摘要
采用流动注射-石英晶体微天平(FIA-QCM)和紫外光谱法研究了牛/羊血清蛋白(BSA和GSA)与扁桃酸的手性识别。在QCM表面,BSA和GSA的浓度分别为4.9×10-12和8.8×10-12mol/cm2。血清蛋白与R-扁桃酸(R-MA)作用引起QCM振动频率变化值比S-扁桃酸(S-MA)大;相应紫外结合常数也比与S-MA大,说明血清蛋白对R-MA的作用强于与S-对映体。从识别因子分析,GSA对扁桃酸的手性识别能力优于BSA。
Abstract Interactions of mandelic acid （MA） and bovine/goat serum albumins （BSA/GSA） have been investigated by FIA-QCM and UV-Vis spectroscopy, respectively. Based on frequency shift of quartz crystal, surface concentrations of immobilized BSA and GSA were 4. 9 ×10^-12 and 8.8×10^-12 mol/cm^2, respectively. After injecting mandelic acid into FIA-QCM system, frequency of the quartz crystal decreases due to interactions between enantiomers and SA molecules and the shifts between SA and R-MA are greater than those with S-enantiomer, indicating that the interactions of SA molecules with R-MA are stronger than those with S-MA. The UV binding constants for SA with S-MA are smaller than those with R-enantiomer. Taking into account the discrimination factors（ αQCM and αuv）, GSA shows a better chiral recognition with mandelic acid than BSA. Keywords serum albumin, mandelic acid, flow injection analysis-quartz crystal microbalance, UV-Vis spectroscopy, discrimination factor
出处
《应用化学》
CAS
CSCD
北大核心
2013年第9期1096-1098,共3页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(21171059)
广东省科技计划项目(2010B050300003
2010B050300021
2011B010400023)资助
关键词
血清蛋白
扁桃酸
流动注射-石英晶体微天平
紫外光谱
识别因子
serum albumin, mandelic acid, flow injection analysis-quartz crystal microbalance, UV-Vis spectroscopy, discrimination factor
作者简介
通讯联系人：章伟光，教授；Tel：020-39310210；Fax：020-39310187；E—mail：wgzhang@scnu．edu．cn；研究方向：手性分离材料的合成和色谱分析
