The method of PCR RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I...The method of PCR RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I pigs.The results show that the ESR and FSH β genes are the major genes of litter size;the heterozygous genotype for ESR as well as for FSH β locus has a more litter size than others.However,the combined genotype ABDD means a more litter size than other genotypes,the frequencies of the heterozygous genotypes in Sanjiang and Junmu I are lower than in Min which maybe one of the most important reasons for the little litter sizes of those two breeds.展开更多
Reproduction trait plays an important role in pig production. Identification of molecular markers that are linked to litter size may contribute to the genetic development of porcine reproduction traits. In this study,...Reproduction trait plays an important role in pig production. Identification of molecular markers that are linked to litter size may contribute to the genetic development of porcine reproduction traits. In this study, porcine ring finger protein 4 （pRNF4） and Matrix metalloproteinase 9 （pMMP-9） were selected as candidate genes on the basis of their physiological roles in reproduction. Two single-nucleotide polymorphisms （416C〉T in pRNF4 and - 1257G〉A in pMMP-9） that could be detected by PCR restriction fragment length polymorphism （PCR-RFLP） were discovered and tested for statistical associations with litter size traits in three populations. For 416C〉T, TT genotype was associated with a significantly higher （p〈0.05） number of live births than those recorded for CC sows and the additive effect was significant （p〈0.05） in Qingping and Min Pigs populations in later parities. For -1257G〉A, inconsistent results were found in three populations. The results suggested that T allele in pRNF4 gene might confer a high prolificacy in breeding and further studies were needed to confLrm the results.展开更多
本研究旨在分析序列相似性家族135成员A(Family With Sequence Similarity 135 Member A, FAM135A)基因和丝/酪氨酸蛋白激酶3(Serine/Threonine Protein Kinase 3, STK3)基因的多态性位点与山羊产羔数的关联。基于Sequenom MassARRAY SN...本研究旨在分析序列相似性家族135成员A(Family With Sequence Similarity 135 Member A, FAM135A)基因和丝/酪氨酸蛋白激酶3(Serine/Threonine Protein Kinase 3, STK3)基因的多态性位点与山羊产羔数的关联。基于Sequenom MassARRAY SNP技术对FAM135A和STK3基因的4个候选位点进行分型,并分析了云上黑山羊(n=544)、济宁青山羊(n=133)和辽宁绒山羊(n=91)3个群体中4个候选位点的遗传学特征,同时将这些突变位点与云上黑山羊的繁殖性能(包括产羔数、初生窝重和断奶窝重)作了关联分析。结果显示,在不同繁殖力品种(济宁青山羊和辽宁绒山羊)中,FAM135A基因的g.91260230 G>T和g.91261141 G>A位点以及STK3基因的g.16434985 C>T位点基因型的分布存在着极显著差异(P <0.01);而不同繁殖力品种中STK3基因的g.16648187 C>T位点的基因型分布却没有明显差异(P>0.05)。关联分析结果表明,g.91260230 G>T位点的各基因型在云上黑山羊的产羔数、初生窝重和断奶窝重上都没有明显差异(P>0.05);但g.91261141 G>A位点与产羔数和初生窝重显著相关,GG型产羔数和窝重均显著高于AG型(P <0.05);g.16434985 C>T位点TT型的初生窝重显著高于CT型(P <0.05),但该位点的各基因型对山羊的产羔数和断奶窝重并没有显著影响(P>0.05);g.16648187 C>T位点与山羊的产羔数和初生窝重之间都没有显著关联(P>0.05)。综上,本研究发现FAM135A基因的g.91261141 G>A位点是云上黑山羊产羔数与初生窝重选择的潜在遗传标记。STK3基因的g.16434985 C>T位点适合初生窝重的选择。展开更多
文摘The method of PCR RFLP was used to analyze the effects of ESR and FSH β genotypes on the litter size,especially the corresponding changes with the difference of genotype distributions in the Min,Sanjiang and Junmu I pigs.The results show that the ESR and FSH β genes are the major genes of litter size;the heterozygous genotype for ESR as well as for FSH β locus has a more litter size than others.However,the combined genotype ABDD means a more litter size than other genotypes,the frequencies of the heterozygous genotypes in Sanjiang and Junmu I are lower than in Min which maybe one of the most important reasons for the little litter sizes of those two breeds.
基金Supported by the National Natural Science Foundation of China(31301935)Ph.D.Programs Foundation of Ministry of Education of China(20112325120013)
文摘Reproduction trait plays an important role in pig production. Identification of molecular markers that are linked to litter size may contribute to the genetic development of porcine reproduction traits. In this study, porcine ring finger protein 4 （pRNF4） and Matrix metalloproteinase 9 （pMMP-9） were selected as candidate genes on the basis of their physiological roles in reproduction. Two single-nucleotide polymorphisms （416C〉T in pRNF4 and - 1257G〉A in pMMP-9） that could be detected by PCR restriction fragment length polymorphism （PCR-RFLP） were discovered and tested for statistical associations with litter size traits in three populations. For 416C〉T, TT genotype was associated with a significantly higher （p〈0.05） number of live births than those recorded for CC sows and the additive effect was significant （p〈0.05） in Qingping and Min Pigs populations in later parities. For -1257G〉A, inconsistent results were found in three populations. The results suggested that T allele in pRNF4 gene might confer a high prolificacy in breeding and further studies were needed to confLrm the results.
