The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlin...The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlings of V.faba planted in this peat soil were treated with either distilled water(CK)or 25 mg·L^(−1)(G25)of functionalized graphene solution.Results showed that the height and root length of V.faba seedlings in the G25 group were significantly larger than those in CK group.The microbial com-munity was analyzed by amplifying and sequencing the 16S rRNA gene V_(3)-V_(4) region of bacteria and internal transcribed spacer re-gion of fungi in rhizosphere soil using Illumina MiSeq technology.Alpha and beta diversity analysis indicated that functionalized graphene increased the richness and diversity of bacteria and fungi in the V.faba rhizosphere peat soil.The abundances of three ni-trogen cycling-related bacteria,Hydrogenophaga,Sphingomonas and Nitrosomonadaceae,were also altered after treatment with the functionalized graphene.The relative abundance of Basilicum,related to soil phosphorus solubilization,decreased in the fungal com-munity,while the relative abundance of Clonostachys and Dimorphospora,which exhibited strong biological control over numerous fungal plant pathogens,nematodes and insects,increased in the soil after functionalized graphene treatment.Redundancy analysis re-vealed that the potential of hydrogen(pH),organic matter,and total phosphorus contributed the most to the changes in bacterial and fungal community composition in the rhizosphere soil.Overall,our findings suggested that the addition of functionalized graphene altered the relative abundances of nitrogen and phosphorus cycling-related microorganisms in peat soil,promoting changes in the physicochemical properties of the soil and ultimately leading to the improved growth of V.faba plants.展开更多
This study aimed to investigate the production of some metabolites （i.e., antibiotics, amylases and cellulases） of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil sampl...This study aimed to investigate the production of some metabolites （i.e., antibiotics, amylases and cellulases） of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil samples were collected from Camellia sinensis （L） Okuntze., Peuraria mirifca Airy Shaw Suvatabandhua., Ananus comosus Merr., Elephantopus scaber Linn., Orthosiphon grandiforus Bolding., Jatropha multifda Linn. and Senna siamea. To screen and isolate actinomycetes, the soil samples were pretreated by air-drying and subsequent heat incubation. The bacterial isolates exhibiting actinomycetes features were then randomly screened for their production of amylases, cellulases and antibiotics. It was found that 130 isolates （from 136） could produce amylases; 40 （from 107） produced cellulases; and seven （from 45） exhibited antimicrobial activity. The data of this study were preliminary, and yet demonstrated a rich diversity of rhizo-actinomycetes from medicinal plants. Besides, these organisms could be an untapped source for discovering of biotechnologically useful metabolites.展开更多
[目的]通过探究不同银杉种群根际土壤微生物群落的结构及功能差异,旨在探讨根际土壤微生物群落对银杉生长发育的影响。[方法]以广西桂林花坪国家级自然保护区的红滩、谢塘湾和野猪塘3个银杉种群为研究对象,提取其根际土壤微生物总DNA,...[目的]通过探究不同银杉种群根际土壤微生物群落的结构及功能差异,旨在探讨根际土壤微生物群落对银杉生长发育的影响。[方法]以广西桂林花坪国家级自然保护区的红滩、谢塘湾和野猪塘3个银杉种群为研究对象,提取其根际土壤微生物总DNA,进行宏基因组测序,比较分析银杉人工种群(红滩)与自然种群(谢塘湾、野猪塘)根际土壤微生物群落结构与功能之间的差异,并探索造成该差异的主导土壤环境因子。[结果](1)银杉人工种群和自然种群根际土壤微生物群落结构存在显著差异,主要表现在相对丰度占比最大的3个菌门,即变形菌门、放线菌门和酸杆菌门;(2)土壤p H值和TK含量是造成银杉人工种群和自然种群根际土壤微生物群落结构差异的主导因子;(3)银杉人工种群和自然种群根际土壤微生物群落功能存在显著差异,主要表现在甘氨酸的生物合成和代谢(Glycan biosynthesis and metabolism)、寄生虫传染病(Infectious disease:parasitic)、其他氨基酸代谢(Metabolism of other amino acids)等方面;(4)土壤p H值和TK含量是造成银杉人工种群和自然种群根际土壤微生物群落功能差异的主导因子。影响银杉根际土壤微生物群落结构与功能的土壤环境因子具有一致性。[结论]银杉的根际土壤微生物群落结构与功能在自然种群中表现得比人工种群的更复杂,但两类种群中影响其结构与功能的主导土壤环境因子是一致的,在实施银杉种苗野外回归种植时,应充分考虑根际土壤微生物群落对银杉种苗生长的影响。展开更多
文摘The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlings of V.faba planted in this peat soil were treated with either distilled water(CK)or 25 mg·L^(−1)(G25)of functionalized graphene solution.Results showed that the height and root length of V.faba seedlings in the G25 group were significantly larger than those in CK group.The microbial com-munity was analyzed by amplifying and sequencing the 16S rRNA gene V_(3)-V_(4) region of bacteria and internal transcribed spacer re-gion of fungi in rhizosphere soil using Illumina MiSeq technology.Alpha and beta diversity analysis indicated that functionalized graphene increased the richness and diversity of bacteria and fungi in the V.faba rhizosphere peat soil.The abundances of three ni-trogen cycling-related bacteria,Hydrogenophaga,Sphingomonas and Nitrosomonadaceae,were also altered after treatment with the functionalized graphene.The relative abundance of Basilicum,related to soil phosphorus solubilization,decreased in the fungal com-munity,while the relative abundance of Clonostachys and Dimorphospora,which exhibited strong biological control over numerous fungal plant pathogens,nematodes and insects,increased in the soil after functionalized graphene treatment.Redundancy analysis re-vealed that the potential of hydrogen(pH),organic matter,and total phosphorus contributed the most to the changes in bacterial and fungal community composition in the rhizosphere soil.Overall,our findings suggested that the addition of functionalized graphene altered the relative abundances of nitrogen and phosphorus cycling-related microorganisms in peat soil,promoting changes in the physicochemical properties of the soil and ultimately leading to the improved growth of V.faba plants.
文摘This study aimed to investigate the production of some metabolites （i.e., antibiotics, amylases and cellulases） of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil samples were collected from Camellia sinensis （L） Okuntze., Peuraria mirifca Airy Shaw Suvatabandhua., Ananus comosus Merr., Elephantopus scaber Linn., Orthosiphon grandiforus Bolding., Jatropha multifda Linn. and Senna siamea. To screen and isolate actinomycetes, the soil samples were pretreated by air-drying and subsequent heat incubation. The bacterial isolates exhibiting actinomycetes features were then randomly screened for their production of amylases, cellulases and antibiotics. It was found that 130 isolates （from 136） could produce amylases; 40 （from 107） produced cellulases; and seven （from 45） exhibited antimicrobial activity. The data of this study were preliminary, and yet demonstrated a rich diversity of rhizo-actinomycetes from medicinal plants. Besides, these organisms could be an untapped source for discovering of biotechnologically useful metabolites.
文摘[目的]通过探究不同银杉种群根际土壤微生物群落的结构及功能差异,旨在探讨根际土壤微生物群落对银杉生长发育的影响。[方法]以广西桂林花坪国家级自然保护区的红滩、谢塘湾和野猪塘3个银杉种群为研究对象,提取其根际土壤微生物总DNA,进行宏基因组测序,比较分析银杉人工种群(红滩)与自然种群(谢塘湾、野猪塘)根际土壤微生物群落结构与功能之间的差异,并探索造成该差异的主导土壤环境因子。[结果](1)银杉人工种群和自然种群根际土壤微生物群落结构存在显著差异,主要表现在相对丰度占比最大的3个菌门,即变形菌门、放线菌门和酸杆菌门;(2)土壤p H值和TK含量是造成银杉人工种群和自然种群根际土壤微生物群落结构差异的主导因子;(3)银杉人工种群和自然种群根际土壤微生物群落功能存在显著差异,主要表现在甘氨酸的生物合成和代谢(Glycan biosynthesis and metabolism)、寄生虫传染病(Infectious disease:parasitic)、其他氨基酸代谢(Metabolism of other amino acids)等方面;(4)土壤p H值和TK含量是造成银杉人工种群和自然种群根际土壤微生物群落功能差异的主导因子。影响银杉根际土壤微生物群落结构与功能的土壤环境因子具有一致性。[结论]银杉的根际土壤微生物群落结构与功能在自然种群中表现得比人工种群的更复杂,但两类种群中影响其结构与功能的主导土壤环境因子是一致的,在实施银杉种苗野外回归种植时,应充分考虑根际土壤微生物群落对银杉种苗生长的影响。
