Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera⁃py ...Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera⁃py for ischemic peripheral nerve injuries,this study utilized glucose-modified near-infrared-II(NIR-II)quantum dots(QDs)to label adipose-derived stem cell exosomes(QDs-ADSC-Exos),enabling long-term in vivo NIR-II imaging of exosome treatment for ischemic peripheral nerve damage.Experimental results confirmed that QDs can be used for non-invasive in vitro labeling of exosomes,with QDs-ADSC-Exos exhibiting strong fluorescence signals in the NIR-II window and demonstrating favorable NIR-II imaging characteristics in vivo.Notably,QDsADSC-Exos showed accumulation at the site of nerve injury in cases of ischemic peripheral nerve damage.Func⁃tional neurological assessments indicated that QDs-ADSC-Exos effectively promoted neural regeneration.This study highlights the potential of exosomes in treating ischemic peripheral nerve injuries and elucidates the spatio⁃temporal characteristics of exosome therapy,providing objective evidence for the further optimization of exosomebased treatment protocols.展开更多
Exosomes,ubiquitously present in body fluids,serve as non-invasive biomarkers for disease diagnosis,monitoring,and treatment.As intercellular messengers,exosomes encapsulate a rich array of proteins,nucleic acids,and ...Exosomes,ubiquitously present in body fluids,serve as non-invasive biomarkers for disease diagnosis,monitoring,and treatment.As intercellular messengers,exosomes encapsulate a rich array of proteins,nucleic acids,and metabolites,although most studies have primarily focused on proteins and RNA.Recently,exosome metabolomics has demonstrated clinical value and potential advantages in disease detection and pathophysiology,despite significant challenges,particularly in exosome isolation and metabolite detection.This review discusses the significant technical challenges in exosome isolation and metabolite detection,highlighting the advancements in these areas that support the clinical application of exosome metabolomics,and illustrates the potential of exosomal metabolites from various body fluids as biomarkers for early disease diagnosis and treatment.展开更多
目的:观察IL-12与IL-18联合刺激对树突状细胞(dendritic cell,DC)分泌的exosome(DC derived exosome,Dex)活性的影响,为探索高效的exosome肿瘤疫苗奠定基础。方法:取正常健康人外周血单个核细胞诱导培养DCs,分别以IL-12、IL-18或IL-12+I...目的:观察IL-12与IL-18联合刺激对树突状细胞(dendritic cell,DC)分泌的exosome(DC derived exosome,Dex)活性的影响,为探索高效的exosome肿瘤疫苗奠定基础。方法:取正常健康人外周血单个核细胞诱导培养DCs,分别以IL-12、IL-18或IL-12+IL-18联合刺激DC,并设空白对照组、T细胞对照组,分别提取各组的Dex,Western blotting检测Dex中HLA-DR、CD83的表达,流式细胞仪检测CD54、CD80及CD86的表达;MTT法检测Dex刺激T细胞增殖的作用,ELISA法测定T细胞IFN-γ的分泌量。结果:IL-12、IL-18、联合组、空白组的Dex中均有HLA-DR、CD83蛋白表达;联合组Dex的CD54(323.67±44.06 vs 246.17±31.91、236.33±33.87、167.67±28.73,P<0.05)、CD80(406.37±39.18 vs 331.67±36.15、335.67±41.38、260.00±35.58,P<0.05)及CD86(390.50±38.06 vs 314.33±36.64、319.00±33.10、246.83±30.55,P<0.05)表达均高于IL-12组、IL-18组及空白组;联合组刺激T细胞增殖高于IL-12组、IL-18组及空白组、T细胞组(1.98±0.31vs 1.55±0.23、1.57±0.21、1.10±0.18、0.53±0.09,P<0.05);联合组刺激T细胞分泌IFN-γ水平高于IL-12组、IL-18组及空白组、T细胞组(436.67±61.80 vs 295.04±40.25、358.18±55.77、225.00±36.44、139.50±17.63,P<0.05)。结论:IL-12与IL-18联合刺激能增加Dex表达CD54、CD80及CD86,促进Dex刺激的T细胞的增殖及其IFN-γ的分泌。展开更多
基金Supported by the National Natural Science Foundation of China(82371373,W2412120)the Shanghai Natural Science Foundation(21ZR1436100).
文摘Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera⁃py for ischemic peripheral nerve injuries,this study utilized glucose-modified near-infrared-II(NIR-II)quantum dots(QDs)to label adipose-derived stem cell exosomes(QDs-ADSC-Exos),enabling long-term in vivo NIR-II imaging of exosome treatment for ischemic peripheral nerve damage.Experimental results confirmed that QDs can be used for non-invasive in vitro labeling of exosomes,with QDs-ADSC-Exos exhibiting strong fluorescence signals in the NIR-II window and demonstrating favorable NIR-II imaging characteristics in vivo.Notably,QDsADSC-Exos showed accumulation at the site of nerve injury in cases of ischemic peripheral nerve damage.Func⁃tional neurological assessments indicated that QDs-ADSC-Exos effectively promoted neural regeneration.This study highlights the potential of exosomes in treating ischemic peripheral nerve injuries and elucidates the spatio⁃temporal characteristics of exosome therapy,providing objective evidence for the further optimization of exosomebased treatment protocols.
文摘Exosomes,ubiquitously present in body fluids,serve as non-invasive biomarkers for disease diagnosis,monitoring,and treatment.As intercellular messengers,exosomes encapsulate a rich array of proteins,nucleic acids,and metabolites,although most studies have primarily focused on proteins and RNA.Recently,exosome metabolomics has demonstrated clinical value and potential advantages in disease detection and pathophysiology,despite significant challenges,particularly in exosome isolation and metabolite detection.This review discusses the significant technical challenges in exosome isolation and metabolite detection,highlighting the advancements in these areas that support the clinical application of exosome metabolomics,and illustrates the potential of exosomal metabolites from various body fluids as biomarkers for early disease diagnosis and treatment.
文摘目的:观察IL-12与IL-18联合刺激对树突状细胞(dendritic cell,DC)分泌的exosome(DC derived exosome,Dex)活性的影响,为探索高效的exosome肿瘤疫苗奠定基础。方法:取正常健康人外周血单个核细胞诱导培养DCs,分别以IL-12、IL-18或IL-12+IL-18联合刺激DC,并设空白对照组、T细胞对照组,分别提取各组的Dex,Western blotting检测Dex中HLA-DR、CD83的表达,流式细胞仪检测CD54、CD80及CD86的表达;MTT法检测Dex刺激T细胞增殖的作用,ELISA法测定T细胞IFN-γ的分泌量。结果:IL-12、IL-18、联合组、空白组的Dex中均有HLA-DR、CD83蛋白表达;联合组Dex的CD54(323.67±44.06 vs 246.17±31.91、236.33±33.87、167.67±28.73,P<0.05)、CD80(406.37±39.18 vs 331.67±36.15、335.67±41.38、260.00±35.58,P<0.05)及CD86(390.50±38.06 vs 314.33±36.64、319.00±33.10、246.83±30.55,P<0.05)表达均高于IL-12组、IL-18组及空白组;联合组刺激T细胞增殖高于IL-12组、IL-18组及空白组、T细胞组(1.98±0.31vs 1.55±0.23、1.57±0.21、1.10±0.18、0.53±0.09,P<0.05);联合组刺激T细胞分泌IFN-γ水平高于IL-12组、IL-18组及空白组、T细胞组(436.67±61.80 vs 295.04±40.25、358.18±55.77、225.00±36.44、139.50±17.63,P<0.05)。结论:IL-12与IL-18联合刺激能增加Dex表达CD54、CD80及CD86,促进Dex刺激的T细胞的增殖及其IFN-γ的分泌。
