Objective:To evaluate the efficacy of entecavir and adefovir dipivoxil on HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B with the method of Meta analysis.Methods:We searched PUBMED,EMBASE,CNKI (C...Objective:To evaluate the efficacy of entecavir and adefovir dipivoxil on HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B with the method of Meta analysis.Methods:We searched PUBMED,EMBASE,CNKI (China National Knowledge Infrastructure),the Cochrane Central Register of Controlled Trials and the Cochrane Database of Systematic Reviews with reference to all data documented before May 2010.The dosage of entecavir and adefovir dipivoxil was 0.5 mg/d and 10 mg/d,respectively.Heterogeneity was examined by Chi-square test,the relative risk calculated and forest plot drawn.Rates of undetected serum HBV DNA,serum alanine aminotransferase (ALT) normalization,HBeAg clearance,HBeAg seroconversion and adverse effect occurrence were analyzed.Results:Six articles were included,which fit well in with this study.Meta analysis showed that the rate of undetected serum HBV DNA(P=0.000 2 at week 12,P=0.002 at week 48)and that of serum ALT normalization(P=0.04 at week 12,P=0.008 at week 48)in the entecavir group were higher than those in the adefovir dipivoxil group.However,no statistic significance existed between the two groups in the rate of HBeAg clearance (P=0.17),the rate of HBeAg seroconversion(P=0.53)or the rate of adverse effect occurrence(P=0.92)at week 48.Conclusion:Entecavir was superior to adefovir dipivoxil in decreasing serum HBV DNA and normalizing serum ALT in the HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B.展开更多
Antiserum against PreS2 peptide was raised with a synthetic polypeptide from the rabbits.The anti-preS2 antibody and polymerized human serum albumin were used as reagents in aradioimmunoassay to detect preS2 and polym...Antiserum against PreS2 peptide was raised with a synthetic polypeptide from the rabbits.The anti-preS2 antibody and polymerized human serum albumin were used as reagents in aradioimmunoassay to detect preS2 and polymerized human serum albumin bindingactivity respectively. Both were absent in patients with hepatitis A or HBsAg negative chronic liver di-seases. In biopsy - proven patients with chronic active hepatitis (CAH)B, prevalences of bothmarkers were significantly higher at exacerbation that at remission stage of the disease, and so werein CAH than in chronic asymptomatic HBV carrier (AsC) with normal histology. Besides, the pre-valences were significantly higher in HBeAg positive group than in anti-HBe positive group.However, the polymerized human serum albumin binding activity and the preS2 were undoubtedlynot the same, as the prevalence of the latter was only 56.7% of the former.展开更多
Objective: The percentages of Th0, Th1, Th2 cells among single CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMC) of chronically HBV-infected individuals were investigated by determining the expr...Objective: The percentages of Th0, Th1, Th2 cells among single CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMC) of chronically HBV-infected individuals were investigated by determining the expression of interleukin-4 (IL-4) and interferon-γ (IFN-γ), and the role of Th1/Th2 cytokines in chronic HBV-infection was analyzed. Methods: PBMCs from chronically HBV-infected individuals were separated routinely, stimulated by PMA, ionomycin and monensin, and the production of IL-4 and IFN-γ by CD4+ T cells in PBMC was determined by fluorescent activated cell sorting (FACS) analysis. Results: The percentages of IFN-γ-producing T cells and IL-4-producing T cells among the CD4+ cells ranged from 2. 3% to 18. 6% and from 0. 9% to 9.2% respectively in non-infected individuals. The majority of CD4+ T cells from PBMCs in chronically HBV-infected individuals were Th0 cells. The percentage of Th1 cells increased significantly with the hepatic iflammation activity. The percentage of Th1 cells in advanced stage of chronic hepatitis B was higher than that in stable stage of chronic hepatitis B. The percentage of Th2 cells in CD4+ T cells from HBV-infected individuals did not differ significantly, but higher than that from controls Conclusion: The results indicate that Th1 phenotype cytokines were associated with hepatic inflammation activity of chronic hepatitis B and Th2 cells may be associated with the chronicity of HBV infection.展开更多
Objective: To establish the relationship between hepatic inflammation activity and Th1/Th2 phenotype cytokines (IFN-γ, IL-4) and to investigate the fore of these cytokines as immunoregulator in inflammation activity ...Objective: To establish the relationship between hepatic inflammation activity and Th1/Th2 phenotype cytokines (IFN-γ, IL-4) and to investigate the fore of these cytokines as immunoregulator in inflammation activity of chronic hepatitis B. Methods: The expression of the mRNA of IL-4 and IFN-γin periphery blood monocytes (PBMC) in patients with chronic hepatitis B was determined by reverse transcription polymena chain reaction (RT-PCR) test. Results: mHNA of neither IL-4 nor IFN-γ was detected in quiescent stage, and the expression rate of IFN-γ mRNA was conspicuously higher in active stage than in convalescent stage (P < 0.05, 100% vs 50% ), while the reverse is true for the expression rate of IL-4 mrNA in convalescent stage (P < 0.05, 87% vs 13%; ). Conclusion: In patients with chronic hepatitis B, IFN-γand IL-2 serve as positive immunoregulatory cytokines, and IL-4 serves as a negative immunoregulator in HBV pathogenesis. These results indicate that Th1 phenotype cytokines up-regulate and Th2 phenotype cytokines down-regulate hepatic inflammation in chronic hepatitis B.展开更多
Objective:To evaluate the antiviral activity of the alcohol extract of Styela plicata on DHBV (duck hepatitis B virus) in vivo. Methods: Guangzhou-Sheldrake ducklings congenitally infected with DHBV were assigned to r...Objective:To evaluate the antiviral activity of the alcohol extract of Styela plicata on DHBV (duck hepatitis B virus) in vivo. Methods: Guangzhou-Sheldrake ducklings congenitally infected with DHBV were assigned to receive the alcohol extract of Styela plicata or lamivudine for 30 consecutive days. The DHBV DNA of sera was detected by RT-PCR. and the histological analysis of duckling liver was evaluated. Results:Thirty days after therapy,histological analysis of duckling liver showed that the ducklings receiving the alcohol extract of Styela plicata or lamivudine exhibited catabatic status in the degree of liver cell degeneration and inflammation compared with the ducklings receiving normal diet. DHBV DNA of sera from alcohol extract of Styela plicata-treated ducklings and lamivudine-treated ducklings all produced significantly lower levels compared with ducklings receiving normal diet (P<0. 01 ). Although these treatment groups all exhibited a rebound phenomenon 10 d after withdrawal of medication, they still exhibited a significant lower level of serum DHBV DNA compared with the control group responded to normal diet (P<0. 05, P<0. 01). Conclusion:Styela plicata may be an effective antiviral medicine in treating chronic hepatitis B. The data of this experiment will be valuable in studying the therapeutic role and the potential therapeutic mechanism of Styela plicata.展开更多
目的:分析HBV感染不同阶段患者外周血T淋巴细胞中PD-1(program death factor-1)分子的基因定量表达情况,探讨PD-1表达水平与血清学指标的相关性。方法:采集并筛选HLA-A2阳性的31例慢性乙型肝炎(CHB)患者、9例急性恢复期乙型肝炎患者(AHB...目的:分析HBV感染不同阶段患者外周血T淋巴细胞中PD-1(program death factor-1)分子的基因定量表达情况,探讨PD-1表达水平与血清学指标的相关性。方法:采集并筛选HLA-A2阳性的31例慢性乙型肝炎(CHB)患者、9例急性恢复期乙型肝炎患者(AHB)、15例乙肝相关性肝硬化患者(LC)和10例健康献血者的外周血,实时荧光定量PCR分析总T细胞内PD-1的mRNA水平,同时测定其配体PD-L1、PD-L2在外周血单个核细胞(PBMCs)中的表达量;酶联免疫吸附试验(ELISA)检测HBV血清标记物水平,荧光定量PCR检测血清HBV DNA载量,同时进行肝功能检测。结果:以各基因在正常对照组的定量表达水平为基础值,HBeAg阳性CHB组T细胞内PD-1和PBMCs内PD-L1的mRNA表达水平明显升高,与HBeAg阴性CHB组和AHB组比较均有显著性差异,但与LC组比较无统计学意义(P>0.05)。外周血PBMCs内PD-L2的mRNA水平在各实验组中无明显差异(P均>0.05)。在所有检测的CHB患者中,外周血T细胞内PD-1表达水平在高病毒载量组(HBV DNA≥107拷贝/ml)明显高于低病毒载量组(HBV DNA<107拷贝/ml)和正常对照组,且与血清HBV病毒载量成正相关(r=0.41,P<0.01),但与血清ALT水平无明显相关性。结论:外周血T细胞中PD-1基因水平在慢性乙型肝炎患者组,尤其是在HBeAg阳性组和高病毒载量组明显上调,提示T细胞高表达的PD-1可能通过与其配体PD-L1作用而抑制T细胞免疫应答,并导致病毒持续感染。展开更多
文摘Objective:To evaluate the efficacy of entecavir and adefovir dipivoxil on HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B with the method of Meta analysis.Methods:We searched PUBMED,EMBASE,CNKI (China National Knowledge Infrastructure),the Cochrane Central Register of Controlled Trials and the Cochrane Database of Systematic Reviews with reference to all data documented before May 2010.The dosage of entecavir and adefovir dipivoxil was 0.5 mg/d and 10 mg/d,respectively.Heterogeneity was examined by Chi-square test,the relative risk calculated and forest plot drawn.Rates of undetected serum HBV DNA,serum alanine aminotransferase (ALT) normalization,HBeAg clearance,HBeAg seroconversion and adverse effect occurrence were analyzed.Results:Six articles were included,which fit well in with this study.Meta analysis showed that the rate of undetected serum HBV DNA(P=0.000 2 at week 12,P=0.002 at week 48)and that of serum ALT normalization(P=0.04 at week 12,P=0.008 at week 48)in the entecavir group were higher than those in the adefovir dipivoxil group.However,no statistic significance existed between the two groups in the rate of HBeAg clearance (P=0.17),the rate of HBeAg seroconversion(P=0.53)or the rate of adverse effect occurrence(P=0.92)at week 48.Conclusion:Entecavir was superior to adefovir dipivoxil in decreasing serum HBV DNA and normalizing serum ALT in the HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B.
文摘Antiserum against PreS2 peptide was raised with a synthetic polypeptide from the rabbits.The anti-preS2 antibody and polymerized human serum albumin were used as reagents in aradioimmunoassay to detect preS2 and polymerized human serum albumin bindingactivity respectively. Both were absent in patients with hepatitis A or HBsAg negative chronic liver di-seases. In biopsy - proven patients with chronic active hepatitis (CAH)B, prevalences of bothmarkers were significantly higher at exacerbation that at remission stage of the disease, and so werein CAH than in chronic asymptomatic HBV carrier (AsC) with normal histology. Besides, the pre-valences were significantly higher in HBeAg positive group than in anti-HBe positive group.However, the polymerized human serum albumin binding activity and the preS2 were undoubtedlynot the same, as the prevalence of the latter was only 56.7% of the former.
文摘Objective: The percentages of Th0, Th1, Th2 cells among single CD4+ T cells isolated from the peripheral blood mononuclear cells (PBMC) of chronically HBV-infected individuals were investigated by determining the expression of interleukin-4 (IL-4) and interferon-γ (IFN-γ), and the role of Th1/Th2 cytokines in chronic HBV-infection was analyzed. Methods: PBMCs from chronically HBV-infected individuals were separated routinely, stimulated by PMA, ionomycin and monensin, and the production of IL-4 and IFN-γ by CD4+ T cells in PBMC was determined by fluorescent activated cell sorting (FACS) analysis. Results: The percentages of IFN-γ-producing T cells and IL-4-producing T cells among the CD4+ cells ranged from 2. 3% to 18. 6% and from 0. 9% to 9.2% respectively in non-infected individuals. The majority of CD4+ T cells from PBMCs in chronically HBV-infected individuals were Th0 cells. The percentage of Th1 cells increased significantly with the hepatic iflammation activity. The percentage of Th1 cells in advanced stage of chronic hepatitis B was higher than that in stable stage of chronic hepatitis B. The percentage of Th2 cells in CD4+ T cells from HBV-infected individuals did not differ significantly, but higher than that from controls Conclusion: The results indicate that Th1 phenotype cytokines were associated with hepatic inflammation activity of chronic hepatitis B and Th2 cells may be associated with the chronicity of HBV infection.
文摘Objective: To establish the relationship between hepatic inflammation activity and Th1/Th2 phenotype cytokines (IFN-γ, IL-4) and to investigate the fore of these cytokines as immunoregulator in inflammation activity of chronic hepatitis B. Methods: The expression of the mRNA of IL-4 and IFN-γin periphery blood monocytes (PBMC) in patients with chronic hepatitis B was determined by reverse transcription polymena chain reaction (RT-PCR) test. Results: mHNA of neither IL-4 nor IFN-γ was detected in quiescent stage, and the expression rate of IFN-γ mRNA was conspicuously higher in active stage than in convalescent stage (P < 0.05, 100% vs 50% ), while the reverse is true for the expression rate of IL-4 mrNA in convalescent stage (P < 0.05, 87% vs 13%; ). Conclusion: In patients with chronic hepatitis B, IFN-γand IL-2 serve as positive immunoregulatory cytokines, and IL-4 serves as a negative immunoregulator in HBV pathogenesis. These results indicate that Th1 phenotype cytokines up-regulate and Th2 phenotype cytokines down-regulate hepatic inflammation in chronic hepatitis B.
基金Supported by the grants from the Social Development Program of Department of Science and Technology of Guangdong Province (2004B30101009).
文摘Objective:To evaluate the antiviral activity of the alcohol extract of Styela plicata on DHBV (duck hepatitis B virus) in vivo. Methods: Guangzhou-Sheldrake ducklings congenitally infected with DHBV were assigned to receive the alcohol extract of Styela plicata or lamivudine for 30 consecutive days. The DHBV DNA of sera was detected by RT-PCR. and the histological analysis of duckling liver was evaluated. Results:Thirty days after therapy,histological analysis of duckling liver showed that the ducklings receiving the alcohol extract of Styela plicata or lamivudine exhibited catabatic status in the degree of liver cell degeneration and inflammation compared with the ducklings receiving normal diet. DHBV DNA of sera from alcohol extract of Styela plicata-treated ducklings and lamivudine-treated ducklings all produced significantly lower levels compared with ducklings receiving normal diet (P<0. 01 ). Although these treatment groups all exhibited a rebound phenomenon 10 d after withdrawal of medication, they still exhibited a significant lower level of serum DHBV DNA compared with the control group responded to normal diet (P<0. 05, P<0. 01). Conclusion:Styela plicata may be an effective antiviral medicine in treating chronic hepatitis B. The data of this experiment will be valuable in studying the therapeutic role and the potential therapeutic mechanism of Styela plicata.
