The possibility of identifying gunshot residue (GSR) particles produced by non-toxic primers containing only titanium and zinc is a very difficult task using SEM/EDX analysis employed in the analysis of GSR originatin...The possibility of identifying gunshot residue (GSR) particles produced by non-toxic primers containing only titanium and zinc is a very difficult task using SEM/EDX analysis employed in the analysis of GSR originating from primers containing lead, barium and antimony. However, Bauer et al. demonstrated that non-toxic (TieZn) primers form a TiZn2O4 spinel crystalline structure using SEM/EDX with EBSD (Electron Back Scatter Diffraction) and TKD (Transmission Kikuchi Diffraction), whereas GSR originating from gadolinium-doped TieZn primers form a non-crystalline glass phase. Here, a possible explanation of these different phenomena is hypothesized.展开更多
The availability of the plastid genome sequences is one of the bases for comparative,functional,and structural genomic studies of plastid-containing living organisms,in addition to the application
[目的]设计能用于多重RT-PCR扩增禽流感病毒H5、H7亚型基因的引物。[方法]在GenBank中搜索H5、H7亚型禽流感病毒的基因序列,并利用DNAStar分析其同源性,采用Primer Premier 5.0软件设计引物。[结果]多重RT-PCR扩增、琼脂糖凝胶电泳及测...[目的]设计能用于多重RT-PCR扩增禽流感病毒H5、H7亚型基因的引物。[方法]在GenBank中搜索H5、H7亚型禽流感病毒的基因序列,并利用DNAStar分析其同源性,采用Primer Premier 5.0软件设计引物。[结果]多重RT-PCR扩增、琼脂糖凝胶电泳及测序结果显示引物设计成功。[结论]该方法用于禽流感病毒的快速诊断是可行的。展开更多
Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polym...Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polymerasessynthesize DNA in the 5’ to 3’ direction and require aRNA primer for initiation, telomeric DNA could belost at chromosome ends unless the termini arespecifically extended by telomerase. Telomerase is aspecialized ribonucleoprotein polymerase that containsan integral RNA with a short template element展开更多
Glandless or gossypol free cottons are of greatvalue to develop)the utilizations of cottonseedsand cotton scientists have been interested indeveloping more applicable glandless cultivars.Common glandless cotton are ge...Glandless or gossypol free cottons are of greatvalue to develop)the utilizations of cottonseedsand cotton scientists have been interested indeveloping more applicable glandless cultivars.Common glandless cotton are generated byrecessive genes,such as gl<sub>2</sub> and gl<sub>3</sub>.A dominantglandless mutant has been discovered展开更多
RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.Th...RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The展开更多
文摘The possibility of identifying gunshot residue (GSR) particles produced by non-toxic primers containing only titanium and zinc is a very difficult task using SEM/EDX analysis employed in the analysis of GSR originating from primers containing lead, barium and antimony. However, Bauer et al. demonstrated that non-toxic (TieZn) primers form a TiZn2O4 spinel crystalline structure using SEM/EDX with EBSD (Electron Back Scatter Diffraction) and TKD (Transmission Kikuchi Diffraction), whereas GSR originating from gadolinium-doped TieZn primers form a non-crystalline glass phase. Here, a possible explanation of these different phenomena is hypothesized.
文摘The availability of the plastid genome sequences is one of the bases for comparative,functional,and structural genomic studies of plastid-containing living organisms,in addition to the application
文摘[目的]设计能用于多重RT-PCR扩增禽流感病毒H5、H7亚型基因的引物。[方法]在GenBank中搜索H5、H7亚型禽流感病毒的基因序列,并利用DNAStar分析其同源性,采用Primer Premier 5.0软件设计引物。[结果]多重RT-PCR扩增、琼脂糖凝胶电泳及测序结果显示引物设计成功。[结论]该方法用于禽流感病毒的快速诊断是可行的。
文摘Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polymerasessynthesize DNA in the 5’ to 3’ direction and require aRNA primer for initiation, telomeric DNA could belost at chromosome ends unless the termini arespecifically extended by telomerase. Telomerase is aspecialized ribonucleoprotein polymerase that containsan integral RNA with a short template element
文摘Glandless or gossypol free cottons are of greatvalue to develop)the utilizations of cottonseedsand cotton scientists have been interested indeveloping more applicable glandless cultivars.Common glandless cotton are generated byrecessive genes,such as gl<sub>2</sub> and gl<sub>3</sub>.A dominantglandless mutant has been discovered
文摘RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The
