目的以RNA干扰抑制血管平滑肌细胞(vascu lar smooth musc le cells,VSMCs)ORC1基因,探讨ORC1基因表达抑制后VSMCs增殖的变化。方法实验设置正常对照组、阴性siRNA组及阳性(ORC1+A、ORC1+B、ORC1+C)siR-NA组。应用W estern b lot检测ORC...目的以RNA干扰抑制血管平滑肌细胞(vascu lar smooth musc le cells,VSMCs)ORC1基因,探讨ORC1基因表达抑制后VSMCs增殖的变化。方法实验设置正常对照组、阴性siRNA组及阳性(ORC1+A、ORC1+B、ORC1+C)siR-NA组。应用W estern b lot检测ORC1基因表达的变化;应用MTT比色试验、3H-TdR掺入试验检测VSMCs增殖的情况。免疫细胞化学染色观察增殖细胞核抗原(proliferating cell nuc lear antigen,PCNA)表达。结果①siRNA转染后,3个阳性siRNA转染组ORC1基因表达水平均降低,尤以第2对阳性siRNA抑制效果最为显著,而空白对照组及阴性对照组间ORC1基因表达水平无显著差异。②siRNA转染使ORC1表达减弱后,VSMCs的MTT吸光度值3、H-TdR掺入量和PCNA表达量均较空白对照组及阴性对照组显著降低。结论RNA干扰介导的ORC1基因沉寂可显著抑制VSMCs增殖。展开更多
Itanium is the first generation product processor based on IA-64 architecture. ORC(Open Research Compil-er )provides an open source IPF(Itanium Processor Family)research compiler infrastructure. We have compiled andru...Itanium is the first generation product processor based on IA-64 architecture. ORC(Open Research Compil-er )provides an open source IPF(Itanium Processor Family)research compiler infrastructure. We have compiled andrun NAS Benchmarks on the Itanium machine. This paper briefly describes the performance of orcc, sgicc and gcc inthe following 3 ways: execution time, compilation time, and executable file size. The results show that orcc has near-ly the same performance as sgicc, which is 2 fold faster over gcc in the aspect of execution time. We also find that evenwith the best-optimized program, the utilization ratio of process resources is no more that 70%.展开更多
文摘目的以RNA干扰抑制血管平滑肌细胞(vascu lar smooth musc le cells,VSMCs)ORC1基因,探讨ORC1基因表达抑制后VSMCs增殖的变化。方法实验设置正常对照组、阴性siRNA组及阳性(ORC1+A、ORC1+B、ORC1+C)siR-NA组。应用W estern b lot检测ORC1基因表达的变化;应用MTT比色试验、3H-TdR掺入试验检测VSMCs增殖的情况。免疫细胞化学染色观察增殖细胞核抗原(proliferating cell nuc lear antigen,PCNA)表达。结果①siRNA转染后,3个阳性siRNA转染组ORC1基因表达水平均降低,尤以第2对阳性siRNA抑制效果最为显著,而空白对照组及阴性对照组间ORC1基因表达水平无显著差异。②siRNA转染使ORC1表达减弱后,VSMCs的MTT吸光度值3、H-TdR掺入量和PCNA表达量均较空白对照组及阴性对照组显著降低。结论RNA干扰介导的ORC1基因沉寂可显著抑制VSMCs增殖。
文摘Itanium is the first generation product processor based on IA-64 architecture. ORC(Open Research Compil-er )provides an open source IPF(Itanium Processor Family)research compiler infrastructure. We have compiled andrun NAS Benchmarks on the Itanium machine. This paper briefly describes the performance of orcc, sgicc and gcc inthe following 3 ways: execution time, compilation time, and executable file size. The results show that orcc has near-ly the same performance as sgicc, which is 2 fold faster over gcc in the aspect of execution time. We also find that evenwith the best-optimized program, the utilization ratio of process resources is no more that 70%.
