Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduce...Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduced plant stature and deficiency in various 7S and 11S subunits,designated as snd1 mutants.Under normal growth conditions,the snd1 mutants showed a severe dwarf phenotype,with plant height of about 25 cm.Compared with wild-type DN47,the mutant snd1 exhibited no obvious morphological differences at the early stage of development.All the snd1 mutants examined had fewer nodes and shorter than normal internodes;the leaves were similar in shape to normal parents,but were dark-green at the mature stage.The flower size was similar to DN47;however,the flowering period was shorter than in the wild-type.Significant variation was noted for protein content,oil content of the seeds and size of seeds(weight of 100 seeds)among 17 snd1 dwarf lines.Genetic analysis indicated that the dwarfism of snd1 was controlled by a single recessive gene.The snd1 dwarf mutant had markedly different dynamic levels of the endogenous hormones gibberellin(GA),brassinosteroid,indole-3-acetic acid and abscisic acid,at the seedling stage.Exogenous GA3 treatment led to recovery of the plant height phenotype of the snd1 mutant;GA3 at 0.1 mm had the largest effect on enhancing plant height.Using molecular markers,snd1 gene was approximately mapped in an interval of 603 kb between markers Satt166 and Satt561 on chromosome 19.Snd1 mutant provided valuable material for hypoallergenic soybean breeding and the snd1 gene might be a novel gene related to plant height in soybean.展开更多
Studies have shown that the three subunits of β-conglycinin are the main potential allergens of soybean sensitive patients.And β-conglycinin has adverse effects on nutrition and food processing.So solation and produ...Studies have shown that the three subunits of β-conglycinin are the main potential allergens of soybean sensitive patients.And β-conglycinin has adverse effects on nutrition and food processing.So solation and production of lines with lowerβ-conglycinin content has been the focus of recent soybean breeding projects.Soybean lines with deficiency in one or all subunits of β-congIycinin have been obtained.An effective and rapid system to identify such mutations will facilitate genetic manipulation of the β-conglycinin subunit composition.Here,two segregating F2 populations were developed from crosses between Cgy-1/cgy-1(CC),anα'-lacking line(△α'),and DongNong 47(DN47),a wild-type(Wt)Chinese soybean cultivar with normal globulin components,and Cgy-2/cgy-2(CB),an a-lacking line(△α),and DN47.These populations were used to estimate linkage among the egy-1(conferring α'-null)and cgy-2(α-null)loci and simple sequence repeat(SSR)markers.Seven SSR markers(Sat_038,Satt243,Sat_307,Sat_109,Sat_231,Sat_108 and Sat_190)were determined to co-scgregate with cgy-1,and six SSR markers(Satt650,Satt671,Sat_418,Sat_170,Satt292 and Sat_324)co-segregated with cgy-2.Linkage maps being composed of seven SSR markers and egy-1 locus,and six SSR markers and the cgy-2 locus were then constructed.It assigned that the egy-1 gene to chromosome 10 at a position between Sat_307 and Sat_231,and the cgy-2 gene to chromosome 20 at a position between Satt650 and Satt671.These markers should enable map-based cloning of the egy-1 and cgy-2 genes.For different subunit-deficiency types[α'-null,α-null and(α'+α)-null types],the two sets of SSR markers could also detect of polymorphism between three normal cultivars and seven related mutant lines.The identification of these markers is great significance to the molecular marker-assisted breeding of soybean/9-conglycinin subunits.展开更多
Soybean(Glycine max)is one of the most important food crops and oil crops in the world.According to the role of sucrose transporter in sugar accumulation,GmTST2.1(Glyma.04G000300)and ZmGIF1 of sugar transport related ...Soybean(Glycine max)is one of the most important food crops and oil crops in the world.According to the role of sucrose transporter in sugar accumulation,GmTST2.1(Glyma.04G000300)and ZmGIF1 of sugar transport related genes were separately overexpressed in the soybean cultivar Heihe 43 from the perspective of regulatory source to library relationship in the study.The function of soluble sugar accumulation in grains layed a theoretical foundation for the cultivation of new varieties of high-yield genetically modified soybeans.The results showed that the height and 100-seed weight of the over-expressed GmTST2.1 gene were increased with 7%and 17.7%and the soluble sugar content was increased with 1.575 times as much as that of the wild-type soybean.The overexpressed ZmGIF1 gene was found to be 10%higher than that of plant height,1.8%higher than that of 100-seed weight and larger seed size and 1.3 times higher than that of soluble sugar content.Biological yields were increased in both GmTST2.1 and ZmGIF1 genes.展开更多
LEAFY COTYLEDON1(LEC1) is a key regulator of seed maturation, which gives embryos the ability to withstand desiccation. In this study, a novel transcription factor that is homologous to LEC1 in soybean(Glycine max) wa...LEAFY COTYLEDON1(LEC1) is a key regulator of seed maturation, which gives embryos the ability to withstand desiccation. In this study, a novel transcription factor that is homologous to LEC1 in soybean(Glycine max) was isolated from Dongnong 50 by homologous cloning and was named as GmLEC1-A(GenBank accession number: MF681785). Sequence analysis showed that GmLEC1-A contained conserved B regions, which were functional domains of H4 factor. The relative expression level of GmLEC1-A was the highest in seeds of the soybean cultivar Dongnong 50. To verify the function of GmLEC1-A, ectopic expression of Arabidopsis thaliana and over-expression of soybean plants were generated. In Arabidopsis, the expression of GmLEC1-A restored the tolerance of lec1 mutant to seed drying, indicating that GmLEC1-A was a functional homolog of LEC1,and it might regulate the maturation phase of seed development. In soybean, over-expression of GmLEC1-A caused slower growth and lower germination rates as compared to that of wild-type soybeans. Furthermore, over-expression of GmLEC1-A seemed to increase the level of endogenous abscisic acid(ABA) at the germination stage. These results suggested that GmLEC1-A had a function in regulating ABA content at the germination stage.展开更多
A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference ...A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.展开更多
背景与目的:弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)分子遗传学特征和患者治疗前^(18)F-FDG PET/CT检查评估的SUV_(max)值均与患者预后密切相关,但两者的关系及其与R-CHOP治疗方案治疗反应的相关性尚不清楚。本研究...背景与目的:弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)分子遗传学特征和患者治疗前^(18)F-FDG PET/CT检查评估的SUV_(max)值均与患者预后密切相关,但两者的关系及其与R-CHOP治疗方案治疗反应的相关性尚不清楚。本研究旨在分析DLBCL分子遗传学特征与治疗前经^(18)F-FDG PET/CT检测的SUV_(max)值的关系及其与临床病理学特征、R-CHOP治疗反应的相关性。方法:回顾性收集复旦大学附属肿瘤医院2022-2023年同时经淋巴瘤481基因DNA panel二代测序(next-generation sequencing,NGS)和治疗前经PET/CT检查的DLBCL患者225例,本研究通过复旦大学附属肿瘤医院医学伦理委员会的审查(伦理批号:050432-4-2307E)并获得患者知情同意;除基因突变特征外,同时收集荧光原位杂交法检测的BCL2、BCL6和MYC基因易位情况;另收集该组病例的临床病理学参数以及经R-CHOP治疗后的PET/CT检查结果。结果:总计191例DLBCL患者纳入最终分析,重要基因MYD88突变、TP53突变、CDKN2A/2B拷贝数异常、CD79B突变发生率分别为24.6%、27.2%、32.5%和16.8%。治疗前SUV_(max)值范围是5.10~63.10(24.44±10.70,中位22.80)。MYD88L265P突变型DLBCL的治疗前SUV_(max)值显著高于MYD88野生型DLBCL(P=0.039),SUV_(max)值与DLBCL其他基因变异类型包括TP53突变、CDKN2A/B拷贝数减少、CD79B突变、KMT2D突变、TNFAIP3突变、B2M突变、EZH2突变、BTG1/2突变、CREBBP突变、MYC、BCL2、BCL6基因重排之间无显著的相关性。治疗前高SUV_(max)值与高血清乳酸脱氢酶(lactate dehydrogenase,LDH)水平(P=0.012)及非生发中心(non-germinal center B-cell-like,non-GCB)亚型显著相关(P=0.040),但与R-CHOP治疗反应无显著的相关性(P=0.714)。DLBCL中TP53基因突变与R-CHOP治疗反应差显著相关(P=0.001),是R-CHOP治疗后非完全代谢缓解的独立预测因子。联合TP53基因突变、Ann Arbor分期、国际预后指数(International Prognostic Index,IPI)及血清LDH水平能够更好地预测患者对R-CHOP治疗的反应。结论:在DLBCL中,MYD88L265P突变型患者具有较高的治疗前SUV_(max)值。DLBCL治疗前SUV_(max)值与R-CHOP治疗反应无关,而TP53基因突变与R-CHOP治疗反应差显著相关,并且是独立预测因子。TP53基因突变联合临床病理学参数可更好地预测R-CHOP治疗反应。关于各基因变异特征及SUV_(max)值与患者预后的关系尚需作进一步随访研究。展开更多
Sodium cocoyl glycinate(SCG),an environmentally friendly anionic amino acid surfactant,is widely used in daily chemical products as an upgraded alternative to traditional surfactants.In this study,crude Camellia oleif...Sodium cocoyl glycinate(SCG),an environmentally friendly anionic amino acid surfactant,is widely used in daily chemical products as an upgraded alternative to traditional surfactants.In this study,crude Camellia oleifera saponin(COS)was purified using AB-8 macroporous adsorption resin,and its composition and structure were analyzed.The effects of different mole fractions of COS(αCOS)on surface tension(γ),oil-water interfacial tension(IFT),emulsification,and foam properties of COS-SCG binary mixed systems were investigated in mixtures of SCG with purified COS.The stability ofγand foamability under diverse environmental conditions were also discussed.The results indicated that the COS-SCG system exhibited remarkable surface-active synergism.The minimum critical micelle concentration(cmc)of the mixed system was lower than that of SCG,and adding a small mole fraction of COS(1%-2%)induced a synergistic reduction ofγ.Specifically,the cmc andγwere 2.50×10-4 mol/L and 23.1 mN/m forαCOS=1%,respectively.The system exhibited exceptional IFT reduction capacity,achieving a minimum value of 1.42 mN/m atαCOS=10%.The mixed system reached a foaming volume(atαCOS=50%)and foam stability(atαCOS=75%)were 51.0 mL and 97.37%,respectively.Microscopic analysis further confirmed these outstanding foam properties.Moreover,the COS-SCG system displayed reducedγwith enhanced foaming volume under elevated temperatures(35-75℃)and salinity(0-20 g/L).However,acidic conditions and hard water compromised bothγstability and foamability.展开更多
The effects of the deficiency of the allergenicα-subunit on soybean amino acid(AA)composition were studied using the cultivar Dongnong 47(DN47)as a genetic background.The near-isogenic line(NIL)NIL-DN47-Δα of DN47,...The effects of the deficiency of the allergenicα-subunit on soybean amino acid(AA)composition were studied using the cultivar Dongnong 47(DN47)as a genetic background.The near-isogenic line(NIL)NIL-DN47-Δα of DN47,with an introgression of theα-null trait allele from the high protein donor parent RiB,was created by marker assisted background selection and used to investigate the AA content and nutritional quality.The contents of crude protein,the total AAs,the total essential amino acids(EAAs)and sulfur-containing(Met and Cys)AAs increased by 4.11%,4.16%,5.20% and 11.96%,respectively in NIL-DN47-Δα compared with DN47.Analyses of the total EAAs(TEAAs)and the EAA index(EAAI)revealed that both parameters in NIL-DN47-Δα were higher than those in DN47.The null-allele of theα-subunit positively affected the AA scores.The quantitative changes in free AAs(FAAs)in the developing seeds of NIL-DN47-Δα and DN47 were compared as of 15 days after flowering(DAF)until maturity.The results showed that the total FAA content in NIL-DN47-Δα was significantly higher than that in the DN47 throughout the late maturation stage(40-60 DAF)of seeds.The high concentration of the FAAs in cgy-2 mutant seeds was a consequence of the high rates of synthesis and/or accumulation of individual FAAs during seed maturation where 25 DAF was an important turning point in the accumulation of the FAAs.The FAA contents of single soybeanα-null,double(α+α′)and triple(α+α′+group I)-null mutant combination lines were investigated.In all of these combinations,introduction of the cgy-2 gene invariably raised the FAA content of mature seeds above that of the DN47.In summary,the enhanced protein quality in cgy-2 mutants resulted from several factors.(1)There was a general increase in the contents of most AAs and FAAs in NIL-DN47-Δα.(2)The induced synthesis of free Arg contributed effectively to the high FAAs of various storage-protein-deficiency mutants.For example,in the S2(null α,group I),the free Arg content was seven times as much as that of DN47,accounting for more than half of the total FAA content in the seed.(3)The increase of sulfur-containing AAs in theα-null type NIL mainly resulted from elevated Met content.These data suggested that the cgy-2 mutation might improve the protein quality of soybean seeds and that lacked of the allergenicα-subunit resulted in increased the FAA content.展开更多
In view of the difference in coordination capacity of the glycine ion(Gly−),a selective leaching process for treating with spent lithium-ion batteries(LIBs)in the alkaline glycinate system was proposed.The effects of ...In view of the difference in coordination capacity of the glycine ion(Gly−),a selective leaching process for treating with spent lithium-ion batteries(LIBs)in the alkaline glycinate system was proposed.The effects of retention time,leaching temperature,concentration of glycine ligand,liquid-solid ratio(L/S),pH,stirring speed,and H_(2)O_(2) dosage on the leaching efficiency of valuable metals and the dissolution of impurities were investigated.When the spent LIBs were leached in 3 mol/L glycine aqueous solution with pH of 8,L/S of 5 mL:1 g and H_(2)O_(2) dosage of 5 vol.%at 90℃and stirring speed of 400 r/min for 3 h,lithium,cobalt,nickel,and manganese recoveries were 96.31%,83.18%,91.56%,and 31.16%,respectively,but Ca,Al,Fe,and Cu were almost insoluble.Meanwhile,the kinetic study showed that the activation energies for the leaching of Li,Co,Ni,and Mn were all in the range of 45−61 kJ/mol.The results indicate that the leaching process is all controlled by chemical reactions.展开更多
基金Supported by the Ministry of Science and Technology of China(2016YFD0100500)Funding from Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)+4 种基金the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)National Natural Science Foundation of China(31801386)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-2018158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)
文摘Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduced plant stature and deficiency in various 7S and 11S subunits,designated as snd1 mutants.Under normal growth conditions,the snd1 mutants showed a severe dwarf phenotype,with plant height of about 25 cm.Compared with wild-type DN47,the mutant snd1 exhibited no obvious morphological differences at the early stage of development.All the snd1 mutants examined had fewer nodes and shorter than normal internodes;the leaves were similar in shape to normal parents,but were dark-green at the mature stage.The flower size was similar to DN47;however,the flowering period was shorter than in the wild-type.Significant variation was noted for protein content,oil content of the seeds and size of seeds(weight of 100 seeds)among 17 snd1 dwarf lines.Genetic analysis indicated that the dwarfism of snd1 was controlled by a single recessive gene.The snd1 dwarf mutant had markedly different dynamic levels of the endogenous hormones gibberellin(GA),brassinosteroid,indole-3-acetic acid and abscisic acid,at the seedling stage.Exogenous GA3 treatment led to recovery of the plant height phenotype of the snd1 mutant;GA3 at 0.1 mm had the largest effect on enhancing plant height.Using molecular markers,snd1 gene was approximately mapped in an interval of 603 kb between markers Satt166 and Satt561 on chromosome 19.Snd1 mutant provided valuable material for hypoallergenic soybean breeding and the snd1 gene might be a novel gene related to plant height in soybean.
基金Supported by the Ministry of Science and Technology of China(2016YFD0I00500)Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)+4 种基金the National Natural Science Foundation of China(31801386,31371650 and 31071440)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-20I8158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)。
文摘Studies have shown that the three subunits of β-conglycinin are the main potential allergens of soybean sensitive patients.And β-conglycinin has adverse effects on nutrition and food processing.So solation and production of lines with lowerβ-conglycinin content has been the focus of recent soybean breeding projects.Soybean lines with deficiency in one or all subunits of β-congIycinin have been obtained.An effective and rapid system to identify such mutations will facilitate genetic manipulation of the β-conglycinin subunit composition.Here,two segregating F2 populations were developed from crosses between Cgy-1/cgy-1(CC),anα'-lacking line(△α'),and DongNong 47(DN47),a wild-type(Wt)Chinese soybean cultivar with normal globulin components,and Cgy-2/cgy-2(CB),an a-lacking line(△α),and DN47.These populations were used to estimate linkage among the egy-1(conferring α'-null)and cgy-2(α-null)loci and simple sequence repeat(SSR)markers.Seven SSR markers(Sat_038,Satt243,Sat_307,Sat_109,Sat_231,Sat_108 and Sat_190)were determined to co-scgregate with cgy-1,and six SSR markers(Satt650,Satt671,Sat_418,Sat_170,Satt292 and Sat_324)co-segregated with cgy-2.Linkage maps being composed of seven SSR markers and egy-1 locus,and six SSR markers and the cgy-2 locus were then constructed.It assigned that the egy-1 gene to chromosome 10 at a position between Sat_307 and Sat_231,and the cgy-2 gene to chromosome 20 at a position between Satt650 and Satt671.These markers should enable map-based cloning of the egy-1 and cgy-2 genes.For different subunit-deficiency types[α'-null,α-null and(α'+α)-null types],the two sets of SSR markers could also detect of polymorphism between three normal cultivars and seven related mutant lines.The identification of these markers is great significance to the molecular marker-assisted breeding of soybean/9-conglycinin subunits.
基金Supported by Heilongjiang Provincial Project(Topic JC2018007,GX17B002,C2018016,GJ2018GJ0098)Chinese National Natural Science Foundation(31671717)+1 种基金the Postdoctoral Fund in Heilongjiang Province(LBH-Z15017,LBH-Q17015)the National Project(CARS-04-PS04)。
文摘Soybean(Glycine max)is one of the most important food crops and oil crops in the world.According to the role of sucrose transporter in sugar accumulation,GmTST2.1(Glyma.04G000300)and ZmGIF1 of sugar transport related genes were separately overexpressed in the soybean cultivar Heihe 43 from the perspective of regulatory source to library relationship in the study.The function of soluble sugar accumulation in grains layed a theoretical foundation for the cultivation of new varieties of high-yield genetically modified soybeans.The results showed that the height and 100-seed weight of the over-expressed GmTST2.1 gene were increased with 7%and 17.7%and the soluble sugar content was increased with 1.575 times as much as that of the wild-type soybean.The overexpressed ZmGIF1 gene was found to be 10%higher than that of plant height,1.8%higher than that of 100-seed weight and larger seed size and 1.3 times higher than that of soluble sugar content.Biological yields were increased in both GmTST2.1 and ZmGIF1 genes.
基金Supported by the Creative Research Groups of Heilongjiang Province of China(JC2016004)the National Key R&D Program of China(2016YFD0100201-21)+1 种基金Project of Outstanding Academic Leaders in Harbin(2015RQXXJ018)China Agriculture Collaborative Creation Research System of Miscellaneous Grain Crops
文摘LEAFY COTYLEDON1(LEC1) is a key regulator of seed maturation, which gives embryos the ability to withstand desiccation. In this study, a novel transcription factor that is homologous to LEC1 in soybean(Glycine max) was isolated from Dongnong 50 by homologous cloning and was named as GmLEC1-A(GenBank accession number: MF681785). Sequence analysis showed that GmLEC1-A contained conserved B regions, which were functional domains of H4 factor. The relative expression level of GmLEC1-A was the highest in seeds of the soybean cultivar Dongnong 50. To verify the function of GmLEC1-A, ectopic expression of Arabidopsis thaliana and over-expression of soybean plants were generated. In Arabidopsis, the expression of GmLEC1-A restored the tolerance of lec1 mutant to seed drying, indicating that GmLEC1-A was a functional homolog of LEC1,and it might regulate the maturation phase of seed development. In soybean, over-expression of GmLEC1-A caused slower growth and lower germination rates as compared to that of wild-type soybeans. Furthermore, over-expression of GmLEC1-A seemed to increase the level of endogenous abscisic acid(ABA) at the germination stage. These results suggested that GmLEC1-A had a function in regulating ABA content at the germination stage.
基金Supported by Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)+3 种基金the National Natural Science Foundation of China(31801386)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-2018158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)
文摘A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.
文摘背景与目的:弥漫性大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)分子遗传学特征和患者治疗前^(18)F-FDG PET/CT检查评估的SUV_(max)值均与患者预后密切相关,但两者的关系及其与R-CHOP治疗方案治疗反应的相关性尚不清楚。本研究旨在分析DLBCL分子遗传学特征与治疗前经^(18)F-FDG PET/CT检测的SUV_(max)值的关系及其与临床病理学特征、R-CHOP治疗反应的相关性。方法:回顾性收集复旦大学附属肿瘤医院2022-2023年同时经淋巴瘤481基因DNA panel二代测序(next-generation sequencing,NGS)和治疗前经PET/CT检查的DLBCL患者225例,本研究通过复旦大学附属肿瘤医院医学伦理委员会的审查(伦理批号:050432-4-2307E)并获得患者知情同意;除基因突变特征外,同时收集荧光原位杂交法检测的BCL2、BCL6和MYC基因易位情况;另收集该组病例的临床病理学参数以及经R-CHOP治疗后的PET/CT检查结果。结果:总计191例DLBCL患者纳入最终分析,重要基因MYD88突变、TP53突变、CDKN2A/2B拷贝数异常、CD79B突变发生率分别为24.6%、27.2%、32.5%和16.8%。治疗前SUV_(max)值范围是5.10~63.10(24.44±10.70,中位22.80)。MYD88L265P突变型DLBCL的治疗前SUV_(max)值显著高于MYD88野生型DLBCL(P=0.039),SUV_(max)值与DLBCL其他基因变异类型包括TP53突变、CDKN2A/B拷贝数减少、CD79B突变、KMT2D突变、TNFAIP3突变、B2M突变、EZH2突变、BTG1/2突变、CREBBP突变、MYC、BCL2、BCL6基因重排之间无显著的相关性。治疗前高SUV_(max)值与高血清乳酸脱氢酶(lactate dehydrogenase,LDH)水平(P=0.012)及非生发中心(non-germinal center B-cell-like,non-GCB)亚型显著相关(P=0.040),但与R-CHOP治疗反应无显著的相关性(P=0.714)。DLBCL中TP53基因突变与R-CHOP治疗反应差显著相关(P=0.001),是R-CHOP治疗后非完全代谢缓解的独立预测因子。联合TP53基因突变、Ann Arbor分期、国际预后指数(International Prognostic Index,IPI)及血清LDH水平能够更好地预测患者对R-CHOP治疗的反应。结论:在DLBCL中,MYD88L265P突变型患者具有较高的治疗前SUV_(max)值。DLBCL治疗前SUV_(max)值与R-CHOP治疗反应无关,而TP53基因突变与R-CHOP治疗反应差显著相关,并且是独立预测因子。TP53基因突变联合临床病理学参数可更好地预测R-CHOP治疗反应。关于各基因变异特征及SUV_(max)值与患者预后的关系尚需作进一步随访研究。
文摘Sodium cocoyl glycinate(SCG),an environmentally friendly anionic amino acid surfactant,is widely used in daily chemical products as an upgraded alternative to traditional surfactants.In this study,crude Camellia oleifera saponin(COS)was purified using AB-8 macroporous adsorption resin,and its composition and structure were analyzed.The effects of different mole fractions of COS(αCOS)on surface tension(γ),oil-water interfacial tension(IFT),emulsification,and foam properties of COS-SCG binary mixed systems were investigated in mixtures of SCG with purified COS.The stability ofγand foamability under diverse environmental conditions were also discussed.The results indicated that the COS-SCG system exhibited remarkable surface-active synergism.The minimum critical micelle concentration(cmc)of the mixed system was lower than that of SCG,and adding a small mole fraction of COS(1%-2%)induced a synergistic reduction ofγ.Specifically,the cmc andγwere 2.50×10-4 mol/L and 23.1 mN/m forαCOS=1%,respectively.The system exhibited exceptional IFT reduction capacity,achieving a minimum value of 1.42 mN/m atαCOS=10%.The mixed system reached a foaming volume(atαCOS=50%)and foam stability(atαCOS=75%)were 51.0 mL and 97.37%,respectively.Microscopic analysis further confirmed these outstanding foam properties.Moreover,the COS-SCG system displayed reducedγwith enhanced foaming volume under elevated temperatures(35-75℃)and salinity(0-20 g/L).However,acidic conditions and hard water compromised bothγstability and foamability.
基金Support by the National Natural Science Foundation of China(31371650,31071440)Northeast Agricultural University Innovation Foundation For Postgraduates(yjscx4042)。
文摘The effects of the deficiency of the allergenicα-subunit on soybean amino acid(AA)composition were studied using the cultivar Dongnong 47(DN47)as a genetic background.The near-isogenic line(NIL)NIL-DN47-Δα of DN47,with an introgression of theα-null trait allele from the high protein donor parent RiB,was created by marker assisted background selection and used to investigate the AA content and nutritional quality.The contents of crude protein,the total AAs,the total essential amino acids(EAAs)and sulfur-containing(Met and Cys)AAs increased by 4.11%,4.16%,5.20% and 11.96%,respectively in NIL-DN47-Δα compared with DN47.Analyses of the total EAAs(TEAAs)and the EAA index(EAAI)revealed that both parameters in NIL-DN47-Δα were higher than those in DN47.The null-allele of theα-subunit positively affected the AA scores.The quantitative changes in free AAs(FAAs)in the developing seeds of NIL-DN47-Δα and DN47 were compared as of 15 days after flowering(DAF)until maturity.The results showed that the total FAA content in NIL-DN47-Δα was significantly higher than that in the DN47 throughout the late maturation stage(40-60 DAF)of seeds.The high concentration of the FAAs in cgy-2 mutant seeds was a consequence of the high rates of synthesis and/or accumulation of individual FAAs during seed maturation where 25 DAF was an important turning point in the accumulation of the FAAs.The FAA contents of single soybeanα-null,double(α+α′)and triple(α+α′+group I)-null mutant combination lines were investigated.In all of these combinations,introduction of the cgy-2 gene invariably raised the FAA content of mature seeds above that of the DN47.In summary,the enhanced protein quality in cgy-2 mutants resulted from several factors.(1)There was a general increase in the contents of most AAs and FAAs in NIL-DN47-Δα.(2)The induced synthesis of free Arg contributed effectively to the high FAAs of various storage-protein-deficiency mutants.For example,in the S2(null α,group I),the free Arg content was seven times as much as that of DN47,accounting for more than half of the total FAA content in the seed.(3)The increase of sulfur-containing AAs in theα-null type NIL mainly resulted from elevated Met content.These data suggested that the cgy-2 mutation might improve the protein quality of soybean seeds and that lacked of the allergenicα-subunit resulted in increased the FAA content.
基金Projects(51974137,52274299)supported by the National Natural Science Foundation of ChinaProject(2023M733190)supported by the China Postdoctoral Science Foundation。
文摘In view of the difference in coordination capacity of the glycine ion(Gly−),a selective leaching process for treating with spent lithium-ion batteries(LIBs)in the alkaline glycinate system was proposed.The effects of retention time,leaching temperature,concentration of glycine ligand,liquid-solid ratio(L/S),pH,stirring speed,and H_(2)O_(2) dosage on the leaching efficiency of valuable metals and the dissolution of impurities were investigated.When the spent LIBs were leached in 3 mol/L glycine aqueous solution with pH of 8,L/S of 5 mL:1 g and H_(2)O_(2) dosage of 5 vol.%at 90℃and stirring speed of 400 r/min for 3 h,lithium,cobalt,nickel,and manganese recoveries were 96.31%,83.18%,91.56%,and 31.16%,respectively,but Ca,Al,Fe,and Cu were almost insoluble.Meanwhile,the kinetic study showed that the activation energies for the leaching of Li,Co,Ni,and Mn were all in the range of 45−61 kJ/mol.The results indicate that the leaching process is all controlled by chemical reactions.
