In this paper,the noncovalent interactions between α1-acid glycoprotein（AAG） and the drugs,such as ketoprofen（KP） and anisodine hydrobromide（AN） were studied by using electrospray ion trap mass spectrometry met...In this paper,the noncovalent interactions between α1-acid glycoprotein（AAG） and the drugs,such as ketoprofen（KP） and anisodine hydrobromide（AN） were studied by using electrospray ion trap mass spectrometry method.The results show that both of the drugs had a strong coalescent interaction with AAG,the（dissociation） constants（Kd） and the apparent stoichiometric ratio of noncovalent complexes AN-AAG were（1.75×10-3 mol/L） and 1∶7,and those for KP-AAG complex were 4.95×10-4 mol/L and 1∶4,respectively.According to the thermodynamic parameters of the noncoralent complexes,the main sorts of binding force were static-electricity gravitation for KP-AAG and Van der Waals force for AN-AAG,respectively. The experiment proves that ESI-MS technology as a new method for studying the drug-protein noncoralent binding can offer some advantages in sensitivity,speed,accuracy and directness particularly in the determination of the stoichiormetry of the complex compared to other well-established methods such as circular dichroism and fluorescence spectroscopy,etc.展开更多
Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with ...Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.展开更多
湖北小连翘(Hypericum hubeiense L.H.Wu et D.P.Yang)是2004年在我国发现的一个金丝桃属(Hyperi-cum)植物(藤黄科)新种。利用高效液相色谱与质谱联用技术对该植物中主要化学成分进行了分离分析,初步鉴定出金丝桃素类、黄酮类等12种化...湖北小连翘(Hypericum hubeiense L.H.Wu et D.P.Yang)是2004年在我国发现的一个金丝桃属(Hyperi-cum)植物(藤黄科)新种。利用高效液相色谱与质谱联用技术对该植物中主要化学成分进行了分离分析,初步鉴定出金丝桃素类、黄酮类等12种化学成分。同时,以金丝桃素为外标,采用HPLC法在590nm波长下测定了金丝桃素类成分的含量。展开更多
文摘In this paper,the noncovalent interactions between α1-acid glycoprotein（AAG） and the drugs,such as ketoprofen（KP） and anisodine hydrobromide（AN） were studied by using electrospray ion trap mass spectrometry method.The results show that both of the drugs had a strong coalescent interaction with AAG,the（dissociation） constants（Kd） and the apparent stoichiometric ratio of noncovalent complexes AN-AAG were（1.75×10-3 mol/L） and 1∶7,and those for KP-AAG complex were 4.95×10-4 mol/L and 1∶4,respectively.According to the thermodynamic parameters of the noncoralent complexes,the main sorts of binding force were static-electricity gravitation for KP-AAG and Van der Waals force for AN-AAG,respectively. The experiment proves that ESI-MS technology as a new method for studying the drug-protein noncoralent binding can offer some advantages in sensitivity,speed,accuracy and directness particularly in the determination of the stoichiormetry of the complex compared to other well-established methods such as circular dichroism and fluorescence spectroscopy,etc.
文摘Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.
文摘湖北小连翘(Hypericum hubeiense L.H.Wu et D.P.Yang)是2004年在我国发现的一个金丝桃属(Hyperi-cum)植物(藤黄科)新种。利用高效液相色谱与质谱联用技术对该植物中主要化学成分进行了分离分析,初步鉴定出金丝桃素类、黄酮类等12种化学成分。同时,以金丝桃素为外标,采用HPLC法在590nm波长下测定了金丝桃素类成分的含量。
