目的探讨白细胞介素1受体拮抗剂基因内舍子2的变数串联重复（VNTR）多态性与老年缺血性脑卒中的关系。方法选择老年缺血性脑卒中患者112例作为脑卒中组，选择同期年龄和性别相匹配的健康志愿者或其他疾病住院的患者105例作为对照组，通...目的探讨白细胞介素1受体拮抗剂基因内舍子2的变数串联重复（VNTR）多态性与老年缺血性脑卒中的关系。方法选择老年缺血性脑卒中患者112例作为脑卒中组，选择同期年龄和性别相匹配的健康志愿者或其他疾病住院的患者105例作为对照组，通过PCR和琼脂糖凝胶电泳来确定其基因型。结果脑卒中组患者A2等位基因频率低于对照组（4．9％ vs 10．5％，X^2=4．78．P=0．029），A1A2＋A2A2基因型的频率也较对照组低（9．8％ vs 20．0％．）[X^2=4．47，P=0．035）。结论白细胞介素1受体拮抗剂内含子2基因VNTR多态性与老年缺血性脑卒中有关，其A2等位基因对脑卒中可能有保护作用。展开更多
Objective:To study the role of IL 2Rγ chain in the immuno senescence process Methods:8 young, aged from 18 to 35 years(average 24 9±5 8 years), and 17 old healthy people, aged from 65 to 79 years(average 69 4...Objective:To study the role of IL 2Rγ chain in the immuno senescence process Methods:8 young, aged from 18 to 35 years(average 24 9±5 8 years), and 17 old healthy people, aged from 65 to 79 years(average 69 4±4 2 years), had been selected for determination the proliferative responses of peripheral blood lymphocytes to PHA, the percentage and dynamic changes of CD132, CD45R positive cells, also, the gene expression of IL 2Rγ chain with RT PCR using the designed primers Results:The results showed that the PHA proliferative response of lymphocytes of aged people(0 199±0 055) were lower than that of young people(0 279±0 054), P=0 0012 The expression of IL 2Rγ chain on the lymphocytes surface decreased in aged people, 46 2%±7 7%, compared to young people, 68 0%±11 8%, P<0 001, It was verified from RT PCR result that the event occurred at transcription level The time dynamic curve of IL 2Rγ chain expression were different between aged and young people Conclusion:A preliminary conclusion would be drawn that the IL 2Rγ chain play an important role in the declined immune function of aged展开更多
目的:S100钙结合蛋白A9(S100A9)激活核因子κB(NF-κB)促进小胶质细胞toll样受体7(TLR7)的表达和炎症因子释放的作用及其机制研究。方法:CCK-8实验检测BV2小胶质细胞的增殖率;转录组测序并结合GO分析、KEGG富集分析和STRING数据库对差...目的:S100钙结合蛋白A9(S100A9)激活核因子κB(NF-κB)促进小胶质细胞toll样受体7(TLR7)的表达和炎症因子释放的作用及其机制研究。方法:CCK-8实验检测BV2小胶质细胞的增殖率;转录组测序并结合GO分析、KEGG富集分析和STRING数据库对差异基因(DEGs)进行比对并从差异表达基因中筛选出目标基因;Real time RT-PCR验证TLR7的表达;免疫荧光染色检测CD68、CD206的表达;Western Blot检测CD68、CD206、TLR7、p65、p-p65的表达;ELISA检测白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达。结果:中等浓度的S100A9对小胶质细胞无增殖抑制效应;实验组CD68蛋白的表达水平较对照组明显增加,而CD206蛋白的表达水平明显下降,提示S100A9促进BV2小胶质细胞向促炎型激活;Toll样受体4(TLR4)的抑制剂TAK-242明显抑制S100A9刺激BV2小胶质细胞后TNF-α和IL-6的表达水平;TLR4/NF-κB通路激活促进TLR7蛋白表达。结论:中等浓度的S100A9可以促进小胶质细胞向促炎型极化,通过激活TLR4/NF-κB通路促进TLR7表达和包括TNF-α和IL-6在内的多种炎症因子的释放,S100A9具有明显的促炎作用。展开更多
文摘目的探讨白细胞介素1受体拮抗剂基因内舍子2的变数串联重复（VNTR）多态性与老年缺血性脑卒中的关系。方法选择老年缺血性脑卒中患者112例作为脑卒中组，选择同期年龄和性别相匹配的健康志愿者或其他疾病住院的患者105例作为对照组，通过PCR和琼脂糖凝胶电泳来确定其基因型。结果脑卒中组患者A2等位基因频率低于对照组（4．9％ vs 10．5％，X^2=4．78．P=0．029），A1A2＋A2A2基因型的频率也较对照组低（9．8％ vs 20．0％．）[X^2=4．47，P=0．035）。结论白细胞介素1受体拮抗剂内含子2基因VNTR多态性与老年缺血性脑卒中有关，其A2等位基因对脑卒中可能有保护作用。
文摘Objective:To study the role of IL 2Rγ chain in the immuno senescence process Methods:8 young, aged from 18 to 35 years(average 24 9±5 8 years), and 17 old healthy people, aged from 65 to 79 years(average 69 4±4 2 years), had been selected for determination the proliferative responses of peripheral blood lymphocytes to PHA, the percentage and dynamic changes of CD132, CD45R positive cells, also, the gene expression of IL 2Rγ chain with RT PCR using the designed primers Results:The results showed that the PHA proliferative response of lymphocytes of aged people(0 199±0 055) were lower than that of young people(0 279±0 054), P=0 0012 The expression of IL 2Rγ chain on the lymphocytes surface decreased in aged people, 46 2%±7 7%, compared to young people, 68 0%±11 8%, P<0 001, It was verified from RT PCR result that the event occurred at transcription level The time dynamic curve of IL 2Rγ chain expression were different between aged and young people Conclusion:A preliminary conclusion would be drawn that the IL 2Rγ chain play an important role in the declined immune function of aged
文摘目的:S100钙结合蛋白A9(S100A9)激活核因子κB(NF-κB)促进小胶质细胞toll样受体7(TLR7)的表达和炎症因子释放的作用及其机制研究。方法:CCK-8实验检测BV2小胶质细胞的增殖率;转录组测序并结合GO分析、KEGG富集分析和STRING数据库对差异基因(DEGs)进行比对并从差异表达基因中筛选出目标基因;Real time RT-PCR验证TLR7的表达;免疫荧光染色检测CD68、CD206的表达;Western Blot检测CD68、CD206、TLR7、p65、p-p65的表达;ELISA检测白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达。结果:中等浓度的S100A9对小胶质细胞无增殖抑制效应;实验组CD68蛋白的表达水平较对照组明显增加,而CD206蛋白的表达水平明显下降,提示S100A9促进BV2小胶质细胞向促炎型激活;Toll样受体4(TLR4)的抑制剂TAK-242明显抑制S100A9刺激BV2小胶质细胞后TNF-α和IL-6的表达水平;TLR4/NF-κB通路激活促进TLR7蛋白表达。结论:中等浓度的S100A9可以促进小胶质细胞向促炎型极化,通过激活TLR4/NF-κB通路促进TLR7表达和包括TNF-α和IL-6在内的多种炎症因子的释放,S100A9具有明显的促炎作用。
