在单因素试验的基础上,采用响应面方法对纤维素酶辅助提取沙葱总黄酮的工艺参数进行优化,得到最优工艺条件为提取时间4 h、提取温度39℃、体系p H 4.3,沙葱总黄酮提取量为5.48 mg/g。通过体外抗氧化及抗菌实验得出,此条件下提取所得沙...在单因素试验的基础上,采用响应面方法对纤维素酶辅助提取沙葱总黄酮的工艺参数进行优化,得到最优工艺条件为提取时间4 h、提取温度39℃、体系p H 4.3,沙葱总黄酮提取量为5.48 mg/g。通过体外抗氧化及抗菌实验得出,此条件下提取所得沙葱总黄酮具有较好的总还原能力、体外清除DPPH自由基及清除羟自由基的能力,对沙门氏菌具有较好的抑制作用,对大肠杆菌和金黄色葡萄球菌的抑制作用较弱,对绿脓杆菌无抑制作用。展开更多
The dynamic patterns of vitamin, fatty acid and amino acid content in the process of Allium mongolicum seed germination were investigated by using high performance liquid chromatography and high performance gas chroma...The dynamic patterns of vitamin, fatty acid and amino acid content in the process of Allium mongolicum seed germination were investigated by using high performance liquid chromatography and high performance gas chromatography, and the nutrition values of seedlings during 10 days of germinating process were also evaluated. A completely randomized design and ANOVA were employed in this study. The results showed that: 1) Compared with control（dry seed）, the contents of VB2, VB5 and carotenoid at different germinating stages were significantly increased, whereas the content of Vc has no significant difference and remained at high level ; 2) The content of various amino acids varied at different germinating stages, while the content of total amino acid and required amino acids significantly increased. The content of glutamic acid was the highest in all of amino acids, with arginine and aspartate followed; 3) The components of fatty acids presented various dynamic changes at different germinating stages. However, the content of total saturated fatty acid increased, while that of the total polyunsaturated fatty acid decreased. The results indicated that the nutrient value of the seedling was higher than that of the seed, due to higher contents of Vc, VB2, VB5,carotenoid, glutamate and PUFA in the seedlings. Therefore, 6-10 days old seedlings of A. mongolicum is suggested to be exploit as an esculent because it contains abundant vitamins and amino acids for the benefit of human body.展开更多
文摘在单因素试验的基础上,采用响应面方法对纤维素酶辅助提取沙葱总黄酮的工艺参数进行优化,得到最优工艺条件为提取时间4 h、提取温度39℃、体系p H 4.3,沙葱总黄酮提取量为5.48 mg/g。通过体外抗氧化及抗菌实验得出,此条件下提取所得沙葱总黄酮具有较好的总还原能力、体外清除DPPH自由基及清除羟自由基的能力,对沙门氏菌具有较好的抑制作用,对大肠杆菌和金黄色葡萄球菌的抑制作用较弱,对绿脓杆菌无抑制作用。
文摘The dynamic patterns of vitamin, fatty acid and amino acid content in the process of Allium mongolicum seed germination were investigated by using high performance liquid chromatography and high performance gas chromatography, and the nutrition values of seedlings during 10 days of germinating process were also evaluated. A completely randomized design and ANOVA were employed in this study. The results showed that: 1) Compared with control（dry seed）, the contents of VB2, VB5 and carotenoid at different germinating stages were significantly increased, whereas the content of Vc has no significant difference and remained at high level ; 2) The content of various amino acids varied at different germinating stages, while the content of total amino acid and required amino acids significantly increased. The content of glutamic acid was the highest in all of amino acids, with arginine and aspartate followed; 3) The components of fatty acids presented various dynamic changes at different germinating stages. However, the content of total saturated fatty acid increased, while that of the total polyunsaturated fatty acid decreased. The results indicated that the nutrient value of the seedling was higher than that of the seed, due to higher contents of Vc, VB2, VB5,carotenoid, glutamate and PUFA in the seedlings. Therefore, 6-10 days old seedlings of A. mongolicum is suggested to be exploit as an esculent because it contains abundant vitamins and amino acids for the benefit of human body.
