In order to solve the problems which are widespread in potato seeding planter, such as lower operating efficiency, unideal performance index, higher replay rate and leakage rate and uneven spacing sowing in seeding op...In order to solve the problems which are widespread in potato seeding planter, such as lower operating efficiency, unideal performance index, higher replay rate and leakage rate and uneven spacing sowing in seeding operating, a trailed potato fertilization seeder was designed. The key components of the special structure had been got through the description of the structure and working principle of the whole machine. A crossing seed-taken technology along with the vibration component was adopted to achieve precision seeding. The results showed in the feld test of the machine： all the performance indexes of the machine met the agronomic requirement of potato planting; the multiple and missing index were low; it completed the ditching, fertilizing, seeding as well as ridging in one planting process; its operation effciency was high and the stability was good. This design of the trailed potato fertilization seeder provided a reference for the development of large traction-type potato seeding machine.展开更多
Based on the sweet potato starch,cationic starch,acetic starch and cationic-acetic compoundedly modified starch were made through chemical denaturalization.The above three kinds of static rheological parameter and dyn...Based on the sweet potato starch,cationic starch,acetic starch and cationic-acetic compoundedly modified starch were made through chemical denaturalization.The above three kinds of static rheological parameter and dynamic rheological parameter were measured,respectively.The experimental result reveals that the thermal stability of starchy viscosity increases after chemical denaturalization.Under the condition of identical shearing rate,the shear stress of cationic-acetic ester compoundedly modified sweet potato starch paste is the largest among these kinds of sweet potato starch.This attributes to a phenomenon of shearing thinning.Furthermore,raw sweet potato starch has a larger gel intensity than that of modified starch.展开更多
DNA fingerprints of 37 PVY resistant potato varieties （clones） were generated using PCR-based RAPD analysis. All 37 varieties were differentiated by banding patterns obtained from 17 primers that generated 164 polym...DNA fingerprints of 37 PVY resistant potato varieties （clones） were generated using PCR-based RAPD analysis. All 37 varieties were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0.27 and 0.50, with average value being 0.35. The average link clustering algorithm was used to construct a dendrogram with Statistica. They were classified into three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of varieties （clones） of similar pedigree. This study has shown that RAPD marker is a useful tool for potato variety identification, differentiation, and estimation of genetic relationships.展开更多
Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. ...Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. Concentration and gradient experiments for four components （enzyme, MgCl2, DNA template and dNTPs） in PCR system were used in the research with the concentration of the other component remained the same; the orthogonal design L9 （34） was applied in the optimization of four sets of primers （STM0014, Pat, SSI, and UGP） in the reaction system at three levels; the temperature gradient selection was used to find out the optimum annealing temperature for the primer. The optimized multiplex PCR system of potato SSR marker with a total volume of 20 μL ： 2.5 μL 25 mmol.L-1 MgCl2, 0.6 μL 10 mmol·L-1 dNTPs, 0.8 U Taq, 80 ng DNA template was ultimately established through the comparison and analysis of test results; the ratio of four pairs of 4 mmol. L1 primers was 2 ： 1 ： 2 ： 3, and the annealing temperature was 54.7℃. The optimized reaction system could be repeated stably; and the stable and reliable amplification results were able to clearly distinguish different potato varieties. This research built the solid foundation for the further study of genetic diversity of potato germplasms and construction of DNA fingerprinting..展开更多
Potato is one of the important food crops in Shangnan County,Shaanxi Province.Potato planting strategy in this region has great significance for potato planting,according to local conditions and for optimizing regiona...Potato is one of the important food crops in Shangnan County,Shaanxi Province.Potato planting strategy in this region has great significance for potato planting,according to local conditions and for optimizing regional layout.The ecological suitability of potato cultivation in Shangnan County was evaluated by using geographic information system(GIS)technology,county cultivated land resource management information system and analytic hierarchy process(AHP).The evaluation index system of ecological suitability of potatoes in Shangnan County was established by screening the evaluation index of each ecological factor with expert experience method.The AHP and fuzzy evaluation method were applied to the quantitative calculation of the weight of each index.The ecological suitability assessment map of potato planting in Shangnan County was obtained by using the overlay analysis function of ArcGIS 10.2.The results showed that highly suitable area for potato cultivation was 6974.04 hm^2,accounting for 28.07%of the cultivated land.The suitable area was 11205.75 hm^2,accounting for 45.10%of the cultivated land area.These two types of arable land mainly distributed in the shallow hilly area,the middle mountain landform and the river valley area.The remaining arable land in this region mainly distributed in the shallow hills and the mid-mountain landscapes,including the arable land which was reluctantly suitable for potato cultivation and unsuitable for potato cultivation,with 5646.15 and 1022.33 hm^2,respectively,accounting for only 22.72%and 4.11%of the total arable land areas.Therefore,based on the statistics and analyses of potato planting environment in Shangnan County,the pattern of local ecological adaptability distribution was summarized and the spatial distribution map was drawn,thus providing scientific basis for rational distribution and planning of potato in Shangnan County.展开更多
Potato spindle tuber viroid(PSTVd)disease is one of the major diseases that threatens potato production.Therefore,an advanced,rapid and sensitive detection technology is needed to detect the disease for better control...Potato spindle tuber viroid(PSTVd)disease is one of the major diseases that threatens potato production.Therefore,an advanced,rapid and sensitive detection technology is needed to detect the disease for better control.In order to establish an easier nucleic acid spot hybridization(NASH)method,some studies were tried as the followings:(1)the pre-hybridization step of nucleic acid spot hybridization(NASH)was omitted compared with ordinary way;(2)RNA extraction(phenol extraction and Ames buffer extraction)methods were compared;(3)fixed RNA by UV lamp and oven compared with UV cross-linker;(4)hybridized the RNA in shaking incubator and so on.The results showed that RNA extracted by Ames buffer was more effective than by the phenol extraction method.Besides,the result of hybridization without pre-hybridization step was better than that with 1.5 h of pre-hybridization.The more important discovery was that the shaking incubator could replace the hybridization oven and the ordinary UV lamp could replace the UV cross-linker.After a long term repeated research and testing,a new hybridization system that could rapidly detect the PSTVd by improved NASH technique merely using common instruments and equipment was established.展开更多
Diploid hybrid derived from dihaploid Neo-tuberosum × Solanum phurejaand progeny of the diploid hybrid backcrossed to dihaploid Neo-tuberosum,which could produce 2n pollen, were crossed as male to S.tuberosumssp....Diploid hybrid derived from dihaploid Neo-tuberosum × Solanum phurejaand progeny of the diploid hybrid backcrossed to dihaploid Neo-tuberosum,which could produce 2n pollen, were crossed as male to S.tuberosumssp. tubersumc.v. NEA303. The performance of the 4x hybrids obtained from 4x × 2x cross was investigated for yield and yield components in the first year clonal generation. The 4x hybrids shown a drastic heterosis in plant height. Marketable yield/plant for the 4x hybrid was no better than that for the 4x cultivar, but the best hybrid individual of the two hybrid populations exceeded the best individual of the cultivar by 260 g/plant and 60 g/plant, respectively. Total yield/plant for the 4x hybrids performed drastic heterosis, however tuber number/plant was high and mean tuber weight low. These limit the use of 4x × 2x hybrids in potato breeding programs and need to be improved in near future.展开更多
Particle size, rheological and thermal properties of potato starch from Yunnan province of China was in-vestigated. The particle size ranges from 0.429-102.3 um determined by laser light-scatter. The major flow type o...Particle size, rheological and thermal properties of potato starch from Yunnan province of China was in-vestigated. The particle size ranges from 0.429-102.3 um determined by laser light-scatter. The major flow type of 6 w/v% potato starch was shear-thinning fluid even the shear rate up to 800·s-1, and the gel formed by 6 w/v% potato starch fell to weak gel for its little difference between G' and G'', high dependence on frequency and low value of G'(Pa). The hardness and cohesiveness of potato starch gel were 31.3 g and 131.9 g·s, respectively. The thermal properties of potato starch were also determined by DSC at the starch:water=3:1. The To, Tp, and ΔH of potato starch were 62.23℃,67.31℃, and 2.22 J·g-1.展开更多
Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters....Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters. The range of variation for all the characters were found to be wide and pronounced which indicated that characters were quantitative in nature and were under polygenic control. For all the characters, greater portion of the total δ2p was mostly contributed by the δ2g. Among the seven characters, tuber weight per plant showed the highest δ2g, PCV, GCV, H2b, GA and expected GA%. On the other hand, dry matter contents (%) showed the lowest H2b. Phenotypic and genotypic correlation coefficients of plant height with no. of tubers per plant and tuber weight per plant; no. of branches per plant with no. of tubers per plant and tuber weight per plant were found to be significant and positive.展开更多
Ten crosses involving seven parents crossed in line ×tester design were studied for main stem height.plant-1,main stem leaves.plant-1,stems.plant-1,plant weight.plant-1,average tuber weight.plant-1 and tuber dry ...Ten crosses involving seven parents crossed in line ×tester design were studied for main stem height.plant-1,main stem leaves.plant-1,stems.plant-1,plant weight.plant-1,average tuber weight.plant-1 and tuber dry matter %in F1 generation.The additive gene action was found to be more important for the characters,main stem height.plant-1,main stem leaves.plant-1,stems.plant-1,plant weight.plant-1,and average tuber weight.plant-1.Among seven parents,Lal-silbilati,Lalpakri and Ausha were good combiners for the characters,main stem height.plant-1,main stem leaves.plant-1andaverage tuber weight.plant-1,respectively.For average tuber weight.plant-1,the crosses Patnae/TPS-67 and Lalpakri/TPS-13 showed significant positive specific combining ability effects.Ausha/TPS-67 showed significant positive specific com-bining ability effects for main stem leaves.plant-1.展开更多
A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promoters and the presence or absence of the β-glucuronidase(gus) reporter gene.These constructs were i...A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promoters and the presence or absence of the β-glucuronidase(gus) reporter gene.These constructs were integrated into potato cv.Spunta by Agrobacterium tumefaciens-mediated transformation.Highest expression of cryV gene,determined by mRNA levels and insect mortality,was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%,respectively,for potato tuber moth(Phthorimaea operculella Zeller) in the transgenic lines.Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene.Bt expression was also facilitated by using the(ocs)3 mas super promoter,whereas the Bt expression regulated by the patatin promoter(tuber-specific) was too low to have any effect upon the mortality of potato tuber moth.These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes.展开更多
为研究不同比例明胶/马铃薯淀粉对大豆分离蛋白(soybean protein isolate,SPI)凝胶性能及3D打印性能的影响,该文以SPI、明胶和马铃薯淀粉为原料,通过热诱导的方式制备SPI复合凝胶,并对复合凝胶的水分分布、质构、流变特性、结构、微观...为研究不同比例明胶/马铃薯淀粉对大豆分离蛋白(soybean protein isolate,SPI)凝胶性能及3D打印性能的影响,该文以SPI、明胶和马铃薯淀粉为原料,通过热诱导的方式制备SPI复合凝胶,并对复合凝胶的水分分布、质构、流变特性、结构、微观结构以及3D打印特性等进行了表征。结果表明,明胶与马铃薯淀粉比例在1∶8~6∶8(质量比,下同)范围内,随着比例增加,自由水比例降低,不易流动水比例升高,明胶与马铃薯淀粉比例为6∶8时,不易流动水比例最高,占比为2.48%。复配凝胶的硬度、内聚性和咀嚼性随着明胶与马铃薯淀粉比例的增加逐渐降低,6∶8时达到最低,质地最为柔软。随着剪切速率升高,凝胶的黏度均下降,样品的G′和G″随着明胶与马铃薯淀粉比例的增加也均呈升高趋势。傅里叶红外光谱结果显示,添加了明胶和马铃薯淀粉的样品在波长3295.44 cm-1处的—OH振动发生红移,氢键作用增强。随着明胶与马铃薯淀粉比例的增加,复配凝胶孔径逐渐减小,明胶与马铃薯淀粉比例为6∶8时,凝胶孔隙分布呈现出最高的均匀性,打印精度最好,高度打印精确度99.60%,直径打印精确度99.92%,且在1 h内未出现明显塌陷。综上所述,明胶/马铃薯淀粉比例为6∶8时,对大豆分离蛋白凝胶3D打印性能的改善最为显著,这为开发蛋白基3D打印油墨提供理论依据。展开更多
基金Supported by Funding(2016YFD0701600)from Research and Development of Potato Harvesting Technology and EquipmentSpecial Funds for Construction of Modern Agricultural Technology System(CARS-10-P22)Study on Key Technical Equipment for Scale of Potato Planting(GA15B401)
文摘In order to solve the problems which are widespread in potato seeding planter, such as lower operating efficiency, unideal performance index, higher replay rate and leakage rate and uneven spacing sowing in seeding operating, a trailed potato fertilization seeder was designed. The key components of the special structure had been got through the description of the structure and working principle of the whole machine. A crossing seed-taken technology along with the vibration component was adopted to achieve precision seeding. The results showed in the feld test of the machine： all the performance indexes of the machine met the agronomic requirement of potato planting; the multiple and missing index were low; it completed the ditching, fertilizing, seeding as well as ridging in one planting process; its operation effciency was high and the stability was good. This design of the trailed potato fertilization seeder provided a reference for the development of large traction-type potato seeding machine.
基金Project(2007FJ1007) supported by the Science Foundation of Hunan Province,China
文摘Based on the sweet potato starch,cationic starch,acetic starch and cationic-acetic compoundedly modified starch were made through chemical denaturalization.The above three kinds of static rheological parameter and dynamic rheological parameter were measured,respectively.The experimental result reveals that the thermal stability of starchy viscosity increases after chemical denaturalization.Under the condition of identical shearing rate,the shear stress of cationic-acetic ester compoundedly modified sweet potato starch paste is the largest among these kinds of sweet potato starch.This attributes to a phenomenon of shearing thinning.Furthermore,raw sweet potato starch has a larger gel intensity than that of modified starch.
文摘DNA fingerprints of 37 PVY resistant potato varieties （clones） were generated using PCR-based RAPD analysis. All 37 varieties were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0.27 and 0.50, with average value being 0.35. The average link clustering algorithm was used to construct a dendrogram with Statistica. They were classified into three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of varieties （clones） of similar pedigree. This study has shown that RAPD marker is a useful tool for potato variety identification, differentiation, and estimation of genetic relationships.
基金Supported by the International Cooperation Project of Heilongjiang Science and Technology Department (WC05B08)
文摘Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. Concentration and gradient experiments for four components （enzyme, MgCl2, DNA template and dNTPs） in PCR system were used in the research with the concentration of the other component remained the same; the orthogonal design L9 （34） was applied in the optimization of four sets of primers （STM0014, Pat, SSI, and UGP） in the reaction system at three levels; the temperature gradient selection was used to find out the optimum annealing temperature for the primer. The optimized multiplex PCR system of potato SSR marker with a total volume of 20 μL ： 2.5 μL 25 mmol.L-1 MgCl2, 0.6 μL 10 mmol·L-1 dNTPs, 0.8 U Taq, 80 ng DNA template was ultimately established through the comparison and analysis of test results; the ratio of four pairs of 4 mmol. L1 primers was 2 ： 1 ： 2 ： 3, and the annealing temperature was 54.7℃. The optimized reaction system could be repeated stably; and the stable and reliable amplification results were able to clearly distinguish different potato varieties. This research built the solid foundation for the further study of genetic diversity of potato germplasms and construction of DNA fingerprinting..
基金Supported by the National 863 Program Project"Express Acquisition and Analysis Technology of Crop Growth Information"(2013AA102401)the National"Twelfth Five-year Plan"Science and Technology Support Plan"Accurate Acquisition Technology Integration and Application of Farmland Information on Multi-remote Sensing Platform in Dry Areas"(2012 BAH29B04)Assessment Project of Cultivated Land Fertility in Shaanxi Province。
文摘Potato is one of the important food crops in Shangnan County,Shaanxi Province.Potato planting strategy in this region has great significance for potato planting,according to local conditions and for optimizing regional layout.The ecological suitability of potato cultivation in Shangnan County was evaluated by using geographic information system(GIS)technology,county cultivated land resource management information system and analytic hierarchy process(AHP).The evaluation index system of ecological suitability of potatoes in Shangnan County was established by screening the evaluation index of each ecological factor with expert experience method.The AHP and fuzzy evaluation method were applied to the quantitative calculation of the weight of each index.The ecological suitability assessment map of potato planting in Shangnan County was obtained by using the overlay analysis function of ArcGIS 10.2.The results showed that highly suitable area for potato cultivation was 6974.04 hm^2,accounting for 28.07%of the cultivated land.The suitable area was 11205.75 hm^2,accounting for 45.10%of the cultivated land area.These two types of arable land mainly distributed in the shallow hilly area,the middle mountain landform and the river valley area.The remaining arable land in this region mainly distributed in the shallow hills and the mid-mountain landscapes,including the arable land which was reluctantly suitable for potato cultivation and unsuitable for potato cultivation,with 5646.15 and 1022.33 hm^2,respectively,accounting for only 22.72%and 4.11%of the total arable land areas.Therefore,based on the statistics and analyses of potato planting environment in Shangnan County,the pattern of local ecological adaptability distribution was summarized and the spatial distribution map was drawn,thus providing scientific basis for rational distribution and planning of potato in Shangnan County.
基金Supported by Doctoral Research Startup Project of Zhaotong University。
文摘Potato spindle tuber viroid(PSTVd)disease is one of the major diseases that threatens potato production.Therefore,an advanced,rapid and sensitive detection technology is needed to detect the disease for better control.In order to establish an easier nucleic acid spot hybridization(NASH)method,some studies were tried as the followings:(1)the pre-hybridization step of nucleic acid spot hybridization(NASH)was omitted compared with ordinary way;(2)RNA extraction(phenol extraction and Ames buffer extraction)methods were compared;(3)fixed RNA by UV lamp and oven compared with UV cross-linker;(4)hybridized the RNA in shaking incubator and so on.The results showed that RNA extracted by Ames buffer was more effective than by the phenol extraction method.Besides,the result of hybridization without pre-hybridization step was better than that with 1.5 h of pre-hybridization.The more important discovery was that the shaking incubator could replace the hybridization oven and the ordinary UV lamp could replace the UV cross-linker.After a long term repeated research and testing,a new hybridization system that could rapidly detect the PSTVd by improved NASH technique merely using common instruments and equipment was established.
文摘Diploid hybrid derived from dihaploid Neo-tuberosum × Solanum phurejaand progeny of the diploid hybrid backcrossed to dihaploid Neo-tuberosum,which could produce 2n pollen, were crossed as male to S.tuberosumssp. tubersumc.v. NEA303. The performance of the 4x hybrids obtained from 4x × 2x cross was investigated for yield and yield components in the first year clonal generation. The 4x hybrids shown a drastic heterosis in plant height. Marketable yield/plant for the 4x hybrid was no better than that for the 4x cultivar, but the best hybrid individual of the two hybrid populations exceeded the best individual of the cultivar by 260 g/plant and 60 g/plant, respectively. Total yield/plant for the 4x hybrids performed drastic heterosis, however tuber number/plant was high and mean tuber weight low. These limit the use of 4x × 2x hybrids in potato breeding programs and need to be improved in near future.
文摘Particle size, rheological and thermal properties of potato starch from Yunnan province of China was in-vestigated. The particle size ranges from 0.429-102.3 um determined by laser light-scatter. The major flow type of 6 w/v% potato starch was shear-thinning fluid even the shear rate up to 800·s-1, and the gel formed by 6 w/v% potato starch fell to weak gel for its little difference between G' and G'', high dependence on frequency and low value of G'(Pa). The hardness and cohesiveness of potato starch gel were 31.3 g and 131.9 g·s, respectively. The thermal properties of potato starch were also determined by DSC at the starch:water=3:1. The To, Tp, and ΔH of potato starch were 62.23℃,67.31℃, and 2.22 J·g-1.
文摘Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters. The range of variation for all the characters were found to be wide and pronounced which indicated that characters were quantitative in nature and were under polygenic control. For all the characters, greater portion of the total δ2p was mostly contributed by the δ2g. Among the seven characters, tuber weight per plant showed the highest δ2g, PCV, GCV, H2b, GA and expected GA%. On the other hand, dry matter contents (%) showed the lowest H2b. Phenotypic and genotypic correlation coefficients of plant height with no. of tubers per plant and tuber weight per plant; no. of branches per plant with no. of tubers per plant and tuber weight per plant were found to be significant and positive.
文摘Ten crosses involving seven parents crossed in line ×tester design were studied for main stem height.plant-1,main stem leaves.plant-1,stems.plant-1,plant weight.plant-1,average tuber weight.plant-1 and tuber dry matter %in F1 generation.The additive gene action was found to be more important for the characters,main stem height.plant-1,main stem leaves.plant-1,stems.plant-1,plant weight.plant-1,and average tuber weight.plant-1.Among seven parents,Lal-silbilati,Lalpakri and Ausha were good combiners for the characters,main stem height.plant-1,main stem leaves.plant-1andaverage tuber weight.plant-1,respectively.For average tuber weight.plant-1,the crosses Patnae/TPS-67 and Lalpakri/TPS-13 showed significant positive specific combining ability effects.Ausha/TPS-67 showed significant positive specific com-bining ability effects for main stem leaves.plant-1.
基金Tacking key scientific and technical problems in Heilongjiang(GB04B101)
文摘A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promoters and the presence or absence of the β-glucuronidase(gus) reporter gene.These constructs were integrated into potato cv.Spunta by Agrobacterium tumefaciens-mediated transformation.Highest expression of cryV gene,determined by mRNA levels and insect mortality,was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%,respectively,for potato tuber moth(Phthorimaea operculella Zeller) in the transgenic lines.Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene.Bt expression was also facilitated by using the(ocs)3 mas super promoter,whereas the Bt expression regulated by the patatin promoter(tuber-specific) was too low to have any effect upon the mortality of potato tuber moth.These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes.
文摘为研究不同比例明胶/马铃薯淀粉对大豆分离蛋白(soybean protein isolate,SPI)凝胶性能及3D打印性能的影响,该文以SPI、明胶和马铃薯淀粉为原料,通过热诱导的方式制备SPI复合凝胶,并对复合凝胶的水分分布、质构、流变特性、结构、微观结构以及3D打印特性等进行了表征。结果表明,明胶与马铃薯淀粉比例在1∶8~6∶8(质量比,下同)范围内,随着比例增加,自由水比例降低,不易流动水比例升高,明胶与马铃薯淀粉比例为6∶8时,不易流动水比例最高,占比为2.48%。复配凝胶的硬度、内聚性和咀嚼性随着明胶与马铃薯淀粉比例的增加逐渐降低,6∶8时达到最低,质地最为柔软。随着剪切速率升高,凝胶的黏度均下降,样品的G′和G″随着明胶与马铃薯淀粉比例的增加也均呈升高趋势。傅里叶红外光谱结果显示,添加了明胶和马铃薯淀粉的样品在波长3295.44 cm-1处的—OH振动发生红移,氢键作用增强。随着明胶与马铃薯淀粉比例的增加,复配凝胶孔径逐渐减小,明胶与马铃薯淀粉比例为6∶8时,凝胶孔隙分布呈现出最高的均匀性,打印精度最好,高度打印精确度99.60%,直径打印精确度99.92%,且在1 h内未出现明显塌陷。综上所述,明胶/马铃薯淀粉比例为6∶8时,对大豆分离蛋白凝胶3D打印性能的改善最为显著,这为开发蛋白基3D打印油墨提供理论依据。
