The experimental results of Benzyl Ntertbutyloxycarbonylβcyclohexylaspartate(Ⅰ)were reported in this paper.Starting from cheap and accessible Laspartic acid,the key intermediate of βcyclohexyl Ntertbutyloxycarbonyl...The experimental results of Benzyl Ntertbutyloxycarbonylβcyclohexylaspartate(Ⅰ)were reported in this paper.Starting from cheap and accessible Laspartic acid,the key intermediate of βcyclohexyl Ntertbutyloxycarbonylaspartate(Ⅱ)was prepared by esterification of aspartic acid via cyclohexanol catalyzed by sulfuric acid and further acylation via Boc2O.The synthesis of the title compound of(Ⅰ)was performed by benzylization of(Ⅱ)through benzyl bromide.The method is facile and the yield is higher than that in literatures.The structures of these compounds were verified by1H NMR and mp.,or by the corresponding authentic compounds.The discussion of the preparative conditions of Ⅰ and Ⅱ was also given.展开更多
文摘The experimental results of Benzyl Ntertbutyloxycarbonylβcyclohexylaspartate(Ⅰ)were reported in this paper.Starting from cheap and accessible Laspartic acid,the key intermediate of βcyclohexyl Ntertbutyloxycarbonylaspartate(Ⅱ)was prepared by esterification of aspartic acid via cyclohexanol catalyzed by sulfuric acid and further acylation via Boc2O.The synthesis of the title compound of(Ⅰ)was performed by benzylization of(Ⅱ)through benzyl bromide.The method is facile and the yield is higher than that in literatures.The structures of these compounds were verified by1H NMR and mp.,or by the corresponding authentic compounds.The discussion of the preparative conditions of Ⅰ and Ⅱ was also given.
文摘3,4-二羟基扁桃酸是许多药物和香料合成的重要中间体,具有较强的抗氧化和清除自由基的活性。旨在实现3,4-二羟基扁桃酸在大肠杆菌中的从头合成。通过在大肠杆菌MG1655/ΔA中过表达来源于天蓝色链霉菌(Streptomyces coelicolor)的对羟基扁桃酸合酶(HmaS)和大肠杆菌的羟化酶(HpaBC)基因,实现了以葡萄糖为原料生物合成3,4-二羟基扁桃酸;并经IPTG和蛋白诱导温度初步优化后,摇瓶发酵36 h 3,4-二羟基扁桃酸的产量达到240 mg/L。
