3-Oxo-11,12,13-trihydroxyeudesm-4-ene(1) was a highly oxygenated natural eudesmane isolated from traditional herbal medicine with an antiphlogostic and spasmolytic activity.For the purpose of pharmacological activity ...3-Oxo-11,12,13-trihydroxyeudesm-4-ene(1) was a highly oxygenated natural eudesmane isolated from traditional herbal medicine with an antiphlogostic and spasmolytic activity.For the purpose of pharmacological activity research on natural product 1 and its derivatives,a facile total synthesis of compound 1 starting from(+)-dihydrocarvone(2) was completed in an overall yield of 24%.The structures of all intermediates and product 1 were confirmed via 1H NMR,13C NMR,MS and IR techniques.The NMR data of compound 1 are in agreement with those of natural products.展开更多
文摘3-Oxo-11,12,13-trihydroxyeudesm-4-ene(1) was a highly oxygenated natural eudesmane isolated from traditional herbal medicine with an antiphlogostic and spasmolytic activity.For the purpose of pharmacological activity research on natural product 1 and its derivatives,a facile total synthesis of compound 1 starting from(+)-dihydrocarvone(2) was completed in an overall yield of 24%.The structures of all intermediates and product 1 were confirmed via 1H NMR,13C NMR,MS and IR techniques.The NMR data of compound 1 are in agreement with those of natural products.
文摘目的·建立人血浆中7α-羟基-4-胆固醇烯-3-酮(7α-hydroxy-4-cholesten-3-one,C4)以及胆酸(cholic acid,CA)、鹅去氧胆酸(chenodeoxycholic acid,CDCA)的定量方法。方法·采用乙腈提取人血浆中C4、CA、CDCA,利用超高效液相色谱-串联质谱(ultra-high performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)技术,通过内标校正的标准曲线法定量C4、CA、CDCA含量。结果·经方法学验证,C4、CA、CDCA的检出限(limits of detection,LOD)分别为0.16、0.02、0.04 nmol/L,均具有良好的线性关系(均R2>0.998);重复性的相对标准偏差(relative standard deviation,RSD)均低于3.0%;日内及日间精密度的RSD均小于6%;4℃保存7 d结果稳定,RSD均小于10%;C4、CA和CDCA的平均加标回收率分别为97.7%、113.3%、105.0%。结论·该方法具有较高的检测灵敏度、良好的精密度和稳定性,可用于血浆生物样本的定量检测。
