An electrochemiluminescence(ECL) biosensor based on solid phase antigen competitive immunoreactions was constructed to determine methsulfuron methyl using Ru(bpy) 2+ 3 as labeling reagent. The coating hapten was bound...An electrochemiluminescence(ECL) biosensor based on solid phase antigen competitive immunoreactions was constructed to determine methsulfuron methyl using Ru(bpy) 2+ 3 as labeling reagent. The coating hapten was bound to the surface of microparticles through avitin biotin reaction. An electrochemical flow cell containing working electrodes and counter electrodes for initiation of the ECL reaction was constructed. Results showed that the detection limit for methsulfuron methyl was 0.1 ng/mL. [WT5HZ]展开更多
以磁性微球固定相思子毒素多抗制备捕获探针,以三联吡啶钌标记相思子毒素单抗作为发光探针,两者与相思子毒素发生特异性免疫反应形成夹心复合物,成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素,浓度在0.1...以磁性微球固定相思子毒素多抗制备捕获探针,以三联吡啶钌标记相思子毒素单抗作为发光探针,两者与相思子毒素发生特异性免疫反应形成夹心复合物,成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素,浓度在0.1～1000μg/L范围内与电化学发光强度呈良好的对数线性关系,拟合方程为lg Y=0.763 lg X+0.562(R=0.9903,N=7,P<0.0001),检出限为0.1μg/L。展开更多
基金Thanks for the financial supports from the Natural Sciences and Engineering Research Council of Canada of Canada(NSERC,DG RGPIN-2013-201697(Z.D.)RGPIN-2018-06556(Z.D.))+6 种基金Canada Foundation of Innovation/Ontario Innovation Trust(CFI/OIT,9040(Z.D.))Premier's Research Excellence Award(PREA,2003(Z.D.))Canada Institute of Photonics Innovation(2005(Z.D.))Ontario Photonics Consortium(2002 Z.D.)National Natural Science Foundation of China(22004034(X.Q.))Natural Science Foundation of Hunan Province(China)(2020JJ5226(X.Q.))China Scholarship Council(CSC,201908430010(X.Q.)).
文摘An electrochemiluminescence(ECL) biosensor based on solid phase antigen competitive immunoreactions was constructed to determine methsulfuron methyl using Ru(bpy) 2+ 3 as labeling reagent. The coating hapten was bound to the surface of microparticles through avitin biotin reaction. An electrochemical flow cell containing working electrodes and counter electrodes for initiation of the ECL reaction was constructed. Results showed that the detection limit for methsulfuron methyl was 0.1 ng/mL. [WT5HZ]
文摘以磁性微球固定相思子毒素多抗制备捕获探针,以三联吡啶钌标记相思子毒素单抗作为发光探针,两者与相思子毒素发生特异性免疫反应形成夹心复合物,成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素,浓度在0.1～1000μg/L范围内与电化学发光强度呈良好的对数线性关系,拟合方程为lg Y=0.763 lg X+0.562(R=0.9903,N=7,P<0.0001),检出限为0.1μg/L。
