本试验旨在研究苜蓿草粉来源的不可溶性纤维对生长猪结肠微生物区系及其发酵代谢产物的影响。试验选用24头(24.8±0.7)kg的杜×长×大去势公猪,随机分为4组,分别饲喂含0(对照组)、5%、10%和15%苜蓿草粉的饲粮,每组6个重复,...本试验旨在研究苜蓿草粉来源的不可溶性纤维对生长猪结肠微生物区系及其发酵代谢产物的影响。试验选用24头(24.8±0.7)kg的杜×长×大去势公猪,随机分为4组,分别饲喂含0(对照组)、5%、10%和15%苜蓿草粉的饲粮,每组6个重复,每个重复1头猪。试验28 d后屠宰取样,测定血清脂肪酸组成、结肠食糜短链脂肪酸浓度及微生物组成。结果发现:饲喂苜蓿草粉显著降低了生长猪的料重比(P<0.05),但对平均日增重和平均日采食量无显著影响(P>0.05);结肠微生物菌群16S r DNA V3区测序结果表明,苜蓿草粉对猪结肠微生物区系无显著影响(P>0.05),但显著增加了结肠食糜中总短链脂肪酸、乙酸和丁酸的浓度(P<0.05);随着饲粮中苜蓿草粉含量的增加,血清多不饱和脂肪酸含量显著提高(P<0.05),而饱和脂肪酸和单不饱和脂肪酸含量显著下降(P<0.05)。结果提示:苜蓿草粉来源的不可溶性纤维不影响结肠微生物菌群结构,但增强了结肠微生物的发酵活动和短链脂肪酸的产生,具有调节猪机体脂肪酸组成的作用。展开更多
Objective Ulcerative colitis is a prevalent immunoinflammatory disease.Th17/Treg cell imbalance and gut microbiota dysregulation are key factors in ulcerative colitis pathogenesis.The actin cytoskeleton contributes to...Objective Ulcerative colitis is a prevalent immunoinflammatory disease.Th17/Treg cell imbalance and gut microbiota dysregulation are key factors in ulcerative colitis pathogenesis.The actin cytoskeleton contributes to regulating the proliferation,differentiation,and migration of Th17 and Treg cells.Wdr63,a gene containing the WD repeat domain,participates in the structure and functional modulation of actin cytoskeleton.Recent research indicates that WDR63 may serve as a regulator of cell migration and metastasis via actin polymerization inhibition.This article aims to explore the effect of Wdr63 deletion on Th17/Treg cells and ulcerative colitis.Methods We constructed Wdr63-/-mice,induced colitis in mice using dextran sulfate sodium salt,collected colon tissue for histopathological staining,collected mesenteric lymph nodes for flow cytometry analysis,and collected healthy mouse feces for microbial diversity detection.Results Compared with wild-type colitis mice,Wdr63-/-colitis mice had a more pronounced shortening of colonic tissue,higher scores on disease activity index and histological damage index,Treg cells decreased and Th17 cells increased in colonic tissue and mesenteric lymph nodes,a lower level of anti-inflammatory cytokine IL-10,and a higher level of pro-inflammatory cytokine IL-17A.In addition,WDR63 has shown positive effects on maintaining intestinal microbiota homeostasis.It maintains the balance of Bacteroidota and Firmicutes,promoting the formation of beneficial intestinal bacteria linked to immune inflammation.Conclusion Wdr63 deletion aggravates ulcerative colitis in mice,WDR63 inhibits colonic inflammation likely by regulating Th17/Treg balance and maintains intestinal microbiota homeostasis.展开更多
文摘本试验旨在研究苜蓿草粉来源的不可溶性纤维对生长猪结肠微生物区系及其发酵代谢产物的影响。试验选用24头(24.8±0.7)kg的杜×长×大去势公猪,随机分为4组,分别饲喂含0(对照组)、5%、10%和15%苜蓿草粉的饲粮,每组6个重复,每个重复1头猪。试验28 d后屠宰取样,测定血清脂肪酸组成、结肠食糜短链脂肪酸浓度及微生物组成。结果发现:饲喂苜蓿草粉显著降低了生长猪的料重比(P<0.05),但对平均日增重和平均日采食量无显著影响(P>0.05);结肠微生物菌群16S r DNA V3区测序结果表明,苜蓿草粉对猪结肠微生物区系无显著影响(P>0.05),但显著增加了结肠食糜中总短链脂肪酸、乙酸和丁酸的浓度(P<0.05);随着饲粮中苜蓿草粉含量的增加,血清多不饱和脂肪酸含量显著提高(P<0.05),而饱和脂肪酸和单不饱和脂肪酸含量显著下降(P<0.05)。结果提示:苜蓿草粉来源的不可溶性纤维不影响结肠微生物菌群结构,但增强了结肠微生物的发酵活动和短链脂肪酸的产生,具有调节猪机体脂肪酸组成的作用。
文摘Objective Ulcerative colitis is a prevalent immunoinflammatory disease.Th17/Treg cell imbalance and gut microbiota dysregulation are key factors in ulcerative colitis pathogenesis.The actin cytoskeleton contributes to regulating the proliferation,differentiation,and migration of Th17 and Treg cells.Wdr63,a gene containing the WD repeat domain,participates in the structure and functional modulation of actin cytoskeleton.Recent research indicates that WDR63 may serve as a regulator of cell migration and metastasis via actin polymerization inhibition.This article aims to explore the effect of Wdr63 deletion on Th17/Treg cells and ulcerative colitis.Methods We constructed Wdr63-/-mice,induced colitis in mice using dextran sulfate sodium salt,collected colon tissue for histopathological staining,collected mesenteric lymph nodes for flow cytometry analysis,and collected healthy mouse feces for microbial diversity detection.Results Compared with wild-type colitis mice,Wdr63-/-colitis mice had a more pronounced shortening of colonic tissue,higher scores on disease activity index and histological damage index,Treg cells decreased and Th17 cells increased in colonic tissue and mesenteric lymph nodes,a lower level of anti-inflammatory cytokine IL-10,and a higher level of pro-inflammatory cytokine IL-17A.In addition,WDR63 has shown positive effects on maintaining intestinal microbiota homeostasis.It maintains the balance of Bacteroidota and Firmicutes,promoting the formation of beneficial intestinal bacteria linked to immune inflammation.Conclusion Wdr63 deletion aggravates ulcerative colitis in mice,WDR63 inhibits colonic inflammation likely by regulating Th17/Treg balance and maintains intestinal microbiota homeostasis.
