摘要
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.
【目的】播种前应用有益微生物浸种是兰州百合(Lilium davidii var.unicolor)栽培中尚未应用的一项新技术。本研究利用新一代测序技术(NGS),探究这种浸种方法对真菌和细菌群落结构的影响。【方法】将百合鳞茎浸泡在一种含有有益微生物的种子处理剂(SP处理)中4小时,然后于7月种植在土壤中,并于9月取样调查植物生长状况、根际土壤理化性质和微生物群落结构。此外,还利用PICRUSt和FUNGuild软件对细菌通路和真菌功能进行了预测。【结果】在SP处理下,真菌和细菌群落结构均发生了显著变化,同时土壤养分条件也得到了改善。值得注意的是,优势微生物群落的相对丰度也发生了显著变化,如真菌Basidiomycota、Pseudeurotium、Cladophialophora、Microascus和Dactylonectria,以及细菌Proteobacteria、Chloroflexi、Ochrobactrium、Lysobacter和RB41。微生物功能预测结果表明,在SP处理下,病原真菌(包括植物病原体)数量减少,内生真菌和腐生真菌数量增加。在前20种代谢途径中,与对照CK相比,80%的代谢途径在SP处理中呈上调趋势。【结论】利用有益微生物菌剂浸种能够促进兰州百合球茎的生长。有益微生物在调节土壤微生物结构、促进内生真菌积累、减少病原体和改善土壤功能方面发挥了重要作用。此外,本研究还发现特定的微生物群参与了土壤健康的维护。
出处
《植物营养与肥料学报》
北大核心
2025年第5期994-1005,共12页
Journal of Plant Nutrition and Fertilizers
基金
国家自然科学基金项目(31860549)
甘肃省重点研发项目(22YF7NA108)
甘肃省科技重大项目(24ZDNA006)
甘肃省高校产业支撑项目(2023CYZC-49)。
作者简介
满华丽,E-mail:2502715403@qq.com;Corresponding author:杨宏羽,E-mail:171847248@qq.com;Corresponding author:师桂英,E-mail:2500544172@qq.com。
