To expand the study on the structures and biological activities of the anthracyclines anticancer drugs and reduce their toxic side effects,the new anthraquinone derivatives,9‑pyridylanthrahydrazone(9‑PAH)and 9,10‑bisp...To expand the study on the structures and biological activities of the anthracyclines anticancer drugs and reduce their toxic side effects,the new anthraquinone derivatives,9‑pyridylanthrahydrazone(9‑PAH)and 9,10‑bispyridylanthrahydrazone(9,10‑PAH)were designed and synthesized.Utilizing 9‑PAH and 9,10‑PAH as promising anticancer ligands,their respective copper complexes,namely[Cu(L1)Cl_(2)]Cl(1)and{[Cu_(4)(μ_(2)‑Cl)_(3)Cl_(4)(9,10‑PAH)_(2)(DMSO)_(2)]Cl_(2)}_(n)(2),were subsequently synthesized,where the new ligand L1 is formed by coupling two 9‑PAH ligands in the coordination reaction.The chemical and crystal structures of 1 and 2 were elucidated by IR,MS,elemental analysis,and single‑crystal X‑ray diffraction.Complex 1 forms a mononuclear structure.L1 coordinates with Cu through its three N atoms,together with two Cl atoms,to form a five‑coordinated square pyramidal geometry.Complex 2 constitutes a polymeric structure,wherein each structural unit centrosymmetrically encompasses two five‑coordinated binuclear copper complexes(Cu1,Cu2)of 9,10‑PAH,with similar square pyramidal geometry.A chlorine atom(Cl_(2)),located at the symmetry center,bridges Cu1 and Cu1A to connect the two binuclear copper structures.Meanwhile,the two five‑coordinated Cu2 atoms symmetrically bridge the adjacent structural units via one coordinated Cl atom,respectively,thus forming a 1D chain‑like polymeric structure.In vitro anticancer activity assessments revealed that 1 and 2 showed significant cytotoxicity even higher than cisplatin.Specifically,the IC_(50)values of 2 against HeLa‑229 and SK‑OV‑3 cancer cell lines were determined to be(5.92±0.32)μmol·L^(-1)and(6.48±0.39)μmol·L^(-1),respectively.2 could also block the proliferation of HeLa‑229 cells in S phase and significantly induce cell apoptosis.In addition,fluorescence quenching competition experiments suggested that 2 might interact with DNA by an intercalative binding mode,offering insights into its underlying anticancer mechanism.CCDC:2388918,1;2388919,2.展开更多
Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interfe...Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower cost.However,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM technology.Pain,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex differences.Despite clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are lacking.The aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG signaling.Methods This study utilized adult male and female Sprague-Dawley rats.Inflammatory pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving saline.Pain behavior was assessed using von Frey filaments at baseline and 1 h post-injection.For MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation phase.Data analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency range.Results MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA injections.Mechanical stimulation elevated alpha activity in both male and female rats pre-and post-saline/CFA injections.Saline/CFA injections augmented average power in both sexes compared to pre-injection states.Remarkably,female rats exhibited higher average spectral power 1 h after CFA injection than after saline injection during resting states.Furthermore,despite comparable pain thresholds measured by classical pain behavioral tests post-CFA treatment,female rats displayed higher average power than males in the resting state after CFA injection.Conclusion These results imply an enhanced perception of inflammatory pain in female rats compared to their male counterparts.Our study exhibits sex differences in alpha activities following CFA injection,highlighting heightened brain alpha activity in female rats during acute inflammatory pain in the resting state.Our study provides a method for OPM-based MEG recordings to be used to study brain activity in anaesthetized animals.In addition,the findings of this study contribute to a deeper understanding of pain-related neural activity and pain sex differences.展开更多
A novel 3D metal-organic framework(MOF)[Pr_(2)(L)_(3)(H_(2)O)5·H_(2)O]n(Pr-1),(H_(2)L=4,4'-oxybis(benzoic acid))with a rare structure of broken layer net,was constructed under the condition of solvothermal sy...A novel 3D metal-organic framework(MOF)[Pr_(2)(L)_(3)(H_(2)O)5·H_(2)O]n(Pr-1),(H_(2)L=4,4'-oxybis(benzoic acid))with a rare structure of broken layer net,was constructed under the condition of solvothermal synthesis.The struc-ture and crystal net were analyzed and characterized.This rod net of Pr-1 is new to both RCSR and ToposPro data-bases,and is named as rn-12 as suggested.Due to the luminescent properties of H_(2)L and Pr(Ⅲ),the solid-state fluo-rescence property and sensing performance(solvents and metal ions)of Pr-1 were investigated.The sensing experi-ments indicated that Pr-1 could act as a fluorescence sensor to detect Cd^(2+)ions with good sensitivity.In addition,antibacterial activities show that Pr-1 exhibited stronger antibacterial activity against Escherichia coli(E.coli),Staphylococcus aureus(S.aureus),and Bacillus subtilis(B.subtilis)compared to synthetic materials.展开更多
The use of carbon from waste biomass has the potential to eliminate the drawbacks of Li-S batteries and improve their overall performance.Chrome-tanned-leather-shavings(CTLS)are a readily available waste product that ...The use of carbon from waste biomass has the potential to eliminate the drawbacks of Li-S batteries and improve their overall performance.Chrome-tanned-leather-shavings(CTLS)are a readily available waste product that can be transformed into porous carbon.We prepared an ac-tivated carbon by microwave pyrolysis combined with KOH activator using the CTLS as starting materials.The carbon had a specific surface area of 556 m^(2)g^(-1) and a honeycomb-like structure.Two kinds of N-doped activated carbons were then synthesized by thermal decomposition of the activated carbon,either combined with urea,or impregnated with eth-anolamine.Both N-doped activated carbons have an in-creased number of nitrogen and amine surface groups.However,only the urea treatment was effective in improv-ing the initial capacity of the cell(1363 mAh g^(-1)),which is probably linked to the sorption of long-chain polysulfides.This investigation confirms that it is possible to use the thermal de-composition of urea to obtain carbon materials from CTLS for use as the sulfur-host cathode in Li-S batteries and improve their performance.A radial basis function neural network was fitted to provide statistical support for the experimental results,which confirmed the importance of the nitrogen content of the carbons in determining the discharge capacity of the cells.展开更多
In order to explore the remediation effects of lignite and biochar on Zn-contaminated soil,this experiment studied the impacts of adding lignite and biochar on soil respiration,soil enzyme activity,and organic carbon ...In order to explore the remediation effects of lignite and biochar on Zn-contaminated soil,this experiment studied the impacts of adding lignite and biochar on soil respiration,soil enzyme activity,and organic carbon in Zn-contaminated soil through soil culture experiments,which provided a theoretical basis for the remediation and improvement as well as for the development and utilization of Zn-contaminated soil.The study was an L8(4×2^(2))orthogonal experimental design with eight treatments,in which there were four levels of Zn contamination concentration(Z0:0;Z1:125 mg•kg^(-1);Z2:250 mg•kg^(-1);Z3:500 mg•kg^(-1)),low-Zn(125-250 mg•kg^(-1))and high-Zn(500 mg•kg^(-1)),two levels of lignite(H0:0;H1:13.33 g•kg^(-1)),two levels of biochar(C0:0;C1:3.33 g•kg^(-1)),with four replicates per treatment.The results showed that lignite or biochar and their interaction had extremely significant effects on both respiration rate and accumulation in Zn-contaminated soil.Among the high Zn-contaminated treatments,the mixed application of lignite and biochar(Z3H1C1 treatment)had the fastest soil respiration rate and the highest soil respiration accumulation.Lignite,biochar and their interaction had significant or extremely significant effects on sucrase,catalase and polyphenol oxidase activities in Zn-contaminated soil.Among the high Zn-contaminated treatments(Z3),the addition of biochar alone had the most significant effects on the increase of soil sucrase and catalase enzyme activities,while the mixed application of lignite and biochar had the most significant effects on the increase of soil polyphenol oxidase activity.Lignite,biochar and their interaction had significant or extremely significant effects on the total organic carbon,active organic carbon and microbial carbon content of Zn-contaminated soils.Soil total organic carbon content in general peaked at day 80.Among the high Zn-contaminated treatments,the addition of biochar alone had the most significant effects on the total organic carbon content of the soil,while the mixed application of lignite and biochar had the most significant effect on the microbiomass carbon content.展开更多
This study aimed to investigate the effects of dietary guanidinoacetic acid on growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.Total 48042-day-old female ducks wer...This study aimed to investigate the effects of dietary guanidinoacetic acid on growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.Total 48042-day-old female ducks were randomly divided into four groups with six replicates and 20 ducks per replicate and fed the basal diet to the control group.The experimental groups were fed the basal diet with 400,600 and 800 mg·kg^(-1) guanidinoacetic acid,respectively.The trial lasted 48 days.Compared with the control group,(1)the body weight at 90 days and average daily gain were increased(P<0.05),the feed conservation ratio was decreased(P<0.05);(2)the dressing percentage and breast muscle percentage were increased(P<0.05);(3)the total amino acid content,polyunsaturated fatty acids to monounsaturated fatty acids ratio were increased(P<0.05)in breast and thigh muscles;(4)activities of superoxide dismutase,catalase and glutathione peroxidase enzyme were increased(P<0.05)in thigh muscles;(5)the relative expressions of superoxide dismutase 1,glutathione peroxidase 1,and catalase were significantly increased(P<0.05)in the liver.In this study,the optimum dosage of 600 mg·kg^(-1) guanidinoacetic acid improved the growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.展开更多
Chemical warfare agents(CWAs)are extremely lethal substances used in warfare and terrorism,capable of causing permanent damage even in small doses,despite medical intervention.Therefore,detection,protection,and detoxi...Chemical warfare agents(CWAs)are extremely lethal substances used in warfare and terrorism,capable of causing permanent damage even in small doses,despite medical intervention.Therefore,detection,protection,and detoxification of CWAs are vital for the safety of first responders,military personnel,and civilians,driving significant research in this area.Herein,we designed and synthesized a poly(-diallyldimethylammonium chloride)(PDDA)mediated cupric oxide(CuO)functionalized activated carbon fabric(ACF),termed ACF@PDDA-CuO,as an adsorbent filter material for self-detoxifying chemical protective clothing.PDDA,a positively charged polyelectrolyte,effectively binds in-situ synthesized CuO to the negatively charged ACF surface,serving as a suitable binder.This study demonstrates the synergistic effects of PDDA-CuO functionalization on ACF,where PDDA treatment enhanced mechanical and comfort properties,and CuO crystal growth significantly improved detoxification efficacy against the CWA Nerve Agent Sarin.Comprehensive analyses,including FTIR,BET surface area analysis,SEM,EDS,TEM,STEM,TGA,XPS,and XRD,confirmed the uniform deposition of CuO and PDDA on the ACF surface.The Cu content on ACF@PDDA-CuO samples was measured via iodometric titration.The materials were evaluated for tensile strength,air permeability,water vapor permeability,nerve agent(Sarin)detoxification,and blister agent(Sulfur Mustard)breakthrough time to assess their applicability for protective clothing.The optimized PDDA-CuO on ACF detoxified 82.04%of Sarin within 18 h,compared to 25.22%by ACF alone,and enhanced tensile strength by 23.67%,air permeability by 24.63%,and water vapor permeability by 3.94%,while maintaining protection against Sulfur Mustard for 24 h.These findings indicate that ACF@PDDA-CuO is a promising candidate for CWA protective clothing,offering robust protection with enhanced comfort.展开更多
Because of an unfortunate mistake by authors,the Project(5227010679)of Foundation item was wrong.The corrected Project is shown as follows:Project(52271073).
The Taigu fault zone is one of the major 12 active boundary faults of the Shanxi fault-depression system, located on the eastern boundary of the Jinzhong basin. As the latest investigation indicated, the fault zone ha...The Taigu fault zone is one of the major 12 active boundary faults of the Shanxi fault-depression system, located on the eastern boundary of the Jinzhong basin. As the latest investigation indicated, the fault zone had dislocated gully terrace of the first order, forming fault-scarp in front of the loess mesa. It has been discovered in many places in ground surface and trenches that Holocene deposits were dislocated. The latest activity was the 1303 Hongdong earthquake M=8, the fault appeared as right-lateral strike-slip with normal faulting. During that earthquake, the Taigu fault together with the Mianshan western-side fault on the Lingshi upheaval and the Huoshan pediment fault on the eastern boundary of the Linfen basin was being active, forming a surface rupture belt of 160 km in length. Moreover, the Taigu fault were active in the mid-stage of Holocene and near 7 700 aB.P. From these we learnt that, in Shanxi fault-depression system, the run-through activity of two boundary faults of depression-basins might generate great earthquake with M=8.展开更多
Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and food plant.In the current study,the extraction of total flavonoids from S.salsa and the in vitro antioxidant,hypoglycemic...Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and food plant.In the current study,the extraction of total flavonoids from S.salsa and the in vitro antioxidant,hypoglycemic and hypolipidemic activities of the extracts were investigated.The total flavonoids was extracted by refluxing with ethanol and the selection and optimization of main experimental parameters were carried out using single factor experiments combined with central composite design-response surface methodology(CCD-RSM).The total flavonoids extracted from S.salsa were tested for hydroxyl and oxygen radical scavenging activity,and inhibitory activities againstα-amylase and lipase in vitro.Results revealed that the optimal conditions for the extraction of total flavonoids were as following:liquid-tosolid ratio,31.40:1;ethanol concentration,83.12%;reflux temperature,90℃,and reflux time,128.40 min.Under these optimized conditions,the predicted and actual values of total flavonoids yield were 0.896%and 0.932%,respectively.The in vitro antioxidant tests revealed that the total flavonoids could scavenge hydroxyl and oxygen radicals and showed inhibitory activity againstα-amylase and lipase.The optimal extraction conditions obtained in this experiment are simple to perform and have good reproducibility.The results have demonstrated that the total flavonoids from S.salsa can be used as a potential source of natural antioxidant,hypoglycemic and hypolipidemic ingredients,which provides new pharmacological basis for the development of S.salsa.展开更多
Resource-constrained project scheduling problem（RCPSP） is an important problem in research on project management. But there has been little attention paid to the objective of minimizing activities＇ cost with the re...Resource-constrained project scheduling problem（RCPSP） is an important problem in research on project management. But there has been little attention paid to the objective of minimizing activities＇ cost with the resource constraints that is a critical sub-problem in partner selection of construction supply chain management because the capacities of the renewable resources supplied by the partners will effect on the project scheduling. Its mathematic model is presented firstly, and analysis on the characteristic of the problem shows that the objective function is non-regular and the problem is NP-complete following which the basic idea for solution is clarified. Based on a definition of preposing activity cost matrix, a heuristic algorithm is brought forward. Analyses on the complexity of the heuristics and the result of numerical studies show that the heuristic algorithm is feasible and relatively effective.展开更多
The antioxidant activities and the total phenolic contents of the water and/or ethanol extracts of the nine selected culinary spices from China were systematically investigated. Both ethanol extracts and water extract...The antioxidant activities and the total phenolic contents of the water and/or ethanol extracts of the nine selected culinary spices from China were systematically investigated. Both ethanol extracts and water extracts had high ability of 1, 1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging, 2, 2＇-azinobis（3-ethylbenzthiazoline-6-sulphonic acid）（ABTS） radical scavenging and ferric reducing antioxidant power（FRAP）. The ethanol extract of Sichuan pepper showed the highest ability of DPPH radical scavenging. The extract with the highest ABTS radical scavenging effect was that of ethanol extract of cinnamon. Both ethanol and water extracts of cinnamon possessed the high ferric reducing antioxidant power（FRAP） with the values of 4 541.87 and 1 134.52 μmol of Fe（Ⅱ）/g. The extracts with high hydroxyl radical-mediated deoxyribose degradation were all the ethanol extracts as follows： cinnamon, bay leaf, Sichuan pepper, star anise and fennel. The extracts with high antioxidant activities also had the high contents of the phenols. The study indicated that these spices might be potentially be used as natural antioxidants in food.展开更多
Through the pot experiment, the effects of different cropping patterns of soybean and maize seedlings on rhizosphere soil urease, catalase, polyphenol oxidase and invertase activities and microbial biomass carbon （MB...Through the pot experiment, the effects of different cropping patterns of soybean and maize seedlings on rhizosphere soil urease, catalase, polyphenol oxidase and invertase activities and microbial biomass carbon （MBC） and nitrogen （MBN） were studied. Six treatments of soybean-soybean, soybean-maize, soybean-mixed, maize-soybean, maize-maize and maize-mixed were conducted in pots. Results showed that catalase activity and invertase activity of maize-maize were the highest and significantly different from those of the other treatments except maize-soybean; soil polyphenol oxidase activity of soybean-maize was the highest, and reached significant level among the other treatments, but there was no significant difference of urease activity among treatment soils; MBC of maize-maize soil and MBN of maize-mixed soil reached the highest and significant levels compared with other treatments; MBC and C/N ratio had positive and very significant correlations with soil catalase activity and invertase activity, respectively. Therefore, different cropping patterns could affect rhizosphere soil enzyme activities and soil MBC and MBN, which influenced soil carbon and nitrogen mineralization.展开更多
The fresh leaves and oven dried leaves from three varieties of black currant (Brodtrop,liangye,Risager)were analysed for the total contents of flavonoids by spectrophotometric methods in terms of rutin equivalent.In ...The fresh leaves and oven dried leaves from three varieties of black currant (Brodtrop,liangye,Risager)were analysed for the total contents of flavonoids by spectrophotometric methods in terms of rutin equivalent.In addition,antioxidant activities of different addition amounts of extracts of black currant (Brodtrop)leaves in lard were measured by the Oven-Storage Test,and compared with the synthetic antioxidants.The results showed the contents of the flavonoids were very rich in the leaves of three varieties from black currant.Among them the leaves of Brodtrop had the highest contents of flavonoids followed by liangye and Risager.The total contents of flavonoids in fresh leaves reach 231 70 mg/100 g,170 01 mg/100 g and 78 07 mg/100 g respectively.In the same circumstance the oven dried leaves were 397 82 mg/100 g,275 89 mg/100 g and 169 73 mg/100 g.The extracts of the leaves from Brodtrop exhibited strong antioxidant activities on lard,which increased with the addition amount of the flavonoid extracts.The antioxidant activities decreased in the order of 0 02% quercetin >0 02% BHT(butylated hydroxy toluene)>0 40% extracts>0 20% extracts>0 10% extracts>0 05% extracts>0 02% extracts>control.展开更多
Those monotonous exercises manipulated in EFL classroom,although labeled as'communicative', often result in predictable outcomes:student boredom,frustration,and even very limited progress toward mastery of the...Those monotonous exercises manipulated in EFL classroom,although labeled as'communicative', often result in predictable outcomes:student boredom,frustration,and even very limited progress toward mastery of the foreign language.This paper will introduce some oral communicative activities that can motivate students to practice conversation skills and promote interaction.展开更多
The title compound was synthesized and characterized by IR, ^1HNMR and elemental analysis. The preliminary biological test showed that the compound protected maize against injury by imazethapyr to some extent.
In order to calculate the activity coefficients of water in aqueous solution of metal electrolytes, a simplified model predicting them in ternary or multicomponent solutions with common anions from the activity data o...In order to calculate the activity coefficients of water in aqueous solution of metal electrolytes, a simplified model predicting them in ternary or multicomponent solutions with common anions from the activity data of water of the corresponding binary systems has been developed based on an assumption that the electrolytes in the solution are treated as independent particles instead of their ion forms, and the interaction of the salt( i ) salt( j ) pair in the solution is assumed to be much weaker than that of water salt pair due to the common anions of both of salts. The model was applied to the systems such as KCl H 2O NaCl, MCl H 2O M′Cl 2 (M represents Na and K, M′ represents Ca and Ba) and KCl H 2O NaCl BaCl 2 with satisfying results. The interchange energies of the studied component pairs between water and salts have also been determined by the corresponding binary aqueous solutions.展开更多
Since standardized tests do not cover the full range of important instructional objectives, this presentation deals with the use of a variety of activities in the classroom for the assessment of class participation, a...Since standardized tests do not cover the full range of important instructional objectives, this presentation deals with the use of a variety of activities in the classroom for the assessment of class participation, and explores creativity as an alternative way to conventional methods of monitoring students’ language progress and performance.展开更多
A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of ...A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of UAE were obtained by using Box-Behnken design of response surface methodology. Chromatography was carried out using a Kromasil C18 column by gradient elution with 0.1% phosphoric acid aqueous solution for HPLC-UV. All calibration curves showed good linear correlation coefficients （R^2〉0.999 6） and recoveries （from 97.3% to 104.9%） were acceptable. 1,1-diphenyl-2-picrylhydrazyl （DPPH） method was employed to test the antioxidant activity of the extract from the samples. The proposed method was successfully applied to quantifying eight components in nine samples of M.cordata, and significant variations of alkaloid contents and antioxidant aetivity of the samples from different habitats were demonstrated. It presents a powerful proof for the selection of the best sources to extract eight kinds of alkaloids.展开更多
文摘To expand the study on the structures and biological activities of the anthracyclines anticancer drugs and reduce their toxic side effects,the new anthraquinone derivatives,9‑pyridylanthrahydrazone(9‑PAH)and 9,10‑bispyridylanthrahydrazone(9,10‑PAH)were designed and synthesized.Utilizing 9‑PAH and 9,10‑PAH as promising anticancer ligands,their respective copper complexes,namely[Cu(L1)Cl_(2)]Cl(1)and{[Cu_(4)(μ_(2)‑Cl)_(3)Cl_(4)(9,10‑PAH)_(2)(DMSO)_(2)]Cl_(2)}_(n)(2),were subsequently synthesized,where the new ligand L1 is formed by coupling two 9‑PAH ligands in the coordination reaction.The chemical and crystal structures of 1 and 2 were elucidated by IR,MS,elemental analysis,and single‑crystal X‑ray diffraction.Complex 1 forms a mononuclear structure.L1 coordinates with Cu through its three N atoms,together with two Cl atoms,to form a five‑coordinated square pyramidal geometry.Complex 2 constitutes a polymeric structure,wherein each structural unit centrosymmetrically encompasses two five‑coordinated binuclear copper complexes(Cu1,Cu2)of 9,10‑PAH,with similar square pyramidal geometry.A chlorine atom(Cl_(2)),located at the symmetry center,bridges Cu1 and Cu1A to connect the two binuclear copper structures.Meanwhile,the two five‑coordinated Cu2 atoms symmetrically bridge the adjacent structural units via one coordinated Cl atom,respectively,thus forming a 1D chain‑like polymeric structure.In vitro anticancer activity assessments revealed that 1 and 2 showed significant cytotoxicity even higher than cisplatin.Specifically,the IC_(50)values of 2 against HeLa‑229 and SK‑OV‑3 cancer cell lines were determined to be(5.92±0.32)μmol·L^(-1)and(6.48±0.39)μmol·L^(-1),respectively.2 could also block the proliferation of HeLa‑229 cells in S phase and significantly induce cell apoptosis.In addition,fluorescence quenching competition experiments suggested that 2 might interact with DNA by an intercalative binding mode,offering insights into its underlying anticancer mechanism.CCDC:2388918,1;2388919,2.
文摘Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower cost.However,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM technology.Pain,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex differences.Despite clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are lacking.The aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG signaling.Methods This study utilized adult male and female Sprague-Dawley rats.Inflammatory pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving saline.Pain behavior was assessed using von Frey filaments at baseline and 1 h post-injection.For MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation phase.Data analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency range.Results MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA injections.Mechanical stimulation elevated alpha activity in both male and female rats pre-and post-saline/CFA injections.Saline/CFA injections augmented average power in both sexes compared to pre-injection states.Remarkably,female rats exhibited higher average spectral power 1 h after CFA injection than after saline injection during resting states.Furthermore,despite comparable pain thresholds measured by classical pain behavioral tests post-CFA treatment,female rats displayed higher average power than males in the resting state after CFA injection.Conclusion These results imply an enhanced perception of inflammatory pain in female rats compared to their male counterparts.Our study exhibits sex differences in alpha activities following CFA injection,highlighting heightened brain alpha activity in female rats during acute inflammatory pain in the resting state.Our study provides a method for OPM-based MEG recordings to be used to study brain activity in anaesthetized animals.In addition,the findings of this study contribute to a deeper understanding of pain-related neural activity and pain sex differences.
文摘A novel 3D metal-organic framework(MOF)[Pr_(2)(L)_(3)(H_(2)O)5·H_(2)O]n(Pr-1),(H_(2)L=4,4'-oxybis(benzoic acid))with a rare structure of broken layer net,was constructed under the condition of solvothermal synthesis.The struc-ture and crystal net were analyzed and characterized.This rod net of Pr-1 is new to both RCSR and ToposPro data-bases,and is named as rn-12 as suggested.Due to the luminescent properties of H_(2)L and Pr(Ⅲ),the solid-state fluo-rescence property and sensing performance(solvents and metal ions)of Pr-1 were investigated.The sensing experi-ments indicated that Pr-1 could act as a fluorescence sensor to detect Cd^(2+)ions with good sensitivity.In addition,antibacterial activities show that Pr-1 exhibited stronger antibacterial activity against Escherichia coli(E.coli),Staphylococcus aureus(S.aureus),and Bacillus subtilis(B.subtilis)compared to synthetic materials.
文摘The use of carbon from waste biomass has the potential to eliminate the drawbacks of Li-S batteries and improve their overall performance.Chrome-tanned-leather-shavings(CTLS)are a readily available waste product that can be transformed into porous carbon.We prepared an ac-tivated carbon by microwave pyrolysis combined with KOH activator using the CTLS as starting materials.The carbon had a specific surface area of 556 m^(2)g^(-1) and a honeycomb-like structure.Two kinds of N-doped activated carbons were then synthesized by thermal decomposition of the activated carbon,either combined with urea,or impregnated with eth-anolamine.Both N-doped activated carbons have an in-creased number of nitrogen and amine surface groups.However,only the urea treatment was effective in improv-ing the initial capacity of the cell(1363 mAh g^(-1)),which is probably linked to the sorption of long-chain polysulfides.This investigation confirms that it is possible to use the thermal de-composition of urea to obtain carbon materials from CTLS for use as the sulfur-host cathode in Li-S batteries and improve their performance.A radial basis function neural network was fitted to provide statistical support for the experimental results,which confirmed the importance of the nitrogen content of the carbons in determining the discharge capacity of the cells.
基金Supported by the Special Fund for Agro-scientific Research in Public Interest in China(201503119-06-01)。
文摘In order to explore the remediation effects of lignite and biochar on Zn-contaminated soil,this experiment studied the impacts of adding lignite and biochar on soil respiration,soil enzyme activity,and organic carbon in Zn-contaminated soil through soil culture experiments,which provided a theoretical basis for the remediation and improvement as well as for the development and utilization of Zn-contaminated soil.The study was an L8(4×2^(2))orthogonal experimental design with eight treatments,in which there were four levels of Zn contamination concentration(Z0:0;Z1:125 mg•kg^(-1);Z2:250 mg•kg^(-1);Z3:500 mg•kg^(-1)),low-Zn(125-250 mg•kg^(-1))and high-Zn(500 mg•kg^(-1)),two levels of lignite(H0:0;H1:13.33 g•kg^(-1)),two levels of biochar(C0:0;C1:3.33 g•kg^(-1)),with four replicates per treatment.The results showed that lignite or biochar and their interaction had extremely significant effects on both respiration rate and accumulation in Zn-contaminated soil.Among the high Zn-contaminated treatments,the mixed application of lignite and biochar(Z3H1C1 treatment)had the fastest soil respiration rate and the highest soil respiration accumulation.Lignite,biochar and their interaction had significant or extremely significant effects on sucrase,catalase and polyphenol oxidase activities in Zn-contaminated soil.Among the high Zn-contaminated treatments(Z3),the addition of biochar alone had the most significant effects on the increase of soil sucrase and catalase enzyme activities,while the mixed application of lignite and biochar had the most significant effects on the increase of soil polyphenol oxidase activity.Lignite,biochar and their interaction had significant or extremely significant effects on the total organic carbon,active organic carbon and microbial carbon content of Zn-contaminated soils.Soil total organic carbon content in general peaked at day 80.Among the high Zn-contaminated treatments,the addition of biochar alone had the most significant effects on the total organic carbon content of the soil,while the mixed application of lignite and biochar had the most significant effect on the microbiomass carbon content.
基金Supported by Hainan Provincial Natural Science Foundation of China(322MS134)。
文摘This study aimed to investigate the effects of dietary guanidinoacetic acid on growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.Total 48042-day-old female ducks were randomly divided into four groups with six replicates and 20 ducks per replicate and fed the basal diet to the control group.The experimental groups were fed the basal diet with 400,600 and 800 mg·kg^(-1) guanidinoacetic acid,respectively.The trial lasted 48 days.Compared with the control group,(1)the body weight at 90 days and average daily gain were increased(P<0.05),the feed conservation ratio was decreased(P<0.05);(2)the dressing percentage and breast muscle percentage were increased(P<0.05);(3)the total amino acid content,polyunsaturated fatty acids to monounsaturated fatty acids ratio were increased(P<0.05)in breast and thigh muscles;(4)activities of superoxide dismutase,catalase and glutathione peroxidase enzyme were increased(P<0.05)in thigh muscles;(5)the relative expressions of superoxide dismutase 1,glutathione peroxidase 1,and catalase were significantly increased(P<0.05)in the liver.In this study,the optimum dosage of 600 mg·kg^(-1) guanidinoacetic acid improved the growth performance,meat quality,antioxidant activity,and antioxidant-related gene expression in ducks.
基金Defence Research and Development Establishment(DRDE),DRDO,Gwalior-474002,(India)for his keen interestencouragement.The DRDE accession number for this manuscript is DRDE-IREC-130-28/03/2024.
文摘Chemical warfare agents(CWAs)are extremely lethal substances used in warfare and terrorism,capable of causing permanent damage even in small doses,despite medical intervention.Therefore,detection,protection,and detoxification of CWAs are vital for the safety of first responders,military personnel,and civilians,driving significant research in this area.Herein,we designed and synthesized a poly(-diallyldimethylammonium chloride)(PDDA)mediated cupric oxide(CuO)functionalized activated carbon fabric(ACF),termed ACF@PDDA-CuO,as an adsorbent filter material for self-detoxifying chemical protective clothing.PDDA,a positively charged polyelectrolyte,effectively binds in-situ synthesized CuO to the negatively charged ACF surface,serving as a suitable binder.This study demonstrates the synergistic effects of PDDA-CuO functionalization on ACF,where PDDA treatment enhanced mechanical and comfort properties,and CuO crystal growth significantly improved detoxification efficacy against the CWA Nerve Agent Sarin.Comprehensive analyses,including FTIR,BET surface area analysis,SEM,EDS,TEM,STEM,TGA,XPS,and XRD,confirmed the uniform deposition of CuO and PDDA on the ACF surface.The Cu content on ACF@PDDA-CuO samples was measured via iodometric titration.The materials were evaluated for tensile strength,air permeability,water vapor permeability,nerve agent(Sarin)detoxification,and blister agent(Sulfur Mustard)breakthrough time to assess their applicability for protective clothing.The optimized PDDA-CuO on ACF detoxified 82.04%of Sarin within 18 h,compared to 25.22%by ACF alone,and enhanced tensile strength by 23.67%,air permeability by 24.63%,and water vapor permeability by 3.94%,while maintaining protection against Sulfur Mustard for 24 h.These findings indicate that ACF@PDDA-CuO is a promising candidate for CWA protective clothing,offering robust protection with enhanced comfort.
文摘Because of an unfortunate mistake by authors,the Project(5227010679)of Foundation item was wrong.The corrected Project is shown as follows:Project(52271073).
基金Chinese Joint Seismological Science Foundation (201017).
文摘The Taigu fault zone is one of the major 12 active boundary faults of the Shanxi fault-depression system, located on the eastern boundary of the Jinzhong basin. As the latest investigation indicated, the fault zone had dislocated gully terrace of the first order, forming fault-scarp in front of the loess mesa. It has been discovered in many places in ground surface and trenches that Holocene deposits were dislocated. The latest activity was the 1303 Hongdong earthquake M=8, the fault appeared as right-lateral strike-slip with normal faulting. During that earthquake, the Taigu fault together with the Mianshan western-side fault on the Lingshi upheaval and the Huoshan pediment fault on the eastern boundary of the Linfen basin was being active, forming a surface rupture belt of 160 km in length. Moreover, the Taigu fault were active in the mid-stage of Holocene and near 7 700 aB.P. From these we learnt that, in Shanxi fault-depression system, the run-through activity of two boundary faults of depression-basins might generate great earthquake with M=8.
文摘Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and food plant.In the current study,the extraction of total flavonoids from S.salsa and the in vitro antioxidant,hypoglycemic and hypolipidemic activities of the extracts were investigated.The total flavonoids was extracted by refluxing with ethanol and the selection and optimization of main experimental parameters were carried out using single factor experiments combined with central composite design-response surface methodology(CCD-RSM).The total flavonoids extracted from S.salsa were tested for hydroxyl and oxygen radical scavenging activity,and inhibitory activities againstα-amylase and lipase in vitro.Results revealed that the optimal conditions for the extraction of total flavonoids were as following:liquid-tosolid ratio,31.40:1;ethanol concentration,83.12%;reflux temperature,90℃,and reflux time,128.40 min.Under these optimized conditions,the predicted and actual values of total flavonoids yield were 0.896%and 0.932%,respectively.The in vitro antioxidant tests revealed that the total flavonoids could scavenge hydroxyl and oxygen radicals and showed inhibitory activity againstα-amylase and lipase.The optimal extraction conditions obtained in this experiment are simple to perform and have good reproducibility.The results have demonstrated that the total flavonoids from S.salsa can be used as a potential source of natural antioxidant,hypoglycemic and hypolipidemic ingredients,which provides new pharmacological basis for the development of S.salsa.
文摘Resource-constrained project scheduling problem（RCPSP） is an important problem in research on project management. But there has been little attention paid to the objective of minimizing activities＇ cost with the resource constraints that is a critical sub-problem in partner selection of construction supply chain management because the capacities of the renewable resources supplied by the partners will effect on the project scheduling. Its mathematic model is presented firstly, and analysis on the characteristic of the problem shows that the objective function is non-regular and the problem is NP-complete following which the basic idea for solution is clarified. Based on a definition of preposing activity cost matrix, a heuristic algorithm is brought forward. Analyses on the complexity of the heuristics and the result of numerical studies show that the heuristic algorithm is feasible and relatively effective.
基金Supported by the National Natural Science Foundation(31201356)Agricultural Science and Technology Innovation in Jiangsu Province(cx(13)3087)the Natural Science Foundation in Jiangsu Province(BK 20130701)
文摘The antioxidant activities and the total phenolic contents of the water and/or ethanol extracts of the nine selected culinary spices from China were systematically investigated. Both ethanol extracts and water extracts had high ability of 1, 1-diphenyl-2-picrylhydrazyl（DPPH） radical scavenging, 2, 2＇-azinobis（3-ethylbenzthiazoline-6-sulphonic acid）（ABTS） radical scavenging and ferric reducing antioxidant power（FRAP）. The ethanol extract of Sichuan pepper showed the highest ability of DPPH radical scavenging. The extract with the highest ABTS radical scavenging effect was that of ethanol extract of cinnamon. Both ethanol and water extracts of cinnamon possessed the high ferric reducing antioxidant power（FRAP） with the values of 4 541.87 and 1 134.52 μmol of Fe（Ⅱ）/g. The extracts with high hydroxyl radical-mediated deoxyribose degradation were all the ethanol extracts as follows： cinnamon, bay leaf, Sichuan pepper, star anise and fennel. The extracts with high antioxidant activities also had the high contents of the phenols. The study indicated that these spices might be potentially be used as natural antioxidants in food.
基金Supported by the Scientific Research Foundation for Doctor of Northeast Agricultural UniversityInnovative Team Fund of Northeast Agricultural University
文摘Through the pot experiment, the effects of different cropping patterns of soybean and maize seedlings on rhizosphere soil urease, catalase, polyphenol oxidase and invertase activities and microbial biomass carbon （MBC） and nitrogen （MBN） were studied. Six treatments of soybean-soybean, soybean-maize, soybean-mixed, maize-soybean, maize-maize and maize-mixed were conducted in pots. Results showed that catalase activity and invertase activity of maize-maize were the highest and significantly different from those of the other treatments except maize-soybean; soil polyphenol oxidase activity of soybean-maize was the highest, and reached significant level among the other treatments, but there was no significant difference of urease activity among treatment soils; MBC of maize-maize soil and MBN of maize-mixed soil reached the highest and significant levels compared with other treatments; MBC and C/N ratio had positive and very significant correlations with soil catalase activity and invertase activity, respectively. Therefore, different cropping patterns could affect rhizosphere soil enzyme activities and soil MBC and MBN, which influenced soil carbon and nitrogen mineralization.
文摘The fresh leaves and oven dried leaves from three varieties of black currant (Brodtrop,liangye,Risager)were analysed for the total contents of flavonoids by spectrophotometric methods in terms of rutin equivalent.In addition,antioxidant activities of different addition amounts of extracts of black currant (Brodtrop)leaves in lard were measured by the Oven-Storage Test,and compared with the synthetic antioxidants.The results showed the contents of the flavonoids were very rich in the leaves of three varieties from black currant.Among them the leaves of Brodtrop had the highest contents of flavonoids followed by liangye and Risager.The total contents of flavonoids in fresh leaves reach 231 70 mg/100 g,170 01 mg/100 g and 78 07 mg/100 g respectively.In the same circumstance the oven dried leaves were 397 82 mg/100 g,275 89 mg/100 g and 169 73 mg/100 g.The extracts of the leaves from Brodtrop exhibited strong antioxidant activities on lard,which increased with the addition amount of the flavonoid extracts.The antioxidant activities decreased in the order of 0 02% quercetin >0 02% BHT(butylated hydroxy toluene)>0 40% extracts>0 20% extracts>0 10% extracts>0 05% extracts>0 02% extracts>control.
文摘Those monotonous exercises manipulated in EFL classroom,although labeled as'communicative', often result in predictable outcomes:student boredom,frustration,and even very limited progress toward mastery of the foreign language.This paper will introduce some oral communicative activities that can motivate students to practice conversation skills and promote interaction.
基金Supported by National Basic Research Program of China (30171069)The Natural Science Foundation of Heilongjiang Province (B200602)The Re-search Science Foundation in Technology Innovation of Harbin (2006RFXXN005)
文摘The title compound was synthesized and characterized by IR, ^1HNMR and elemental analysis. The preliminary biological test showed that the compound protected maize against injury by imazethapyr to some extent.
文摘In order to calculate the activity coefficients of water in aqueous solution of metal electrolytes, a simplified model predicting them in ternary or multicomponent solutions with common anions from the activity data of water of the corresponding binary systems has been developed based on an assumption that the electrolytes in the solution are treated as independent particles instead of their ion forms, and the interaction of the salt( i ) salt( j ) pair in the solution is assumed to be much weaker than that of water salt pair due to the common anions of both of salts. The model was applied to the systems such as KCl H 2O NaCl, MCl H 2O M′Cl 2 (M represents Na and K, M′ represents Ca and Ba) and KCl H 2O NaCl BaCl 2 with satisfying results. The interchange energies of the studied component pairs between water and salts have also been determined by the corresponding binary aqueous solutions.
文摘Since standardized tests do not cover the full range of important instructional objectives, this presentation deals with the use of a variety of activities in the classroom for the assessment of class participation, and explores creativity as an alternative way to conventional methods of monitoring students’ language progress and performance.
基金Project(20576142) supposed by the National Natural Science Foundation of China Project(2009DFA31270) supported by the International Cooperation Project of Ministry of Science and Technology of China
文摘A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of UAE were obtained by using Box-Behnken design of response surface methodology. Chromatography was carried out using a Kromasil C18 column by gradient elution with 0.1% phosphoric acid aqueous solution for HPLC-UV. All calibration curves showed good linear correlation coefficients （R^2〉0.999 6） and recoveries （from 97.3% to 104.9%） were acceptable. 1,1-diphenyl-2-picrylhydrazyl （DPPH） method was employed to test the antioxidant activity of the extract from the samples. The proposed method was successfully applied to quantifying eight components in nine samples of M.cordata, and significant variations of alkaloid contents and antioxidant aetivity of the samples from different habitats were demonstrated. It presents a powerful proof for the selection of the best sources to extract eight kinds of alkaloids.
