目的探讨桂枝茯苓丸加味预防子宫内膜息肉术后复发的疗效及可能作用机制。方法将85例痰湿瘀结证子宫内膜息肉术后患者随机分为治疗组(43例)、对照组(42例),对照组给予地屈孕酮治疗,治疗组给予桂枝茯丸加味联合地屈孕酮治疗。治疗3个月...目的探讨桂枝茯苓丸加味预防子宫内膜息肉术后复发的疗效及可能作用机制。方法将85例痰湿瘀结证子宫内膜息肉术后患者随机分为治疗组(43例)、对照组(42例),对照组给予地屈孕酮治疗,治疗组给予桂枝茯丸加味联合地屈孕酮治疗。治疗3个月经周期,比较两组中医证候积分、Caspase-1/GSDMD信号通路[Gasdermin家族蛋白D(Gasdermin Family protein D,GSDMD)、半胱氨酸蛋白酶-1(caspase-1)、白细胞介素-1β(Interleukin-1β,IL-1β)、白细胞介素-18(Interleukin-18,IL-18)]、增殖和凋亡基因[增殖细胞核相关抗原Ki-67(Ki-67)、B细胞淋巴瘤/白血病-2基因(B-cell lymphoma/leukemia-2 gene,Bc1-2)]、临床疗效、不良反应及复发率。结果①治疗组经期延长/经量过多、经色紫黯/淋漓不尽、经行泄泻、经期出血、经行腹痛评分及中医证候总分低于对照组(P<0.05)。②治疗组子宫内膜组织GSDMD、Caspase-1、IL-1β、IL-18表达低于对照组(P<0.05)。③治疗组子宫内膜组织Ki-67、Bcl-2表达低于对照组(P<0.05)。④治疗组总有效率93.02%(40/43)高于对照组76.19%(32/42)(χ^(2)=4.647,P<0.05)。⑤治疗组不良反应(11.63%,5/43)、复发率(4.65%,2/43)低于对照组33.33%(14/42)、21.43%(9/42)(χ^(2)=5.767,5.308,P<0.05)。结论桂枝茯苓丸加味能够提高子宫内膜息肉术后患者临床疗效,减少不良反应及复发率,可能与抑制Caspase-1/GSDMD信号通路、拮抗炎症反应与下调细胞增殖和凋亡基因表达等因素有关。展开更多
Objective Stroke is a leading cause of death and disability worldwide,with ischemic stroke accounting for 80%-85%of cases.Despite the prevalence,effective treatments remain scarce.The compelling evidence suggest that ...Objective Stroke is a leading cause of death and disability worldwide,with ischemic stroke accounting for 80%-85%of cases.Despite the prevalence,effective treatments remain scarce.The compelling evidence suggest that high concentrations of ATP in the brain post-stroke can trigger irreversible neuronal damage and necrosis,contributing to a range of neurocellular dysfunctions.Pyroptosis,a recently identified form of programmed cell death,is characterized by caspase-1 activation and the action of the Gasdermin D(GSDMD)protein family,leading to cell perforation and inflammatory death.Methods In this study,human neuroblastoma SH-SY5Y cells were used to investigate the mechanisms of ATP-induced neurotoxicity and the protective effects of hydrogen sulfide(H_(2)S)against this toxicity through the antagonization of pyroptosis.We employed CCK-8 and LDH assays to assess cell viability.YO-PRO-1 fluorescent dyes and flow cytometry were conducted for detecting changes in cell membrane permeability.Western blot analysis was used to measure protein levels associated with cellular dysfunction.Results Our results indicate that high concentrations of ATP enhance cytotoxicity and increase cell membrane permeability in SH-SY5Y cells,that are mitigated by the H_(2)S donor NaHS.Furthermore,ATP was found to promote the activation of the NOD-like receptor pyrin domain-containing 1(NLRP-1),caspase-1,and the cleavage of GSDMD,with NaHS significantly attenuating these effects.Conclusion Our research suggests that H2S protects SH-SY5Y cells from ATP-induced neurotoxicity through a mechanism mediated by the NLRP1,caspase-1,and GSDMD pathway.展开更多
文摘目的探讨桂枝茯苓丸加味预防子宫内膜息肉术后复发的疗效及可能作用机制。方法将85例痰湿瘀结证子宫内膜息肉术后患者随机分为治疗组(43例)、对照组(42例),对照组给予地屈孕酮治疗,治疗组给予桂枝茯丸加味联合地屈孕酮治疗。治疗3个月经周期,比较两组中医证候积分、Caspase-1/GSDMD信号通路[Gasdermin家族蛋白D(Gasdermin Family protein D,GSDMD)、半胱氨酸蛋白酶-1(caspase-1)、白细胞介素-1β(Interleukin-1β,IL-1β)、白细胞介素-18(Interleukin-18,IL-18)]、增殖和凋亡基因[增殖细胞核相关抗原Ki-67(Ki-67)、B细胞淋巴瘤/白血病-2基因(B-cell lymphoma/leukemia-2 gene,Bc1-2)]、临床疗效、不良反应及复发率。结果①治疗组经期延长/经量过多、经色紫黯/淋漓不尽、经行泄泻、经期出血、经行腹痛评分及中医证候总分低于对照组(P<0.05)。②治疗组子宫内膜组织GSDMD、Caspase-1、IL-1β、IL-18表达低于对照组(P<0.05)。③治疗组子宫内膜组织Ki-67、Bcl-2表达低于对照组(P<0.05)。④治疗组总有效率93.02%(40/43)高于对照组76.19%(32/42)(χ^(2)=4.647,P<0.05)。⑤治疗组不良反应(11.63%,5/43)、复发率(4.65%,2/43)低于对照组33.33%(14/42)、21.43%(9/42)(χ^(2)=5.767,5.308,P<0.05)。结论桂枝茯苓丸加味能够提高子宫内膜息肉术后患者临床疗效,减少不良反应及复发率,可能与抑制Caspase-1/GSDMD信号通路、拮抗炎症反应与下调细胞增殖和凋亡基因表达等因素有关。
文摘Objective Stroke is a leading cause of death and disability worldwide,with ischemic stroke accounting for 80%-85%of cases.Despite the prevalence,effective treatments remain scarce.The compelling evidence suggest that high concentrations of ATP in the brain post-stroke can trigger irreversible neuronal damage and necrosis,contributing to a range of neurocellular dysfunctions.Pyroptosis,a recently identified form of programmed cell death,is characterized by caspase-1 activation and the action of the Gasdermin D(GSDMD)protein family,leading to cell perforation and inflammatory death.Methods In this study,human neuroblastoma SH-SY5Y cells were used to investigate the mechanisms of ATP-induced neurotoxicity and the protective effects of hydrogen sulfide(H_(2)S)against this toxicity through the antagonization of pyroptosis.We employed CCK-8 and LDH assays to assess cell viability.YO-PRO-1 fluorescent dyes and flow cytometry were conducted for detecting changes in cell membrane permeability.Western blot analysis was used to measure protein levels associated with cellular dysfunction.Results Our results indicate that high concentrations of ATP enhance cytotoxicity and increase cell membrane permeability in SH-SY5Y cells,that are mitigated by the H_(2)S donor NaHS.Furthermore,ATP was found to promote the activation of the NOD-like receptor pyrin domain-containing 1(NLRP-1),caspase-1,and the cleavage of GSDMD,with NaHS significantly attenuating these effects.Conclusion Our research suggests that H2S protects SH-SY5Y cells from ATP-induced neurotoxicity through a mechanism mediated by the NLRP1,caspase-1,and GSDMD pathway.
