摘要
目的探讨肝组织HBV cccDNA定量对慢性乙型肝炎(乙肝)患者病情的影响。方法应用FQ-PRC法检测42例乙肝患者肝组织HBV cccDNA、肝组织和血清HBV-DNA水平,同时对肝组织行常规病理染色判断肝组织炎症及纤维化程度;SP法检测肝组织中HBsAg、HBcAg表达,化学发光法检测HBV标志物。分析肝组织HBVcccDNA与组织和血清HBV-DNA、肝细胞内HBsAg、HBcAg水平及肝脏炎症、纤维化程度的关系。结果肝组织HBV cccDNA定量与组织及血清HBV-DNA定量呈正相关(r=0.807,P<0.001;r=0.627,P<0.001);与肝组织炎症活动度及纤维化程度无明显相关性;肝组织HBV cccDNA定量与肝细胞内HBsAg表达无明显相关性,而与HB-cAg表达呈正相关(r=0.486,P<0.05)。结论检测肝组织内HBV cccDNA可更精确反映HBV复制程度;对乙肝诊断、抗病毒治疗及选择停药时机具有重要价值。肝组织HBV cccDNA与血清HBV-DNA定量及HBcAg联合检测可指导抗病毒治疗。
Objective To investigate the effect of HBV cccDNA quantitation on the condition of chronic hepatitis B （CHB） patients. Methods The quantity of HBV eceDNA and HBV DNA in the hepatocytes and HBV DNA in the serum of 42 patients with CHB were detected by real-time fluorescence quantitative PCR （FQ-PCR）. The inflammation and fibro- sis degree were estimated by pathological staining. The expression of HBsAg, HBcAg in the hepatocytes were identified by SP method. Correlations between the HBV eceDNA quantitation and other indexes were analyzed. Results The HBV eeeDNA quantitation in the liver tissue were positively correlated to the HBV DNA levels in the liver tissue and sera（ r = 0.807, P 〈 0.001. r = 0.627, P 〈 0.001 ）, and were not correlated to the degree of liver inflammation and fibrosis. The HBV eeeDNA quantitation in the liver tissue were not correlated to the expression of HBsAg, but positively correlated to the expresson of HBeAg （ r = 0.486, P 〈 0.05 ）. Conclusions The quantitation of HBV eeeDNA can reflect the replication of HBV more accurately, and plays important role in the diagnosis, antiviral treatment and withdrawal time selection of CHB. It is more reliable to take the quantification of hepatic tissue HBV cceDNA combining the HBV DNA in the serum and the expression degree of HBcAg as antivirus therapeutic indexes.
出处
《山东医药》
CAS
北大核心
2010年第7期12-14,共3页
Shandong Medical Journal
