on temporal profiles is presented that addresses the temporal characteristics of the target and background pixels to eliminate the large variation of background temporal profiles. Firstly, the temporal behaviors of different types of image pixels of practical infrared scenes are analyzed.Then, the new local and global variance filter is proposed. The baseline of the fluctuation level of background temporal profiles is obtained by using the local and global variance filter. The height of the target pulse signal is extracted by subtracting the baseline from the original temporal profiles. Finally, a new target detection criterion is designed. The proposed method is applied to detect dim and small targets in practical infrared sequence images. The experimental results show that the proposed algorithm has good detection performance for dim moving small targets in the complex background.展开更多
The involvement of small RNAs in cotton fiber development is under explored.The objective of this work was to directly clone,annotate,and analyze small RNAs of developing ovules to reveal
The perfect sequences are so ideal that all out-of-phase autocorrelation coefficients are zero, and if the sequences are used as the coding modulating signal for the phase-modulated radar, there will be no interferenc...The perfect sequences are so ideal that all out-of-phase autocorrelation coefficients are zero, and if the sequences are used as the coding modulating signal for the phase-modulated radar, there will be no interference of side lobes theoretically. However, it has been proved that there are no binary perfect sequences of period 4 〈 n ≤ 12100. Hence, the almost perfect sequences with all out-of-phase autocorrelation coefficients as zero except one are of great practice in engineering. Currently, the cyclic difference set is one of most effective tools to analyze the binary sequences with perfect periodic autocorrelation function. In this article, two characteristic formulas corresponding to the autocorrelation and symmetric structure of almost perfect sequences are calculated respectively. All almost perfect sequences with period n, which is a multiple of 4, can be figured out by the two formulas. Several almost perfect sequences with different periods have been hunted by the program based on the two formulas and then applied to the simulation program and practical application for ionospheric sounding.展开更多
Cyclotomic sequences have good cryptographic properties and are closely related to difference sets.This paper proposes a new class of binary generalized cyclotomic sequences of order two and length pqr.Its linear comp...Cyclotomic sequences have good cryptographic properties and are closely related to difference sets.This paper proposes a new class of binary generalized cyclotomic sequences of order two and length pqr.Its linear complexity,minimal polynomial,and autocorrelation are investigated.The results show that these sequences have a large linear complexity when 2∈D1,which means they can resist the Berlekamp-Massey attack.Furthermore,the autocorrelation values are close to 0 with a probability of approximately 1?1/r.Therefore,when r is a big prime,the new sequence has a good autocorrelation.展开更多
Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study...Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study aims to identify potential pathogenetic gene mutations in patients with sporadic KC in the Han Chinese population.Methods:Twenty-five patients with primary KC as well as 50 unrelated population matched healthy controls,were included in this study to identify potential pathogenic gene mutations among sporadic KC patients in the Han Chinese population.Sanger sequencing and whole-exome sequencing(WES)were used to analyze mutations in the zinc finger protein 469(ZNF469)gene.Bioinformatics analysis was conducted to explore the potential role of ZNF469 in KC pathogenesis.Results:Five novel heterozygous missense variants were identified in KC patients.Among them,2 compound heterozygous variants,c.8986G>C(p.E2996Q)with c.11765A>C(p.D3922A),and c.4423C>G(p.L1475V)with c.10633G>A(p.G3545R),were determined to be possible pathogenic factors for KC.Conclusion:Mutations in the ZNF469 gene may contribute to the development of KC in the Han Chinese population.These mutation sites may provide valuable information for future genetic screening of KC patients and their families.展开更多
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ...【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.展开更多
In complex environments such as high dynamics and weak signals,a satellite signal compensation method based on prefabricated trajectory assistance and an improved adaptive Kalman filter is proposed for a 155 mm differ...In complex environments such as high dynamics and weak signals,a satellite signal compensation method based on prefabricated trajectory assistance and an improved adaptive Kalman filter is proposed for a 155 mm differential rotating rear-body control-guided projectile to address the situation of satellite signal flickering and loss in projectile navigation systems due to environmental limitations.First,establish the system state and measurement equation when receiving satellite signals normally.Second,a seven-degree-of-freedom external ballistic model is constructed,and the ideal trajectory output from the ballistic model is used to provide the virtual motion state of the projectile,which is input into a filter as a substitute observation when satellite signals are lost.Finally,an adaptive Kalman filter(AKF)is designed,the proposed adaptive Kalman filter can accurately adjust the estimation error covariance matrix and Kalman gain in real-time based on information covariance mismatch.The simulation results show that compared to the classical Kalman filter,it can reduce the average positioning error by more than 38.21%in the case of short-term and full-range loss of satellite signals,providing a new idea for the integrated navigation of projectiles with incomplete information under the condition of satellite signal loss.展开更多
Single-cell RNA sequencing(scRNA-seq)is one of the most advanced sequencing technologies for studying transcriptome landscape at the single-cell revolution.It provides numerous advantages over traditional RNA-seq.Sinc...Single-cell RNA sequencing(scRNA-seq)is one of the most advanced sequencing technologies for studying transcriptome landscape at the single-cell revolution.It provides numerous advantages over traditional RNA-seq.Since it was first used to profile single-cell transcriptome in plants in 2019,it has been extensively employed to perform different research in plants.Recently,scRNA-seq was also quickly adopted by the cotton research community to solve lots of scientific questions which have been never solved.In this comment,we highlighted the significant progress in employing scRNA-seq to cotton genetic and genomic study and its future potential applications.展开更多
12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight...12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.展开更多
The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many o...The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.展开更多
The genomic fusions of the anaplastic lymphoma kinase(ALK)gene have been widely recognized as effective therapeutic targets for non-small cell lung carcinoma(NSCLC).The Second Xiangya Hospital of Central South Univers...The genomic fusions of the anaplastic lymphoma kinase(ALK)gene have been widely recognized as effective therapeutic targets for non-small cell lung carcinoma(NSCLC).The Second Xiangya Hospital of Central South University has treated 2 NSCLC patients with 2 distinct novel ALK gene fusions.Case 1 was a 55-year-old male with a solid nodule located in the right hilar lobe on enhanced CT scan.Case 2 was a 47-year-old female with enhanced CT showing involvement of the left upper lobe of lung.Histopathological examination of tumor tissues confirmed lung adenocarcinoma in both cases.Immunohistochemical(IHC)staining demonstrated positivity for thyroid transcription factor 1(TTF-1)and ALK-D5F3 in tumor cells,while negativity for P40.The next-generation sequencing(NGS)tests identified a PNPT1-ALK(Exon22:Exon20)fusion variant in case 1 and a TCEAL2-ALK(Exon3:Exon19)fusion variant in case 2.The TCEAL2-ALK fusion was further confirmed by amplification refractory mutation system(ARMS)-PCR at the mRNA level.Both patients were treated with oral alectinib at a dosage of 600 mg twice daily.The tumors in both patients were significantly decreased after alectinib treatment,achieving partial response.At the time of submission,there was an absence of disease progression and the progression-free survival(PFS)had surpassed 1 year.It offered compelling evidences that the individuals with NSCLC and harboring either a PNPT1-ALK(Exon22:Exon20)fusion or a TCEAL2-ALK(Exon3:Exon19)fusion,experience favorable therapeutic outcomes through the administration of alectinib.This study expands the known ALK fusion variants database and supports the precision treatment of NSCLC using ALK tyrosine kinase inhibitors(TKIs).展开更多
基金supported by the National Natural Science Foundation of China(6167309361370152)the Science and Technology Project of Shenyang(F16-205-1-01)
文摘In this paper, a new partial transmit sequence（PTS）scheme with low computational complexity is proposed for the problems of high computational complexity in the conventional PTS method. By analyzing the relationship of candidate sequences in the PTS method under the interleaved partition method, it has been discovered that some candidate sequences generated by phase factor sequences have the same peak average power ratio（PAPR）. Hence, phase factor sequences can be optimized to reduce their searching times. Then, the computational process of generating candidate sequences can be simplified by improving the utilization of data and minimizing the calculations of complex multiplication. The performance analysis shows that, compared with the conventional PTS scheme, the proposed approach significantly decreases the computational complexity and has no loss of PAPR performance.
基金This project was supported by the National Natural Science Foundation of China (69874025).
文摘We first design a discrete hyperchaotic system via piece-wise linear state feedback. The states of the closed loop system are locally expanding in two directions but absolutely bounded on the whole, which implies hyperchaos. Then, we use three suchlike hyperchaotic systems with different feedback gain matrices to design a pseudo-random sequence generator (PRSG). Through a threshold function, three sub-sequences generated from the output of piecewise linear functions are changed into 0-1 sequences. Then, followed by XOR operation, an unpredictable pseudo-random sequence (PRS) is ultimately obtained. The analysis and simulation results indicate that the PRS, generated with hyperchaotic systems, has desirable statistical features.
基金theNationalNaturalScienceFoundationofChina (No ..4982 5 10 2 )
文摘The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an environment shift from circum\|shelf margin carbonate platform to foreslope, then to deep continental slope [1] . Correspondingly, the sediments change in vertical succession from quartz sandstone, via carbonate grainstone to dark shale intercalated with lithic sandstone of gravity flow. From the Tithonian to Valanginian, eight 3rd\|order sequences have been recognized [1] with clearly delineated sequence boundaries and reasonable biostratigraphic controls of ammonites. From the angle of sequence stratigraphy and sedimentary cycles, the important change happened at ca. 138Ma [2] of the Lower Tithonian (about 120m above Aulacosphinctoides hybonotum zone), where a waved subaerial erosion surface occurs in massive bioclastic grainstone. The boundary at ca. 134 5Ma (latest Tithonian, about 60m below the FAD of \%Berriasella cf. jacobi [3] ) comes to the next. The third one is at ca 132Ma (some 170m above the FAD of Berriasella cf. jacobi [3] , but about 60m below the FAD of Berriasella cf. grandis [3] ). In terms of sea\|level change, the sequence boundary at 134 5Ma is probably the most distinctive one, above which some 60m gravel\|bearing massive medium\|grained quartz sandstone was laid down and obviously truncates the underlying strata. In the dark shale below the sandstone, abundant in ammonites, such as Virgatosphinctes, Aulacosphinctes, Haplophylloceras and Gymnosphinctes are recorded [3] . The quartz sandstone itself does not bear identifiable fossils. Above the quartz sandstone, the strata mainly consists of dark silty shale up to 180m thick, with an prominent marine transgression at the base. Just above the first flooding surface (FFS), quite a number of ammonites such as \%Berriasella\% cf. \%jacobi, Blanofordiceras, Hymalayaites\% and \%Pseudosubplanites \%are documented [3] . The third sequence, which is delineated by submarine truncation of slope fan complexes at bottom, is also characterized by dark shale and silts, yielding ammonites \%Berriasella \%cf. \%grandis\% and B. cf. \%berthei.\%>From the viewpoint of sequence stratigraphy, we herein suggest to take the FFS in the second sequence as the J/K boundary. The sequence boundary below it can be used as a nice auxiliary marker, which represents a large sea\|level fall, and can be correlated widely in the world. From the angle of paleontology, the FFS is also ideal for the boundary, where the most important faunal turnover happened. Theoretically, taking FFS as a chronostratigraphic boundary has more advantages than using sequence boundary.
基金the National Science Foundation for Distinguished Young Scholars (60725105)the SixthProject of the Key Project of National Nature Science Foundation of China (60496316)+2 种基金the National "863" Project (2007AA012288)the National Nature Science Foundation of China (60572146)the "111" Project (B08038).
文摘The optimal design of training sequences for channel estimation in multiple-input multiple-output （MIMO） systems under spatially correlated fading is considered. The channel is assumed to be a block-fading model with spatial correlation known at both the transmitter and the receiver. To minimize the channel estimation error, optimal training sequences are designed to exploit full information of the spatial correlation under the criterion of minimum mean square error （MMSE）. It is investigated that the spatial correlation is helpful to decrease the estimation error and the proposed training sequences have good performance via simulations.
基金National Natural Science Foundation of China(61774120)
文摘In this paper, the temporal different characteristics between the target and background pixels are used to detect dim moving targets in the slow-evolving complex background. A local and global variance filter on temporal profiles is presented that addresses the temporal characteristics of the target and background pixels to eliminate the large variation of background temporal profiles. Firstly, the temporal behaviors of different types of image pixels of practical infrared scenes are analyzed.Then, the new local and global variance filter is proposed. The baseline of the fluctuation level of background temporal profiles is obtained by using the local and global variance filter. The height of the target pulse signal is extracted by subtracting the baseline from the original temporal profiles. Finally, a new target detection criterion is designed. The proposed method is applied to detect dim and small targets in practical infrared sequence images. The experimental results show that the proposed algorithm has good detection performance for dim moving small targets in the complex background.
文摘The involvement of small RNAs in cotton fiber development is under explored.The objective of this work was to directly clone,annotate,and analyze small RNAs of developing ovules to reveal
基金This poject was supported by the National Natural Science Foundation of China (40474066).
文摘The perfect sequences are so ideal that all out-of-phase autocorrelation coefficients are zero, and if the sequences are used as the coding modulating signal for the phase-modulated radar, there will be no interference of side lobes theoretically. However, it has been proved that there are no binary perfect sequences of period 4 〈 n ≤ 12100. Hence, the almost perfect sequences with all out-of-phase autocorrelation coefficients as zero except one are of great practice in engineering. Currently, the cyclic difference set is one of most effective tools to analyze the binary sequences with perfect periodic autocorrelation function. In this article, two characteristic formulas corresponding to the autocorrelation and symmetric structure of almost perfect sequences are calculated respectively. All almost perfect sequences with period n, which is a multiple of 4, can be figured out by the two formulas. Several almost perfect sequences with different periods have been hunted by the program based on the two formulas and then applied to the simulation program and practical application for ionospheric sounding.
基金supported by the National Key Research and Development Program of China(2016YFB0800601)the Natural Science Foundation of China(61303217+3 种基金61502372)the Fundamental Research Funds for the Central Universities(JB140115)the Natural Science Foundation of Shaanxi Province(2013JQ80022014JQ8313)
文摘Cyclotomic sequences have good cryptographic properties and are closely related to difference sets.This paper proposes a new class of binary generalized cyclotomic sequences of order two and length pqr.Its linear complexity,minimal polynomial,and autocorrelation are investigated.The results show that these sequences have a large linear complexity when 2∈D1,which means they can resist the Berlekamp-Massey attack.Furthermore,the autocorrelation values are close to 0 with a probability of approximately 1?1/r.Therefore,when r is a big prime,the new sequence has a good autocorrelation.
基金supported by the National Natural Science Foundation(82271057)the Natural Science Foundation of Hunan Province(2023JJ30818),China。
文摘Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study aims to identify potential pathogenetic gene mutations in patients with sporadic KC in the Han Chinese population.Methods:Twenty-five patients with primary KC as well as 50 unrelated population matched healthy controls,were included in this study to identify potential pathogenic gene mutations among sporadic KC patients in the Han Chinese population.Sanger sequencing and whole-exome sequencing(WES)were used to analyze mutations in the zinc finger protein 469(ZNF469)gene.Bioinformatics analysis was conducted to explore the potential role of ZNF469 in KC pathogenesis.Results:Five novel heterozygous missense variants were identified in KC patients.Among them,2 compound heterozygous variants,c.8986G>C(p.E2996Q)with c.11765A>C(p.D3922A),and c.4423C>G(p.L1475V)with c.10633G>A(p.G3545R),were determined to be possible pathogenic factors for KC.Conclusion:Mutations in the ZNF469 gene may contribute to the development of KC in the Han Chinese population.These mutation sites may provide valuable information for future genetic screening of KC patients and their families.
文摘【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.
基金funded by the National Natural Science Foundation of China (Grant No. 62471048)Open Fund Project of Beijing Key Laboratory of High Dynamic Navigation TechnologyKey Laboratory Fund Project of Modern Measurement and Control Technology, Ministry of Education
文摘In complex environments such as high dynamics and weak signals,a satellite signal compensation method based on prefabricated trajectory assistance and an improved adaptive Kalman filter is proposed for a 155 mm differential rotating rear-body control-guided projectile to address the situation of satellite signal flickering and loss in projectile navigation systems due to environmental limitations.First,establish the system state and measurement equation when receiving satellite signals normally.Second,a seven-degree-of-freedom external ballistic model is constructed,and the ideal trajectory output from the ballistic model is used to provide the virtual motion state of the projectile,which is input into a filter as a substitute observation when satellite signals are lost.Finally,an adaptive Kalman filter(AKF)is designed,the proposed adaptive Kalman filter can accurately adjust the estimation error covariance matrix and Kalman gain in real-time based on information covariance mismatch.The simulation results show that compared to the classical Kalman filter,it can reduce the average positioning error by more than 38.21%in the case of short-term and full-range loss of satellite signals,providing a new idea for the integrated navigation of projectiles with incomplete information under the condition of satellite signal loss.
文摘Single-cell RNA sequencing(scRNA-seq)is one of the most advanced sequencing technologies for studying transcriptome landscape at the single-cell revolution.It provides numerous advantages over traditional RNA-seq.Since it was first used to profile single-cell transcriptome in plants in 2019,it has been extensively employed to perform different research in plants.Recently,scRNA-seq was also quickly adopted by the cotton research community to solve lots of scientific questions which have been never solved.In this comment,we highlighted the significant progress in employing scRNA-seq to cotton genetic and genomic study and its future potential applications.
基金Supported by China National Tobacco Corporation[No.110202101048(LS-08)]Hundred’Level Innovative Talent Foundation of Guizhou Province(No.GCC[2022]028-1,GCC[2023]108)+2 种基金Guizhou Science Technology Foundation(No.ZK[2021]Key036)the National Natural Science Foundation of China(No.32160522)Guizhou Province Applied Technology Research and Development Funding Post-subsidy Project and Guizhou Tobacco Company(No.2020XM03,2020XM22,2024XM06).
文摘12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.
文摘The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiotics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the diversity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.
基金supported by the National Natural Science Foundation(81900070)the Natural Science Foundation of Hunan Province(2020JJ5813)China。
文摘The genomic fusions of the anaplastic lymphoma kinase(ALK)gene have been widely recognized as effective therapeutic targets for non-small cell lung carcinoma(NSCLC).The Second Xiangya Hospital of Central South University has treated 2 NSCLC patients with 2 distinct novel ALK gene fusions.Case 1 was a 55-year-old male with a solid nodule located in the right hilar lobe on enhanced CT scan.Case 2 was a 47-year-old female with enhanced CT showing involvement of the left upper lobe of lung.Histopathological examination of tumor tissues confirmed lung adenocarcinoma in both cases.Immunohistochemical(IHC)staining demonstrated positivity for thyroid transcription factor 1(TTF-1)and ALK-D5F3 in tumor cells,while negativity for P40.The next-generation sequencing(NGS)tests identified a PNPT1-ALK(Exon22:Exon20)fusion variant in case 1 and a TCEAL2-ALK(Exon3:Exon19)fusion variant in case 2.The TCEAL2-ALK fusion was further confirmed by amplification refractory mutation system(ARMS)-PCR at the mRNA level.Both patients were treated with oral alectinib at a dosage of 600 mg twice daily.The tumors in both patients were significantly decreased after alectinib treatment,achieving partial response.At the time of submission,there was an absence of disease progression and the progression-free survival(PFS)had surpassed 1 year.It offered compelling evidences that the individuals with NSCLC and harboring either a PNPT1-ALK(Exon22:Exon20)fusion or a TCEAL2-ALK(Exon3:Exon19)fusion,experience favorable therapeutic outcomes through the administration of alectinib.This study expands the known ALK fusion variants database and supports the precision treatment of NSCLC using ALK tyrosine kinase inhibitors(TKIs).
