Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16...Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16S rDNA sequence analysis. Five virulence factors were detected, then artificial infection experiment and histopathological method were carried out. These results showed that strain YER6022 was one of Y. enterocolitica family members. In addition, ail, ystb, virF, yadA and HPIint were dectected. In artificial infection experiment, with 80% mortality and 100% morbidity, injected Pelteobagrus vachellis showed red swollen of the anus, abdomen swelling and fim bleeding. There existed serious hyperaemia and edema in kidney, spleen, intestine and liver at the light microscope. Ultrastructural observation indicated that mitochondria of the liver, kidney, spleen and intestine swelled and mitochondrial cristae broke. The data had further shed light on its pathogenicity in Pelteobagrus vachelli. It would benefit for further studies on pathogenesis ofPelteobagrus vachelli infected with Y. enterocolitica.展开更多
Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish （Pelteobagrus fulvidraco） were irradiated by UV suspending in the synthetic ovari...Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish （Pelteobagrus fulvidraco） were irradiated by UV suspending in the synthetic ovarian fluid （OF）, and the total dosage of UV irradiation was 220 mJ·cm^-2. Diploid could be induced by heat shock （40℃, 2 min, 3 min） with different time period after fertilization （15-37 min）. The result showed that heat shock with 2 min was better than 3 min; there were two apices of induction in 17-21 min and 27-31 min after fertilization. The highest hatching rate was 3.30% at 29 min after fertilization, and the difference between two apices of induction was unobvious.展开更多
将黄颡鱼(Pelteobagrus fulvidraco)受精卵分别加入到单含不同浓度有效微生物EM(E ffective m icroorgan-ism,EM)及含不同浓度EM和50μL/L的重氢硫酸盐复合物的静水中孵化,研究EM和重氢硫酸盐对鱼卵受精率、水霉病发生率的影响。结果表...将黄颡鱼(Pelteobagrus fulvidraco)受精卵分别加入到单含不同浓度有效微生物EM(E ffective m icroorgan-ism,EM)及含不同浓度EM和50μL/L的重氢硫酸盐复合物的静水中孵化,研究EM和重氢硫酸盐对鱼卵受精率、水霉病发生率的影响。结果表明,EM和重氢硫酸盐均可有效地抑制鱼卵孵化过程中水霉病的发生,提高鱼卵的受精率;而EM及重氢硫酸盐联合作用能有效地延长抑制水霉病发生的时间。当EM与重氢硫酸盐联合使用的浓度分别为8μL/L和50μL/L时,对水霉病具有更强的抑制作用。展开更多
基金Supported by the Science&Technology Department of Sichuan Province(2013FZ0014)the Construction Project of the Postgraduate Academic Degree in Southwest University for Nationalities(2013XWD-S071007)
文摘Yersinia enterocolitica is an important zoonotic pathogen that can induce disease outbreaks in a wide host range. Strain YER6022 was isolated from Pelteobagrus vachelli and identified using bacterial morphology and 16S rDNA sequence analysis. Five virulence factors were detected, then artificial infection experiment and histopathological method were carried out. These results showed that strain YER6022 was one of Y. enterocolitica family members. In addition, ail, ystb, virF, yadA and HPIint were dectected. In artificial infection experiment, with 80% mortality and 100% morbidity, injected Pelteobagrus vachellis showed red swollen of the anus, abdomen swelling and fim bleeding. There existed serious hyperaemia and edema in kidney, spleen, intestine and liver at the light microscope. Ultrastructural observation indicated that mitochondria of the liver, kidney, spleen and intestine swelled and mitochondrial cristae broke. The data had further shed light on its pathogenicity in Pelteobagrus vachelli. It would benefit for further studies on pathogenesis ofPelteobagrus vachelli infected with Y. enterocolitica.
基金Supported by Science Project of Heilongjiang Province(GC03B510)
文摘Androgenesis was the especial zoogamy that the germ plasma of offspring was from the agnate. In this study the eggs of Yellow catfish （Pelteobagrus fulvidraco） were irradiated by UV suspending in the synthetic ovarian fluid （OF）, and the total dosage of UV irradiation was 220 mJ·cm^-2. Diploid could be induced by heat shock （40℃, 2 min, 3 min） with different time period after fertilization （15-37 min）. The result showed that heat shock with 2 min was better than 3 min; there were two apices of induction in 17-21 min and 27-31 min after fertilization. The highest hatching rate was 3.30% at 29 min after fertilization, and the difference between two apices of induction was unobvious.
文摘将黄颡鱼(Pelteobagrus fulvidraco)受精卵分别加入到单含不同浓度有效微生物EM(E ffective m icroorgan-ism,EM)及含不同浓度EM和50μL/L的重氢硫酸盐复合物的静水中孵化,研究EM和重氢硫酸盐对鱼卵受精率、水霉病发生率的影响。结果表明,EM和重氢硫酸盐均可有效地抑制鱼卵孵化过程中水霉病的发生,提高鱼卵的受精率;而EM及重氢硫酸盐联合作用能有效地延长抑制水霉病发生的时间。当EM与重氢硫酸盐联合使用的浓度分别为8μL/L和50μL/L时,对水霉病具有更强的抑制作用。
