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产ESBL克雷伯菌与大肠埃希菌质粒分布的初步研究 被引量:2
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作者 马佳毓 张文莉 +2 位作者 付英梅 赵月辉 叶杨芹 《微生物学杂志》 CAS CSCD 2003年第2期33-34,共2页
比较产超广谱 β 内酰胺酶克雷伯菌与大肠埃希菌质粒的表型与分布。用BlaTEM引物经PCR技术将 5 1株克雷伯菌与 2 9株大肠埃希菌编码产超广谱 β 内酰胺酶的质粒扩增 ,琼脂糖凝胶电泳分离目的片段后得到完整的质粒电泳图谱。克雷伯菌与... 比较产超广谱 β 内酰胺酶克雷伯菌与大肠埃希菌质粒的表型与分布。用BlaTEM引物经PCR技术将 5 1株克雷伯菌与 2 9株大肠埃希菌编码产超广谱 β 内酰胺酶的质粒扩增 ,琼脂糖凝胶电泳分离目的片段后得到完整的质粒电泳图谱。克雷伯菌与大肠埃希菌存在有相同大小的产超广谱 β 内酰胺酶的质粒。 2种细菌有产ESBL相同质粒 ,PCR技术可用于产超广谱 β 内酰胺酶细菌质粒的分析。 展开更多
关键词 克雷伯菌 大肠埃希菌 质粒分布 Β-内酰胺类抗生素 耐药性 耐药质粒
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Simulation of alumina dissolution and temperature response under different feeding quantities in aluminum reduction cell 被引量:2
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作者 LI Si-yun LI Mao +2 位作者 HOU Wen-yuan LI He-song CHENG Ben-jun 《Journal of Central South University》 SCIE EI CAS CSCD 2019年第8期2119-2128,共10页
In the feeding process of aluminum electrolytic, feeding quantity of alumina affects eventually dissolved quantity at the end of a feeding cycle. Based on the OpenFOAM platform, dissolution model coupled with heat and... In the feeding process of aluminum electrolytic, feeding quantity of alumina affects eventually dissolved quantity at the end of a feeding cycle. Based on the OpenFOAM platform, dissolution model coupled with heat and mass transfer was established. Applying the Rosin-Rammler function, alumina particle size distribution under different feeding quantities was obtained. The temperature response of electrolyte after feeding was included and calculated, and the dissolution processes of alumina with different feeding quantities (0.6, 0.8, 1.0, 1.2, 1.4, 1.6 kg) after feeding were simulated in 300 kA aluminum reduction cell. The results show that with the increase of feeding quantity, accumulated mass fraction of dissolved alumina decreases, and the time required for the rapid dissolution stage extends. When the feeding quantity is 0.6 kg and 1.2 kg, it takes the shortest time for the electrolyte temperature dropping before rebounding back. With the increase of feeding quantity, the dissolution rate in the rapid dissolution stage increases at first and then decreases gradually. The most suitable feeding quantity is 1.2 kg. The fitting equation of alumina dissolution curve under different feeding quantities is obtained, which can be used to evaluate the alumina dissolution and guide the feeding quantity and feeding cycle. 展开更多
关键词 alumina dissolution heat and mass transfer particle size distribution temperature response NUMERICAL
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Probabilistic analysis of geomembrane puncture from granular material under liquid pressure 被引量:1
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作者 姜晓桢 束一鸣 朱俊高 《Journal of Central South University》 SCIE EI CAS 2013年第11期3256-3264,共9页
A simplified probabilistic analysis of geomembrane punctures from granular material was presented when subjected to liquid pressure.The probability distribution of contact force between geomembrane and granular materi... A simplified probabilistic analysis of geomembrane punctures from granular material was presented when subjected to liquid pressure.The probability distribution of contact force between geomembrane and granular material was obtained based on the principle of equal probability and assumptions that grains are spheres with constant size.A particle flow code PFC3Dwas employed to simulate the contact process which indicates a good agreement with the theoretical probabilistic analysis.The odds of geomembrane puncture from grains of constant size were obtained by evaluating the puncture force which should not exceed the puncture resistance of geomembrane.The effects of grain radius,grain rigidity and liquid pressure were studied in more detail and displayed in graphs.Both high-level of liquid pressure and large grain can result in high risk of geomembrane puncture.The influence of grain rigidity on the geomembrane puncture odds is significant.For granular material with a grain size distribution,the geomembrane puncture odds can be estimated by the grain size distribution,served as weight function and it is a cautious design if the largest grain is chosen as the design grain size. 展开更多
关键词 geomembrane puncture probability distribution contact force
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